We narrowed to 1,376 results for: piggyBac

1. PB_TRE_sg61-PDGFR_EF1a_eGFP

   Plasmid

   #186310

   Purpose

   Plasmid for inducible mammalian cell expression of the sg61 helixCAM and constitutive expression of an identifying fluorescent protein (eGFP). Flanked with PiggyBac sites for easy line construction

   Depositor

   George Church

   Article

   Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01

   Inserts

   sg61-PDGFR
   eGFP
   

   Tags

   His, myc

   Expression

   Mammalian

   Available Since

   Aug. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. PB_TRE_Z18-PDGFR_EF1a_eGFP

   Plasmid

   #186304

   Purpose

   Plasmid for inducible mammalian cell expression of the Z18 helixCAM and constitutive expression of an identifying fluorescent protein (eGFP). Flanked with PiggyBac sites for easy line construction.

   Depositor

   George Church

   Article

   Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01

   Inserts

   Z18-PDGFR
   eGFP
   

   Tags

   HA, myc

   Expression

   Mammalian

   Available Since

   Aug. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. PB_TRE_Z17-PDGFR_EF1a_mCherry

   Plasmid

   #186303

   Purpose

   Plasmid for inducible mammalian cell expression of the Z17 helixCAM and constitutive expression of an identifying fluorescent protein (mCherry). Flanked with PiggyBac sites for easy line construction.

   Depositor

   George Church

   Article

   Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01

   Inserts

   Z17-PDGFR
   mCherry
   

   Tags

   HA, myc

   Expression

   Mammalian

   Available Since

   Aug. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. vYM019_PB-Blast-pEF1a-H2B-mCh-D3bCD

   Plasmid

   #197687

   Purpose

   plasmid for expressing the DNMT3bCD

   Depositor

   Michael Elowitz

   Article

   Ma et al ACS Synth Biol. 2023 Aug 12. doi: 10.102

   Insert

   H2B-mCherry-T2A-rTetR-DNMT3bCD (DNMT3B Human, Synthetic)
   

   Use

   Synthetic Biology

   Expression

   Mammalian

   Available Since

   April 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. PB_TRE_AP4-PDGFR_EF1a_eBFP2

   Plasmid

   #186306

   Purpose

   Plasmid for inducible mammalian cell expression of the AP4 helixCAM and constitutive expression of an identifying fluorescent protein (eBFP2). Flanked with PiggyBac sites for easy line construction.

   Depositor

   George Church

   Article

   Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01

   Inserts

   AP4-PDGFR
   eBFP2
   

   Tags

   HA, myc

   Expression

   Mammalian

   Available Since

   Aug. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. PB_TRE_sg83-PDGFR_EF1a_iRFP670

   Plasmid

   #186311

   Purpose

   Plasmid for inducible mammalian cell expression of the sg83 helixCAM and constitutive expression of an identifying fluorescent protein (iRFP670). Flanked with PiggyBac sites for easy line construction

   Depositor

   George Church

   Article

   Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01

   Inserts

   sg83-PDGFR
   iRFP670
   

   Tags

   His, myc

   Expression

   Mammalian

   Available Since

   Aug. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. PB_TRE_sg88-PDGFR_EF1a_eBFP2

   Plasmid

   #186312

   Purpose

   Plasmid for inducible mammalian cell expression of the sg88 helixCAM and constitutive expression of an identifying fluorescent protein (eBFP2). Flanked with PiggyBac sites for easy line construction.

   Depositor

   George Church

   Article

   Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01

   Inserts

   sg88-PDGFR
   eBFP2
   

   Tags

   His, myc

   Expression

   Mammalian

   Available Since

   Aug. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. PB_TRE_P9-PDGFR_EF1a_mPlum

   Plasmid

   #186307

   Purpose

   Plasmid for inducible mammalian cell expression of the P9 helixCAM and constitutive expression of an identifying fluorescent protein (mPlum). Flanked with PiggyBac sites for easy line construction.

   Depositor

   George Church

   Article

   Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01

   Inserts

   P9-PDGFR
   mPlum
   

   Tags

   HA, myc

   Expression

   Mammalian

   Available Since

   Aug. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. PB_TRE_AP10-PDGFR_EF1a_mOrange

   Plasmid

   #186308

   Purpose

   Plasmid for inducible mammalian cell expression of the AP10 helixCAM and constitutive expression of an identifying fluorescent protein (mOrange). Flanked with PiggyBac sites for easy line construction

   Depositor

   George Church

   Article

   Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01

   Inserts

   AP10-PDGFR
   mOrange
   

   Tags

   HA, myc

   Expression

   Mammalian

   Available Since

   Aug. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. PB_TRE_P3-PDGFR_EF1a_iRFP670

    Plasmid

    #186305

    Purpose

    Plasmid for inducible mammalian cell expression of the P3 helixCAM and constitutive expression of an identifying fluorescent protein (iRFP670). Flanked with PiggyBac sites for easy line construction.

