19,824 results

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pLL3.7 EGFPC2 TAZ

Plasmid

#66850

Purpose

Express GFP-fused TAZ by lentivirus

Depositor

Yutaka Hata

Article

Bao et al J Biochem. 2011 Aug;150(2):199-208. doi:

Insert

WWTR1/TAZ (WWTR1 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 17, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV/D374Y-hPCSK9

Plasmid

#58379

Purpose

Expresses GoF mutant human PCSK9 to be used for rAAV8-mediated gene transfer, hypercholesterolemia and atherosclerosis

Depositor

Jacob Bentzon

Article

Bjorklund et al Circ Res. 2014 May 23;114(11):1684-9. do

Insert

proprotein convertase subtilisin/kexin type 9 (PCSK9 Human)

Use

AAV

Expression

Mammalian

Mutation

D374Y

Promoter

HCRApoE/hAAT

Available Since

Aug. 26, 2014

Availability

Academic Institutions and Nonprofits only

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p201N Cas9

Plasmid

#59175

Purpose

Cas9 driven by double 35S, nptII for plant selection, I-PpoI site to accept gRNA from pUC gRNA Shuttle

Depositor

Wayne Parrott

Article

Jacobs et al BMC Biotechnol. 2015 Mar 12;15:16. doi:

Inserts

Cas9
nptII

Use

CRISPR

Promoter

2x35S and StUbi-3P

Available Since

Sept. 26, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3-PBZ-mRuby2

Plasmid

#110650

Purpose

expresses a PAR-binding (PBZ) domain fused to mRuby2

Depositor

Chris Lord

Article

Krastev et al Nat Commun. 2018 May 22;9(1):2016. doi:

Insert

PBZ domain from APLF, amino acids 371-451 (APLF Human)

Tags

mRuby2

Expression

Mammalian

Available Since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

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eMagB-iRFP-Mito

Plasmid

#162250

Purpose

Blue-light dependent heterodimerization system

Depositor

Pietro De Camilli

Article

Benedetti et al Elife. 2020 Nov 11;9. pii: 63230. doi: 1

Insert

eMagB-iRFP-OMP25

Expression

Mammalian

Promoter

beta-actin promoter

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pAGM51323

Plasmid

#153224

Purpose

Level 1 position 2 containing the Arabidopsis Rps5a promoter, Cas9 (Zcasi), and the nos terminator

Depositor

Sylvestre Marillonnet

Article

Grutzner et al Plant Commun. 2020 Nov 23;2(2):100135. d

Insert

Arabidopsis Rps5a promoter, Cas9 (Zcasi), nos terminator

Use

Synthetic Biology

Available Since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pcdna3.1 hciap1

Plasmid

#8311

Depositor

Jon Ashwell

Article

Li et al Nature 2002 Mar 21;416(6878):345-7.

Insert

cIAP1 (BIRC2 Human)

Tags

myc

Expression

Mammalian

Available Since

April 4, 2006

Availability

Academic Institutions and Nonprofits only

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EMTB-mScarletI

Plasmid

#137801

Purpose

Visualization of microtubules

Depositor

Dorus Gadella

Article

Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

Insert

Ensconsin microtubule binding domain

Expression

Mammalian

Promoter

CMV

Available Since

March 30, 2020

Availability

Academic Institutions and Nonprofits only

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pCW57.1-Tet-UPF1WT (pRKB264)

Plasmid

#99146

Purpose

Inducible lentiviral construct to express FLAG-tagged UPF1wt

Depositor

Robert Bradley

Article

Feng et al Mol Cell. 2017 Jul 20;67(2):239-251.e6.

