We narrowed to 67,891 results for: TOR

1. eIF3k- pMK289 (mAID-mClover-NeoR) plasmid

   Plasmid

   #192243

   Purpose

   knockin donor vector of mAID-mClover-NeoR to C-terminus of endogenous human eIF3k

   Depositor

   Dieter Wolf

   Article

   Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

   Insert

   eIF3k-knockin-homology arm-mAID-mClover-NeoR (EIF3K Human)
   

   Expression

   Mammalian

   Available Since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. eIF3k- pMK293 (mAID-mCherry2-Hygro) plasmid

   Plasmid

   #192244

   Purpose

   knockin donor vector of mAID-mCherry2-Hygro to C-terminus of endogenous human eIF3k

   Depositor

   Dieter Wolf

   Article

   Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

   Insert

   eIF3k-knockin-homology arm-mAID-mCherry2-Hygro (EIF3K Human)
   

   Expression

   Mammalian

   Available Since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. eIF3b- pMK289 (mAID-mClover-NeoR) plasmid

   Plasmid

   #192237

   Purpose

   knockin donor vector of mAID-mClover-NeoR to C-terminus of endogenous human eIF3b

   Depositor

   Dieter Wolf

   Article

   Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

   Insert

   eIF3b-knockin-homology arm-mAID-mClover-NeoR (EIF3B Human)
   

   Expression

   Mammalian

   Available Since

   Jan. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. eIF3b- pMK293 (mAID-mCherry2-Hygro) plasmid

   Plasmid

   #192238

   Purpose

   knockin donor vector of mAID-mCherry2-Hygro to C-terminus of endogenous human eIF3b

   Depositor

   Dieter Wolf

   Article

   Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

   Insert

   eIF3b-knockin-homology arm-mAID-mCherry2-Hygro (EIF3B Human)
   

   Expression

   Mammalian

   Available Since

   Jan. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSLED.CaRPv1

   Plasmid

   #193018

   Purpose

   Excitatory/Inhibitory neuron calcium indicator (hSyn promoter, Synrg exon, jRGECO1a/jGCaMP7b)

   Depositor

   Jonathan Ling

   Article

   Ling et al Nat Commun. 2022 Oct 1;13(1):5773. doi:

   Insert

   Bichromatic calcium indicator (jGCaMP7b and jRGECO1a)
   

   Use

   AAV

   Available Since

   Dec. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDR111_mannose_Klf4Gata-3

   Plasmid

   #188401

   Purpose

   Expresses mouse transcription factors Klf4 and Gata-3 in an operon from D-mannose promoter for Bacillus subtilis. The secretion peptide PhoD is fused to both factors.

   Depositor

   Christopher Contag

   Article

   Madsen et al Commun Biol. 2022 Aug 30;5(1):888. doi:

   Inserts

   Klf4 (Klf4 Mouse)
   Gata-3 (Gata3 Mouse)
   

   Tags

   PhoD secretion peptide

   Expression

   Bacterial

   Mutation

   See Depositor Comments Below

   Promoter

   D-mannose

   Available Since

   Aug. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. dCas9-p300 PHD-HAT

   Plasmid

   #179545

   Purpose

   encodes S. pyogenes dCas9 with c-terminal fusion of human p300 PHD-HAT domain (aa 1243-1664) driven by EF-1alpha promoter

   Depositor

   Isaac Hilton

   Article

   Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78

   Insert

   S. pyogenes dCas9 with c-terminal fusion of human p300 PHD-HAT domain (aa 1243-1664) (EP300 Human)
   

   Use

   Lentiviral

   Tags

   Flag Tag

   Expression

   Mammalian

   Mutation

   D10A; H840A in S.pyogenes Cas9

   Promoter

   EF-1alpha

   Available Since

   Feb. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. dCas9-CBP RING-PHD-HAT

   Plasmid

   #179549

   Purpose

   encodes S. pyogenes dCas9 with c-terminal fusion of human CBP RING-PHD-HAT domain (aa 1191-1701) driven by EF-1alpha promoter

   Depositor

   Isaac Hilton

   Article

   Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78

   Insert

   S. pyogenes dCas9 with c-terminal fusion of human CBP RING-PHD-HAT domain (aa 1191-1701) (CREBBP Human)
   

   Use

   Lentiviral

   Tags

   Flag Tag

   Expression

   Mammalian

   Mutation

   D10A; H840A in S.pyogenes Cas9

   Promoter

   EF-1alpha

   Available Since

   Feb. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. dCas9-CBP PHD-HAT

   Plasmid

   #179548

   Purpose

   encodes S. pyogenes dCas9 with c-terminal fusion of human CBP PHD-HAT domain (aa 1279-1701) driven by EF-1alpha promoter

