We narrowed to 5,482 results for: pcas

1. pCAGGS EFF-1::V5

   Plasmid

   #133584

   Purpose

   Expresses EFF-1 A full length protein in mammalian cells

   Depositor

   Benjamin Podbilewicz

   Article

   Avinoam et al Science. 2011 Apr 29;332(6029):589-92. d

   Insert

   EFF-1::V5
   

   Use

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAG-B2UP49_GFP

   Plasmid

   #103071

   Purpose

   Cas9 coding gene template with optimized sequence for human codon usage, also expresses EGFP

   Depositor

   Duhee Bang

   Article

   Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55

   Insert

   B2UP49(Cas9 coding gene from Akkermansia muciniphila (strain ATCC BAA-835))
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   human codon-optimized

   Promoter

   CMV

   Available since

   April 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAFNF-Rac1

   Plasmid

   #163607

   Purpose

   Expresses Rac1 Flp-dependently in mammalian cells

   Depositor

   Takeshi Imai

   Article

   Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

   Insert

   Rac1 (Rac1 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAD108_Arg273His_pCMV6Entry-hIRG1

   Plasmid

   #124878

   Purpose

   Expresses hCAD Arg273His mutant in mammalian cells

   Depositor

   Konrad Buessow

   Article

   Chen et al Proc Natl Acad Sci U S A. 2019 Sep 23. p

   Insert

   cis-aconitate decarboxylase (ACOD1 Human)
   

   Use

   Tags

   myc and FLAG tags

   Expression

   Mammalian

   Mutation

   mutated Arg273 to His

   Promoter

   CMV

   Available since

   Sept. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. Topo_SpCas9.tracr_LbCas12a.DR

   Plasmid

   #155049

   Purpose

   TOPO vector for the cloning of _the SpCas9 tracrRNA - LbCas12a Direct Repeat (DR) fragment into the pLCHKO hgRNA vector

   Depositor

   Benjamin Blencowe, Jason Moffat

   Article

   Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

   Insert

   SpCas9 tracrRNA and LbCas12a Direct Repeat (DR)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAGGS-S-P1-FLAG

   Plasmid

   #177095

   Purpose

   P1 variant S-protein with c-terminal FLAG tag

   Depositor

   Daniel Conway

   Insert

   S-protein P1 (S SARS-CoV-2)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 18, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pCAG-hHDAC4

   Plasmid

   #21096

   Depositor

   Connie Cepko

   Article

   Chen et al Science. 2009 Jan 9. 323(5911):256-9.

   Insert

   Histone Deacetylase 4 (HDAC4 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   shRNA target sequence was mutated (mutated nucleo…

   Promoter

   CAG

   Available since

   May 5, 2009

   Availability

   Academic Institutions and Nonprofits only

8. B-SpCas9-HF1

   Plasmid

   #126762

   Purpose

   Expression plasmid for human codon-opt. increased fidelity Blackjack-SpCas9-HF1 (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than SpCas9HF1.

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

   Insert

   B-SpCas9-HF1
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   N497A; R661A; Q695A; Q926A; amino acids 1005-1013…

   Promoter

   CBh

   Available since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAG-ERas-IH

   Plasmid

   #15688

   Depositor

   Shinya Yamanaka

   Article

   Takahashi et al Nature. 2003 May 29. 423(6939):541-5.

   Insert

   ERas (Eras Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 8, 2008

   Availability

   Academic Institutions and Nonprofits only

10. pCAG-nirButterfly

    Plasmid

    #136590

    Purpose

    Expression of a genetically-encoded voltage indicator nirButterfly

    Depositor

    Vladislav Verkhusha

    Article

    Monakhov et al ACS Chem Neurosci. 2020 Nov 4;11(21):352

    Insert

    nirButterfly
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCA1.332 pDUOTK1-L1

    Plasmid

    #162351

    Purpose

    Level 0 terminator acceptor containing dual fluorophores for testing transcription terminator efficiency

    Depositor

    Alistair McCormick

    Article

    Gale et al Front Microbiol. 2021 Jan 15;11:624011.

