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pLV-EF1alpha-EPO-218-PDGFR

Plasmid

#139057

Purpose

A lentivirus vector to integrate a surface membrane anchored human EPO gene

Depositor

Tao Liu

Article

Liu et al Cell Chem Biol. 2017 Jun 22;24(6):767-77

Insert

erythropoietin(EPO) (EPO Human)

Use

Lentiviral

Available Since

March 4, 2020

Availability

Academic Institutions and Nonprofits only

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pIA1385

Plasmid

#166862

Purpose

Codon optimized SARS-CoV-2 Nsp12 gene was cloned into a modified pRSFDuet-1 vector (Addgene #159107) for expression in E. coli.

Depositor

Irina Artsimovitch

Article

Wang et al mBio. 2021 Jun 29;12(3):e0142321. doi: 1

Insert

SARS-COV-2 Nsp12 (ORF1ab Synthetic)

Tags

His-SUMO

Expression

Bacterial

Promoter

T7

Available Since

March 16, 2021

Availability

Academic Institutions and Nonprofits only

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pXJ40 GADD34 (Delta Pest1)

Plasmid

#78096

Purpose

mammalian expression of GADD34 Delta Pest1

Depositor

Shirish Shenolikar

Article

Choy et al Cell Rep. 2015 Jun 30;11(12):1885-91. do

Insert

GADD34 (PPP1R15A Human)

Tags

FLAG

Expression

Mammalian

Mutation

deletion of PEST1

Promoter

CMV

Available Since

June 21, 2016

Availability

Academic Institutions and Nonprofits only

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mEmerald-PCNA-19

Plasmid

#54221

Purpose

Localization: Nucleus, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Article

Lu et al J Cell Biol. 2010 Jun 14;189(6):967-80.

Insert

PCNA-19 (PCNA Human)

Tags

mEmerald

Expression

Mammalian

Promoter

CMV

Available Since

July 10, 2014

Availability

Industry, Academic Institutions, and Nonprofits
Lmx1b

Plasmid

#34997

Depositor

Malin Parmar

Article

Pfisterer et al Proc Natl Acad Sci U S A. 2011 Jun 21;10

Insert

LIM homeobox transcription factor 1-beta (Lmx1b Mouse)

Use

Lentiviral

Expression

Mammalian

Available Since

March 6, 2012

Availability

Academic Institutions and Nonprofits only

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pMSCV-mCherry-Vav1

Plasmid

#50044

Purpose

Expression of mCherry-Vav1 in mammalian cells

Depositor

Hidde Ploegh

Article

Strijbis et al PLoS Pathog. 2013 Jun;9(6):e1003446. doi

Insert

Vav1 (Vav1 Mouse)

Use

Retroviral

Tags

mCherry

Expression

Mammalian

Promoter

pCMV

Available Since

Jan. 13, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HCMVgH-8his (Merlin)

Plasmid

#136447

Purpose

Mammalian expression plasmid for HCMV glycoprotein H (Merlin strain) extracellular domain with 8his purification tag

Depositor

Erik Procko

Article

Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.

Insert

glycoprotein H (UL75 Human betaherpesvirus 5)

Tags

Short GGSG linker and 8his tag

Expression

Mammalian

Mutation

Extracellular domain (a.a. 1-716). Codon-optimize…

Promoter

CMV

Available Since

July 9, 2020

Availability

Academic Institutions and Nonprofits only

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pCAX-mScarlet-CIP4

Plasmid

#178364

Purpose

Expresses CIP4 c-terminal labeled with mScarlet in mammalian cells

Depositor

Erik Dent

Article

Taylor et al Life Sci Alliance. 2019 Jun 3;2(3). pii:

Insert

Cdc42 Interacting Protein 4 (TRIP10 Human)

Expression

Mammalian

Promoter

CAG

Available Since

Jan. 11, 2022

Availability

Academic Institutions and Nonprofits only

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L_NLS 67kDa

Plasmid

#201361

Purpose

Nucleocytoplasmic transport probe

Depositor

Pere Roca-Cusachs

Article

Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

Insert

(NLS)SV40A4-EGFP-6PrA

Expression

Mammalian

Available Since

July 11, 2023

Availability

Academic Institutions and Nonprofits only

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H_NLS 67kDa

Plasmid

#201372

Purpose

Nucleocytoplasmic transport probe

Depositor

Pere Roca-Cusachs

Article

Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

Insert

(NLS)SV40-EGFP-6PrA

Expression

Mammalian

Available Since

July 11, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-EnhancedSynapsin-DIO-TVA950-EYFP-WPRE

Plasmid

#120269

Purpose

Allows for initial cellular transduction of EnvA-pseudotyped Rb via Cre-dependent expression of an avian TVA receptor fused with yellow fluorescent protein

