We narrowed to 12,873 results for: sequence

1. ZipGFP11_TEV

   Plasmid

   #81243

   Purpose

   Zipped GFP11 with TEV cleavage sequence

   Depositor

   Xiaokun Shu

   Article

   To et al Cell Chem Biol. 2016 Jul 21;23(7):875-82

   Insert

   zipped GFP11 with TEV cleavage sequence
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 18, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. SpCas9-CBE6a-IVT

   Plasmid

   #215833

   Purpose

   Template for in vitro transcription of CBE6a (with SpCas9)

   Depositor

   David Liu

   Article

   Zhang et al Nat Commun. 2024 Feb 24;15(1):1697. doi:

   Insert

   CBE6a-SpCas9-2xUGI
   

   Use

   In vitro transcription template

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. ERoxBFP

   Plasmid

   #68126

   Purpose

   mammalian expression of ER localized oxBFP

   Depositor

   Erik Snapp

   Article

   Costantini et al Nat Commun. 2015 Jul 9;6:7670. doi: 10.1

   Insert

   oxBFP
   

   Use

   Tags

   KDEL ER retention sequence and bovine prolactin s…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

4. LbCas12a-gRNA_BbsI_CAM

   Plasmid

   #183222

   Purpose

   LbCas12a gRNA containing BbsI restriction recognition sites in spacer sequence for Golden Gate Assembly

   Depositor

   Jennifer Doudna

   Article

   Huang et al Mol Cell. 2022 Jun 2;82(11):2148-2160.e4

   Insert

   LbCas12a guide RNA containing BbsI sites in spacer sequence
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pKIKOrbsARKm

   Plasmid

   #46768

   Purpose

   E.coli genomic integration rbsAR locus

   Depositor

   Lars Nielsen, Claudia Vickers

   Article

   Sabri et al Microb Cell Fact. 2013 Jun 24;12(1):60.

   Type

   Empty backbone

   Use

   E.coli genomic integration

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 24, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSJ111

   Plasmid

   #78707

   Purpose

   Modular vector for constitutive secreted amylase reporter expression

   Depositor

   Wiep Klaas Smits

   Article

   Oliveira Paiva et al ACS Synth Biol. 2016 Jun 23.

   Insert

   amyEopt
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Pveg

   Available since

   Sept. 30, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. 469_pME_iCre

   Plasmid

   #171792

   Purpose

   pME-Vector with codon improved iCre sequence. Tol2-kit compatible.

   Depositor

   Jean Giacomotto

   Article

   Tromp et al Front Physiol. 2023 Aug 2;14:1221310. do

   Insert

   codon-optimized iCre sequence
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   None

   Available since

   Jan. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMito-TrxRFP1

   Plasmid

   #98997

   Purpose

   mammalian expression of a biosensor to monitor thioredoxin redox dynamics, mitochondrially locaclized

   Depositor

   Huiwang Ai

   Article

   Fan et al Nat Chem Biol. 2017 Jun 26. doi: 10.1038

   Insert

   TrxRFP1 and mitochondrial localization sequence
   

   Use

   Tags

   His-tag and Mitochondria localization sequence

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMAH-ER-bapaUGAc

   Plasmid

   #201674

   Purpose

   boronic acid- and peptide-assisted UDP-GlcNAc sensor in ER

   Depositor

   Huiwang Ai

   Article

   Zhang et al ACS Sens. 2023 Jul 22. doi: 10.1021/acss

   Insert

   ER-bapaUGAc
   

   Use

   Tags

   ER leading sequence and ER retention sequence

   Expression

   Mammalian

   Mutation

   Promoter

   CMV SP6

   Available since

   Aug. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. ptRNAfMet-A37

    Plasmid

    #196657

    Purpose

    Plasmid for overexpression of native initiator tRNA (A37)

    Depositor

    Markus Jeschek

    Article

    Hollerer et al Nucleic Acids Res. 2023 Mar 21;51(5):237

    Insert

    tRNAfMet-A37 expression cassette
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    metY native promoter

    Available since

    March 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRC319

    Plasmid

    #61272

    Purpose

    Also referred to as pΦRGNndm-1. Expresses Cas9, tracrRNA and a guide RNA targeting the NDM-1 gene. Contains a ColE1 origin, kanamycin resistance cassette and f1 origin for packaging in M13 particles.

