We narrowed to 26,306 results for: Vit

1. Circular 100,50 (RAB7A)

   Plasmid

   #170117

   Purpose

   AAV vector carrying a guide RNA targeting the human RAB7A mRNA

   Depositor

   Prashant Mali

   Article

   Katrekar et al Nat Biotechnol. 2022 Feb 10. pii: 10.103

   Insert

   Circular 100,50 guide RNA
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   Human U6

   Available Since

   Feb. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJT130_GalL_nCDA1-NGBE3

   Plasmid

   #145094

   Purpose

   Expressing base editor nCDA1-NGBE3 in yeast cells

   Depositor

   Ralph Bock

   Article

   Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

   Insert

   nCDA1-NGBE3
   

   Use

   CRISPR

   Expression

   Yeast

   Mutation

   spCas9(D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1…

   Promoter

   GalL

   Available Since

   July 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. SSTR2-DuET

   Plasmid

   #213377

   Purpose

   The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

   Depositor

   Thomas Sakmar

   Article

   Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

   Insert

   SSTR2 (SSTR2 Human)
   

   Tags

   1D4 and FLAG

   Expression

   Mammalian

   Promoter

   CMV, T7

   Available Since

   April 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. Circular 200,100 with interspersed loops (SMAD4)

   Plasmid

   #180191

   Purpose

   AAV vector carrying a guide RNA targeting the human SMAD4 mRNA

   Depositor

   Prashant Mali

   Article

   Katrekar et al Nat Biotechnol. 2022 Feb 10. pii: 10.103

   Insert

   Circular 200,100 guide RNA with interspersed loops
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   Human U6

   Available Since

   April 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV5-rat ERK2-WT

   Plasmid

   #40812

   Depositor

   Natalie Ahn

   Article

   Emrick et al Proc Natl Acad Sci U S A. 2006 Nov 28;10

   Insert

   MAPK1 (Mapk1 Rat)
   

   Tags

   His6

   Expression

   Mammalian

   Mutation

   wild type

   Promoter

   CMV

   Available Since

   Oct. 18, 2012

   Availability

   Academic Institutions and Nonprofits only

6. pAAV.CAG.GCaMP6m.WPRE.SV40

   Plasmid

   #100840

   Purpose

   AAV expression of ultrasensitive protein calcium sensor from CAG promoter

   Depositor

   Douglas Kim, GENIE Project

   Article

   Chen et al Nature. 2013 Jul 18;499(7458):295-300. d

   Has Service

   AAV1

   Insert

   GCaMP6m
   

   Use

   AAV

   Expression

   Mammalian

   Mutation

   GCaMP3-T302L R303P M378G K379S D380Y T381R S383T …

   Promoter

   CAG

   Available Since

   Nov. 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pACT2-CAS9c

   Plasmid

   #51055

   Purpose

   Express Eukaryotic-codon-optimized Cas9c gene in yeast under ADH1 promoter for genome editing. SV40 NLS is fused to the C terminal. GAL4 region from pACT2 is removed.

   Depositor

   Yunde Zhao

   Article

   Gao et al J Integr Plant Biol. 2013 Dec 30. doi: 1

   Insert

   Cas9c
   

   Use

   CRISPR

   Tags

   SV40 NLS

   Expression

   Bacterial and Yeast

   Promoter

   yeast ADH1 promoter

   Available Since

   Feb. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTV-Sox2_5prime-1000-24MS96T-3F_NeoR

   Plasmid

   #174884

   Purpose

   Targeting vector for generating Sox2 STREAMING-tag KI cell

   Depositor

   Hiroshi Ochiai

   Article

   Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

   Insert

   STREAMING-tag with homology arms for Sox2
   

   Use

   Mouse Targeting

   Available Since

   Dec. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-EFS-DIO-GRAB_Ado1.0med

   Plasmid

   #153292

   Purpose

   Express the medium-affinity adenosine sensor GRAB_Ado1.0med in Cre positive cells

   Depositor

   Yulong Li

   Article

   Wu et al Proc Natl Acad Sci U S A. 2023 Apr 4;120

   Insert

   Adenosine sensor GRAB_Ado1.0med (medium affinity)
   

   Use

   AAV

   Promoter

   EFS

   Available Since

   July 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV3Tag8li-hDDX50-HA

    Plasmid

    #165465

    Purpose

    Human DDX50 mammalian expression vector with C-terminal HA tag.

