19,660 results

1. pmCherry-ATG5-K130R

   Plasmid

   #13096

   Depositor

   Roberta Gottlieb

   Article

   Hamacher-Brady et al Cell Death Differ. 2007 Jan;14(1):146-57

   Insert

   ATG5-K130R (Atg5 Mouse)
   

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   K130R

   Available Since

   Nov. 15, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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2. VHL-pGex2TK

   Plasmid

   #20790

   Depositor

   William Kaelin

   Article

   Iliopoulos et al Nat Med. 1995 Aug . 1(8):822-6.

   Insert

   VHL (VHL Human)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   VHL wt cDNA, no mutations

   Available Since

   April 6, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSUMO1

   Plasmid

   #52258

   Purpose

   Bacterial expression of human 6His-tagged SUMO1, SUMO-E1 and SUMO-E2

   Depositor

   Primo Schaer

   Article

   Weber et al PLoS One. 2014 Jul 9;9(7):e102157. doi:

   Inserts

   SUMO-1 (SUMO1 Human)
   UBE2l (UBE2I Human)
   SAE-2 (UBA2 )
   SAE-1 (SAE1 Human)
   

   Tags

   6His-tag

   Expression

   Bacterial

   Mutation

   C-terminal gly-gly

   Promoter

   T7 and rbs

   Available Since

   July 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMJ117

   Plasmid

   #85997

   Purpose

   One-guide Perturb-seq vector backbone; modified human U6 promoter; constant region 3

   Depositor

   Jonathan Weissman

   Article

   Adamson et al Cell. 2016 Dec 15;167(7):1867-1882.e21.

   Inserts

   EGFP-NT2_cr3
   puro-T2A-BFP
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   modified human U6

   Available Since

   Feb. 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV-FLuc

   Plasmid

   #170575

   Purpose

   Express firefly luciferase. Used in MLV-based SARS-CoV-2 pseudovirus assay.

   Depositor

   David Nemazee

   Article

   Rogers et al Science. 2020 Aug 21;369(6506):956-963.

   Insert

   Luc
   

   Use

   Luciferase and Retroviral

   Tags

   NA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pORANGE GFP-Grin2a KI

   Plasmid

   #131486

   Purpose

   Endogenous tagging of GluN2a: N-terminal (amino acid position: A25)

   Depositor

   Harold MacGillavry

   Article

   Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

   Insert

   gRNA and GFP donor (Grin2a Rat)
   

   Expression

   Mammalian

   Promoter

   U6 and Cbh

   Available Since

   Sept. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. SOST/pcDNA3.1+

   Plasmid

   #10842

   Depositor

   Xi He

   Article

   Semenov et al J Biol Chem. 2005 Jul 22. 280(29):26770-

   Insert

   SOST (SOST Human)
   

   Expression

   Mammalian

   Available Since

   Aug. 4, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET246-pUC57 12xMS2V6

   Plasmid

   #104390

   Purpose

   MS2V6 cloning

   Depositor

   Robert Singer, Evelina Tutucci

   Article

   Tutucci et al Nat Methods. 2017 Nov 13. doi: 10.1038/n

   Insert

   12xMS2V6
   

   Expression

   Bacterial

   Mutation

   All Loops are U-variants, interspaced by 50 nucle…

   Available Since

   Dec. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRN111

   Plasmid

   #84463

   Purpose

   pJB38-NWMN29-30 + SarA_P1-mCherry-Term

   Depositor

   Reindert Nijland

   Article

   de Jong et al Sci Rep. 2017 Mar 7;7:43889. doi: 10.103

   Insert

   SarA_P1-mCherry from pRN11
   

   Available Since

   Feb. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. G15-CASE

    Plasmid

    #168126

    Purpose

    Encodes a BRET-based activity sensor for the heterotrimeric G protein G15. Composed of the subunits G alpha 15 (GNA15) tagged with NanoLuciferase, G beta 3 (GNB3) and Venus-tagged G gamma 9 (GNG9).

