We narrowed to 21,881 results for: tes

1. pLV_hU6-sgRNA_hUbC-GFP-P2A-PuroR

   Plasmid

   #162335

   Purpose

   Lentiviral expression of S. pyogenes sgRNA with a GFP-P2A-PuroR selection marker

   Depositor

   Charles Gersbach

   Article

   Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi:

   Insert

   S. pyogenes sgRNA
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   Feb. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pZDonor-Nm-Cas9-gRNA-hU6

   Plasmid

   #61366

   Purpose

   empty backbone for cloning N. meningiditis protospacers. Encodes constant region of N. meningiditis Cas9 synthetic gRNA

   Depositor

   Charles Gersbach

   Article

   Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   human U6

   Available since

   April 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. mVenusC-Cavin3

   Plasmid

   #108890

   Purpose

   Expression of Cavin3 fused to the C-terminus of mVenus for bimolecular fluorescence complementation

   Depositor

   Rob Parton

   Article

   Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

   Insert

   Cavin3 (altered codon usage) (CAVIN3 Synthetic, Human)
   

   Use

   Tags

   mVenus156-239

   Expression

   Mammalian

   Mutation

   altered codon usage

   Promoter

   Available since

   March 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. actc1b-mKate2-ING2

   Plasmid

   #109502

   Purpose

   Muscle specific zebrafish expression of mKate2 fused to 3xPHD domain from Mouse inhibitor of growth family member 2 (ING2), codon optimised. Binds PtdIns5P

   Depositor

   Rob Parton

   Article

   Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

   Insert

   actc1b-mKate2-ING2
   

   Use

   Zebrafish plasmids

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. p3E-ML1N*2

   Plasmid

   #67678

   Purpose

   Multisite gateway entry clone for adding C-terminal fusions of ML1N*2

   Depositor

   Rob Parton

   Article

   Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

   Insert

   ML1N*2
   

   Use

   Gateway multisite 3' entry clone

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA5-eYFP-FKBP-MAD1-WT

   Plasmid

   #114037

   Purpose

   Chemical induction of MAD1 localisation to a specific site in the cell (by rapamycin & FRB)

   Depositor

   Geert Kops

   Article

   Kuijt et al Chromosoma. 2014 Oct;123(5):471-80. doi:

   Insert

   Human MAD1 (MAD1L1 Human)
   

   Use

   Tags

   eYFP-FKBP12

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV

   Available since

   Oct. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. Bact2-APEX2-GBP in pDEST-Tol2-pA2-acrys-cherry

   Plasmid

   #67668

   Purpose

   Zebrafish expression vector for ubiquitous expression of APEX2-GBP

   Depositor

   Rob Parton

   Article

   Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

   Insert

   APEX2-GBP
   

   Use

   Zebrafish expression with tol2

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. mVenusN-Cavin3

   Plasmid

   #108889

   Purpose

   Expression of Cavin3 fused to the N-terminus of mVenus for bimolecular fluorescence complementation

   Depositor

   Rob Parton

   Article

   Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

   Insert

   Cavin3 (altered codon usage) (CAVIN3 Synthetic, Human)
   

   Use

   Tags

   mVenus1-155

   Expression

   Mammalian

   Mutation

   altered codon usage

   Promoter

   Available since

   March 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. p3E-RhoAb-DN

   Plasmid

   #109580

   Purpose

   Multisite gateway vector for 3' tagging with dominant negative RhoAb

   Depositor

   Rob Parton

   Article

   Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

   Insert

   RhoAb (rhoab Zebrafish)
   

   Use

   Zebrafish plasmids

   Tags

   Expression

   Mutation

   dominant negative

   Promoter

   Available since

   Feb. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. p3E-APEX2-P2A-mKate2

    Plasmid

    #67671

    Purpose

    Multisite gateway entry clone for adding C-terminal fusions of APEX2-P2A-mKate2

    Depositor

    Rob Parton

    Article

    Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

    Insert

    APEX2-P2A-mKate2
    

    Use

    Gateway multisite 3' entry clone

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJB332

    Plasmid

    #115949

    Purpose

    ORF insert for replicon MoClo

    Depositor

    Ron Weiss

    Article

    Wagner et al Nat Chem Biol. 2018 Nov;14(11):1043-1050

    Insert

    Lvl0 TetR-Gly9-DDX6 (contains SapI)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTW122

    Plasmid

    #115962

    Purpose

    ORF insert for replicon MoClo

    Depositor

    Ron Weiss

    Article

    Wagner et al Nat Chem Biol. 2018 Nov;14(11):1043-1050

    Insert

    Lvl0 5'-2xTetApt-mVenus-PEST
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. p3E-ING2

    Plasmid

    #109554

    Purpose

    Multisite gateway vector for 3' tagging with 3xPHD domain from Mouse inhibitor of growth family member 2 (ING2), codon optimised. Binds PtdIns5P

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    3xPHD domain from Mouse inhibitor of growth family member 2 (ING2), codon optimised
    

    Use

    Zebrafish plasmids

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAPcrRNA_Lmo

    Plasmid

    #126501

    Purpose

    L.monocytogenes CRISPR Type I-B crRNA cloning vector for mammalian expression driven by U6 promoter

    Depositor

    Charles Gersbach

    Article

    Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Sept. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. Hyper7 in pT3TS-Dest

    Plasmid

    #194296

    Purpose

    In vitro transcription of the hydrogen peroxide sensor Hyper7 from the T3 promoter. Parton lab clone KRL

    Depositor

    Rob Parton

    Article

    Wu et al Dev Cell. 2023 Mar 13;58(5):376-397.e4.

    Insert

    Hyper7
    

    Use

    In vitro transcription of mrna

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLKO-puro human lipin 1-1 shRNA

    Plasmid

    #32019

    Depositor

    David Sabatini

    Article

    Peterson et al Cell. 2011 Aug 5;146(3):408-20.

    Insert

    lipin1 shRNA
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Sept. 27, 2011

    Availability

    Academic Institutions and Nonprofits only

17. pXPR_003 Intergenic control guide 2

    Plasmid

    #193585

    Purpose

    sgRNA control; induces CAS9 cutting in an intergenic region

    Depositor

    William Hahn

    Article

    Hagel et al Cancer Res. 2022 Dec 22:CAN-22-2245. doi

    Insert

    sgRNA control
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pXPR_003 sgNFkB2 guide 2

    Plasmid

    #193594

    Purpose

    NFkB2 knockout

    Depositor

    William Hahn

    Article

    Hagel et al Cancer Res. 2022 Dec 22:CAN-22-2245. doi

    Insert

    sgNFkB2 guide 2 (NFKB2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pME-mKate-P2A-APEX2-NS

    Plasmid

    #67670

    Purpose

    Gateway entry clone containing mKate2-P2A-APEX2 with no stop codon

    Depositor

    Rob Parton

    Article

    Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

    Insert

    mKate2-P2A-APEX2-NS
    

    Use

    Gateway multisite middle entry clone

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. DDX5 D248N V5

    Plasmid

    #199577

    Purpose

    Expresses helicase dead DDX5 cloned into pLenti puro

    Depositor

    Michael Kastan

    Article

    McCann et al Radiat Res. 2023 Apr 1;199(4):406-421. d

    Insert

    DDX5 (DDX5 Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    D248N

    Promoter

    CMV

    Available since

    April 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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