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dCas13d-dsRBD-APEX2

Plasmid

#154939

Purpose

TetON-APEX2-V5-BPNLS-dRfxCas13d-dsRBD-BPNLS-P2A-GFP

Depositor

Alice Ting

Publication

Han et al Proc Natl Acad Sci U S A. 2020 Aug 24. p

Insert

TetON-APEX2-V5-BPNLS-dRfxCas13d-dsRBD-BPNLS-P2A-GFP

Tags

V5

Expression

Mammalian

Promoter

TRE3G

Available since

Sept. 4, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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AbVec2.0-IGHG1

Plasmid

#80795

Purpose

Expression of secretory immunoglobulin heavy chains in mammalian cells, human IgG1 isotype

Depositor

Hedda Wardemann

Publication

Tiller et al J Immunol Methods. 2008 Jan 1;329(1-2):1

Type

Empty backbone

Expression

Mammalian

Promoter

hCMV IE1 gene promoter

Available since

June 28, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

37 Articles

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iCas

Plasmid

#84232

Purpose

Expression of SpCas9 with 4 ERT2 fusion protein and empty gRNA cassette. The activity of Cas9 can be switched on and off in human cells with 4-hydroxytamoxifen (4-HT)

Depositor

Meng How Tan

Publication

Liu et al Nat Chem Biol. 2016 Sep 12. doi: 10.1038

Insert

Cas9

Tags

2A-OFP co-expression and ERT2-ERT2

Expression

Mammalian

Promoter

CMV

Available since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pRK792

Plasmid

#8830

Depositor

David Waugh

Publication

Kapust et al Protein Eng. 2001 Dec . 14(12):993-1000.

Insert

TEV protease, S219P mutant

Tags

His, MBP (cleaved), and polyarginine

Expression

Bacterial

Mutation

S219P mutation

Available since

July 18, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pBABE hBRM

Plasmid

#1961

Depositor

Robert Kingston

Publication

Sif et al Genes Dev 2001 Mar 1;15(5):603-18.

Insert

BRM (SMARCA2 Human)

Use

Retroviral

Tags

flag

Expression

Mammalian

Available since

March 23, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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sortase A pentamutant (eSrtA) in pET29

Plasmid

#75144

Purpose

evolved sortase A (eSrtA) pentamutant with improved kinetics and activity

Depositor

David Liu

Publication

Chen et al Proc Natl Acad Sci U S A. 2011 Jul 12;10

Insert

sortase A pentamutant

Tags

6x His

Expression

Bacterial

Mutation

P94R, D160N, D165A, K190E, K196T. Deleted amino a…

Promoter

T7

Available since

Aug. 23, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

36 Articles

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pCMV-MMLVgag-3xNES-Cas9

Plasmid

#181752

Purpose

MMLV-Gag fused to 3x NES-SpyCas9 with the TSTLLMENSS cleavage site in between NES and Cas9. For production of virus like particles with Cas9 nuclease RNP cargo.

Depositor

David Liu

Publication

Banskota et al Cell. 2022 Jan 7. pii: S0092-8674(21)014

Insert

MMLV-Gag-3xNES-Cas9 (Spy)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available since

Feb. 8, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pCS2+HyPer7-NES

Plasmid

#136467

Purpose

Mammalian expression of cytoplasm targeted ultrasensitive hydrogen peroxide indicator HyPer7 for optical imaging

Depositor

Vsevolod Belousov

Publication

Pak et al Cell Metab. 2020 Mar 3;31(3):642-653.e6.

Insert

HyPer7

Tags

Nuclear export signal from the HIV Rev protein

Expression

Mammalian

Promoter

CMV, SP6

Available since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

17 Articles

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wtTDP43tdTOMATOHA

Plasmid

#28205

Depositor

Zuoshang Xu

Publication

Yang et al PLoS One. 2010 . 5(12):e15878.

Insert

Tar DNA binding protein (TARDBP Human)

Tags

HA tag and tdtomato

Expression

Mammalian

Available since

June 1, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

18 Articles

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pMXs-DsRed Express

Plasmid

#22724

Depositor

Shinya Yamanaka

Publication

Hong et al Nature. 2009 Aug 27. 460(7259):1132-5.

