161,261 results

1. pEGFP-ATF6

   Plasmid

   #32955

   Purpose

   For visualization of GFP-ATF6 in mammalian cells.

   Depositor

   Ron Prywes

   Article

   Chen et al J Biol Chem. 2002 Apr 12. 277(15):13045-

   Insert

   ATF6 (ATF6 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   M67L and M313I

   Promoter

   CMV

   Available since

   Nov. 7, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGreenII 0000 (No LUC)

   Plasmid

   #44465

   Depositor

   Roger Hellens

   Type

   Empty backbone

   Use

   Plant transformation

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 6, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. PH-PLCD1(R40L)-GFP

   Plasmid

   #51408

   Purpose

   Non-PI(4,5)P2 binding control for PH-PLCD1-GFP

   Depositor

   Tamas Balla

   Article

   Varnai et al J Cell Biol. 1998 Oct 19;143(2):501-10.

   Insert

   PH-PLCD1 (aa 1–170) (PLCD1 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Arginine 40 mutated to leucine

   Promoter

   Available since

   March 31, 2014

   Availability

   Academic Institutions and Nonprofits only

4. Lamin A-mRFP

   Plasmid

   #124268

   Purpose

   Wild-type Lamin A, C-terminally tagged with mRFP

   Depositor

   Eric Schirmer

   Insert

   LMNA (LMNA Human)
   

   Use

   Tags

   mRFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCI HA NEDD4

   Plasmid

   #27002

   Depositor

   Joan Massague

   Article

   Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

   Insert

   NEDD4 (NEDD4 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 11, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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6. pYPQ202

   Plasmid

   #86198

   Purpose

   Multisite Gateway T-DNA entry plasmid (attR1 & attR2); AtUBQ10 promoter and Hygromiycin resistance marker

   Depositor

   Yiping Qi

   Article

   Tang et al Nat Plants. 2017 Feb 17;3:17018. doi: 10

   Type

   Empty backbone

   Use

   Gateway compatible attr1-attr2 destination vector

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Arabidopsis ubiquitin 10 (AtUbi10)

   Available since

   May 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pPMQAK1-Ptrc-[E*](104)-Cas9-B0015-lacZ-PnrsB-mazF-LVA

   Plasmid

   #190790

   Purpose

   [E*](104) construct. Theophylline inducible Cas9 (S. pyogenes) expression. Two BsaI-sites allow for simultaneous insertion of sgRNA and donor DNA. Includes a nickel-inducible curing system.

   Depositor

   Paul Hudson

   Article

   Cengic et al ACS Synth Biol. 2022 Aug 15. doi: 10.102

   Inserts

   Cas9
   mazF-LVA
   

   Use

   CRISPR and Synthetic Biology

   Tags

   LVA

   Expression

   Bacterial

   Mutation

   silent mutations of BpiI-sites in original Cas9 g…

   Promoter

   PnrsB and Ptrc-[E*](104)

   Available since

   Feb. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRK5-myc-Miro2 T18N

   Plasmid

   #47897

   Purpose

   Expresses myc tagged Miro2 T18N dominant negative mutant

   Depositor

   Pontus Aspenström

   Article

   Fransson et al Biochem Biophys Res Commun. 2006 Jun 2;3

   Insert

   Miro2 T18N (RHOT2 Human)
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   T18N, dominant negative mutant

   Promoter

   CMV

   Available since

   Sept. 12, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. GFP-JIP4

   Plasmid

   #164620

   Purpose

   Expresses JIP4 in mammalian cells with a GFP tag

   Depositor

   Mark Cookson

   Article

   Bonet-Ponce et al Sci Adv. 2020 Nov 11;6(46). pii: 6/46/ea

   Insert

   JIP4 (SPAG9 Human)
   

   Use

   Tags

   cycle 3 GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pClathrin-miRFP670nano3

