We narrowed to 32,058 results for: eng

1. pTS1024-Tier1-PRPBSA-YPet-p2A-PuroR

   Plasmid

   #169499

   Purpose

   Tier-1 vector encoding PRPBSA-driven YPet in combination with a marker for puromycine selection (PRPBSA-YPet-p2A-PuroR-pA)

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PRPBSA-driven YPet-P2aA-PuroR
   

   Expression

   Mammalian

   Promoter

   PRBSA

   Available Since

   June 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTS1047-Tier1-PhCMV-YPet-p2A-PuroR

   Plasmid

   #169498

   Purpose

   Tier-1 vector encoding PhCMV-driven YPet in combination with a marker for puromycin selection (PhCMV-YPet-p2A-PuroR-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PCMV-driven YPet-P2aA-PuroR
   

   Expression

   Mammalian

   Promoter

   PhCMV

   Available Since

   June 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTS1023-Tier1-PhCMV-p2A-PuroR

   Plasmid

   #169497

   Purpose

   Tier-1 vector encoding PhCMV-driven p2A sequence fused to a marker for puromycine selection (PhCMV-p2A-PuroR-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PCMV-driven P2A-PuroR
   

   Expression

   Mammalian

   Promoter

   PhCMV

   Available Since

   June 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pVH9-Tier1-PhCMV-3xNLS

   Plasmid

   #169478

   Purpose

   Tier-1 vector encoding PhCMV-driven triple nuclear localization signal expression (PhCMV-3xNLS-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PCMV-driven nuclear localization signal
   

   Expression

   Mammalian

   Promoter

   PhCMV

   Available Since

   June 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCDF-pntAB

   Plasmid

   #158609

   Purpose

   Low phosphate induction of pntAB

   Depositor

   Michael Lynch

   Article

   Li et al Metab Eng. 2021 Jan 15. pii: S1096-7176(

   Insert

   pntAB(membrane bound proton translocating pyridine nucleotide transhydrogenase)
   

   Expression

   Bacterial

   Promoter

   Insulated ugpBp from E. coli

   Available Since

   Jan. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pPPC031

   Plasmid

   #171152

   Purpose

   pGNW2 derivative with integration site at P. putida prophage1 for integration of J3-BBa_J23117-mRFP cassette

   Depositor

   Jesse Zalatan

   Article

   Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(

   Insert

   J3-BBa_J23117-mRFP
   

   Expression

   Bacterial

   Promoter

   J3-BBa_J23117

   Available Since

   Dec. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pPPC034

   Plasmid

   #171153

   Purpose

   pGNW2 derivative with integration site at P. putida prophage2 for integration of J3(106)-BBa_J23111-sfGFP cassette

   Depositor

   Jesse Zalatan

   Article

   Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(

   Insert

   J3(106)- BBa_J23111-sfGFP
   

   Expression

   Bacterial

   Promoter

   J3(106)-BBa_J23111

   Available Since

   Dec. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pPPC018.AAV

   Plasmid

   #171146

   Purpose

   Expression of J1-BBa_J23117-mRFP and hAAVS1 scRNA on pRK2-GmR plasmid

   Depositor

   Jesse Zalatan

   Article

   Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(

   Inserts

   hAAVS1 scRNA
   J1-BBa_J23117-mRFP
   

   Use

   CRISPR

   Expression

   Bacterial

   Promoter

   BBa_J23119 and J1-BBa_J23117

   Available Since

   Oct. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pPPC016.109

   Plasmid

   #171144

   Purpose

   Expression of J1-BBa_J23117-mRFP and J109 scRNA on pBBR1-GmR plasmid

   Depositor

   Jesse Zalatan

   Article

   Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(

   Inserts

   J109 scRNA
   J1-BBa_J23117-mRFP
   

   Use

   CRISPR

   Expression

   Bacterial

   Promoter

   BBa_J23119 and J1-BBa_J23117

   Available Since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCTCON2-BXG-altTAG

    Plasmid

    #158132

    Purpose

    Dual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (TAG construct)

    Depositor

    James Van Deventer

    Article

    Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

    Insert

    BFP-GFP fragment
    

    Expression

    Yeast

    Mutation

    Cloned in a TAG codon at the first serine residue…

    Promoter

    GAL1-10

    Available Since

    Jan. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCTCON2-BXG-altTAG2

    Plasmid

    #158134

    Purpose

    Dual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (TAG construct)

