19,660 results

1. pCold-TF-hRubicon

   Plasmid

   #64147

   Purpose

   For protein expression in E. coli

   Depositor

   Tamotsu Yoshimori

   Article

   Tabata et al Mol Biol Cell. 2010 Dec;21(23):4162-72.

   Insert

   Rubicon (RUBCN Human)
   

   Tags

   6xHis-tag and Trigger Factor (TF)

   Expression

   Bacterial

   Available Since

   April 17, 2015

   Availability

   Academic Institutions and Nonprofits only

2. GFP-CaM-uTEVp

   Plasmid

   #163032

   Purpose

   FLiCRE protease for neuronal expression

   Depositor

   Alice Ting

   Article

   Kim et al Cell. 2020 Dec 23;183(7):2003-2019.e16.

   Insert

   GFP-CaM-uTEVp
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   Synapsin

   Available Since

   Jan. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBSKΔB- CAG-rtTA2sM2-IRES-tTSkid-IRES-Neo

   Plasmid

   #62346

   Purpose

   Expresses rtTA2sM2 and tTSkid in mammalian cells

   Depositor

   Hiroshi Ochiai

   Article

   Ochiai et al Nucleic Acids Res. 2015 Jun 19. pii: gkv

   Insert

   rtTA2sM2-IRES-tTSkid
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   June 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRK5-EGFP-Tau E14

   Plasmid

   #46907

   Purpose

   expresses EGFP tagged Tau E14 in mammalian cells

   Depositor

   Karen Ashe

   Article

   Hoover et al Neuron. 2010 Dec 22;68(6):1067-81. doi:

   Insert

   Tau E14 (MAPT Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   all 14 S/P or T/P amino acid residues (T111, T153…

   Promoter

   CMV

   Available Since

   July 24, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. mRFP-Clc

   Plasmid

   #14435

   Depositor

   Ari Helenius

   Article

   Tagawa et al J Cell Biol. 2005 Aug 29. 170(5):769-79.

   Insert

   Clathrin light chain (Clc Mouse)
   

   Tags

   mRFP

   Expression

   Mammalian

   Available Since

   March 23, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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6. TetOn-CLIMP63-mCherry-eGFP

   Plasmid

   #139871

   Purpose

   Dox-inducible lentiviral expression of human CLIMP63

   Depositor

   Jacob Corn

   Article

   Liang et al Cell. 2020 Mar 19;180(6):1160-1177.e20.

   Insert

   CLIMP63 (CKAP4 Human)
   

   Use

   Lentiviral

   Tags

   mCherry-eGFP

   Available Since

   March 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLPCX mito roGFP2-Orp1

   Plasmid

   #64992

   Purpose

   Mammalian expression of mitochondrial roGFP2-Orp1 (retroviral vector)

   Depositor

   Tobias Dick

   Article

   Gutscher et al J Biol Chem. 2009 Nov 13;284(46):31532-4

   Insert

   Orp1 (HYR1 Budding Yeast)
   

   Use

   Retroviral

   Tags

   roGFP2

   Expression

   Mammalian

   Mutation

   mitochondrial targeting sequence

   Available Since

   June 29, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3-DN-hCUL3-FLAG

   Plasmid

   #15820

   Depositor

   Wade Harper

   Article

   Jin et al Methods Enzymol. 2005;399:287-309. doi:

   Insert

   Cullin3 (CUL3 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Dominant negative: N418

   Available Since

   Sept. 28, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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9. mRuby-N1

   Plasmid

   #54581

   Purpose

   Localization: N1 Cloning Vector, Excitation: 558, Emission: 605

   Depositor

   Michael Davidson

   Type

   Empty backbone

   Tags

   mRuby

   Expression

   Mammalian

   Available Since

   June 12, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pCSDest-HA-TMPRSS2

    Plasmid

    #154963

    Purpose

    TMPRSS2 with N-terminal HA

    Depositor

    Utpal Pajvani

    Article

    Bartolome et al Sci Rep. 2021 May 7;11(1):9803. doi: 10.