    Depositor

    George Church

    Article

    Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01

    Inserts

    P3-PDGFR
    iRFP670
    

    Tags

    HA, myc

    Expression

    Mammalian

    Available Since

    Aug. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pB-EF1a-NLuc-IRES-Puro

    Plasmid

    #130936

    Purpose

    PiggyBac vector for constitutive NanoLuc expression in mammalian cells

    Depositor

    James Thomson

    Article

    Chu et al Cell Rep. 2019 Aug 27;28(9):2247-2255.e5

    Insert

    NanoLuc
    

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Oct. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. PB-TO-NGN2-EMX1 BSD-mApple

    Plasmid

    #182311

    Purpose

    Piggybac Tet-ON plasmid for differentiating hiPSCs into glutamatergic neurons via NGN2 and EMX1 expression, bsd selection, mApple

    Depositor

    iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

    Inserts

    hNGN2-P2A-EMX1 (NEUROG2, EMX1 Human)
    bsd-T2A-mycNLS-mApple
    

    Tags

    T2A-mycNLS-mApple

    Expression

    Mammalian

    Promoter

    EF1a and TRE3G

    Available Since

    April 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pPB-EOS-GFP-IP

    Plasmid

    #60439

    Purpose

    PiggyBac for EOS-GFP

    Depositor

    Austin Smith

    Article

    Takashima et al Cell. 2014 Sep 11;158(6):1254-69. doi: 1

    Insert

    EOS
    

    Available Since

    Sept. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. PB-ReNL

    Plasmid

    #123138

    Purpose

    Piggybac transposon plasmid for live animal optical imaging of Red-enhanced nanolantern

    Depositor

    Jordan Green

    Article

    Karlsson et al Adv Funct Mater. 2021 Apr 22;31(17). doi

    Insert

    Red-enhanced Nanolantern
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Feb. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. nos-Cas9.874Z1

    Plasmid

    #112685

    Purpose

    Express hSpCas9 under nanos promoter

    Depositor

    Omar Akbari

    Article

    Kandul et al Nat Commun. 2019 Jan 8;10(1):84. doi: 10

    Insert

    hSpCas9
    

    Tags

    3x FLAG, EGFP, and NLS

    Expression

    Insect

    Mutation

    Humanized Cas9 bacterial sequence

    Promoter

    nanos

    Available Since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBS-PtH2B

    Plasmid

    #133390

    Purpose

    Piggybac vector for SRIRACCHA-mediated mutation enrichment (donor repair template for HDR)

    Depositor

    Blair Madison

    Article

    Wen et al J Biol Chem. 2017 Apr 14;292(15):6148-61

    Type

    Empty backbone

    Expression

    Mammalian

    Promoter

    No promoter

    Available Since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAC103-PBNeo-U6-sgRNA-Expression

    Plasmid

    #48228

    Purpose

    sgRNA expression on PiggyBac vector with Neo-selectable marker

    Depositor

    Rudolf Jaenisch

    Article

    Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    April 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. BII-CPspaH2B

    Plasmid

    #133385

    Purpose

    Piggybac vector for SRIRACCHA-mediated mutation enrichment (surrogate target for HDR)

    Depositor

    Blair Madison

    Article

    Wen et al J Biol Chem. 2017 Apr 14;292(15):6148-61

    Type

    Empty backbone

    Expression

    Mammalian

    Available Since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. NAX_CAGGS_CAS9_m4_KRAB-MeCP2_KanR neo

    Plasmid

    #167901

    Purpose

    PiggyBac compatible plasmid expressing dCas9-KRAB-MeCP2 expression plasmid

    Depositor

    Alejandro Chavez, Nan Cher Yeo

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Insert

    dCas9-KRAB-MeCP2
    

    Use

    CRISPR

    Mutation

    D10A, D839A, H840A, N863A

    Available Since

    Sept. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. KA0637_pPBCAG-rtTAM2-IN

    Plasmid

    #124166

    Purpose

    piggyBac-based expression of rtTA-M2

    Depositor

    Hans Schöler

    Article

    Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

    Insert

    rtTAM2-IRES-Neo
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    March 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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