We narrowed to 2,399 results for: puc19

Showing: 1141 - 1170 of 2399 results

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pSW071

Plasmid

#140037

Purpose

PCC 11901 fadD locus KO with a kanamycin resistance cassette

Depositor

Peter Nixon

Article

Wlodarczyk et al Commun Biol. 2020 May 7;3(1):215. doi: 1

Insert

Kanamycin resistance gene

Use

Gene knockout in cyanobacteria

Promoter

KanR

Available Since

April 17, 2020

Availability

Academic Institutions and Nonprofits only

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pUC-UAS1B16-TEF(504)

Plasmid

#44371

Depositor

Hal Alper

Article

Blazeck et al Appl Environ Microbiol. 2011 Nov;77(22):

Insert

UAS1B16-TEF(504) promoter

Use

Synthetic Biology

Expression

Bacterial

Mutation

N/A

Promoter

UAS1B16-TEF(504)

Available Since

July 15, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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pEXPYR

Plasmid

#70030

Purpose

High yield protein secretion in Aspergillus nidulans

Depositor

Rolf Prade

Article

Segato et al Enzyme Microb Technol. 2012 Jul 15;51(2)

Type

Empty backbone

Use

Synthetic Biology

Promoter

A. niger glucoamylase promoter (glaAp)4

Available Since

Dec. 16, 2015

Availability

Academic Institutions and Nonprofits only

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pUC-UAS1B8-TEF(136)

Plasmid

#44380

Depositor

Hal Alper

Article

Blazeck et al Appl Environ Microbiol. 2011 Nov;77(22):

Insert

UAS1B8-TEF(136) promoter

Use

Synthetic Biology

Expression

Bacterial

Mutation

N/A

Promoter

UAS1B8-TEF(136)

Available Since

April 17, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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pUC-UAS1B8-Leum

Plasmid

#44316

Depositor

Hal Alper

Article

Blazeck et al Appl Environ Microbiol. 2011 Nov;77(22):

Insert

UAS1B8-Leum promoter

Use

Synthetic Biology

Expression

Bacterial

Mutation

N/A

Promoter

UAS1B8-Leum

Available Since

April 18, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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pJBL7005

Plasmid

#136944

Purpose

Malachite green aptamer with stability hairpin, T7 promoter

Depositor

Julius Lucks

Article

Silverman et al ACS Synth Biol. 2019 Feb 15;8(2):403-414

Insert

Malachite green aptamer

Use

Synthetic Biology

Expression

Bacterial

Promoter

T7

Available Since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pUC-UAS1B16-TEF(272)

Plasmid

#44375

Depositor

Hal Alper

Article

Blazeck et al Appl Environ Microbiol. 2011 Nov;77(22):

Insert

UAS1B16-TEF(272) promoter

Use

Synthetic Biology

Expression

Bacterial

Mutation

N/A

Promoter

UAS1B16-TEF(272)

Available Since

April 18, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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pMT1002

Plasmid

#8621

Depositor

Robert Hartley

Article

Hartley et al Protein Peptide Letters 1996 3:225-231

Insert

barnase, barstar

Tags

as pMT416

Expression

Bacterial

Mutation

CI157 lambda repressor gene has two HindIII sites…

Available Since

Jan. 17, 2007

Availability

Academic Institutions and Nonprofits only
pSCW02

Plasmid

#72301

Purpose

Starting substrate for in vitro DNA mismatch repair substrate monitored by FauI cleavage. Derived from pUC19CPDrev H. HWang and J.B. Hays 2002 JBC 277:26136.

Depositor

Peggy Hsieh

Article

Geng et al Anal Biochem. 2011 Jun 15;413(2):179-84.

