We narrowed to 16,699 results for: IGH@;

1. pET20b-YbAnbu

   Plasmid

   #129637

   Purpose

   Expression plasmid of Anbu from Y. bercovieri (UNIPROT A0A0T9RXH3 variant with V14A and G107H mutations)

   Depositor

   Matthias Bochtler

   Article

   Piasecka et al J Mol Biol. 2018 Mar 2;430(5):611-627. d

   Insert

   YbAnbu
   

   Use

   Tags

   His6-tag

   Expression

   Bacterial

   Mutation

   V14A and G107H

   Promoter

   T7

   Available since

   Nov. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET20b-YbAnbuT1A

   Plasmid

   #129638

   Purpose

   Expression plasmid of Anbu from Y. bercovieri (inactive mutant; (UNIPROT A0A0T9RXH3 variant with T1A, V14A and G107H mutations)

   Depositor

   Matthias Bochtler

   Article

   Piasecka et al J Mol Biol. 2018 Mar 2;430(5):611-627. d

   Insert

   YbAnbuT1A
   

   Use

   Tags

   His6-tag

   Expression

   Bacterial

   Mutation

   T1A, V14A and G107H

   Promoter

   T7

   Available since

   Nov. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. AAVS1-Bxb1-LP-TC

   Plasmid

   #183754

   Purpose

   AAVS1 targeting construct with the Bxb1 landing pad cassette.

   Depositor

   Richard Davis

   Article

   Blanch-Asensio et al Cell Rep Methods. 2022 Sep 22;2(10):1003

   Insert

   loxP-eGFP-attP-Bsd-lox257
   

   Use

   Cre/Lox and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCSII-EF/mt-(n1)StayGold

   Plasmid

   #185823

   Purpose

   A soluble photostable fluorescent marker of the mitochondrial matrix. The StayGold gene has codon optimization for mammalian expression.

   Depositor

   Atsushi Miyawaki

   Article

   Hirano et al Nat Biotechnol. 2022 Apr 25. pii: 10.103

   Insert

   mt-(n1)StayGold
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   July 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. EGFP-CLC

   Plasmid

   #193559

   Purpose

   Expresses human Clathrin Light Chain B tagged with mEGFP in mammalian cells.

   Depositor

   Justin Taraska

   Article

   Obashi et al Nat Commun. 2023 Feb 9;14(1):732. doi: 1

   Insert

   Clathrin light chain B (CLTB Human)
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. Sniper2L

   Plasmid

   #193856

   Purpose

   Expresses human codon-optimized Sniper2L and blasticidin resistance: EFS promoter-Sniper2L-NLS-FLAG-P2A-BSD

   Depositor

   Hyongbum Kim

   Article

   Kim et al Nat Chem Biol. 2023 Mar 9. doi: 10.1038/

   Insert

   Sniper2L
   

   Use

   CRISPR and Lentiviral

   Tags

   BSD, FLAG, and NLS

   Expression

   Mammalian

   Mutation

   E1007L

   Promoter

   EFS

   Available since

   March 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV.CAG.Flex.Ruby2sm-Flag.WPRE.SV40

   Plasmid

   #98928

   Purpose

   Cre dependent AAV expression of Ruby2 spaghetti monster FLAG construct

   Depositor

   Loren Looger

   Article

   Viswanathan et al Nat Methods. 2015 Jun;12(6):568-76. doi:

   Has Service

   AAV1

   Insert

   Ruby2-smFP-Flag
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Jan. 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLenti-XI-Neo-GST-Constitutively Active AMPK

   Plasmid

   #139843

   Purpose

   Lentiviral expression of GST-tagged Constitutively Active rat AMPK

   Depositor

   Jacob Corn

   Article

   Liang et al Cell. 2020 Mar 19;180(6):1160-1177.e20.

   Insert

   AMPK (Prkaa2 Rat)
   

   Use

   Lentiviral

   Tags

   GST

   Expression

   Mutation

   Promoter

   Available since

   March 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. AAVS1-phiC31-LP-TC

   Plasmid

   #183755

   Purpose

   AAVS1 targeting construct with the phiC31 landing pad cassette.

   Depositor

   Richard Davis

   Article

   Blanch-Asensio et al Cell Rep Methods. 2022 Sep 22;2(10):1003

   Insert

   FRT-mCherry-attP-BleoR-F3
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLenti-XI-Neo-GST- AMPK- Kinase Dead (K to R)

    Plasmid

    #139844

    Purpose

    Lentiviral expression of GST-tagged rat AMPK- Kinase Dead (K to R)

    Depositor

    Jacob Corn

    Article

    Liang et al Cell. 2020 Mar 19;180(6):1160-1177.e20.

