165,688 results

1. pLVX-M-Flag-BAP1-C91A

   Plasmid

   #125841

   Purpose

   express mutant BAP1-C91A

   Depositor

   Boyi Gan

   Article

   Zhang et al Nat Cell Biol. 2018 Oct;20(10):1181-1192

   Insert

   BAP1 (BRCA1 associated protein 1) (BAP1 Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Mutation

   C91A

   Available Since

   June 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pmScarlet3-alphaTubulin_C1

   Plasmid

   #189768

   Purpose

   Construct for labelling microtubules in mammalian cells with mScarlet3-alphatubulin

   Depositor

   Dorus Gadella

   Article

   Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

   Insert

   mScarlet3-alphaTubulin
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAG-Roxed-Cre

   Plasmid

   #51273

   Purpose

   Component of the Co-Driver binary site-specific recombinase system, generates functional rox60-71Cre upon removal of an internal STOP cassette by Dre

   Depositor

   Pawel Pelczar

   Article

   Hermann et al Nucleic Acids Res. 2014 Jan 9.

   Insert

   Roxed-Cre
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   June 2, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBbS8a-RFP

   Plasmid

   #35274

   Depositor

   Jay Keasling

   Article

   Lee et al J Biol Eng. 2011 Sep 20;5:12.

   Insert

   RFP
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   BAD

   Available Since

   Feb. 16, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-hSyn-Cepheid1b-ST-WPRE

   Plasmid

   #214967

   Purpose

   Red fluorescent, negative response-polarity voltage indicator under the control of synapsin promoter; soma-targeted; for brighter fluorescence

   Depositor

   Peng Zou

   Article

   Han et al Sci Adv. 2023 Nov 24;9(47):eadi4208. doi

   Insert

   Cepheid1b-ST
   

   Use

   AAV

   Promoter

   human Synapsin

   Available Since

   March 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBABE-hygro p53 DD

   Plasmid

   #9058

   Depositor

   Bob Weinberg

   Article

   Hahn et al Mol Cell Biol 2002 Apr;22(7):2111-23.

   Insert

   p53 DD (Trp53 Mouse)
   

   Use

   Retroviral

   Expression

   Mammalian

   Mutation

   DD. Dominant negative mutant carries deletion of…

   Available Since

   Dec. 7, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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7. nAChR alpha4 WT

   Plasmid

   #24271

   Depositor

   Henry Lester

   Article

   Nashmi et al J Neurosci. 2003 Dec 17. 23(37):11554-67

   Insert

   nAChR alpha4 WT (Chrna4 Mouse)
   

   Expression

   Mammalian

   Available Since

   April 5, 2010

   Availability

   Academic Institutions and Nonprofits only

8. pET28HIS-hAPE1

   Plasmid

   #70757

   Purpose

   bacterial expression of human APE1

   Depositor

   Primo Schaer

   Article

   Schuermann et al Nucleic Acids Res. 2016 Jan 4. pii: gkv1

   Insert

   APEX1 (APEX1 Human)
   

   Tags

   6-HIS

   Expression

   Bacterial

   Promoter

   T7/lac operon

   Available Since

   Jan. 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pT2/LTR7-GFP

   Plasmid

   #62541

   Purpose

   LTR7-driving reporter

   Depositor

   Zsuzsanna Izsvak

   Article

   Wang et al Nature. 2014 Dec 18;516(7531):405-9. doi

   Insert

   LTR7-GFP
   

   Use

   Sleeping beauty transposon

   Expression

   Mammalian

   Promoter

   LTR7

   Available Since

   Jan. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pZac2.1 gfaABC1D-LifeAct-GFP

    Plasmid

    #190199

    Purpose

    Expresses the small actin binding peptide, LifeAct, with a GFP reporter

    Depositor

    Baljit Khakh

    Article

    Soto et al Nature. 2023 Apr;616(7958):764-773. doi:

    Insert

    LifeAct-eGFP
    

    Use

    AAV

    Tags

    GFP

    Expression

    Bacterial and Mammalian

    Promoter

    gfaABC1D

    Available Since

    April 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET14b – E. coli Exonuclease I

