19,824 results

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GFP-PTEN

Plasmid

#13039

Depositor

Alonzo Ross

Article

Liu et al J Cell Biochem. 2005 Oct 1. 96(2):221-34

Insert

PTEN (PTEN Human)

Tags

EGFP

Expression

Mammalian

Available Since

Nov. 3, 2006

Availability

Academic Institutions and Nonprofits only

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pSpCTRE-CD4

Plasmid

#114010

Purpose

Dox-inducible Cas9 expression with a CD4 selection marker

Depositor

Charles Rudin

Article

Hulton et al Nat Cancer. 2020 Mar;1(3):359-369. doi:

Insert

SpCas9

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

March 10, 2020

Availability

Academic Institutions and Nonprofits only

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HA-HIF2alpha-P405A/P531A-pBabe-puro

Plasmid

#19006

Depositor

William Kaelin

Article

Yan et al Mol Cell Biol. 2007 Mar . 27(6):2092-102

Insert

Hypoxia inducible factor 2 alpha (EPAS1 Human)

Use

Retroviral

Tags

HA-tag

Expression

Mammalian

Mutation

cDNA changed amino acids: P405A and P531A

Available Since

Aug. 20, 2008

Availability

Academic Institutions and Nonprofits only

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pAAV.hSyn.FLEX.iGluSnFR3.v857.GPI.codonopt

Plasmid

#175181

Purpose

Fluorescent reporter for glutamate, third generation, variant 857 in GPI backbone. iGluSnFR3.v857.GPI

Depositor

Kaspar Podgorski

Article

Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4

Has Service

AAV1

Insert

pAAV hSyn FLEX iGluSnFR3 v857.GPI

Use

AAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…

Tags

GPI Anchor, IgK-chain, and Myc epi tag

Expression

Mammalian

Promoter

human Synapsin

Available Since

Oct. 22, 2021

Availability

Academic Institutions and Nonprofits only

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FU-tet-o-hKLF4

Plasmid

#19777

Depositor

Konrad Hochedlinger

Article

Maherali et al Cell Stem Cell. 2008 Sep 11. 3(3):340-5.

Insert

Klf4 (KLF4 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 23, 2009

Availability

Academic Institutions and Nonprofits only

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SpCas9-NG

Plasmid

#138566

Purpose

Expresses human codon-optimized rationally engineered SpCas9 variant and blasticidin resistance: EFS promoter-SpCas9-NG-NLS-FLAG-P2A-BSD

Depositor

Hyongbum Kim

Article

Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

Insert

SpCas9-NG

Use

CRISPR and Lentiviral

Tags

BSD, FLAG, and NLS

Expression

Mammalian

Mutation

L1111R, D1135V, G1218R, E1219F, A1322R, R1335V, T…

Promoter

EFS

Available Since

June 9, 2020

Availability

Academic Institutions and Nonprofits only

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pLIX403_UBAP2L_mCherry

Plasmid

#105286

Purpose

puro-selectable lentivirus vector for inducible expression of a UBAP2L-mCherry fusion protein

Depositor

Eugene Yeo

Article

Markmiller et al Cell. 2018 Jan 25;172(3):590-604.e13. do

Insert

UBAP2L (UBAP2L Human)

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Promoter

TRE promoter, Tet ON

Available Since

Sept. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pCEP4-myc-HLA-B7

Plasmid

#135509

Purpose

Mammalian expression of myc-tagged HLA-B*07:02

Depositor

Erik Procko

Article

McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

Insert

HLA-B*07:02 (HLA-B Human)

Tags

Extracellular c-myc epitope tag and Signal peptid…

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pHAGE-XPO1

Plasmid

#116804

Purpose

Lentiviral expression of XPO1

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

XPO1 (XPO1 Human)

Use

Lentiviral

Mutation

WT

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-ASAP3-WPRE

Plasmid

#132331

Purpose

expresses ASAP3(ASAP-family genetically encoded voltage indicator) in mammalian cells, gene delivered by pAAV-hSyn vector

Depositor

Michael Lin

Article

Villette et al Cell. 2019 Dec 12;179(7):1590-1608.e23.

