We narrowed to 67,891 results for: TOR

1. pAAV-hSyn-ASAP3Kv-WPRE

   Plasmid

   #132332

   Purpose

   expresses ASAP3Kv(soma-located version of ASAP3, ASAP-family genetically encoded voltage indicator) in mammalian cells, gene delivered by pAAV-hSyn vector

   Depositor

   Michael Lin

   Article

   Villette et al Cell. 2019 Dec 12;179(7):1590-1608.e23.

   Insert

   ASAP3Kv
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   hSynapsin

   Available Since

   Dec. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pPV-TetO-SpCas9-GR-iC-ERiH (CRONUS-Hygro)

   Plasmid

   #100597

   Purpose

   piggyBac vector expressing Dual-regulated Cas9 (Hygro resistance)

   Depositor

   Akitsu Hotta

   Article

   Ishida et al Sci Rep. 2018 Jan 10;8(1):310. doi: 10.1

   Inserts

   CRISPR Cas9 fused with human Glucocorticoid Receptor
   mCherry
   rtTA-M2
   

   Use

   Piggybac vector

   Expression

   Mammalian

   Mutation

   Codon-optimized for human codon usage

   Promoter

   Dox-inducible TetO promoter and Human EEF1A1 prom…

   Available Since

   April 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. AAV pCAG-FLEX2-tTA2-WPRE-bGHpA

   Plasmid

   #65458

   Purpose

   Can be used to generate AAV virus that will express the tetracycline transactivator tTA2 from the CAG promoter in a Cre-dependent manner

   Depositor

   Hongkui Zeng

   Article

   Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

   Insert

   tTA2
   

   Use

   AAV

   Promoter

   CAG

   Available Since

   July 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. dCas9-GCN5 FL

   Plasmid

   #179552

   Purpose

   encodes S. pyogenes dCas9 with c-terminal fusion of full length human GCN5 driven by EF-1alpha promoter

   Depositor

   Isaac Hilton

   Article

   Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78

   Insert

   S. pyogenes dCas9 with c-terminal fusion of full length human GCN5 (KAT2A Human)
   

   Use

   Lentiviral

   Tags

   Flag Tag

   Expression

   Mammalian

   Mutation

   D10A; H840A in S.pyogenes Cas9

   Promoter

   EF-1alpha

   Available Since

   Feb. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. Lenti-EFS-NTID194-GSQ-PMLivaWT-P2A-BLAST

   Plasmid

   #208038

   Purpose

   Enables constitutive expression of N-terminal Split-TurboID-fused PML (isoform IVa; WT); to perform SUMO-ID; selection with blasticidin

   Depositor

   James Sutherland, Rosa Barrio

   Article

   Barroso-Gomila et al Nat Commun. 2021 Nov 18;12(1):6671. doi:

   Insert

   PML (isoform IVa) (PML Human)
   

   Use

   Lentiviral

   Tags

   FLAG, N-terminal Split-TurboID

   Promoter

   EFS

   Available Since

   Nov. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHR-SIN-BX SpyCatcher003-(short)mCD80

   Plasmid

   #210567

   Purpose

   Expresses Spycatcher003 on the cell surface using mouse CD80 (short) hinge, transmembrane, and cytoplasmic domain

   Depositor

   Omer Dushek

   Article

   Patel et al EMBO J. 2024 Jan;43(1):132-150. doi: 10.

   Insert

   Spycatcher003 fused to mouse CD80 (short hinge + transmembrane + cytoplasmic domain)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   SFFV

   Available Since

   Dec. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-EF1a-DIO-ASAP3-WPRE

   Plasmid

   #132318

   Purpose

   expresses ASAP3(ASAP-family genetically encoded voltage indicator) in cre recombinase expressing mouse/cell, gene delivered by pAAV-EF1a vector

   Depositor

   Michael Lin

   Article

   Villette et al Cell. 2019 Dec 12;179(7):1590-1608.e23.

   Insert

   ASAP3
   

   Use

   AAV and Cre/Lox

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Dec. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSH1/M-FGFR1-Fv-Fvls-E

   Plasmid

   #15285

   Depositor

   David Spencer

   Article

   Freeman et al Cancer Res. 2003 Oct 1. 63(19):6237-43.