Insert

a variant of Discosoma sp. red fluorescent protein

Use

Retroviral

Expression

Mammalian

Mutation

N/A

Available since

Dec. 10, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

14 Articles

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pST1374

Plasmid

#13426

Depositor

Keith Joung

Publication

Wright et al Nat Protoc. 2006;1(3):1637-52. doi: 10.1

Type

Empty backbone

Tags

Flag

Expression

Mammalian

Available since

Feb. 8, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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pMXs-c-Myc-T58A

Plasmid

#13372

Purpose

Retroviral expression of mouse c-Myc T58A mutant

Depositor

Shinya Yamanaka

Publication

Takahashi et al Cell. 2006 Aug 25. 126(4):663-76.

Insert

myelocytomatosis oncogene (T58A mutant) (Myc Mouse)

Use

Retroviral

Expression

Mammalian

Mutation

Tyrosine 58 to Alanine

Available since

Jan. 12, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pEF5B-FRT-GFP-αTAT1

Plasmid

#27099

Depositor

Maxence Nachury

Publication

Shida et al Proc Natl Acad Sci U S A. 2010 Dec 14;10

Insert

α-Tubulin K40 acetyltransferase1 (ATAT1 Human)

Tags

GFP, S-tag, and TEV site

Expression

Mammalian

Promoter

EF1a

Available since

Jan. 5, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

16 Articles

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pEG BacMam

Plasmid

#160451

Purpose

vector optimized for use in screening assays, as well as for efficient production of baculovirus and robust expression of the target protein

Depositor

Eric Gouaux

Publication

Goehring et al Nat Protoc. 2014 Nov;9(11):2574-85. doi:

Type

Empty backbone

Expression

Mammalian

Promoter

CMV

Available since

Dec. 9, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

14 Articles

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paavCAG-iCre

Plasmid

#51904

Purpose

For AAV expression of iCre recombinase

Depositor

Jinhyun Kim

Publication

Druckmann et al Neuron. 2014 Feb 5;81(3):629-40. doi: 10

Insert

improved Cre recombinase

Use

AAV and Cre/Lox

Promoter

CAG

Available since

April 15, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

19 Articles

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pEBG‐AMPK α1(1‐312)

Plasmid

#27632

Depositor

Reuben Shaw

Publication

Egan et al Science. 2011 Jan 28;331(6016):456-61. d

Insert

AMPK α1(1‐312) (Prkaa1 Rat)

Tags

GST

Expression

Mammalian

Mutation

Constitutively  active  by  truncation  after  am…

Available since

May 18, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

12 Articles

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pAG426GPD-ccdB

Plasmid

#14156

Depositor

Susan Lindquist

Publication

Alberti et al Yeast. 2007 Oct . 24(10):913-9.

Type

Empty backbone

Use

Gateway Cloning

Expression

Yeast

Available since

March 16, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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mCherry

Plasmid

#176016

Purpose

Mammalian expression of mCherry. Parton lab clone DFQ

Depositor

Rob Parton

Publication

Lo et al J Cell Biol. 2021 Dec 6;220(12). pii: 21

Insert

mCherry

Expression

Mammalian

Available since

Nov. 4, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pHAGE-TO-nls-st1dCas9-3nls-3XTagBFP2

Plasmid

#64512

Purpose

Expression of St1 dCas9-3XTagBFP2 in mammalian cells

Depositor

Thoru Pederson

Publication

Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

Insert

St1 dCas9

Use

CRISPR and Lentiviral

Tags

3XmTagBFP2

Expression

Mammalian

Promoter

CMV-TO

Available since

May 1, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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Hsp68-LacZ-Gateway

Plasmid

#37843

Depositor

Nadav Ahituv

Publication

Pennacchio et al Nature. 2006 Nov 23;444(7118):499-502. E

Type

Empty backbone

Use

Mouse Targeting

Promoter

Mouse Hsp68 minimal promoter

Available since

Sept. 20, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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