    Insert

    AMPK-Kinase-dead (Prkaa2 Rat)
    

    Use

    Lentiviral

    Tags

    GST

    Expression

    Mutation

    K47R

    Promoter

    Available since

    March 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3/er-(n2)oxStayGold(c4)

    Plasmid

    #185822

    Purpose

    A soluble photostable fluorescent marker of the ER lumen. The StayGold gene has no codon optimization for mammalian expression.

    Depositor

    Atsushi Miyawaki

    Article

    Hirano et al Nat Biotechnol. 2022 Apr 25. pii: 10.103

    Insert

    er-(n2)oxStayGold(c4)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRetroX-PTuner DD-linker-M.EcoGII-v5-Lamin B1

    Plasmid

    #122083

    Purpose

    Express M.EcoGII fused to LaminB1 with detabilization domain - inducible

    Depositor

    Laure Crabbe

    Article

    Sobecki et al Cell Rep. 2018 Dec 4;25(10):2891-2903.e5

    Insert

    DD-linker-M.EcoGII-V5-LaminB1
    

    Use

    Retroviral

    Tags

    V5 tag on Nter of LaminB1

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV_hSyn_SpikeyGi2_kv2.1

    Plasmid

    #195313

    Purpose

    Genetically-encoded voltage indicator SpikeyGi2, soma and AIS targeted, in AAV vector under the neuronal-specific promoter hSyn

    Depositor

    Vincent Pieribone

    Article

    Platisa et al Nat Methods. 2023 Mar 27. doi: 10.1038/s

    Insert

    SpikeyGi2
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    hSyn

    Available since

    March 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRSET mNG-GECO1

    Plasmid

    #201933

    Purpose

    Expresses mNG-GECO1 indicator in bacteria

    Depositor

    Robert Campbell

    Article

    Zarowny et al ACS Sens. 2020 Jul 24;5(7):1959-1968. do

    Insert

    mNeonGreenGECO1
    

    Use

    Tags

    HIS tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Bxb1_miRFP703

    Plasmid

    #183759

    Purpose

    Bxb1-specific donor plasmid for intergrating a transcription unit for expression of membrane-localised miRFP703

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al Cell Rep Methods. 2022 Sep 22;2(10):1003

    Insert

    miRFP703
    

    Use

    Tags

    Lck lipidation signal

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pUB-nHyPer2 (NLS:HyPer2:NLS)

    Plasmid

    #84730

    Purpose

    Expresses HyPer2 in plant cells-biosensor for Hydrogen peroxide targeted to Nuclei

    Depositor

    Philip Mullineaux

    Article

    Exposito-Rodriguez et al Nat Commun. 2017 Jun 29;8(1):49. doi: 10

    Insert

    HyPer2 targeted to nucleus
    

    Use

    Tags

    NLS (nuclear localisation signal and NLS (nuclear…

    Expression

    Plant

    Mutation

    Promoter

    ubiqutin- 10 gene promoter (pUBQ10) of Arabidopsis

    Available since

    July 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pENTR-mCherry-attP(C31)-*BleoR

    Plasmid

    #183752

    Purpose

    phiC31 landing pad cassette.

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al Cell Rep Methods. 2022 Sep 22;2(10):1003

    Insert

    FRT-mCherry-attP-BleoR-F3
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. CLC-EGFP

    Plasmid

    #193566

    Purpose

    Expresses human Clathrin Light Chain B tagged with mEGFP in mammalian cells.

    Depositor

    Justin Taraska

    Article

    Obashi et al Nat Commun. 2023 Feb 9;14(1):732. doi: 1

    Insert

    Clathrin light chain B (CLTB Human)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAAV_Flex_syn_SpikeyGi2_kv2.1

    Plasmid

    #195314

    Purpose

    Genetically-encoded voltage indicator SpikeyGi2, soma and AIS targeted, double-flowed in AAV vector under the promoter hSyn

    Depositor

    Vincent Pieribone

    Article

    Platisa et al Nat Methods. 2023 Mar 27. doi: 10.1038/s

    Insert

    SpikeyGi2
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human synapsin promoter

    Available since

    March 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. FCK-ArchT-GFP

    Plasmid

    #31177

    Purpose

    Lentiviral expression of ArchT-GFP for neuronal inhibition

    Depositor

    Edward Boyden

    Article

    Han et al Front Syst Neurosci. 2011;5:18. Epub 201

    Insert

    ArchT-GFP
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CaMKII

    Available since

    Sept. 16, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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