We narrowed to 18,865 results for: IRE;

1. pEF-1α_ZF9LOV[C1]-VP64 (pLZA125)

   Plasmid

   #203910

   Purpose

   Encodes engineered opto-ZF9 with AsLOV2(408-543) inserted in C1 position in mammalian cells

   Depositor

   Jared Toettcher

   Article

   Zhu et al Nat Commun. 2023 Jun 2;14(1):3185. doi:

   Insert

   ZF9LOV[C1]-VP64
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1α

   Available since

   July 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGK415-­luxTA2-­4F

   Plasmid

   #165971

   Purpose

   Expresses luxTA(2-­4F) mutant

   Depositor

   Jun Ishii

   Article

   Tominaga et al Nat Commun. 2021 Mar 23;12(1):1846. doi:

   Insert

   luxR
   

   Use

   Synthetic Biology

   Tags

   Nuclear localization signal and three copies of V…

   Expression

   Yeast

   Mutation

   S116Y, W201R, codon-optimized for expression in y…

   Promoter

   pPGK1 mutant

   Available since

   March 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGEX-2T FRB S2035I

   Plasmid

   #26608

   Depositor

   Jie Chen

   Article

   Vilella-Bach et al J Biol Chem. 1999 Feb 12. 274(7):4266-72

   Insert

   mTOR (MTOR Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   FRB domain only (aa 2015-2114); S2035I

   Promoter

   Available since

   Oct. 28, 2010

   Availability

   Academic Institutions and Nonprofits only

4. pcDNA3.1(+)-Rab10-FLAG

   Plasmid

   #135559

   Purpose

   Express mouse Rab10 in mammalian cells, FLAG at N terminal

   Depositor

   Jingshi Shen

   Article

   Gulbranson et al Proc Natl Acad Sci U S A. 2017 Sep 26;11

   Insert

   Rab10 (Rab10 Mouse)
   

   Use

   Tags

   Flag

   Expression

   Insect

   Mutation

   Promoter

   T7 promoter

   Available since

   Feb. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA-ELYS-emerin-EGFP-polyA

   Plasmid

   #59745

   Purpose

   Express ELYS-emerin-EGFP fusion protein in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

   Depositor

   Yi Zhang

   Article

   Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

   Insert

   ELYS-emerin-EGFP
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and T7

   Available since

   Sept. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEVOL-MmAcKRS1-PylTUUA

   Plasmid

   #182537

   Purpose

   N‐acetyl lysyl‐tRNA synthetase with cognate tRNA for genetic code expansion with ochre suppression

   Depositor

   Wenshe Liu

   Article

   Wu et al Chembiochem. 2012 Jul 9;13(10):1405-8. d

   Insert

   PylTUUA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   ProK

   Available since

   April 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Syn:eGFP-CaM-uTEV1Δ(220-242)

   Plasmid

   #135463

   Purpose

   Neuronal expression of uFLARE protease component

   Depositor

   Alice Ting

   Article

   Sanchez et al Nat Methods. 2019 Dec 9. pii: 10.1038/s4

   Insert

   eGFP-CaM-uTEV1Δ
   

   Use

   AAV

   Tags

   eGFP, V5

   Expression

   Mammalian

   Mutation

   S219V mutation improves stability and S153N impro…

   Promoter

   Synapsin

   Available since

   Jan. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. Cdk6

   Plasmid

   #1866

   Depositor

   Sander van den Heuvel

   Article

   van den Heuvel et al Science 1993 Dec 24;262(5142):2050-4.

   Insert

   Cdk6 (CDK6 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   D224Y

   Promoter

   Available since

   Feb. 14, 2006

   Availability

   Academic Institutions and Nonprofits only

9. pDB036a

   Plasmid

   #99215

   Purpose

   Accessory plasmid for PACE of MjTyrRS variants; negative selection

   Depositor

   David Liu

   Article

   Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038

   Insert

   Ppsp [SD8] gIII; PproK tyrT(Opt,CUA); PproD [SD4] T7RNAP(S12*,S203*)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   amber codons at positions 12 and 203 of T7 RNA po…

   Promoter

   Ppsp, PproK and PproD

   Available since

   Oct. 16, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET14b PHAS-I F113A

    Plasmid

    #15680

    Depositor

    John Lawrence

    Article

    Choi et al J Biol Chem. 2003 May 30. 278(22):19667-

    Insert

    PHAS-I F113A (Eif4ebp1 Rat)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    F113A mutation.

