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pLenti.hUbC.H2B-CeruleanFP-2A-Dendra2FP.W

Plasmid

#126522

Purpose

Lentivector that expresses H2B-Cerulean and Dendra2 fluorescent proteins from an internal hUbC promoter.

Depositor

Rusty Lansford

Article

Huss et al Front Cell Dev Biol. 2019 Mar 28;7:35. d

Insert

H2B-CeruleanFP-2A-Dendra2FP

Use

Lentiviral

Promoter

hUbC

Available Since

May 22, 2019

Availability

Academic Institutions and Nonprofits only

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pGP-CAG-iGABASnFR2n-WPRE-bGH-polyA

Plasmid

#218869

Purpose

Mammalian expression of improved GABA sensor (negative change in fluorescence)

Depositor

GENIE Project

Article

Kolb et al eLife. 2026 Mar 18;14:RP108319. doi: 10.

Insert

iGABASnFR2n

Expression

Mammalian

Mutation

S99A F104H R168P

Promoter

CAG

Available Since

June 5, 2024

Availability

Academic Institutions and Nonprofits only

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pRnc-gGFP161

Plasmid

#189536

Purpose

Expresses E. Coli RNase III under control of pBAD promoter and constitutively expresses gUTR-161-GFP

Depositor

Marc Ostermeier

Article

Hauk et al CRISPR J. 2022 Dec 9. doi: 10.1089/crisp

Inserts

RNase III
gUTR-161-GFP

Expression

Bacterial

Mutation

N/A

Promoter

J23119 and pBAD

Available Since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_TOMM20-STf

Plasmid

#182002

Purpose

CMV overexpression of SNAP-tag fast at the mitochondria surface of mammalian cell lines

Depositor

Kai Johnsson

Article

Frei et al ACS Chem Biol. 2022 Jun 17;17(6):1321-13

Insert

TOMM20-STf

Expression

Mammalian

Mutation

Fast mutation on SNAP-tag E30R

Promoter

CMV

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pDR111_Bgal NLS

Plasmid

#188396

Purpose

Expresses beta-galactosidase via Phyper-spank which is optimized for Bacillus subtilis. There is a secretion peptide (PhoD) and nuclear localization signal (SV40) that is fused to beta-galactosidase.

Depositor

Christopher Contag

Article

Madsen et al Commun Biol. 2022 Aug 30;5(1):888. doi:

Insert

lacZ

Tags

PhoD secretion peptide and SV40 NLS

Expression

Bacterial

Promoter

Phyper spank

Available Since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pPagP

Plasmid

#112125

Purpose

Bacterial expression of outer membrane protein PagP, wild type

Depositor

Karen Fleming

Article

Burgess et al J Biol Chem. 2008 Sep 26;283(39):26748-5

Insert

PagP (pagP E. coli)

Expression

Bacterial

Mutation

N-terminal signal sequence not included and repla…

Promoter

T7

Available Since

Nov. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pOmpW

Plasmid

#112121

Purpose

Bacterial expression of outer membrane protein W (OmpW), wild type

Depositor

Karen Fleming

Article

Burgess et al J Biol Chem. 2008 Sep 26;283(39):26748-5

Insert

OmpW (ompW E. coli)

Expression

Bacterial

Mutation

N-terminal signal sequence not included and repla…

Promoter

T7

Available Since

Nov. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pFlg167

Plasmid

#101019

Purpose

fluorescent, functional, glutamate receptor subunit

Depositor

Thomas Hughes

Article

Sheridan et al Neuroscience. 2006 Aug 25;141(2):837-49.

Insert

GluR 1 (Gria1 Rat)

Expression

Mammalian

Mutation

fluorescent protein inserted into subunit

Promoter

cmv

Available Since

Sept. 28, 2018

Availability

Academic Institutions and Nonprofits only

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mKO-Mito-7

Plasmid

#57848

Purpose

Localization: Mitochondria, Excitation: 548, Emission: 561

Depositor

Michael Davidson

Article

Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

Insert

Mito (COX8A Human)

Tags

mKO

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 24, 2014

Availability

Academic Institutions and Nonprofits only

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mKO-Fibrillarin-7

Plasmid

#57842

Purpose

Localization: Nuclear - Nucleolus, Excitation: 548, Emission: 561

Depositor

Michael Davidson

Article

Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

Insert

Fibrillarin (FBL Human)