    Plasmid

    #184667

    Purpose

    Clathrin labelled with near-infrared fluorescent protein miRFP670nano3

    Depositor

    Vladislav Verkhusha

    Article

    Oliinyk et al Nat Methods. 2022 May 23. pii: 10.1038/s

    Insert

    miRFP670nano3-clathrin light chain
    

    Use

    Tags

    miRFP670nano3

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV-BE1

    Plasmid

    #73019

    Purpose

    Expresses BE1 with C-terminal NLS in mammalian cells

    Depositor

    David Liu

    Article

    Komor et al Nature. 2016 Apr 20. doi: 10.1038/nature

    Insert

    BE1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. GFP-p38alpha

    Plasmid

    #86832

    Purpose

    mammalian expression of GFP-p38alpha

    Depositor

    Rony Seger

    Article

    Zehorai et al Mol Cell Biol. 2014 Jan;34(2):259-70. do

    Insert

    p38alpha (MAPK14 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMuLE_EXPR_CMV-eGFP_TOP-iRFP_PTCH1-mTurquoise2_CBF-tdTomato

    Plasmid

    #113865

    Purpose

    Triple pathway reporter, 3P-Fluor; wnt-iRFP, hedgehog-mTurquoise2, notch-tdTomato; plus CMV-eGFP. Gateway expression vector for lentivirus generation.

    Depositor

    Manfred Ogris

    Article

    Maier et al PLoS One. 2019 Dec 20;14(12):e0226570. d

    Inserts

    CMV-eGFP
    TOP-iRFP
    PTCH1-mTurquoise2
    CBF-tdTomato
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. PCR4-Shh::lacZ-H11

    Plasmid

    #139098

    Purpose

    enSERT LacZ reporter vector for site-specific integration into the H11 locus (contains Shh minimal promoter)

    Depositor

    Len Pennacchio

    Article

    Kvon et al Cell. 2020 Mar 6. pii: S0092-8674(20)302

    Type

    Empty backbone

    Use

    CRISPR and Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Shh

    Available since

    March 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pWXC078

    Plasmid

    #222961

    Purpose

    C. elegans codon-optimized mScarlet-I3 with 3 introns cloned into pPD95.75 backbone

    Depositor

    Oliver Hobert

    Article

    Cao et al Genetics. 2024 Oct 7;228(2):iyae126. doi

    Insert

    mScarlet-I3
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    July 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRK5-myc-Miro2 A13V

    Plasmid

    #47896

    Purpose

    Expresses myc tagged Miro2 A13V constitutively active mutant

    Depositor

    Pontus Aspenström

    Article

    Fransson et al Biochem Biophys Res Commun. 2006 Jun 2;3

    Insert

    Miro2 A13V (RHOT2 Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    A13V, constitutively active mutant

    Promoter

    CMV

    Available since

    Sept. 12, 2013

    Availability

    Academic Institutions and Nonprofits only

17. pCM189-ParB1::mCherry

    Plasmid

    #87253

    Purpose

    Expresses ParB1-mCherry in yeast. Also known as pOR1-mch

    Depositor

    Kerstin Bystricky

    Article

    Saad et al PLoS Genet. 2014 Mar 13;10(3):e1004187.

    Insert

    ParB1
    

    Use

    Tags

    mCherry

    Expression

    Yeast

    Mutation

    Promoter

    pBAD/tetO

    Available since

    Aug. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. AAVS1-A2UCOE-EF1a-iCasp9-P2A-Crry-T2A-mCD59-E2A-Qa1 SCT-F2A-neo-AAVS1

    Plasmid

    #205445

    Purpose

    human AAVS1-targeting plasmid for expression of iCasp9, Crry, mouse CD59, and Qa1 SCT

    Depositor

    Deepta Bhattacharya

    Article

    Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

    Insert

    iCasp9-Crry-mCD59-Qa1 SCT
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. miRFP670nano-pcDNA

    Plasmid

    #127443

    Purpose

    Monomeric near-infrared fluorescent protein miRFP670nano in mammalian plasmid

    Depositor

    Vladislav Verkhusha

    Article

    Oliinyk et al Nat Commun. 2019 Jan 17;10(1):279. doi:

    Insert

    miRFP670nano
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. DRD2-Tango

    Plasmid

    #66269

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    DRD2 (DRD2 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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