   Insert

   FGFR1 kinase, FKBP12v36 (Fgfr1 Mouse)
   

   Tags

   HA epitope and Myristoylation-targeting domain c-…

   Expression

   Mammalian

   Mutation

   FGFR1 cytoplasmic domain FKBP12 F36V

   Available Since

   July 12, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-hSyn-DIO {hCAR}off-{GCaMP6f}on-W3SL

   Plasmid

   #111394

   Purpose

   AAV vector with hSynapsin promoter, Cre-OFF hCAR (for efficient CAV-2 infection), Cre-ON GCaMP6f (ultrasensitive calcium sensor), and W3SL regulatory cassette (for maximize cloning capacity)

   Depositor

   Adam Kepecs

   Article

   Li et al Neuron. 2018 Jun 6;98(5):905-917.e5. doi

   Inserts

   hCAR (CXADR Human)
   GCaMP6f
   

   Use

   AAV

   Tags

   6xHis, Myc, T7, and Xpress

   Expression

   Mammalian

   Promoter

   hSynapsin

   Available Since

   June 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLCA.66/2272

    Plasmid

    #22733

    Depositor

    Mark Magnuson

    Article

    Chen et al Dis Model Mech. 2011 Jul;4(4):537-47. do

    Insert

    Lox66-pU(delta)TK-EM7Neo-Lox2272
    

    Use

    Cre/Lox; Bac recombineering

    Mutation

    This vector was designed for assembling gene targ…

    Available Since

    Dec. 14, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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11. Dyn1(K44A)-mRFP

    Plasmid

    #55795

    Purpose

    Dominant negative Dynamin 1 mutant tagged with mRFP

    Depositor

    Catherine Berlot

    Article

    Hynes et al J Biol Chem. 2004 Oct 15;279(42):44101-1

    Insert

    Dynamin-1(K44A)-mRFP (Dnm1 Discosoma sp., Rat)
    

    Tags

    mRFP

    Expression

    Mammalian

    Mutation

    Dynamin-1(K44A)-mRFP was produced from Dynamin-1(…

    Promoter

    CMV

    Available Since

    Sept. 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. hu-TLR4 399 SNP flag pLenti4/To/v5/DEST

    Plasmid

    #42651

    Purpose

    destination vector for lentivirus-mediated expression of human TLR4 399 SNP in mammalian cells

    Depositor

    Scott Friedman

    Article

    Guo et al Hepatology. 2009 Mar;49(3):960-8. doi: 1

    Insert

    TLR4 399 SNP (TLR4 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    T399I

    Promoter

    CMV/TO

    Available Since

    March 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTagGFP2-N SIN1 deltaCRIM

    Plasmid

    #124921

    Purpose

    For expression of Conserved Region In Middle deletion mutant of SIN1-GFP (delta139-267)

    Depositor

    Jin Chen

    Article

    Hwang et al Cancer Res. 2019 Jul 1;79(13):3178-3184.

    Insert

    MAPKAP1 (MAPKAP1 Human)
    

    Tags

    TagGFP2

    Expression

    Mammalian

    Mutation

    Insert ORF was sirently mutated to be resistent t…

    Available Since

    May 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTagGFP2-N SIN1 deltaPH

    Plasmid

    #124922

    Purpose

    For expression of Pleckstrin Homology domain deletion mutant of SIN1-GFP (delta376-486)

    Depositor

    Jin Chen

    Article

    Hwang et al Cancer Res. 2019 Jul 1;79(13):3178-3184.

    Insert

    MAPKAP1 (MAPKAP1 Human)
    

    Tags

    TagGFP2

    Expression

    Mammalian

    Mutation

    Insert ORF was sirently mutated to be resistent t…

    Available Since

    May 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pINDUCER-21-LCOR-P2A-HOXA9-T2A-HOXA5

    Plasmid

    #97044

    Purpose

    Expresses LCOR, P2A, HOXA9, T2A and HOXA5 in mammalian cells. *Please see notes below on P2A cleaving efficiency.