Type

Empty backbone

Use

Derivative of puc19

Expression

Bacterial

Promoter

lac

Available Since

Aug. 29, 2019

Availability

Academic Institutions and Nonprofits only

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pMH220

Plasmid

#122836

Purpose

dCas9 with GreenGate B-C flanking sequences

Depositor

Markus Schmid

Article

Lee et al PLoS One. 2019 Sep 26;14(9):e0222778. do

Insert

Nuclease-dead SpCas9 (dCas9), codon-optimised for Arabidopsis

Use

Golden gate compatible cloning vector

Mutation

Contains D10A and H840A mutations

Available Since

March 26, 2019

Availability

Academic Institutions and Nonprofits only

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pUC-UAS1B8-TEF(272)

Plasmid

#44376

Depositor

Hal Alper

Article

Blazeck et al Appl Environ Microbiol. 2011 Nov;77(22):

Insert

UAS1B8-TEF(272) promoter

Use

Synthetic Biology

Expression

Bacterial

Mutation

N/A

Promoter

UAS1B8-TEF(272)

Available Since

April 16, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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pGCG26

Plasmid

#82371

Purpose

YFP expressed from pJ23119 in the glpK locus with gentamicin resistance

Depositor

Brian Pfleger

Article

Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

Insert

YFP

Promoter

PJ23119

Available Since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only

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EZH2_N_FS_v2_Donor

Plasmid

#79901

Purpose

N-terminal tagging of human EZH2 with 3xFLAG_Twin_Strep

Depositor

Yannick Doyon

Article

Dalvai et al Cell Rep. 2015 Oct 7. pii: S2211-1247(15

Type

Empty backbone

Use

CRISPR and TALEN

Tags

3xFLAG_Twin_Strep

Available Since

July 19, 2016

Availability

Academic Institutions and Nonprofits only

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pGGF012

Plasmid

#48849

Purpose

Provides a sulfadiazine resistance cassette as GreenGate module.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

pMAS::SulfadiazineR::t35S

Use

Golden gate compatible cloning vector

Mutation

internal BsaI recognition sites in SulfR removed …

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pTX1-RNA0

Plasmid

#126040

Purpose

In-vitro-transcription of control RNA0 in NMR tube for "Systems NMR" analysis

Depositor

Frédéric Allain

Article

Nikolaev et al Nat Methods. 2019 Aug;16(8):743-749. doi

Insert

RNA0

Available Since

May 25, 2021

Availability

Academic Institutions and Nonprofits only

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pUC-UAS1B4-Leum

Plasmid

#44312

Depositor

Hal Alper

Article

Blazeck et al Appl Environ Microbiol. 2011 Nov;77(22):

Insert

UAS1B4-Leum promoter

Use

Synthetic Biology

Expression

Bacterial

Mutation

N/A

Promoter

UAS1B4-Leum

Available Since

July 15, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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pUC-UAS1B8-TEF(504)

Plasmid

#44372

Depositor

Hal Alper

Article

Blazeck et al Appl Environ Microbiol. 2011 Nov;77(22):

Insert

UAS1B8-TEF(504) promoter

Use

Synthetic Biology

Expression

Bacterial

Mutation

N/A

Promoter

UAS1B8-TEF(504)

Available Since

July 15, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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pUC-UAS1B28-Leum

Plasmid

#44327

Depositor

Hal Alper

Article

Blazeck et al Appl Environ Microbiol. 2011 Nov;77(22):

Insert

UAS1B28-Leum promoter

Use

Synthetic Biology

Expression

Bacterial

Mutation

N/A

Promoter

UAS1B28-Leum

Available Since

April 18, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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pCas49

Plasmid

#82391

Purpose

rbcL-sfGFP expressed from PccmK2 promoter with Gmr in glpK

Depositor

Brian Pfleger

Article

Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

Insert

rbcL-GFP

Promoter

PccmK2

Available Since

Sept. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pUC-UAS1B16-Leum

Plasmid

#44324

Depositor

Hal Alper

Article

Blazeck et al Appl Environ Microbiol. 2011 Nov;77(22):

Insert

UAS1B16-Leum promoter

Use

Synthetic Biology

Expression

Bacterial

Mutation

N/A

Promoter

UAS1B16-Leum

Available Since

May 23, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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pAD01 S. cerevisiae telomerase RNA KBS