   Depositor

   Isaac Hilton

   Article

   Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78

   Insert

   S. pyogenes dCas9 with c-terminal fusion of human CBP PHD-HAT domain (aa 1279-1701) (CREBBP Human)
   

   Use

   Lentiviral

   Tags

   Flag Tag

   Expression

   Mammalian

   Mutation

   D10A; H840A in S.pyogenes Cas9

   Promoter

   EF-1alpha

   Available Since

   Feb. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. dCas9-p300 RING-PHD-HAT

    Plasmid

    #179546

    Purpose

    encodes S. pyogenes dCas9 with c-terminal fusion of human p300 RING-PHD-HAT domain (aa 1155-1664) driven by EF-1alpha promoter

    Depositor

    Isaac Hilton

    Article

    Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78

    Insert

    S. pyogenes dCas9 with c-terminal fusion of human p300 RING-PHD-HAT domain (aa 1155-1664) (EP300 Human)
    

    Use

    Lentiviral

    Tags

    Flag Tag

    Expression

    Mammalian

    Mutation

    D10A; H840A in S.pyogenes Cas9

    Promoter

    EF-1alpha

    Available Since

    Feb. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBIG1b_nsp10-6His-3xFlag (SARS-CoV-2)

    Plasmid

    #169162

    Purpose

    Baculoviral transfer vector to express SARS-CoV-2 nsp10 in insect cells

    Depositor

    John Diffley

    Article

    Canal et al Biochem J. 2021 Jul 16;478(13):2445-2464

    Insert

    nsp10-6His-3xFlag (ORF1ab Synthetic, SARS-CoV-2)
    

    Tags

    6His-3xFlag

    Expression

    Insect

    Mutation

    Codon optimised for insect cells (Spodoptera frug…

    Promoter

    Polyhedrin

    Available Since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3-sPDGFRalpha-Fc-Y206S

    Plasmid

    #136439

    Purpose

    Mammalian expression plasmid for soluble ectodomain of PDGFR-alpha fused to IgG1 Fc (Y206S mutant)

    Depositor

    Erik Procko

    Article

    Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.

    Insert

    PDGFRalpha (PDGFRA Human)
    

    Tags

    Fc region of IgG1 (a.a. Asp-221 to Lys-447) and L…

    Expression

    Mammalian

    Mutation

    Extracellular domain of PDGFR-alpha (a.a. 1-524) …

    Promoter

    CMV

    Available Since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3-sPDGFRalpha-Fc-V242K

    Plasmid

    #136440

    Purpose

    Mammalian expression plasmid for soluble ectodomain of PDGFR-alpha fused to IgG1 Fc (V242K mutant)

    Depositor

    Erik Procko

    Article

    Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.

    Insert

    PDGFRalpha (PDGFRA Human)
    

    Tags

    Fc region of IgG1 (a.a. Asp-221 to Lys-447) and L…

    Expression

    Mammalian

    Mutation

    Extracellular domain of PDGFR-alpha (a.a. 1-524) …

    Promoter

    CMV

    Available Since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEN_TT 3XFLAG-S37A-GATA6-3XAU1_RNAi resistant

    Plasmid

    #72924

    Purpose

    Gateway entry vector for an inducible N-terminally 3XFLAG-tagged and C-terminally AU1-tagged, RNAi resistant human S37A-GATA6

    Depositor

    Kevin Janes

    Article

    Chitforoushzadeh et al Sci Signal. 2016 Jun 7;9(431):ra59. doi:

    Insert

    GATA Binding Protein 6 (GATA6 Human)
    

    Use

    Entry vector for gateway cloning

    Tags

    3XAU1 and 3XFLAG

    Expression

    Bacterial

    Mutation

    Serine#37 changed to Alanine;Silent mutations mad…

    Promoter

    TRE tight

    Available Since

    July 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV-Sport6-CD63-pHluorin

    Plasmid

    #130901

    Purpose

    Expression of CD63-pHluorin for visualization of multivesicular body-plasma membrane fusion.