    Promoter

    Available since

    Sept. 24, 2007

    Availability

    Academic Institutions and Nonprofits only

11. mEmerald-MCM6-N-22

    Plasmid

    #54172

    Purpose

    Localization: Nucleus, Excitation: 487, Emission: 509

    Depositor

    Michael Davidson

    Article

    Kuipers et al J Cell Biol. 2011 Jan 10;192(1):29-41. d

    Insert

    MCM6
    

    Use

    Tags

    mEmerald

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 16, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

12. pAAV.CAG-FLEX.iGABASnFR.F102G

    Plasmid

    #112167

    Purpose

    GABA Sensor

    Depositor

    Loren Looger

    Article

    Marvin et al Nat Methods. 2019 Aug;16(8):763-770. doi

    Insert

    iGABASnFR.F102G
    

    Use

    AAV

    Tags

    Expression

    Mutation

    F102G

    Promoter

    CAG

    Available since

    June 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1-GFP-2AFP-KDEL

    Plasmid

    #154349

    Purpose

    Mammalian expression of 2AFP

    Depositor

    Wanzhong He

    Article

    Jiang et al Nat Methods. 2020 Sep;17(9):937-946. doi

    Insert

    2AFP
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pSicoR (Puro) p65 shRNA2

    Plasmid

    #22508

    Depositor

    Tyler Jacks

    Article

    Meylan et al Nature. 2009 Nov 5;462(7269):104-7. doi:

    Insert

    p65 shRNA2 (Rela Mouse)
    

    Use

    Cre/Lox, Lentiviral, and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 1, 2010

    Availability

    Academic Institutions and Nonprofits only

15. pmPB3-VSV

    Plasmid

    #68039

    Purpose

    Expresses mouse Plexin-B3 with N-terminal VSV tag in mammalian cells

    Depositor

    Roland Friedel

    Article

    Maier et al Mol Cell Neurosci. 2011 Feb;46(2):419-31

    Insert

    Plexin-B3 (Plxnb3 Mouse)
    

    Use

    Tags

    VSV

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGEX-4T-3-SH3BP1-CTD

    Plasmid

    #136649

    Purpose

    Expression of SH3BP1-CTD-GST fusion protein in E. coli

    Depositor

    Maria Balda, Karl Matter

    Article

    Elbediwy et al J Cell Biol. 2012 Aug 20;198(4):677-93.

    Insert

    SH3BP1-CTD (SH3BP1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Contains only C-terminal domain

    Promoter

    Available since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pGEX-4T-3-SH3BP1-NTD

    Plasmid

    #136648

    Purpose

    Expression of SH3BP1-NTD-GST fusion protein in E. coli

    Depositor

    Maria Balda, Karl Matter

    Article

    Elbediwy et al J Cell Biol. 2012 Aug 20;198(4):677-93.

    Insert

    SH3BP1-NTD (SH3BP1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Contains only N-terminal domain

    Promoter

    Available since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pMito.iGABASnFR.R205A

    Plasmid

    #112178

    Purpose

    GABA Sensor

    Depositor

    Loren Looger

    Article

    Marvin et al Nat Methods. 2019 Aug;16(8):763-770. doi

    Insert

    iGABASnFR.R205A
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    R205A (no GABA binding)

    Promoter

    Available since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLNHA-C1-HsUPF2-siRNAres_I

    Plasmid

    #146567

    Purpose

    Mammalian Expression of HsUPF2-siRNAres

    Depositor

    Elisa Izaurralde

    Article

    Kashima et al Genes Dev. 2010 Nov 1. 24(21):2440-50.

    Insert

    HsUPF2-siRNAres (UPF2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET14b_tev_PSGU

    Plasmid

    #59738

    Purpose

    Expresses a.a. 417-887 of human ZO-1

    Depositor

    Alan Fanning

    Article

    Nomme et al J Biol Chem. 2011 Dec 16;286(50):43352-6

    Insert

    zonula occludens-1 (TJP1 Human)
    

    Use

    Tags

    HIS tag and thrombin clvg

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 5, 2014

    Availability

    Academic Institutions and Nonprofits only