    Insert

    HA-TMPRSS2 (TMPRSS2 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    July 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pCDNA3.1-hMcl-1

    Plasmid

    #25375

    Depositor

    Roger Davis

    Article

    Morel et al Mol Cell Biol. 2009 Jul . 29(14):3845-52

    Insert

    MCL1 (MCL1 Human)
    

    Tags

    V5 and His tag

    Expression

    Mammalian

    Available Since

    Aug. 23, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLentiCRISPR-mCherry

    Plasmid

    #75161

    Purpose

    Derived from LentiCRISPR v1 but expresses mCherry instead of puromycin resistance. 3rd generation.

    Depositor

    Beat Bornhauser

    Article

    McComb et al Sci Transl Med. 2016 May 18;8(339):339ra

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Available Since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pUCmini-iCAP-PHP.V1

    Plasmid

    #127847

    Purpose

    non-standard AAV2 rep-AAV-PHP.V1 cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

    Depositor

    Viviana Gradinaru

    Article

    Ravindra Kumar et al Nat Methods. 2020 May;17(5):541-550. doi

    Insert

    Synthetic construct isolate AAV-PHP.V1 VP1 gene
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    p41

    Available Since

    April 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. HA-FOXO3a WT

    Plasmid

    #1787

    Depositor

    Michael Greenberg

    Article

    Brunet et al Cell 1999 Mar 19;96(6):857-68.

    Insert

    FOXO3a (FOXO3 Human)
    

    Tags

    HA (YDVPDYASL)

    Expression

    Mammalian

    Available Since

    Dec. 7, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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15. pPP085

    Plasmid

    #158795

    Purpose

    pRSF-Duet1 derived plasmid containing C-terminally hexa-histidine tagged CasΦ-2 in MCS1 for expression in E. coli and protein purification

    Depositor

    Jennifer Doudna

    Article

    Pausch et al Science. 2020 Jul 17;369(6501):333-337.

    Insert

    CasPhi-2
    

    Tags

    6xHis

    Expression

    Bacterial

    Available Since

    Aug. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET Biotin His6 mCherry LIC cloning vector (H6-mCherry)

    Plasmid

    #29722

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Tags

    6xHis, AviTag (biotin), TEV cleavage site, and mC…

    Expression

    Bacterial

    Promoter

    T7-lacO (lactose/IPTG inducible)

    Available Since

    June 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJMP3

    Plasmid

    #79875

    Purpose

    Bacillus subtilis sgRNA expression vector; integrates into thrC

    Depositor

    Carol Gross, Jason Peters, Stanley Qi

    Article

    Peters et al Cell. 2016 Jun 2;165(6):1493-506. doi: 1

    Insert

    sgRNA RR1
    

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    veg

    Available Since

    Sept. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. Sumo3 PolQM1

    Plasmid

    #78462

    Purpose

    Inducible expression in E. coli

    Depositor

    Sylvie Doublie, Susan Wallace

    Article

    Hogg et al J Mol Biol. 2011 Jan 21;405(3):642-52. d

    Insert

    Polymerase theta (POLQ Human)
    

    Tags

    His and SUMO3

    Expression

    Bacterial

    Mutation

    delta 1-1791; Q2513R (please see depositor commen…

    Promoter

    T7

    Available Since

    July 28, 2016

    Availability

    Academic Institutions and Nonprofits only

19. pCS6-YFP-SMASh

    Plasmid

    #68853

    Purpose

    Expresses SMASh tag fused to YFP at C terminus in mammalian cells

    Depositor

    Michael Lin

    Article

    Chung et al Nat Chem Biol. 2015 Jul 27. doi: 10.1038

    Insert

    YFP-SMASh
    

    Tags

    SMASh tag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLdCN

    Plasmid

    #84290

    Purpose

    Express gRNA and Cas9 in Leishmania with Neomycin resistance

    Depositor

    Greg Matlashewski

    Article

    Zhang et al mSphere. 2017 Jan 18;2(1). pii: e00340-1

    Insert

    gRNA and Cas9
    

    Use

    CRISPR; Leishmania

    Promoter

    L. donovani ribosome RNA promoter

    Available Since

    Jan. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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