Tags

mKO

Expression

Mammalian

Mutation

R36Q, S66F

Promoter

CMV

Available Since

Oct. 24, 2014

Availability

Academic Institutions and Nonprofits only

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mTFP1-VASP-5

Plasmid

#55513

Purpose

Localization: Actin/Focal Adhesions, Excitation: 462, Emission: 492

Depositor

Michael Davidson

Article

Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

Insert

VASP

Tags

mTFP1

Expression

Mammalian

Mutation

A209T in VASP

Promoter

CMV

Available Since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only
mTFP1-ZO1-C-14

Plasmid

#55518

Purpose

Localization: Tight Junctions, Excitation: 462, Emission: 492

Depositor

Michael Davidson

Article

Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

Insert

ZO1 (TJP1 Human)

Tags

mTFP1

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only
mTFP1-H1-10

Plasmid

#55487

Purpose

Localization: Nucleus/Histones, Excitation: 462, Emission: 492

Depositor

Michael Davidson

Article

Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

Insert

H1 (H1f0 Mouse)

Tags

mTFP1

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only
pet28a-ClbB-NRPS-NHis

Plasmid

#49217

Purpose

NRPS module of ClbB containing CAT domains with N-terminal 6His tag

Depositor

Emily Balskus

Article

Brotherton et al J Am Chem Soc. 2013 Mar 6;135(9):3359-62

Insert

ClbB-NRPS

Tags

6x His tag and T7 tag

Expression

Bacterial

Mutation

contains aa4-1080 of reference sequence NP_754362…

Promoter

T7

Available Since

March 26, 2014

Availability

Academic Institutions and Nonprofits only

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pBS-NT-AscI

Plasmid

#15461

Depositor

Peter Mombaerts

Article

Walz et al J Neurobiol. 2006 Jul . 66(8):835-46.

Insert

neurotensin (Nts Mouse)

Mutation

Added AscI site 3 bp 3' of stop codon

Available Since

Aug. 24, 2007

Availability

Academic Institutions and Nonprofits only
pMRS-BG42-GFP

Plasmid

#220202

Purpose

mini-Tn7 GFP expression vector - pBG42 promoter

Depositor

Larry Halverson

Article

Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

Insert

monomeric superfolder green fluorescent protein

Use

CRISPR

Expression

Bacterial

Mutation

monomeric superfolder green fluorescent protein

Promoter

pBG42

Available Since

Sept. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pCLV3Cas-LT1

Plasmid

#210549

Purpose

Binary vector expressing the Lineage Tracing system. Cas9 expression driven by CLV3 promoter. LT1 reporter

Depositor

Ortrun Mittelsten Scheid

Article

Dona et al Plant J. 2023 Jul 5. doi: 10.1111/tpj.16

Insert

CLV3p::spCas9::PEA3At::U6p::AAVS1gRNA::pHTR5::LT1::mCherrySSM

Expression

Plant

Mutation

2bp insertion after AAVS1 target sequence that di…

Available Since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV_tKiMBI-T2A-caMEK

Plasmid

#199578

Purpose

Expresses tKiMBI and caMEK in an AAV vector

Depositor

Michael Lin

Article

Wu et al ACS Cent Sci. 2023 Mar 20;9(4):719-732.

Inserts

ERK tdTomato-Kinase-Modulated Bioluminescent Indicator
constitutively active MEK

Use

AAV

Expression

Mammalian

Promoter

CMV

Available Since

June 23, 2023

Availability

Academic Institutions and Nonprofits only

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pCSIIpuro-4493NES

Plasmid

#175811

Purpose

The lentiviral vector for Booster-PKA, a Red-Shifted genetically encoded FRET biosensor for monitoring PKA activity.

Depositor

Michiyuki Matsuda

Article

Watabe et al ACS Sens. 2020 Feb 26. doi: 10.1021/acss

Insert

Booster-PKA

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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FB005

Plasmid

#119678

Purpose

Coding sequence nptII from E. coli domesticated into pUPD2 with AATG/GCTT barcodes. According to FungalBraid/GoldenBraid modular DNA assembly for A. tumefaciens-mediated genetic transformation

Depositor

Jose Marcos

Article

Hernanz-Koers et al Fungal Genet Biol. 2018 Jul;116:51-61. d

Insert

geneticin resistance marker

Use

Synthetic Biology; Domestication of dna parts for…

Available Since

March 28, 2019

Availability

Academic Institutions and Nonprofits only

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