We narrowed to 8,759 results for: epor
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Plasmid#198816PurposeIn vivo calcium indicator. Presence of calcium (Ca2+) increases reporter signal intensity. Based on GCaMP7, improved SNR, slow kineticsDepositorInsert15xUAS::GCaMP7s-SL2-mKate2::let-858 3'UTR
ExpressionWormAvailable SinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTS2308-Tier1-pGL3-PPGK-NLuc_TB
Plasmid#169551PurposeTier-1 expression vector encoding PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 that is insulated via a 5' insulator sequence derived from the pGL3 plasmid (pGL3-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA).DepositorInsertpGL3-insulator-modulated PPGK-driven NanoLuc Luciferase - mTagBFP expression cassette
ExpressionMammalianPromoterPPGKAvailable SinceJune 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pmiRGLO Dual-Luciferase-PDAP1-3'UTR-Mut2x
Plasmid#182254PurposeHuman PDAP1 3` UTR region mutated in 2x binding site for miR-150 cloned downstream of luciferase reporter geneDepositorInsert3' UTR region of PDAP1 gene mutated in 2 binding sites for mir-150
UseLuciferaseExpressionMammalianAvailable SinceApril 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pmiRGLO Dual-Luciferase-PDAP1-3'UTR-Mut1x
Plasmid#182253PurposeHuman PDAP1 3` UTR region mutated in 1x binding site for miR-150 cloned downstream of luciferase reporter geneDepositorInsert3' UTR region of PDAP1 gene mutated in 1 binding site for mir-150
UseLuciferaseExpressionMammalianAvailable SinceApril 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pmiRGLO Dual-Luciferase-PDAP1-3'UTR-Mut3x
Plasmid#182255PurposeHuman PDAP1 3` UTR region mutated in 3x binding site for miR-150 cloned downstream of luciferase reporter geneDepositorInsert3' UTR region of PDAP1 gene mutated in 3 binding sites for mir-150
UseLuciferaseExpressionMammalianAvailable SinceApril 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
p406 – TDH3-ER-FlucDM-GFP11-Ura
Plasmid#177731PurposeDM Firefly luciferase reporter fused to a KAR2 signal sequence for ER targeting and HDEL for ER retention with an HA tag and the eleventh β-strand of GFP added to the C-terminus in yeast plasmid withDepositorInsertFlucDM
TagsGFP11, HA, HDEL, and KAR2 Signal SequenceExpressionBacterial and YeastMutationR188Q, R261Q (Destabilized Mutant)Available SinceJan. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
SIN40C.SFFV.mTagBFP2.miR-125b-2
Plasmid#169311PurposeConstitutive overexpression of miR-125b-2 with mTagBFP2 as a reporter fluorescent proteinDepositorAvailable SinceSept. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGG198
Plasmid#165605PurposeReporter plasmid for B1H-dependent expression of the HIS3/GFP operon with two hEGFP protospacers: PS1(upstream of promoter with 'CAGCG' PAM)-lac-PS2(downstream with 'CGGCG' PAM)-HIS3 and GFPDepositorInserthEGFP protospacer ('CGGCG' PAM) downstream of the HIS3/GFP promoter
UseSynthetic BiologyExpressionBacterialMutationSecondary protospacer ('CGGCG' PAM) ins…PromoterlacAvailable SinceJuly 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLV-PGK-dt/CBA-EGFP-NLS
Plasmid#163918PurposeContains bidirectional reporter genes dtomato and nuclear-targeting EGFP.DepositorInsertdtomato and EGFP
UseLentiviralExpressionMammalianMutationAdded nuclear localization signal to EGFPAvailable SinceFeb. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJW1948
Plasmid#154340PurposeEnhancer reporterDepositorInsertpes-10delta::SV40 NLS::mScarlet_I::PEST(dpi)::-tbb-2 3'UTR
ExpressionWormMutationSilent mutations to remove piRNA sitesAvailable SinceAug. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJWK_VD_04
Plasmid#158105PurposeCaV1.3 5'UTR luciferase reporterDepositorInsert5'UTR of CACNA1D (uc003dgu.5)
UseLuciferaseExpressionMammalianPromoterPGKAvailable SinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJWK_VD_03
Plasmid#158104PurposeCaV1.2 5'UTR luciferase reporterDepositorInsert5'UTR of CACNA1C (uc009zdu.1)
UseLuciferaseExpressionMammalianPromoterPGKAvailable SinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEMS2046
Plasmid#79654PurposessAAV genome with Ple257 (PAX6 MiniPromoter) driving an emerald GFP (EmGFP) reporter. Contains WPREDepositorInsertssAAV Ple257-EmGFP WPRE
UseAAVPromoterPAX6 EnhancerAvailable SinceJuly 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
IX702: pMVP (L3-L2) OLLAS-IRES-eGFP + polyA
Plasmid#121748PurposepMVP L3-L2 entry plasmid, contains OLLAS epitope tag + IRES2-eGFP + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term epitope fusion and IRES expression of eGFP reporterDepositorInsertOLLAS epitope tag-IRES2-eGFP + polyA
UseSynthetic Biology; Pmvp gateway entry plasmidAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFL-SV40-TAK1 5'-UTR
Plasmid#115356Purposefirefly luciferase (Fluc) reporter containing the 5’-UTR of TAK1 mRNADepositorAvailable SinceSept. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
PAK4-FL-3'UTR-MT2
Plasmid#113093PurposeLuciferase reporter containing PAK4-FL- 3' UTR with the second predicted MiR-199a-3p binding site mutatedDepositorInsertp21 Activated kinase 4 (PAK4 Human)
UseLuciferaseExpressionMammalianMutationSecond predicted miR-199a-3p binding site mutated…PromoterPGKAvailable SinceAug. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
PAK4-FL-3'UTR-MT-both
Plasmid#113094PurposeLuciferase reporter containing PAK4-FL- 3' UTR with both the predicted MiR-199a-3p binding sites mutatedDepositorInsertp21 Activated kinase 4 (PAK4 Human)
UseLuciferaseExpressionMammalianMutationBoth the predicted miR-199a-3p binding site muta…PromoterPGKAvailable SinceAug. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSi-check2-hRac2 3'UTR
Plasmid#97160PurposeFor dual luciferase report assayDepositorInsertRAC 3'UTR (AKT1 Human)
ExpressionMammalianAvailable SinceOct. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEMS2047
Plasmid#79655PurposessAAV genome with Ple258 (PAX6 MiniPromoter) driving an emerald GFP (EmGFP) reporter. Contains WPREDepositorInsertssAAV Ple258-EmGFP WPRE
UseAAVPromoterPAX6 EnhancerAvailable SinceSept. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
exon K-renilla
Plasmid#81007Purposeluciferase based mini-gene to report splicing at exon 25 in Drosophila paraylticDepositorInsertparalytic (para Fly)
TagsRenilla luciferaseExpressionInsectMutationspanning exon 24 to exon 26, termination codon in…PromoterP-AC5 (Actin 5C)Available SinceAug. 19, 2016AvailabilityAcademic Institutions and Nonprofits only