Plasmid

#61890

Purpose

Plasmid for run-off in vitro transcription of Saccharomyces cerevisiae KBS RNA

Depositor

Thomas Cech

Article

Dalby et al RNA. 2013 Jun;19(6):841-51. doi: 10.1261

Insert

Telomerase RNA hairpin

Promoter

T7

Available Since

March 13, 2015

Availability

Academic Institutions and Nonprofits only
AP582-2

Plasmid

#70049

Purpose

Expresses Ollas::mCherry::linker::H2B::V5 in bacteria

Depositor

Geraldine Seydoux

Article

Paix et al Genetics. 2015 Sep;201(1):47-54. doi: 10

Insert

mCherry::linker::H2B

Tags

Ollas and V5

Expression

Bacterial

Available Since

Oct. 22, 2015

Availability

Academic Institutions and Nonprofits only

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pILGFP4M

Plasmid

#104508

Purpose

Used to evaluate the expression output of Saccharomyces eubayanus GAL2 promoter with yEGFP used as the reporter gene

Depositor

Claudia Vickers

Article

Peng et al ACS Synth Biol. 2018 Feb 16;7(2):748-751

Insert

SeGAL2 promoter-yEGFP

Expression

Yeast

Available Since

April 27, 2022

Availability

Academic Institutions and Nonprofits only

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pCfB8792

Plasmid

#126910

Purpose

Template for PCR amplification of ADE2 gRNA biobrick for targeting Saccharomyces cerevisiae ADE2 gene

Depositor

Irina Borodina

Article

Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

Insert

ADE2 gRNA

Use

Synthetic Biology

Available Since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pGGF002

Plasmid

#48843

Purpose

Provides a BASTA resistance cassette as GreenGate module.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

p35S::BastaR::t35S

Use

Golden gate compatible cloning vector

Mutation

internal BsaI recognition site in 35S promoter re…

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pUC-UAS1B16-TEF(203)

Plasmid

#44377

Depositor

Hal Alper

Article

Blazeck et al Appl Environ Microbiol. 2011 Nov;77(22):

Insert

UAS1B16-TEF(203) promoter

Use

Synthetic Biology

Expression

Bacterial

Mutation

N/A

Promoter

UAS1B16-TEF(203)

Available Since

April 16, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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AP588-1

Plasmid

#71278

Purpose

Expresses myc::TEV::mTagGFP::TEV::3Xflag in bacteria

Depositor

Geraldine Seydoux

Article

Paix et al Genetics. 2015 Sep;201(1):47-54. doi: 10

Insert

tagGFP

Tags

TEV::3xFlag and myc::TEV

Expression

Bacterial

Available Since

Dec. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pILGFP1F5

Plasmid

#165064

Purpose

Characterisation of TEF1 promoter using yEGFP as reporter gene and PGK1 terminator

Depositor

Claudia Vickers

Article

Lu et al Nat Commun. 2021 Feb 16;12(1):1051. doi:

Insert

P-URA3>KlURA3>T-AgTEF1-P-TEF1> (BamHI)yEGFP>T-PGK1-T-URA3

Expression

Yeast

Mutation

WT

Available Since

March 16, 2021

Availability

Academic Institutions and Nonprofits only

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pAP1638

Plasmid

#137089

Purpose

Plasmid for PCR-generated donor DNA for rapid tagging of proteins with 4*(GFP11)

Depositor

Geraldine Seydoux

Article

Paix et al Curr Protoc Mol Biol. 2019 Dec;129(1):e1

Insert

4*(GFP11)

Use

Pcr template for donor dna

Expression

Bacterial

Available Since

March 13, 2020

Availability

Academic Institutions and Nonprofits only

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pGGF008

Plasmid

#48848

Purpose

Provides a BASTA resistance cassette as GreenGate module.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

pNOS::BastaR::tNOS

Use

Golden gate compatible cloning vector

Mutation

chi sequence in BastaR removed by silent substitu…

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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