    Depositor

    George Daley

    Article

    Sugimura et al Nature. 2017 May 17. doi: 10.1038/nature

    Insert

    Ligand Dependent Nuclear Receptor Corepressor, Runt-related transcription factor 1, ETS-related gene
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    Tet on

    Available Since

    Sept. 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pPV-TetO-SpCas9-GR-iC-ERiP (CRONUS-Puro)

    Plasmid

    #100596

    Purpose

    piggyBac vector expressing Dual-regulated Cas9 (Puro resistance)

    Depositor

    Akitsu Hotta

    Article

    Ishida et al Sci Rep. 2018 Jan 10;8(1):310. doi: 10.1

    Inserts

    CRISPR Cas9 fused with human Glucocorticoid Receptor
    mCherry
    rtTA-M2
    

    Use

    Piggybac vector

    Expression

    Mammalian

    Mutation

    Codon-optimized for human codon usage

    Promoter

    Dox-inducible TetO promoter and Human EEF1A1 prom…

    Available Since

    April 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. tetO-NR2F1

    Plasmid

    #170698

    Purpose

    doxycycline-inducible overexpression of NR2F1

    Depositor

    Paul Burridge

    Article

    Romero-Tejeda et al J Mol Cell Cardiol. 2023 Jul 6:S0022-282

    Insert

    nuclear receptor subfamily 2 group F member 1 (NR2F1 Human)
    

    Use

    Lentiviral; Doxycycline inducible

    Expression

    Mammalian

    Mutation

    deletion of 36G- please see depositor comments

    Promoter

    TRE promoter, Tet-ON

    Available Since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. Tet-O-FUW-NR4A3-P2A-mCherry

    Plasmid

    #203863

    Purpose

    Tet-inducible lentiviral plasmid expressing NR4A3-P2A-mCherry, with ORF specific barcode close to 3'LTR site

    Depositor

    Andrea Califano

    Article

    Obradovic et al Cancer Cell. 2023 May 8;41(5):933-949.e1

    Insert

    NR4A3 (NR4A3 Human)
    

    Expression

    Mammalian

    Available Since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. Tet-O-FUW-NR4A1-P2A-mCherry

    Plasmid

    #203859

    Purpose

    Tet-inducible lentiviral plasmid expressing NR4A1-P2A-mCherry, with ORF specific barcode close to 3'LTR site

    Depositor

    Andrea Califano

    Article

    Obradovic et al Cancer Cell. 2023 May 8;41(5):933-949.e1

    Insert

    NR4A1 (NR4A1 Human)
    

    Expression

    Mammalian

    Available Since

    Aug. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. GIV-F1685A FLAG

    Plasmid

    #65948

    Purpose

    Expression GIV/Girdin F1685A mutation in Mammalian cell

    Depositor

    Pradipta Ghosh

    Article

    Lin et al Mol Biol Cell. 2014 Nov 5;25(22):3654-71

    Insert

    GIV/Girdin (CCDC88A Human)
    

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    Phenylalanine 1685 to Alanine

    Promoter

    CMV

    Available Since

    Jan. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

21. B2M Bulldozer (gRNA crB2M_13)

    Plasmid

    #84381

    Purpose

    This plasmid expresses a gRNA targeting the first exon of human B2M. Most efficient gRNA targeting B2M, leading to ablation of B2M and MHC class I surface expression in 50% of transfected cells

    Depositor

    Chad Cowan

    Article

    Mandal et al Cell Stem Cell. 2014 Nov 6;15(5):643-52.

    Insert

    B2M Bulldozer crB2M_13 gRNA
    

    Expression

    Mammalian

    Promoter

    hU6 promoter

    Available Since

    July 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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22. pFBOH-MHL.SUZ12

    Plasmid

    #125172

    Purpose

    Expresses human SUZ12 in insect cells, under a TEV-cleavable N-terminal polyhistidine tag.