    Depositor

    DM Pegtel

    Article

    Verweij et al J Cell Biol. 2018 Mar 5;217(3):1129-1142

    Insert

    CD63-pHluorin (CD63 Aequorea victoria, Human)
    

    Tags

    pHluorin

    Expression

    Mammalian

    Mutation

    pHluorin inserted between Gln-36 and Leu-37 in ex…

    Promoter

    CMV

    Available Since

    Oct. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3-ICUE4

    Plasmid

    #181846

    Purpose

    Fourth-generation FRET-based Indicator of cAMP Using Epac. Genetically encoded cAMP indicator. Contains high-sensitivity Epac Q270E mutation.

    Depositor

    Jin Zhang

    Article

    Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

    Insert

    ICUE4 (RAPGEF3 Human)
    

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Mutation

    Glutamine 122 in Epac1 domain mutated to glutamat…

    Promoter

    CMV

    Available Since

    June 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV-Sport6-CD9-pHluorin

    Plasmid

    #130905

    Purpose

    Expression of CD9-pHluorin for visualization of multivesicular body-plasma membrane fusion.

    Depositor

    DM Pegtel

    Article

    Verweij et al J Cell Biol. 2018 Mar 5;217(3):1129-1142

    Insert

    CD9-pHluorin (CD9 Aequorea victoria, Human)
    

    Tags

    pHluorin

    Expression

    Mammalian

    Mutation

    phluorin inserted between Asn-50 and Asn-51 in ex…

    Promoter

    CMV

    Available Since

    Feb. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV-Sport6-CD81-pHluorin

    Plasmid

    #130903

    Purpose

    Expression of CD81-pHluorin for visualization of multivesicular body-plasma membrane fusion.

    Depositor

    DM Pegtel

    Article

    Verweij et al J Cell Biol. 2018 Mar 5;217(3):1129-1142

    Insert

    CD81-pHluorin (CD81 aequorea victoria, Human)
    

    Tags

    pHluorin

    Expression

    Mammalian

    Mutation

    pHluorin inserted between Leu-49 and Gly-50 in ex…

    Promoter

    CMV

    Available Since

    Oct. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. GST-GGA3-VHS-GAT

    Plasmid

    #173719

    Purpose

    VHS-GAT domain in GGA3 can bind to an active form of ARF1 protein. Using this construct, you can assess the activity of ARF1 protein.

    Depositor

    Juan Bonifacino

    Article

    Dell'Angelica et al J Cell Biol. 2000 Apr 3;149(1):81-94. do

    Insert

    GGA3 (GGA3, GGA2, GGA1 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Available Since

    Sept. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV_CaMKIIa(0.4)-PdCO-EGFP-WPRE

    Plasmid

    #198514

    Purpose

    Expresses optimized PdCO in frame with EGFP under control of minimal CamKIIa promotor.

    Depositor

    Ofer Yizhar

    Article

    Wietek et al Nat Methods. 2024 May 29. doi: 10.1038/s

    Insert

    PdCO
    

    Use

    AAV

    Tags

    EGFP and Rho1D4

    Expression

    Mammalian

    Promoter

    CaMKIIα minimal promotor (0.4kb)

    Available Since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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21. pEP4 E02S EN2K

    Plasmid

    #20925

    Purpose

    used in the derivation of human iPS cells using non-integrating episomal vectors; expresses Oct4 and Sox2; Nanog and Klf4

    Depositor

    James Thomson

    Article

    Yu et al Science. 2009 Mar 26.

    Insert

    Oct4 and Sox2; Nanog and Klf4 (SOX2, POU5F1, NANOG, KLF4 Human)
    

    Use

    Episomal/ebna

    Available Since

    May 20, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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22. pAAV-U6-sgRNA(CTRL)-CMV-eGFP

    Plasmid

    #194017

    Purpose

    Expresses a gRNA that targets the LacZ gene (serves as control) and eGFP

    Depositor

    Larry Young

    Article

    Boender et al Sci Adv. 2023 Jun 2;9(22):eadf4950. doi:

    Inserts

    sgRNA(CTRL)
    eGFP
    

    Use

    AAV and CRISPR

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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23. Tet-O-FUW-EGR1-P2A-mCherry

    Plasmid

    #203856

    Purpose

    Tet-inducible lentiviral plasmid expressing EGR1-P2A-mCherry, with ORF specific barcode close to 3'LTR site

    Depositor

    Andrea Califano

    Article

    Obradovic et al Cancer Cell. 2023 May 8;41(5):933-949.e1

    Insert

    EGR1 (EGR1 Human)
    

    Expression

    Mammalian

    Available Since

    Sept. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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24. G protein alpha-s-CFP