Depositor

Edward Callaway

Article

Faget et al Cell Rep. 2016 Jun 21;15(12):2796-808. d

Insert

eSyn-DIO-TVA950:YFP

Use

AAV

Expression

Mammalian

Available Since

March 6, 2019

Availability

Academic Institutions and Nonprofits only

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pET27b-ecDHFR-N23PP/L28F

Plasmid

#118584

Purpose

Bacterial expression of E.coli DHFR N23PP and L28F mutant

Depositor

Stephen Benkovic

Article

Liu et al Proc Natl Acad Sci U S A. 2013 Jun 18;11

Insert

DHFR

Expression

Bacterial

Mutation

L28 mutated to F28, N23 mutated to P23, a proline…

Promoter

T7

Available Since

Nov. 8, 2018

Availability

Academic Institutions and Nonprofits only

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N118 nLV EF-1a-Gal-FKBPx1-HA-PGK-Blast

Plasmid

#44245

Depositor

Jerry Crabtree

Article

Hathaway et al Cell. 2012 Jun 22;149(7):1447-60. doi: 1

Insert

FKBP (FKBP1A Human)

Use

Lentiviral

Tags

Gal4/DBD/aa1-147 and HA

Promoter

EF-1a

Available Since

June 5, 2013

Availability

Academic Institutions and Nonprofits only

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3xFlag-HaloTag-EZH2 HDR - pDY053

Plasmid

#171108

Purpose

Homology directed repair plasmid for generating HaloTag-EZH2 cells

Depositor

Thomas Cech

Article

Youmans et al Genes Dev. 2018 Jun 1;32(11-12):794-805.

Insert

EZH2 (EZH2 Human)

Tags

HaloTag

Expression

Mammalian

Available Since

July 21, 2021

Availability

Academic Institutions and Nonprofits only

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SRSF8_RRM_pGEX

Plasmid

#135119

Purpose

Recombinant expression of SRSF8 with dual-affinity tag

Depositor

Christopher Burge

Article

Dominguez et al Mol Cell. 2018 Jun 7;70(5):854-867.e9. d

Insert

SRSF8 (SRSF8 Human)

Tags

GST-SBP

Expression

Bacterial

Promoter

tac

Available Since

Dec. 5, 2022

Availability

Academic Institutions and Nonprofits only

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PAK4-FL-3'UTR

Plasmid

#113088

Purpose

Luciferase reporter containing PAK4- FL-3'UTR

Depositor

James Donahue

Article

Phatak et al Oncotarget. 2018 Jun 19;9(47):28391-2840

Insert

p21 Activated kinase 4 (PAK4 Human)

Use

Luciferase

Expression

Mammalian

Mutation

none

Promoter

PGK

Available Since

Aug. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-DIO-hBFP-2A-RVG-WPRE

Plasmid

#120268

Purpose

Permits retrograde transsynaptic spread by driving Cre-dependent co-expression of the RbG plus a nuclear reporter

Depositor

Edward Callaway

Article

Faget et al Cell Rep. 2016 Jun 21;15(12):2796-808. d

Insert

EF1α-DIO-H2B-tagBFP-Flagx3-T2Am-cB19G

Use

AAV

Expression

Mammalian

Available Since

March 6, 2019

Availability

Academic Institutions and Nonprofits only

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pVC-Ds-ubb:MCP-p65-HSF1-2a-Cerulean-Ds

Plasmid

#119074

Purpose

Zebrafish ubiquitin promoter driving MCP-p65-HSF1 with Cerulean reporter

Depositor

Tatjana Sauka-Spengler

Article

Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

Insert

ubb:MCP-p65-HSF1-2a-Cerulean

Expression

Bacterial

Promoter

Zebrafish ubiquitin promoter

Available Since

Feb. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pVC-Ds-ubb:Com-KRAB-2a-Cerulean-Ds

Plasmid

#119075

Purpose

Zebrafish ubiquitin promoter driving Com-KRAB with Cerulean reporter

Depositor

Tatjana Sauka-Spengler

Article

Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

Insert

ubb:Com-KRAB-2a-Cerulean

Expression

Bacterial

Promoter

Zebrafish ubiquitin promoter

Available Since

Feb. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pVC-Ds-DrU6a:sgRNA-com-Ds

Plasmid

#119077

Purpose

To clone sgRNA spacer sequence for microinjections. This sgRNA tracrRNA contains 2x com stem loops

Depositor

Tatjana Sauka-Spengler

Article

Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

Insert

U6a:2xCom-sgRNA

Expression

Bacterial

Promoter

Zebrafish U6a promoter

Available Since

Feb. 6, 2019

Availability

Academic Institutions and Nonprofits only

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