    Depositor

    Chen Davidovich

    Article

    Zhang et al Nat Struct Mol Biol. 2019 Mar;26(3):237-

    Insert

    SUZ12 (SUZ12 Human)
    

    Tags

    His-TEV

    Expression

    Insect

    Promoter

    Polyhedrin

    Available Since

    May 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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23. pUAS-ERK-YFP

    Plasmid

    #42076

    Depositor

    Norbert Perrimon

    Article

    Friedman et al Nature. 2006 Nov 9;444(7116):230-4. Epub

    Insert

    Drosophila ERK ortholog (rl Fly)
    

    Tags

    YFP

    Expression

    Insect

    Promoter

    Hsp70

    Available Since

    March 6, 2013

    Availability

    Academic Institutions and Nonprofits only

24. pSH1/M-FGFR2-Fv-Fvls-E

    Plasmid

    #15286

    Depositor

    David Spencer

    Article

    Freeman et al Cancer Res. 2003 Oct 1. 63(19):6237-43.

    Insert

    FGFR2 kinase, FKBP12v36 (Fgfr2 Mouse)
    

    Tags

    Myristoylation-targeting domain c-Src (14aa) and …

    Expression

    Mammalian

    Mutation

    FGFR2 cytoplasmic domain FKBP12 F36V

    Available Since

    July 12, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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25. pBK1303-AAV-EFSNC-dCjCas9-MECP2(204-310)

    Plasmid

    #223148

    Purpose

    Expression of truncated MECP2 with dCjCas9 and empty gRNA scaffold

    Depositor

    Boris Kantor

    Article

    Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

    Insert

    MECP2 (MECP2 Human)
    

    Use

    AAV and CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Encodes aa 204-310

    Promoter

    EF1a

    Available Since

    Aug. 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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26. hu-TLR4 299 SNP flag pLenti 4/to/v5 pDEST

    Plasmid

    #42650

    Purpose

    destination vector for lentivirus-mediated expression of human TLR4 299 SNP in mammalian cells

    Depositor

    Scott Friedman

    Article

    Guo et al Hepatology. 2009 Mar;49(3):960-8. doi: 1

    Insert

    TLR4 299 SNP (TLR4 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    D299G

    Promoter

    CMV/TO

    Available Since

    March 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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27. pBS L30 Flag-Apex-Rab7A

    Plasmid

    #135652

    Purpose

    Rab7A fused to APEX2 and a Flag tag in N-ter under control of a weak L30 promoter.

    Depositor

    Raphael Gaudin

    Article

    Deffieu et al Sci Adv. 2021 Jan 8;7(2). pii: 7/2/eaba7

    Insert

    Rab7A (RAB7A Human)
    

    Tags

    Apex2 and FLag

    Expression

    Mammalian

    Promoter

    L30

    Available Since

    March 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pSH1/M-FGFR3-Fv-Fvls-E

    Plasmid

    #15287

    Depositor

    David Spencer

    Article

    Freeman et al Cancer Res. 2003 Oct 1. 63(19):6237-43.

    Insert

    FGFR3 kinase, FKBP12v36 (Fgfr3 Mouse)
    

    Tags

    Myristoylation-targeting domain c-Src (14aa) and …

    Expression

    Mammalian

    Mutation

    FGFR3 cytoplasmic domain only FKBP12 F36V

    Available Since

    July 12, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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29. mHDAC7-Spliced (1-938) pEF6-V5 (MJS_014) [Edit]

    Plasmid

    #73267

    Purpose

    Expression of mouse HDAC7-S (1-938) in mammalian cells (V5 tag)

    Depositor

    Matthew Sweet

    Article

    Shakespear et al J Biol Chem. 2013 Aug 30;288(35):25362-7

    Insert

    HDAC7 (Hdac7 Mouse)
    

    Tags

    His/V5

    Expression

    Mammalian

    Available Since

    Feb. 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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30. mHDAC7-Spliced (1-938) pEF6-Flag (MJS_015)

    Plasmid

    #73268

    Purpose

    [Edit] Expression of mouse HDAC7-S (1-938) in mammalian cells (Flag tag)

    Depositor

    Matthew Sweet

    Article

    Shakespear et al J Biol Chem. 2013 Aug 30;288(35):25362-7

    Insert

    HDAC7 (Hdac7 Mouse)
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    Feb. 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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