    Inserts

    eYFP
    LacZ alpha
    mTagBFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAG-p21_T145A

    Plasmid

    #40203

    Depositor

    Philipp Mergenthaler

    Article

    Mergenthaler et al J Cereb Blood Flow Metab. 2013 Mar;33(3)

    Insert

    p21 (CDKN1A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    T145A

    Promoter

    CAG

    Available since

    March 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. KA2706_eSpCas9-PGKPacdtkCh

    Plasmid

    #124204

    Purpose

    Expression of eSpCas9(1.1) and sgRNA

    Depositor

    Hans Schöler

    Article

    Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAGGS-Hoxc4

    Plasmid

    #45550

    Depositor

    Jeremy Dasen

    Article

    Lacombe et al PLoS Genet. 2013 Jan;9(1):e1003184. doi:

    Insert

    Hoxc4 (Hoxc4 Mouse)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    CMV / b-actin

    Available since

    Aug. 5, 2013

    Availability

    Academic Institutions and Nonprofits only

15. pCAG-p21_T145D

    Plasmid

    #40204

    Depositor

    Philipp Mergenthaler

    Article

    Mergenthaler et al J Cereb Blood Flow Metab. 2013 Mar;33(3)

    Insert

    p21 (CDKN1A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    T145D

    Promoter

    CAG

    Available since

    March 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAMTA2-donor

    Plasmid

    #132501

    Purpose

    CRISPR donor plasmid to create GFP fusion proteins

    Depositor

    Kevin White

    Insert

    CAMTA2 (CAMTA2 Human)
    

    Use

    CRISPR

    Tags

    GFP

    Expression

    Mutation

    none

    Promoter

    Available since

    March 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCAGGS_m-AKAR5’

    Plasmid

    #114140

    Purpose

    Mammalian expression of the AKAR5’ sensor C-terminally fused to the C-terminus of KRas (membrane targeting) for detecting PKA activity using two-photon fluorescence lifetime imaging microscopy (2pFLIM

    Depositor

    Haining Zhong

    Article

    Tillo et al Cell Rep. 2017 Apr 18;19(3):617-629. doi

    Insert

    AKAR5' C-terminally tagged by KRas C tail
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAH-MIBP16

    Plasmid

    #51870

    Purpose

    Expresses C-terminal HA-tagged full length rat MIBP1 (cloned into pCAGGS)

    Depositor

    Kenshi Hayashi, Tomoko Tahira

    Article

    Iwashita et al J Biol Chem. 2012 Mar 23;287(13):9887-90

    Insert

    MIBP1 (Hivep2 Rat)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 13, 2014

    Availability

    Academic Institutions and Nonprofits only

19. pCAD96_hIRG1_4-461_pvp008

    Plasmid

    #124874

    Purpose

    Expresses hCAD Asn152Ser mutant in E.coli

    Depositor

    Konrad Buessow

    Article

    Chen et al Proc Natl Acad Sci U S A. 2019 Sep 23. p

    Insert

    cis-aconitate decarboxylase (ACOD1 Human)
    

    Use

    Tags

    StrepTag and TEV site

    Expression

    Bacterial

    Mutation

    mutated Asn152 to Ser, deleted amino acids 1-3 an…

    Promoter

    T7

    Available since

    Oct. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. Topo_SpCas9.tracr_AsCas12a.DR

    Plasmid

    #155050

    Purpose

    TOPO vector for the cloning of _the SpCas9 tracrRNA - AsCas12a Direct Repeat (DR) fragment into the pLCHKO hgRNA vector

    Depositor

    Benjamin Blencowe, Jason Moffat

    Article

    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

    Insert

    SpCas9 tracrRNA and AsCas12a Direct Repeat (DR)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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