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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 13,590 results for: sequence

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Showing: 11461 - 11490 of 13590 results

  3. A3Ai E72A-Cas9n-UGI-NLS

    Plasmid
    #109430
    Purpose
    Expresses catalytically inactive human APOBEC3A with an L1 intron fused to Cas9n, Uracil DNA Glycosylase Inhibitor, with a nuclear localization signal.
    Depositor
    Reuben Harris
    Article
    St Martin et al Nucleic Acids Res. 2018 May 9. pii: 4994
    Insert
    Apolipoprotein B mRNA Editing Enzyme, Catalytic Polypeptide-Like 3A E72A Catalytic Mutant (APOBEC3A Human)
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    Insertion of an L1 intron into the coding sequenc…
    Promoter
    CMV
    Available Since
    July 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    109430-plasmid-map-sequence-id-212945

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  7. pENT-L1-Kozak-EGFP-GPI-stop-L2

    Plasmid
    #186355
    Purpose
    Entry clone with ORF encoding plasma membrane-targeted EGFP-GPI fusion protein flanked by Gateway recombination sequences
    Depositor
    Patrick Lemaire
    Article
    Roure et al PLoS One. 2007. 2(9):e916.
    Insert
    signal peptide of human CD59, eGFP, aa 67-102 of human CD59 which contains the GPI attachment site (CD59 Human, Synthetic)
    Use
    Expression of a fluorescent membrane marker
    Tags
    EGFP
    Available Since
    Sept. 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    186355-plasmid-map-sequence-id-373423

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  13. pTUU

    Plasmid
    #159749
    Purpose
    Ubiquitin promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter). Selection of transgenic seeds by mTomato fluoresce.
    Depositor
    Wolfgang Lukowitz
    Article
    Angulo et al bioRxiv
    Insert
    gRNA scaffold
    Use
    CRISPR
    Expression
    Plant
    Available Since
    Oct. 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    159749-plasmid-map-sequence-id-313818

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  23. Lyn-FLINC-EKAR1

    Plasmid
    #87707
    Purpose
    Fluorescent biosensor for super-resolution imaging of ERK activity; targeted to plasma membrane.
    Depositor
    Jin Zhang
    Article
    Mo et al Nat Methods. 2017 Apr;14(4):427-434. doi
    Insert
    Lyn-FLINC-EKAR1
    Tags
    N-terminal targeting sequence from Lyn kinase
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Feb. 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    87707-plasmid-map-sequence-id-171091

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  24. PKAsub-TagRFPT-CAAX

    Plasmid
    #87712
    Purpose
    Encodes C-terminal (substrate) fragment of bimolecular super-resolution PKA activity reporter (bimFLINC-AKAR); targeted to plasma membrane; use in conjunction with Dronpa-FHA1 (Addgene #87711).
    Depositor
    Jin Zhang
    Article
    Mo et al Nat Methods. 2017 Apr;14(4):427-434. doi
    Insert
    PKAsub-TagRFPT-CAAX
    Tags
    6xHis, C-terminal targeting sequence from Kras, T…
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Feb. 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    87712-plasmid-map-sequence-id-176157

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  29. Exp-Rosa-EF1a-tTA-mCh-Rs1-TetO(sh)-2

    Plasmid
    #24419
    Purpose
    Human 5HT4B receptor with D100A mutation, generating the RASSL Rs1. Expresses mCherry and Rs1 via P2A ribosomal skip sequence. Expresses tTA under EF1α promoter. Rosa26 homology arms for integration.
    Depositor
    Edward Hsiao
    Article
    Hsiao et al Stem Cell Res Ther. 2011 Mar 4. 2(2):11.
    Inserts
    RS1 (HTR4 Human)
    tTA
    Use
    Mouse Targeting
    Tags
    FLAG and mCherry-P2A
    Mutation
    D100A
    Promoter
    EF1α and TRE, miniCMV
    Available Since
    March 14, 2011
    Availability
    Academic Institutions and Nonprofits only
    24419-plasmid-map-sequence-id-11416

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Showing: 11461 - 11490 of 13590 results