We narrowed to 15,702 results for: grna

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U6_sgRNA_CAG_ABE7.10_St1Cas9_LMD9

Plasmid

#136660

Purpose

Expresses ABE St1Cas9 LMD9 in mammalian cells along with its U6-driven sgRNA

Depositor

Yannick Doyon

Article

Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:

Inserts

St1Cas9 LMD-9
sgRNA for St1Cas9
ABE7.10

Use

CRISPR and Synthetic Biology

Tags

SV40 NLS and hSt1Cas9

Expression

Mammalian

Promoter

CAG and U6

Available Since

Feb. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-sgRNA(lib)-puro

Plasmid

#119976

Purpose

Lenti sgRNA cloning backbone with CMV-puro resistance marker. Contains BsmBI sites for insertion of spacer sequences.

Depositor

Wensheng Wei

Article

Liu et al Nat Biotechnol. 2018 Nov 5. pii: nbt.428

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

March 11, 2019

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(Empty)-PGKGFP2ABFP-W

Plasmid

#67983

Purpose

Cas9 activity reporter (control) with GFP and BFP

Depositor

Kosuke Yusa

Article

Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

Insert

U6gRNA cassette, PGKGFP2ABFP cassette, WPRE

Use

CRISPR and Lentiviral

Mutation

Deleted BbsI site within WPRE, modified BFP

Available Since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pHLS-EF1a-FRB-SpCas9-A

Plasmid

#138477

Purpose

Expresses FRB fused SpCas9 for NanoMEDIC packaging.

Depositor

Akitsu Hotta

Article

Gee et al Nat Commun. 2020 Mar 13;11(1):1334. doi:

Insert

SpCas9, human codon optimized

Use

CRISPR

Tags

3x HA tag and FRB

Expression

Mammalian

Available Since

March 23, 2020

Availability

Academic Institutions and Nonprofits only

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psgRNA2.02-MS2-ccdB

Plasmid

#99911

Purpose

Basic cloning vector for the expression of MS2 inserted sgRNA

Depositor

Yujie Sun

Article

Shao et al Nucleic Acids Res. 2016 May 19;44(9):e86

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

Oct. 10, 2017

Availability

Academic Institutions and Nonprofits only

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pCK005_U6-Sp-sgRNA_UP-TdTomato_WPRE

Plasmid

#85453

Purpose

Lentiviral CRISPR system backbone bearing BsmBI site for S. pyogenes new guide RNAs and TdTomato.

Depositor

Aviv Regev

Article

Singer et al Cell. 2016 Sep 8;166(6):1500-1511.e9. do

Insert

U6-sgRNA-Backbone-UP-TdTomato

Use

CRISPR and Lentiviral

Tags

NLS

Expression

Mammalian

Mutation

deletion of AA123-365 in tdTomato (please see dep…

Available Since

April 19, 2017

Availability

Academic Institutions and Nonprofits only

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PEmax-mRNA

Plasmid

#204472

Purpose

plasmid for PEmax mRNA IVT

Depositor

Scot Wolfe

Article

Ponnienselvan et al Nucleic Acids Res. 2023 May 29:gkad456.

Insert

Prime editor

Use

CRISPR

Tags

no

Expression

Mammalian

Promoter

T7

Available Since

Aug. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pL-U6-sgRNA-SFFV-Puro-P2A-EGFP

Plasmid

#175037

Purpose

Lentiviral delivery of sgRNA. Expresses PuroR and eGFP from SFFV promoter.

Depositor

Kristian Helin

Article

Muller et al Nat Commun. 2021 May 24;12(1):3034. doi:

Insert

Sp sgRNA scaffold

Use

Lentiviral

Promoter

U6

Available Since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pPN458

Plasmid

#137876

Purpose

PiggyBac vector for expression of 1x gRNA targeting TCF4 for CRISPRa;mRFP-T2A-BlasticidinR

Depositor

Lindy Barrett

Article

Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

Insert

TCF4 (TCF4 Human)

Use

CRISPR

Expression

Mammalian

Available Since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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STAgR_tdTomato

