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Showing: 7721 - 7740 of 9077 results

  1. N-Terminal Split Cas9 D10A Nickase with GyrA intein

    Plasmid
    #58695
    Purpose
    Expresses N-terminus of D10A SpCas9 nickase domain fused to a GyrA intein, flanked by ITRs for AAV packaging. Combine with C-Terminal Split Cas9 Gyra Intein for full length SpCas9 nickase production
    Depositor
    Gang Bao
    Article
    Fine et al Sci Rep. 2015 Jul 1;5:10777. doi: 10.103
    Insert
    D10A Nickase humanized S. pyogenes Cas9 with Gyra Nsplit Intein
    Use
    AAV and CRISPR
    Tags
    GyrA Nsplit Intein, HA Tag, and NLS
    Expression
    Mammalian
    Mutation
    D10A nickase converting mutation to SpCas9
    Promoter
    CBh
    Available Since
    Sept. 25, 2014
    Availability
    Academic Institutions and Nonprofits only
    58695-plasmid-map-sequence-id-90694

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  4. pcDNA5-FRT/TO-mNeonGreen-BRD4-NUT(deltaMIN)

    Plasmid
    #204696
    Purpose
    This plasmid allows for inducible expression of BRD4-NUT(deltaMIN) mutant fusion tagged with mNeonGreen, in mammalian cells.
    Depositor
    Michael Rosen
    Article
    Kosno et al Sci Rep. 2023 Jul 24;13(1):11907. doi: 1
    Insert
    BRD4-NUT(deltaMIN) (BRD4, NUTM1 Human)
    Tags
    mNeonGreen
    Expression
    Mammalian
    Mutation
    deletion of the amino acids 355-505 of NUT
    Promoter
    CMV
    Available Since
    Aug. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    204696-plasmid-map-sequence-id-405172

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  5. pcDNA5-FRT/TO-mNeonGreen-BRD4-NUT(MIN)

    Plasmid
    #204695
    Purpose
    This plasmid allows for inducible expression of BRD4-NUT(MIN) mutant fusion tagged with mNeonGreen, in mammalian cells.
    Depositor
    Michael Rosen
    Article
    Kosno et al Sci Rep. 2023 Jul 24;13(1):11907. doi: 1
    Insert
    BRD4-NUT(MIN) (BRD4, NUTM1 Human)
    Tags
    mNeonGreen
    Expression
    Mammalian
    Mutation
    deletion of majority of NUT, retaining only amino…
    Promoter
    CMV
    Available Since
    Aug. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    204695-plasmid-map-sequence-id-405171

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  19. pDONR223 SARS-CoV-2 NSP6_nostop

    Plasmid
    #149309
    Purpose
    Gateway-compatible Entry vector, with insert of NSP6 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1. Does not contain stop codon.
    Depositor
    Fritz Roth
    Article
    Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4
    Insert
    NSP6 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)
    Use
    Gateway-compatible entry vector
    Mutation
    Many synonymous changes due to codon optimization
    Available Since
    May 6, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    149309-plasmid-map-sequence-id-296438

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Showing: 7721 - 7740 of 9077 results