We narrowed to 1,267 results for: piggyBac

1. pAS1_4x7SKPylT_EF1_Ub-*CoV2_9b

   Plasmid

   #162816

   Purpose

   STELLA plasmid for amber suppression of N-terminally labelled CoV2_9b

   Depositor

   Simon Elsaesser

   Article

   Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

   Insert

   Ub-(N-TAG)CoV2_9b
   

   Use

   Piggybac

   Tags

   Expression

   Mammalian

   Mutation

   CoV2_9b(Met1,Amber)

   Promoter

   EF1alpha

   Available since

   Dec. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAS1_4x7SKPylT_EF1_Ub-*CoV2_ORF6

   Plasmid

   #162817

   Purpose

   STELLA plasmid for amber suppression of N-terminally labelled CoV2_ORF6

   Depositor

   Simon Elsaesser

   Article

   Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

   Insert

   Ub-(N-TAG)CoV2_ORF6
   

   Use

   Piggybac

   Tags

   Expression

   Mammalian

   Mutation

   CoV2_ORF6(Met1,Amber)

   Promoter

   EF1alpha

   Available since

   Dec. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAS1_4x7SKPylT_EF1_Ub-*CoV2_ORF7b

   Plasmid

   #162818

   Purpose

   STELLA plasmid for amber suppression of N-terminally labelled CoV2_ORF7b

   Depositor

   Simon Elsaesser

   Article

   Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

   Insert

   Ub-(N-TAG)CoV2_ORF7b
   

   Use

   Piggybac

   Tags

   Expression

   Mammalian

   Mutation

   CoV2_ORF7b(Met1,Amber)

   Promoter

   EF1alpha

   Available since

   Dec. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAS1_4x7SKPylT_EF1_Ub-*CoV2_ORF10

   Plasmid

   #162819

   Purpose

   STELLA plasmid for amber suppression of N-terminally labelled CoV2_ORF10

   Depositor

   Simon Elsaesser

   Article

   Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

   Insert

   Ub-(N-TAG)CoV2_ORF10
   

   Use

   Piggybac

   Tags

   Expression

   Mammalian

   Mutation

   CoV2_ORF10(Met1,Amber)

   Promoter

   EF1alpha

   Available since

   Dec. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAS1_4x7SKPylT_EF1_Ub-*CoV2_ORF3b_alt

   Plasmid

   #162821

   Purpose

   STELLA plasmid for amber suppression of N-terminally labelled CoV2_ORF3b_alt

   Depositor

   Simon Elsaesser

   Article

   Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

   Insert

   Ub-(N-TAG)CoV2_ORF3b_alt
   

   Use

   Piggybac

   Tags

   Expression

   Mammalian

   Mutation

   CoV2_ORF3b_alt(Met1,Amber)

   Promoter

   EF1alpha

   Available since

   Dec. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. BII-C3H

   Plasmid

   #133386

   Purpose

   Piggybac vector for SRIRACCHA-mediated mutation enrichment (surrogate target for HDR)

   Depositor

   Blair Madison

   Article

   Wen et al J Biol Chem. 2017 Apr 14;292(15):6148-61

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. BII-Sh-PnGW-CMV

   Plasmid

   #133366

   Purpose

   Piggybac vector for shRNA-mediated knockdown, co-expressed with GFP-NLS

   Depositor

   Blair Madison

   Article

   Fischer et al Am J Physiol Gastrointest Liver Physiol.

   Insert

   GFP-NLS
   

   Use

   Tags

   HA-tagged GFP

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   CMV

   Available since

   Jan. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. BII-B3HTK

   Plasmid

   #133389

   Purpose

   Piggybac vector for SRIRACCHA-mediated mutation enrichment (surrogate target for HDR)

   Depositor

   Blair Madison

   Article

   Wen et al J Biol Chem. 2017 Apr 14;292(15):6148-61

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. BII-ChPtW-eSpCas9

   Plasmid

   #133381

   Purpose

   Piggybac vector for constitutive expression

   Depositor

   Blair Madison

   Article

   Fischer et al Am J Physiol Gastrointest Liver Physiol.