    Plasmid

    #104552

    Purpose

    bacterial expression of Exonuclease I

    Depositor

    Charles Bell

    Article

    Korada et al Nucleic Acids Res. 2013 Jun;41(11):5887-

    Insert

    Exonuclease I (sbcB E.coli)
    

    Tags

    6X-His tag followed by thrombin site

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Dec. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-EF1a-double floxed-Gad67-WPRE-HGHpA

    Plasmid

    #184632

    Purpose

    Expresses Gad67, driven by the Ef1a promoter, in a Cre-dependent fashion

    Depositor

    Nicolas Tritsch

    Article

    Melani et al Cell Rep. 2022 Apr 19;39(3):110716. doi:

    Insert

    Gad67 (Gad1 Mouse)
    

    Use

    AAV

    Promoter

    EF1a

    Available Since

    Aug. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. mCherry-Rab11a-7

    Plasmid

    #55124

    Purpose

    Localization: Ras GTPase, Excitation: 587, Emission: 610

    Depositor

    Michael Davidson

    Insert

    Rab11a (RAB11A Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. EFSp-GFP-YAP (5SA)

    Plasmid

    #174170

    Purpose

    Lentiviral vector to constitutively express overactive YAP (5 LATS phosporylation sites mutated to Ala) under control of the EFS promoter.

    Depositor

    Rod Bremner

    Article

    Pearson et al Cancer Cell. 2021 Jul 13. pii: S1535-610

    Insert

    YAP (YAP1 Human)
    

    Use

    Lentiviral

    Tags

    3x FLAG tag

    Expression

    Mammalian

    Mutation

    Five LATS phosphorylation sites (S61, S109, S127,…

    Promoter

    EFS

    Available Since

    Sept. 28, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pLVX-IRES-Hygro_GCaMP6s

    Plasmid

    #164591

    Purpose

    Stable integration and expression of GCaMP6s in mammalian cells

    Depositor

    Joseph Hyser

    Article

    Chang-Graham et al Sci Rep. 2019 Jul 25;9(1):10822. doi: 10

    Insert

    GCaMP6s
    

    Use

    Lentiviral

    Tags

    6xHis

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pT7-VP6SA11

    Plasmid

    #89166

    Purpose

    Express Rotavirus SA11 VP6 in mammalian cells

    Depositor

    Takeshi Kobayashi

    Article

    Kanai et al Proc Natl Acad Sci U S A. 2017 Jan 30. p

    Insert

    Rotavirus SA11 VP6
    

    Expression

    Mammalian

    Promoter

    T7 promotor

    Available Since

    May 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTagRFP657-N1

    Plasmid

    #31959

    Depositor

    Vladislav Verkhusha

    Article

    Morozova et al Biophys J. 2010 Jul 21;99(2):L13-5.

    Insert

    TagRFP657
    

    Expression

    Mammalian

    Available Since

    Sept. 12, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEGFP-C1-NuMA

    Plasmid

    #81029

    Purpose

    Expresses human NuMA with N-terminal EGFP tag in mammalian cells

    Depositor

    Michael Mancini

    Insert

    Homo sapiens nuclear mitotic apparatus protein 1 (NUMA1) (NUMA1 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pKLV2-EF1a-Cas9Bsd-W

    Plasmid

    #68343

    Purpose

    Lentiviral vector expressing Cas9 fused with the Blasticidin resistant gene at the C-terminus

    Depositor

    Kosuke Yusa

    Article

    Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

    Insert

    EF1a-Cas9 cassette, WPRE
    

    Use

    CRISPR and Lentiviral

    Tags

    Cas9 fused with Flag-tag and a NLS at the N termi…

    Promoter

    EF1a

    Available Since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA-ET

    Plasmid

    #72542

    Purpose

    expresses FLAG tagged human BRD4 ET domain (608-699 aa)

    Depositor

    Christopher Vakoc

    Article

    Shen et al Mol Cell. 2015 Nov 24. pii: S1097-2765(1

    Insert

    BRD4 ET (BRD4 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available Since

    Jan. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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21. pEGFP SNAP47

    Plasmid

    #101915

    Purpose

    Expresses SNAP47 in mammalian cells

    Depositor

    Thierry Galli

    Article

    Kuster et al J Biol Chem. 2015 Nov 20;290(47):28056-6

    Insert

    SNAP47 (SNAP47 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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22. pAAV-DIO-Ef1a-TVA-2A-N2cG

    Plasmid

    #172360

    Purpose

    Targeting envA-pseodotyped RVdG vectors for specific retrograde labeling specifically from cre-expressing neurons

    Depositor

    Yoav Ben-Simon, Peter Jonas

    Article

    Sumser et al Elife. 2022 Aug 30;11:e79848. doi: 10.75

    Insert

    TVA + B19-N2cG
    

    Use

    AAV and Cre/Lox

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Dec. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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23. CRISPR-SB

    Plasmid

    #177936

    Purpose

    Expresses SpCas9 and sgRNA. Can be integrated into the genome upon co-delivery of Sleeping Beauty transposase.

    Depositor

    Roland Rad

    Article

    Kaltenbacher et al Nat Protoc. 2022 Apr;17(4):1142-1188. do

    Type

    Empty backbone

    Use

    CRISPR; Transposon

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Nov. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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24. pEGFP rbFOX2

    Plasmid

    #63086

    Purpose

    Mammalian expression of EGFP tagged FOX2

    Depositor

    Nicolas Charlet-Berguerand

    Insert

    RBFOX2 (RBFOX2 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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25. px459V2.0_Conc2

    Plasmid

    #134451

    Purpose

    Empty concatemer vector in which 2 sgRNAs can be inserted; with Cas9 + Puro

    Depositor

    Daniel Stange

    Article

    Gaebler et al J Vis Exp. 2020 Feb 18;(156). doi: 10.37

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Jan. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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26. pRU55

    Plasmid

    #167684

    Purpose

    Final destination vector for single Gateway LR reaction

    Depositor

    Niko Geldner

    Article

    Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

    Insert

    pEC1.2::asCpf1
    

    Use

    CRISPR

    Available Since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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27. FRB-CD28TMD-CTEVp(L190K)-CS(M)-tTA

    Plasmid

    #137830

    Purpose

    MESA split-TEV protease chain with FRB Rapamycin ectodomain, CD28 transmembrane domain, C-terminal L190K mutant TEV protease, M cleavage sequence, and tTA transcription factor

    Depositor

    Joshua Leonard

    Article

    Dolberg et al Nat Chem Biol. 2021 May;17(5):531-539. d

    Insert

    FRB-CD28TMD-CTEVp(L190K)-CS(M)-tTA
    

    Tags

    3x FLAG

    Expression

    Mammalian

    Mutation

    Mutated Leucine 71 to Lysine in CTEVp fragment

    Promoter

    CMV

    Available Since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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28. pPing

    Plasmid

    #47100

    Purpose

    Expresses the Ping open reading frame 1 (ORF1) and transposase from rice to allow mPing movement. The vector contains ORF1, transposase, mPing element and hph for hygromycin selection.

    Depositor

    Wayne Parrott

    Article

    Hancock et al Plant Physiol. 2011 Oct;157(2):552-62. d

    Inserts

    Ping cDNA
    mPing
    hygromycin resistance gene
    

    Use

    Plant expression

    Tags

    GFP

    Expression

    Yeast

    Promoter

    CaMV35s and StUbi3

    Available Since

    Sept. 16, 2013

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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29. MD-2

    Plasmid

    #20864

    Depositor

    Jie-Oh Lee

    Article

    Park et al Nature. 2009 Apr 30;458(7242):1191-5. do

    Insert

    Myeloid differentiation factor 2 (LY96 Human)
    

    Tags

    protein A tag

    Expression

    Insect

    Mutation

    contains amino acids 19–160

    Available Since

    May 20, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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30. pENTR4-HaloTag (w876-1)

    Plasmid

    #29644

    Depositor

    Eric Campeau

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Entry vector

    Tags

    HaloTag

    Available Since

    May 11, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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