Plasmid

#102993

Purpose

Can be used as backbone for a STAgR reaction

Depositor

Stefan Stricker

Article

Breunig et al PLoS One. 2018 Apr 27;13(4):e0196015. do

Type

Empty backbone

Use

CRISPR; Pcr template for stagr vectors

Promoter

U6

Available Since

March 13, 2019

Availability

Academic Institutions and Nonprofits only

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PX330-TH-sgRNA-1

Plasmid

#247673

Purpose

All-in-one CRISPR construct targeting TH

Depositor

Ellen Sidransky

Article

Hertz et al Mov Disord. 2025 Aug;40(8):1719-1724. do

Insert

TH sgRNA 1 (TH Human)

Use

CRISPR

Expression

Mammalian

Available Since

June 3, 2026

Availability

Academic Institutions and Nonprofits only

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PX330-TH-sgRNA-2

Plasmid

#247674

Purpose

All-in-one CRISPR construct targeting TH

Depositor

Ellen Sidransky

Article

Hertz et al Mov Disord. 2025 Aug;40(8):1719-1724. do

Insert

TH sgRNA 2 (TH Human)

Use

CRISPR

Expression

Mammalian

Available Since

June 3, 2026

Availability

Academic Institutions and Nonprofits only

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pAT15535_hU6_epegRNA_Ef1a-mCh

Plasmid

#251104

Purpose

epegRNA cloning plasmid + mCherry expression

Depositor

Gerald Schwank

Article

Talas et al Sci Transl Med. 2026 May 13;18(849):eaec

Insert

pegRNA cloning vector

Use

CRISPR

Available Since

May 14, 2026

Availability

Academic Institutions and Nonprofits only

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pAT159_AAV_p3_PE7-C_hU6_epegRNA

Plasmid

#251105

Purpose

Encodes the C terminal of the PE7 prime editor in an AAV expression plasmid + pegRNA cloning casette

Depositor

Gerald Schwank

Article

Talas et al Sci Transl Med. 2026 May 13;18(849):eaec

Insert

PE7 C-terminal

Use

AAV and CRISPR

Promoter

p3

Available Since

May 14, 2026

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPRv2_mPTK7-gRNA4

Plasmid

#249199

Purpose

mouse PTK7 CRISPR KO

Depositor

Weihong Tan

Article

Luo et al Science. 2026 Jan;391(6780):eadv6127. do

Insert

PTK7 (Ptk7 Mouse)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

April 15, 2026

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPRv2_hPTK7-gRNA2

Plasmid

#249197

Purpose

human PTK7 CRISPR KO

Depositor

Weihong Tan

Article

Luo et al Science. 2026 Jan;391(6780):eadv6127. do

Insert

PTK7 (PTK7 Human)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

April 15, 2026

Availability

Academic Institutions and Nonprofits only

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sgRNA(nt)-IM83

Plasmid

#251969

Purpose

Control sgRNA with IM83 aptamer for yeast mtDNA editing

Depositor

Alice Ting

Article

Yin et al Proc Natl Acad Sci U S A. 2026 Jan 20;12

Insert

sgRNA(nt)-IM83

Use

CRISPR

Expression

Yeast

Promoter

SNR52

Available Since

March 30, 2026

Availability

Academic Institutions and Nonprofits only

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sgRNA-IM83

Plasmid

#251967

Purpose

sgRNA with IM83 aptamer for yeast mtDNA editing

Depositor

Alice Ting

Article

Yin et al Proc Natl Acad Sci U S A. 2026 Jan 20;12

Insert

sgRNA-IM83

Use

CRISPR

Expression

Yeast

Promoter

SNR52

Available Since

March 18, 2026

Availability

Academic Institutions and Nonprofits only

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sgRNA-msIM83

Plasmid

#251968

Purpose

sgRNA for yeast mtDNA editing, with IM83 aptamer inserted into the major stem-loop of the sgRNA

Depositor

Alice Ting

Article

Yin et al Proc Natl Acad Sci U S A. 2026 Jan 20;12

Insert

sgRNA-msIM83

Use

CRISPR

Expression

Yeast

Promoter

SNR52

Available Since

March 18, 2026

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPRv2_mPTK7-gRNA3

Plasmid

#249198

Purpose

mouse PTK7 CRISPR KO

Depositor

Weihong Tan

Article

Luo et al Science. 2026 Jan;391(6780):eadv6127. do

Insert

PTK7 (Ptk7 Mouse)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

Feb. 17, 2026

Availability

Academic Institutions and Nonprofits only

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