Insert

UPF1 (UPF1 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

First 127 codons are optimized to reduce GC conte…

Promoter

pTight

Available Since

Nov. 27, 2017

Availability

Academic Institutions and Nonprofits only

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RFP-ATF4

Plasmid

#125237

Purpose

ATF4 expression vector

Depositor

Rozita Yarmand

Article

Bagheri-Yarmand et al J Biol Chem. 2015 May 1;290(18):11749-61

Insert

ATF4 (ATF4 Human)

Tags

RFP

Expression

Mammalian

Available Since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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pLJC6-2XFLAG-TMEM192

Plasmid

#104435

Purpose

Expresses 2XFLAG-TMEM192 in mammalian cells

Depositor

David Sabatini

Article

Wyant et al Cell. 2017 Oct 19;171(3):642-654.e12. do

Insert

TMEM-192 (TMEM192 Human)

Use

Lentiviral

Tags

2XFLAG

Expression

Mammalian

Promoter

Ubiquitin

Available Since

July 2, 2018

Availability

Academic Institutions and Nonprofits only

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pDONR P2R-P3-SV40 polyA signal

Plasmid

#48627

Purpose

Contributes a cassette containing an SV40 polyA signal as the 3’-module during MultiSite Gateway cloning of chimeric cDNAs, when a peptide module at the C-terminal end is not required.

Depositor

Anna Planas, Tomas Santalucia

Article

Buj et al BMC Mol Biol. 2013 Aug 20;14(1):18. doi:

Insert

SV40 early polyadenylation signal cassette

Use

Gateway entry vector

Promoter

none

Available Since

April 9, 2014

Availability

Academic Institutions and Nonprofits only

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vGAT-pH pcaggs SE

Plasmid

#78578

Purpose

image recycling of VGAT in live neurons

Depositor

Susan Voglmaier

Article

Santos et al J Neurosci. 2013 Jun 26;33(26):10634-46.

Insert

vGAT-pHluorin C-term (Slc32a1 Rat)

Tags

superecliptic pHluorin

Expression

Mammalian

Promoter

chicken actin

Available Since

July 13, 2016

Availability

Academic Institutions and Nonprofits only
F-MED26

Plasmid

#15367

Depositor

Joan Conaway, Ronald Conaway

Article

Sato et al Mol Cell. 2004 Jun 4. 14(5):685-91.

Insert

MED26 (MED26 Human)

Tags

FLAG

Expression

Mammalian

Mutation

N/A

Available Since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only
pU6b:sgRNA#3

Plasmid

#64247

Purpose

expresses sgRNA under U6b promoter

Depositor

Wenbiao Chen

Article

Yin et al Genetics. 2015 Apr 8. pii: genetics.115.

Type

Empty backbone

Use

CRISPR

Available Since

May 4, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Lyn-AKAR4

Plasmid

#61620

Purpose

Lipid raft-targeted FRET biosensor for monitoring PKA activity

Depositor

Jin Zhang

Article

Depry et al Mol Biosyst. 2011 Jan;7(1):52-8. doi: 10

Insert

Lyn-AKAR4

Tags

Cerulean, N-terminal targeting sequence from Lyn …

Expression

Mammalian

Promoter

CMV

Available Since

March 13, 2015

Availability

Academic Institutions and Nonprofits only

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FAS promoter luciferase

Plasmid

#8890

Depositor

Bruce Spiegelman

Article

Kim et al J Clin Invest. 1998 Jan 1. 101(1):1-9.

Insert

Fatty Acid Synthetase Promoter (Fasn unknown - rat or mouse, Rat)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Available Since

Nov. 14, 2005

Availability

Academic Institutions and Nonprofits only

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pMRXIP VPS16-GFP

Plasmid

#67023

Purpose

Human VPS16 ORF was inserted into pMRXIP GFP-N vector

Depositor

Noboru Mizushima

Article

Jiang et al Mol Biol Cell. 2014 Apr;25(8):1327-37. d

Insert

Homo sapiens vacuolar protein sorting 16 homolog (S. cerevisiae) (VPS16) (VPS16 Human)

Use

Retroviral

Tags

GFP

Promoter

CMV

Available Since

Aug. 18, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA5FRT/Flag-KIAA1267

Plasmid

#29758

Depositor

Joan Conaway

Article

Cai et al J Biol Chem. 2010 Feb 12. 285(7):4268-72

Insert

KIAA1267 (KANSL1 Human)

Tags

Flag

Expression

Mammalian

Available Since

Jan. 30, 2014

Availability

Academic Institutions and Nonprofits only

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pLenti4/V5-DEST-P85

Plasmid

#40219

Depositor

Lynda Chin

Insert

P85 alpha (PIK3R1 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 11, 2012

Availability

Academic Institutions and Nonprofits only