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Showing: 741 - 760 of 13978 results

  1. pCMV-T7-SpCas9-P2A-EGFP (RTW3027)

    Plasmid
    #139987
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized SpCas9 with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Walton et al Science. 2020 Apr 17;368(6488):290-296.
    Insert
    human codon optimized SpCas9 with BPNLS-3xFLAG-P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Promoter
    CMV and T7
    Available Since
    March 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    139987-plasmid-map-sequence-id-275335

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  2. pcDNA GNSTM-3-Flag-10-Lamp2b-HA

    Plasmid
    #71293
    Purpose
    Encodes (N to C): GNSTM glycosylation motif, 3 residue spacer, Flag tag,10 residue spacer, Lamp2b (exosomal transmembrane protein), 3 residue spacer, HA tag.
    Depositor
    Joshua Leonard
    Article
    Hung et al J Biol Chem. 2015 Mar 27;290(13):8166-72
    Insert
    Lamp2b (LAMP2 Human)
    Tags
    FLAG, GNSTM glycosylation motif, HA, and Lamp2 si…
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Dec. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    71293-plasmid-map-sequence-id-127936

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  4. ELONGIN BC (XX01TCEB1A-c001)

    Plasmid
    #110274
    Purpose
    Elongin B and Elongin C co-expression plasmid for structure determination; Full length Elongin B & amino acids 17-112 of Elogin C
    Depositor
    Nicola Burgess-Brown
    Insert
    Elongin BC (ELOC, ELOB Human)
    Expression
    Bacterial
    Mutation
    Elongin B is full length. Elongin C contains a.a…
    Promoter
    T7
    Available Since
    May 31, 2018
    Availability
    Academic Institutions and Nonprofits only
    110274-plasmid-map-sequence-id-209817

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  16. Lenti-(BB)-EF1a-KRAB-dCas9-P2A-EGFP

    Plasmid
    #118156
    Purpose
    Catalytically inactive Cas9 from S. pyogenes with P2A-EGFP under the EF1a core promoter, and cloning backbone for sgRNA. Contains BsmBI sites for insertion of spacer sequences.
    Depositor
    Jorge Ferrer
    Insert
    KRAB-dCas9-P2A-EGFP
    Use
    CRISPR and Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Promoter
    EF1a core and U6
    Available Since
    April 1, 2019
    Availability
    Academic Institutions and Nonprofits only
    118156-plasmid-map-sequence-id-239644

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Showing: 741 - 760 of 13978 results