    Depositor

    Martin Dorf

    Article

    Li et al Proc Natl Acad Sci U S A. 2014 May 13;11

    Insert

    DExD-box helicase 50 (DDX50 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV.Syn.Flex.GCaMP6m.WPRE.SV40

    Plasmid

    #100838

    Purpose

    AAV expression of Cre recombinase-activated ultrasensitive protein calcium sensor from Synapsin promoter

    Depositor

    Douglas Kim, GENIE Project

    Article

    Chen et al Nature. 2013 Jul 18;499(7458):295-300. d

    Has Service

    AAV1 and AAV9

    Insert

    GCaMP6m
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    GCaMP3-T302L R303P M378G K379S D380Y T381R S383T …

    Promoter

    Synapsin

    Available Since

    Nov. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. VEGF binding scFv G6

    Plasmid

    #111716

    Purpose

    Yeast Surface Display of G6 as a reference starting point for design

    Depositor

    Sarel Fleishman

    Article

    Warszawski et al PLoS Comput Biol. 2019 Aug 23;15(8):e100

    Insert

    G6
    

    Tags

    cmyc tag

    Expression

    Yeast

    Available Since

    July 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGEMHE:mTREK-1(tandem wt-wt)

    Plasmid

    #224757

    Purpose

    In vitro transcription for Oocyte expression of mTREK-1(tandem wt-wt)

    Depositor

    Dan Minor

    Article

    Bagriantsev et al EMBO J. 2012 Aug 1;31(15):3297-308. doi:

    Insert

    Tandem TREK-1 (wt-wt) (Kcnk2 Mouse)
    

    Use

    In vitro transcription for oocyte expression

    Promoter

    T7

    Available Since

    Sept. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pPBC-LG6s

    Plasmid

    #62809

    Purpose

    pPBC-LG6s expresses PiggyBac inverted terminal repeat-flanked Lck-GCaMP6s under the control of the CAG promoter.

    Depositor

    Karen Wilcox

    Article

    Gee et al Front Mol Neurosci. 2015 Apr 15;8:10. do

    Insert

    ITR-CAG-Lck-GCaMP6s-ITR
    

    Expression

    Bacterial and Mammalian

    Promoter

    CAG

    Available Since

    May 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. Sniper-Cas9

    Plasmid

    #138559

    Purpose

    Expresses human codon-optimized Sniper-SpCas9 and blasticidin resistance: EFS promoter-Sniper-Cas9-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

    Insert

    Sniper-Cas9
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Mutation

    F539S, M763I, K890N

    Promoter

    EFS

    Available Since

    June 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. AAV-FLEX-FLIM-AKART391A

    Plasmid

    #60446

    Purpose

    Expresses the non-phosphorylatable point mutant (T391A) of FLIM-AKAR in a Cre dependent manner in an AAV backbone

    Depositor

    Bernardo Sabatini

    Article

    Chen et al Front Pharmacol. 2014 Apr 2;5:56. doi: 1

    Insert

    FLIM-AKART391A
    

    Use

    AAV and Cre/Lox

    Expression

    Mammalian

    Mutation

    changed Threonine 391 to Ananine

    Promoter

    CAG

    Available Since

    Oct. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHAGE-FLAG-APEX2-LGALS1

    Plasmid

    #175755

    Purpose

    Lentiviral vector expressing N-terminally tagged APEX2-FLAG-LGALS1

    Depositor

    Wade Harper

    Article

    Eapen et al eLife. 2021 Sep 29;10. pii: 72328. doi:

    Insert

    LGALS1 (LGALS1 Human)
    

    Use

    Lentiviral

    Available Since

    Jan. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAG-Bmpr2ΔKinase

    Plasmid

    #163404

    Purpose

    Expresses BMPR-2 lacking the kinase domain in mammalian cells

    Depositor

    Takeshi Imai

    Article

    Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

    Insert

    Bmpr2 (Bmpr2 Mouse)
    

    Expression

    Mammalian

    Mutation

    The kinase domain (202-500 aa) is deleted

    Available Since

    Aug. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV.CAG.Flex.GCaMP6m.WPRE.SV40

    Plasmid

    #100839

    Purpose

    AAV expression of Cre recombinase-activated ultrasensitive protein calcium sensor from CAG promoter

    Depositor

    Douglas Kim, GENIE Project

    Article

    Chen et al Nature. 2013 Jul 18;499(7458):295-300. d

    Has Service

    AAV1, AAV5, and AAV9

    Insert

    GCaMP6m
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    GCaMP3-T302L R303P M378G K379S D380Y T381R S383T …

    Promoter

    CAG

    Available Since

    Nov. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. GIT1A-FLAG

    Plasmid

    #15225

    Depositor

    Rick Horwitz

    Article

    Manabe et al J Cell Sci. 2002 Apr 1. 115(Pt 7):1497-5

    Insert

    G protein coupled receptor kinase-interacting protein 1 (GIT1 Human)
    

    Tags

    flag

    Expression

    Mammalian

    Available Since

    Sept. 13, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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