    Plasmid

    #55793

    Purpose

    G protein alpha-s internally tagged with ECFP and EE epitope

    Depositor

    Catherine Berlot

    Article

    Hynes et al J Biol Chem. 2004 Oct 15;279(42):44101-1

    Insert

    G-alpha-s-EE-ECFP (Gnas Rat)
    

    Tags

    ECFP flanked by SGGGS on each side was inserted i…

    Expression

    Mammalian

    Mutation

    His was substituted for Asn164 in ECFP (Clontech)…

    Promoter

    CMV

    Available Since

    Aug. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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25. G protein alpha-s-GFP

    Plasmid

    #66083

    Purpose

    G protein alpha-s internally tagged with EGFP and EE epitope

    Depositor

    Catherine Berlot

    Article

    Hynes et al J Biol Chem. 2004 Oct 15;279(42):44101-1

    Insert

    G-alpha-s-EE-EGFP (Gnas Rat)
    

    Tags

    EGFP flanked by SGGGS on each side was inserted i…

    Expression

    Mammalian

    Mutation

    BamHI sites in the alpha-s cDNA were removed and …

    Promoter

    CMV

    Available Since

    Aug. 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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26. pME-mCherry-PA-Rac1

    Plasmid

    #169042

    Purpose

    Middle entry vector for Gateway cloning that contains an mCherry fused with an optimized photoactivatable Rac1 gene insert

    Depositor

    Paola Arlotta

    Article

    Harris et al Dev Cell. 2020 Jun 8;53(5):577-588.e7. d

    Insert

    PA-Rac-1 (RAC1 Avena sativa (oat), Human)
    

    Use

    Gateway entry cloning vector

    Tags

    6X His and Fused with mCherry

    Mutation

    Rac1 starts at I4 and contains mutations Q61L, E9…

    Available Since

    Jan. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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27. pEF2-FL-hMer_gR1

    Plasmid

    #225993

    Purpose

    Expresses a chimeric contruct having N-terminal human MerTK and C-terminal human Interferon Gamma Receptor

    Depositor

    Sergei Kotenko, Raymond Birge

    Article

    Tsou et al J Biol Chem. 2014 Sep 12;289(37):25750-6

    Insert

    human MerTK N-Terminal human Interferon Gamma R1 Receptor C-Terminal fusion (MERTK, IFNGR1 Human)
    

    Expression

    Mammalian

    Promoter

    EF1-a

    Available Since

    Nov. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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28. TRIPZ-CTID195-GSQ-SUMO1nc-PURO

    Plasmid

    #208035

    Purpose

    Enables inducible expression of C-terminal Split-TurboID-fused SUMO1nc, to perform SUMO-ID; nc = non-cleavable mutation; selection with puromycin

    Depositor

    James Sutherland, Rosa Barrio

    Article

    Barroso-Gomila et al Nat Commun. 2021 Nov 18;12(1):6671. doi:

    Insert

    SUMO1nc (SUMO1 Human)
    

    Use

    Lentiviral

    Tags

    Myc, C-terminal Split-TurboID

    Mutation

    Q94P Mutation near C-terminus suppresses cleavage…

    Promoter

    tetO/UBC

    Available Since

    Nov. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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29. pAAV-CaMKIIa(0.4)-PdCO-mScarlet-WPRE

    Plasmid

    #198508

    Purpose

    Expresses optimized PdCO in frame with mScarlet under control of minimal CamKIIa promotor.

    Depositor

    Ofer Yizhar

    Article

    Wietek et al Nat Methods. 2024 May 29. doi: 10.1038/s

    Insert

    PdCO
    

    Use

    AAV

    Tags

    Rho1D4 and mScarlet

    Expression

    Mammalian

    Promoter

    CaMKIIα minimal promotor (0.4kb)

    Available Since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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30. PGL4.11-COL1A1mSMAD

    Plasmid

    #230917

    Purpose

    Tested the COL1A1 promoter activity after abolition of the proximal SMAD binding sites in the promoter.

    Depositor

    Avi Rosenberg

    Article

    Yang et al Lab Invest. 2024 May;104(5):100336. doi:

    Insert

    Human COL1A1 promoter with mutant SMAD binding sequence (COL1A1 Human)
    

    Use

    Luciferase

    Tags

    Luciferase-luc2p

    Expression

    Bacterial and Mammalian

    Mutation

    COL1A1 promoter with mutant potential SMAD bindin…

    Promoter

    COL1A1

    Available Since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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