    Depositor

    Timothy Lu

    Article

    Citorik et al Nat Biotechnol. 2014 Sep 21. doi: 10.103

    Insert

    Cas9, tracrRNA, crRNA
    

    Use

    CRISPR

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. p38delta

    Plasmid

    #29575

    Depositor

    Dustin Maly

    Article

    Hari et al Chem Biol. 2013 Jun 20;20(6):806-15. doi

    Insert

    p38delta (Mapk13 Mouse)
    

    Use

    Tags

    HIS6 and TEV

    Expression

    Bacterial

    Mutation

    Starting Met replaced with Ala

    Promoter

    Available since

    July 13, 2011

    Availability

    Academic Institutions and Nonprofits only

13. PE-Cas9

    Plasmid

    #174851

    Purpose

    Express PE-Cas9 in mammalian cells

    Depositor

    Wen Xue

    Article

    Jiang et al Nat Biotechnol. 2021 Oct 14. pii: 10.103

    Insert

    PE-Cas9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGZ136 (pRS406-pREB1-3FLAG-MNase-NLS)

    Plasmid

    #72273

    Purpose

    FLAG-Mnase expressed under the REB1 promoter

    Depositor

    Steven Henikoff

    Article

    Zentner et al Nat Commun. 2015 Oct 22;6:8733. doi: 10.

    Insert

    3xFLAG tag and aa 83-231 of Mnase
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    REB1

    Available since

    Jan. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

15. pQA-LP574

    Plasmid

    #222953

    Purpose

    Expresses R120A LoaP

    Depositor

    Adrian Ferré-D'Amaré

    Article

    Elghondakly et al Structure. 2024 Jun 28:S0969-2126(24)002

    Insert

    antiterminator protein LoaP
    

    Use

    Tags

    10xHIS-SUMO

    Expression

    Bacterial

    Mutation

    mutated Arginine 120 to Alanine

    Promoter

    T7

    Available since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. p153 pPr-cre

    Plasmid

    #8403

    Purpose

    Expression of Cre from tissue-specific, androgen-inducible probasin promoter.

    Depositor

    Jeffrey Green

    Article

    Kaczmarczyk et al Nucleic Acids Res 2001 Jun 15;29(12):E56

    Insert

    probasin driven Cre
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    probasin promoter

    Available since

    April 28, 2006

    Availability

    Academic Institutions and Nonprofits only

17. mOrange pHis-//

    Plasmid

    #53304

    Purpose

    Empty vector to express N-terminal His-mOrange-tagged fusion protein.

    Depositor

    John Lis

    Article

    Tome et al Nat Methods. 2014 Jun;11(6):683-8. doi:

    Type

    Empty backbone

    Use

    Tags

    His and mOrange

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. SAP145

    Plasmid

    #11239

    Depositor

    Robin Reed

    Article

    Gozani et al Genes Dev. 1996 Jan 15. 10(2):233-43.

    Insert

    SAP145 (SF3B2 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 19, 2006

    Availability

    Academic Institutions and Nonprofits only

19. Tus pGST-//

    Plasmid

    #53305

    Purpose

    Expresses GST-tagged E.coli replication terminator protein Tus.

    Depositor

    John Lis

    Article

    Tome et al Nat Methods. 2014 Jun;11(6):683-8. doi:

    Insert

    E.Coli Tus replication terminator protein
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. eNme2C-CBE6a

    Plasmid

    #215829

    Purpose

    Express CBE6a (with eNme2-C variant) in mammalian cells

    Depositor

    David Liu

    Article

    Zhang et al Nat Commun. 2024 Feb 24;15(1):1697. doi:

    Insert

    CBE6a-eNme2C-2xUGI
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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