Insert

ASAP3

Use

AAV

Expression

Mammalian

Promoter

hSynapsin

Available Since

Dec. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pLPCX roGFP2-Orp1

Plasmid

#64991

Purpose

Mammalian expression of cytosolic roGFP2-Orp1 (retroviral vector)

Depositor

Tobias Dick

Article

Gutscher et al J Biol Chem. 2009 Nov 13;284(46):31532-4

Insert

Orp1 (HYR1 Budding Yeast)

Use

Retroviral

Tags

roGFP2

Expression

Mammalian

Available Since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

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pLenti6.2_mCherry_Grx1_roGFP2

Plasmid

#155045

Purpose

Fluorescent redox reporter

Depositor

Vanessa LaPointe

Insert

Glutaredoxin-1 (GLRX Human)

Use

Lentiviral

Tags

roGFP2

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 4, 2020

Availability

Academic Institutions and Nonprofits only

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Puro-Cas9 donor

Plasmid

#58409

Purpose

Donor vector for genomic targeting of a Tetracycline-inducible Cas9 cassette to the human AAVS1/PPP1R12C locus

Depositor

Danwei Huangfu

Article

Gonzalez et al Cell Stem Cell. 2014 Jun 11. pii: S1934-

Insert

hSpCas9

Use

CRISPR

Tags

3xFlag, Nucleoplasmin NLS, and SV40 NLS

Expression

Mammalian

Available Since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only

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pRK7-HA-S6K1-KR

Plasmid

#8985

Depositor

John Blenis

Article

Schalm et al Curr Biol. 2002 Apr 16. 12(8):632-9.

Insert

S6K1 (Rps6kb1 Rat)

Tags

HA

Expression

Mammalian

Mutation

K100R, kinase-inactive

Available Since

Jan. 4, 2006

Availability

Academic Institutions and Nonprofits only

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pCMV3flag8HOIL-1L

Plasmid

#50016

Purpose

Mammalian expression of human HOIL-1L (RBCK1) with a c-terminal 3xFLAG tag, and hygromycin selection.

Depositor

Martin Dorf

Article

Fu et al Cell Res. 2013 Sep 24. doi: 10.1038/cr.2

Insert

RanBP-type and C3HC4-type zinc finger containing 1 HOIL-1L (RBCK1 Human)

Tags

3 x FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 31, 2013

Availability

Academic Institutions and Nonprofits only

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Lv EF1a dCas9-HA 2A Blast

Plasmid

#102812

Purpose

Lentiviral plasmid from FIRE-Cas9 system to express dCas9-HA fusion protein

Depositor

Jerry Crabtree

Article

Braun et al Nat Commun. 2017 Sep 15;8(1):560. doi: 1

Insert

dCas9-HA

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

D10A and N863A (dCas9)

Promoter

EF1a

Available Since

Jan. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pY095

Plasmid

#84744

Purpose

Expresses huLbCpf1-T2A-GFP and crRNA guide

Depositor

Feng Zhang

Article

Zetsche et al Nat Biotechnol. 2017 Jan;35(1):31-34. do

Inserts

huLbCpf1
GFP

Use

CRISPR

Tags

3xHA, NLS, and T2A

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

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lenti F9-TetR-EGFP-IRES-PuroR

Plasmid

#117049

Purpose

lentiviral vector for expression of TetR-EGFP, for visualization of TetO repeats

Depositor

Huimin Zhao

Article

Tasan et al Nucleic Acids Res. 2018 Jun 15. pii: 503

Insert

F9-TetR-EGFP

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

F9 promoter

Available Since

March 7, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-MMLVgag-3xNES-ABE7.10-NG

Plasmid

#181753

Purpose

MMLV-Gag fused to 3xNES-ABE7.10-NG with TSTLLMENSS cleavage site between Gag-NES and ABE. For production of virus like particles with base-editor RNP cargo.

Depositor

David Liu

Article

Banskota et al Cell. 2022 Jan 7. pii: S0092-8674(21)014

Insert

MMLVgag-3xNES-ABE7.10-NG

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 8, 2022

Availability

Academic Institutions and Nonprofits only

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1481 pcDNA3 flag HA 14-3-3 zeta

Plasmid

#9002

Depositor

William Sellers

Insert

14-3-3 zeta (YWHAZ Bovine)

Tags

HA and flag

Expression

Mammalian

Mutation

T226A

Available Since

April 20, 2006

Availability

Academic Institutions and Nonprofits only

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