    Depositor

    Gunnar Schulte

    Article

    Schihada et al Sci Signal. 2021 Sep 7;14(699):eabf1653.

    Insert

    GNA15, GNB3, GNG9 (GNA15, GNB3, GNGT2 Human)
    

    Use

    Luciferase

    Tags

    cpVenus on GNG9

    Expression

    Mammalian

    Mutation

    NLuc is inserted at E244/N245 within GNA15

    Available Since

    July 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. NUMB2-eGFP-M107K

    Plasmid

    #37803

    Depositor

    Rolf Bjerkvig

    Article

    Euskirchen et al Exp Cell Res. 2011 Dec 10;317(20):2864-7

    Insert

    NUMB isoform 2 (NUMB Human)
    

    Use

    Lentiviral

    Tags

    eGFP

    Promoter

    pCMV

    Available Since

    March 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. mApple-pBAD

    Plasmid

    #54536

    Purpose

    Localization: Protein Expression Vector , Excitation: 568, Emission: 592

    Depositor

    Michael Davidson, Nathan Shaner, Roger Tsien

    Article

    Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

    Type

    Empty backbone

    Tags

    6xHis and mApple

    Expression

    Bacterial

    Available Since

    July 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET21a-ValRS-His

    Plasmid

    #124122

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Sebastian Maerkl, Takuya Ueda

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Valyl-tRNA ligase (valS Escherichia coli)
    

    Tags

    6x-His

    Expression

    Bacterial

    Available Since

    April 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3-ALK5 K232R

    Plasmid

    #80878

    Purpose

    mammalian expression of ALK5 K232R

    Depositor

    Aristidis Moustakas

    Article

    Moren et al J Biol Chem. 2005 Jun 10;280(23):22115-2

    Insert

    ALK5 (TGFBR1 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    K232R (kinase dead)

    Promoter

    CMV

    Available Since

    Aug. 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTOX3

    Plasmid

    #127448

    Purpose

    Encodes Tse2 toxin. CAM-R

    Depositor

    Matthew Waldor

    Article

    Lazarus et al Appl Environ Microbiol. 2019 Jun 14. pii

    Insert

    tse2 toxin
    

    Expression

    Bacterial

    Promoter

    pRha

    Available Since

    July 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. Str-KDEL_ST-SBP-mCherry

    Plasmid

    #65265

    Purpose

    synchronize trafficking of ST from the ER (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    Str-KDEL and truncated sialyltransferase (ST6GAL1 Human)
    

    Tags

    Streptavidin Binding Protein (SBP) and mCherry

    Expression

    Mammalian

    Mutation

    contains only amino acids 1 to 43 of ST

    Promoter

    CMV

    Available Since

    June 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pWZL-Neo-Myr-Flag-DEST

    Plasmid

    #15300

    Purpose

    a Gateway-compatible retroviral destination vector which adds a myristoylation sequence and a FLAG tag to each introduced ORF

    Depositor

    Jean Zhao

    Article

    Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    Flag and Myr

    Expression

    Mammalian

    Available Since

    July 12, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3-EGFP-Cdc42(Q61L)

    Plasmid

    #12600

    Depositor

    Klaus Hahn

    Article

    Nalbant et al Science. 2004 Sep 10. 305(5690):1615-9.

    Insert

    EGFP-Cdc42 (Q61L) (CDC42 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Q61L

    Available Since

    Oct. 16, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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19. Dyn2-pmCherryN1

    Plasmid

    #27689

    Depositor

    Christien Merrifield

    Article

    Taylor et al PLoS Biol. 2011 Mar . 9(3):e1000604.

    Insert

    Dynamin 2 (DNM2 Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Available Since

    May 4, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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20. 3336 pcDNA3 Bcl-2

    Plasmid

    #8768

    Depositor

    Stanley Korsmeyer

    Article

    Yamamoto et al Mol Cell Biol 1999 Dec;19(12):8469-78.

    Insert

    Bcl-2 (BCL2 Human)
    

    Expression

    Mammalian

    Available Since

    Nov. 14, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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