    Depositor

    James Van Deventer

    Article

    Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

    Insert

    BFP-GFP fragment
    

    Expression

    Yeast

    Mutation

    Cloned in a TAG codon at the second serine residu…

    Promoter

    GAL1-10

    Available Since

    Jan. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCTCON2-BXG-altTAG3

    Plasmid

    #158136

    Purpose

    Dual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (TAG construct)

    Depositor

    James Van Deventer

    Article

    Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

    Insert

    BFP-GFP fragment
    

    Expression

    Yeast

    Mutation

    Cloned in a TAG codon at the third alanine residu…

    Promoter

    GAL1-10

    Available Since

    Jan. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCTCON2-BXG-altTAG4

    Plasmid

    #158137

    Purpose

    Dual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (TAG construct)

    Depositor

    James Van Deventer

    Article

    Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

    Insert

    BFP-GFP fragment
    

    Expression

    Yeast

    Mutation

    Cloned in a TAG codon at the third serine residue…

    Promoter

    GAL1-10

    Available Since

    Jan. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pILGFPJ1

    Plasmid

    #83561

    Purpose

    Express yEGFP-ERG9 ER signal peptide under the control of TEF1 promoter

    Depositor

    Claudia Vickers

    Article

    Peng et al Metab Eng. 2017 Jan;39:209-219. doi: 10.

    Insert

    TEF1 promoter-yEGFP-ERG9 ER signal peptide
    

    Expression

    Yeast

    Promoter

    TEF1

    Available Since

    Dec. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTCW022

    Plasmid

    #83532

    Purpose

    pheromone mediated expression of UBiC, ARO4, and TKL1

    Depositor

    Claudia Vickers

    Article

    Williams et al Metab Eng. 2015 May;29:124-134. doi: 10.

    Insert

    pFUS1J2-UBiC-CYC1t-pFUS1J2-ARO4*-CYC1t-pFUS1J2-TKL1-CYC1t
    

    Expression

    Yeast

    Promoter

    pFUS1J2

    Available Since

    Dec. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTCW015

    Plasmid

    #83530

    Purpose

    UBiC and feedback resistant ARO4 expression from pheromone inducible FUS1J2 promoter

    Depositor

    Claudia Vickers

    Article

    Williams et al Metab Eng. 2015 May;29:124-134. doi: 10.

    Insert

    pFUS1J2-UBiC-CYC1t-pFUS1J2-ARO4*-CYC1t
    

    Expression

    Yeast

    Promoter

    pFUS1J2

    Available Since

    Dec. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTCW018

    Plasmid

    #83531

    Purpose

    Aromatic amino acid inducible expression of alpha pheromone, and pheromone inducible exression of an ARO7 RNA interference hairpin

    Depositor

    Claudia Vickers

    Article

    Williams et al Metab Eng. 2015 May;29:124-134. doi: 10.

    Insert

    pARO9-mf_2-pFUS1J2-ARO7i-CYC1t
    

    Expression

    Yeast

    Promoter

    pARO9/pFUS1J2

    Available Since

    Oct. 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCas59

    Plasmid

    #82396

    Purpose

    sgRNA targeting YFP +787 from TSS; non-template strand; expressed from aTc inducible system in pEZ03 in the NS1 locus with kanamycin resistance

    Depositor

    Brian Pfleger

    Article

    Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

    Insert

    gRNA
    

    Promoter

    PEZ3

    Available Since

    Sept. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCas45

    Plasmid

    #82390

    Purpose

    sgRNA targeting YFP with additional bases 5' end like pCas34, but from pJ23119 in the NS1 locus with kanamycin resistance

    Depositor

    Brian Pfleger

    Article

    Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

    Insert

    gRNA
    

    Promoter

    PJ23119

    Available Since

    Sept. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCas57

    Plasmid

    #82395

    Purpose

    sgRNA targeting YFP +111 from TSS; template strand; expressed from aTc inducible system in pEZ03 in the NS1 locus with kanamycin resistance

    Depositor

    Brian Pfleger

    Article

    Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

    Insert

    gRNA
    

    Promoter

    PEZ3

    Available Since

    Sept. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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