   Insert

   eSpCas9
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   eSpCas9 mutations

   Promoter

   CMV enhancer and hEf1a (CpG free)

   Available since

   Jan. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. BII-Sh-PnGW-CMV-Luc

    Plasmid

    #133367

    Purpose

    Piggybac vector for shRNA-mediated knockdown, co-expressed with GFP-NLS

    Depositor

    Blair Madison

    Article

    Fischer et al Am J Physiol Gastrointest Liver Physiol.

    Insert

    GFP-NLS
    

    Use

    Tags

    HA-tagged GFP

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    CMV

    Available since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. BII-Sh-PIR702W-Luc

    Plasmid

    #133362

    Purpose

    Piggybac vector for shRNA-mediated knockdown, co-expressed with GFP-NLS

    Depositor

    Blair Madison

    Article

    Fischer et al Am J Physiol Gastrointest Liver Physiol.

    Insert

    IRFP-702
    

    Use

    Tags

    HA-tagged IRFP

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    CMV enhancer and hEf1a (CpG free)

    Available since

    Jan. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. BII-Sh-PIR702W

    Plasmid

    #133361

    Purpose

    Piggybac vector for shRNA-mediated knockdown, co-expressed with GFP-NLS

    Depositor

    Blair Madison

    Article

    Fischer et al Am J Physiol Gastrointest Liver Physiol.

    Insert

    IRFP-702
    

    Use

    Tags

    HA-tagged IRFP

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    CMV enhancer and hEf1a (CpG free)

    Available since

    Jan. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. BII-Sh-PnGW-Luc

    Plasmid

    #133360

    Purpose

    Piggybac vector for shRNA-mediated knockdown, co-expressed with GFP-NLS

    Depositor

    Blair Madison

    Article

    Fischer et al Am J Physiol Gastrointest Liver Physiol.

    Insert

    GFP-NLS
    

    Use

    Tags

    HA-tagged GFP

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    CMV enhancer and hEf1a (CpG free)

    Available since

    Jan. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. KA1063_pPB-hCMV*1-cHA-IRESH2BmKO2

    Plasmid

    #124169

    Purpose

    piggyBac-based Dox-inducible expression

    Depositor

    Hans Schöler

    Article

    Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. KA2896_pPB-TREtight-Tkmin-cHA-IRESH2BmKO2

    Plasmid

    #124175

    Purpose

    piggyBac-based Dox-inducible expression (very weak)

    Depositor

    Hans Schöler

    Article

    Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. KA2895_pPB-TREtight-TATAmin-cHA-IRESH2BmKO2

    Plasmid

    #124174

    Purpose

    piggyBac-based Dox-inducible expression (weak)

    Depositor

    Hans Schöler

    Article

    Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. KA1123_pPB-TREtight-cHA-IRESH2BmKO2

    Plasmid

    #124172

    Purpose

    piggyBac-based Dox-inducible expression

    Depositor

    Hans Schöler

    Article

    Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pSLQ2840 pPB: CAG-Puro-WPRE PGK-VPR-tagBFP-SadCas9

    Plasmid

    #84246

    Purpose

    Expresses direct fusion VPR-Sa dCas9

    Depositor

    Stanley Qi

    Article

    Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

    Inserts

    VPR-tagBFP-Sa dCas9
    Puro
    

    Use

    CRISPR and Synthetic Biology; Piggybac

    Tags

    Nucleoplasmin NLS, SV40 NLS, VPR, and tagBFP

    Expression

    Mammalian

    Mutation

    Promoter

    CAG and PGK

    Available since

    Nov. 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pJAM1.21_PB-UGCG-PGK-Blast

    Plasmid

    #205566

    Purpose

    Express human UGCG

    Depositor

    Michael Elowitz

    Article

    Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

    Insert

    UGCG (UGCG Human)
    

    Use

    Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    EF1

    Available since

    Sept. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pFH2.105_PB-EPN24-MCP-T2A-eGFP-PGK-Blast

    Plasmid

    #205550

    Purpose

    Express EPN24-MCP-T2A-eGFP and blasticidin resistance marker

    Depositor

    Michael Elowitz

    Article

    Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

    Insert

    EPN24-MCP
    

    Use

    Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    EF1

    Available since

    Sept. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge