We narrowed to 9,638 results for: Ada;

1. pTRIP TRE BI_SEPT9_i3-14-GFP10_GFP11-14-SEPT7 Gmut2

   Plasmid

   #180361

   Purpose

   mammalian co-expression of human SEPT9_i3 fused to GFP10 and of human SEPT7 Gmut2 fused to GFP11

   Depositor

   Manos Mavrakis

   Article

   Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

   Inserts

   SEPTIN7 (SEPTIN7 Human)
   SEPTIN9 (SEPTIN9 Human)
   IRES-TagBFP
   

   Use

   Lentiviral

   Tags

   GFP10 and GFP11

   Expression

   Mammalian

   Mutation

   SEPT7 H279D

   Promoter

   bidirectional TRE

   Available since

   Dec. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pTRIP TRE BI_GFP11-14-SEPT7_GFP10-14-SEPT7

   Plasmid

   #180347

   Purpose

   mammalian co-expression of human SEPT7 fused to GFP10 and of human SEPT7 fused to GFP11

   Depositor

   Manos Mavrakis

   Article

   Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

   Inserts

   SEPTIN7 (SEPTIN7 Human)
   SEPTIN7 (SEPTIN7 Human)
   IRES-TagBFP
   

   Use

   Lentiviral

   Tags

   GFP10 and GFP11

   Expression

   Mammalian

   Mutation

   Promoter

   bidirectional TRE

   Available since

   Dec. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pnEA-vH_His-TEV-SEPT2 NCmut-msfGFP_SEPT6

   Plasmid

   #180312

   Purpose

   bacterial co-expression of human SEPT2 NCmut fused to monomeric superfolder GFP and of human SEPT6

   Depositor

   Manos Mavrakis

   Article

   Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

   Inserts

   SEPTIN2 (SEPTIN2 Human)
   SEPTIN6 (SEPTIN6 Human)
   

   Use

   Tags

   His6-TEV and msfGFP

   Expression

   Bacterial

   Mutation

   SEPT2 F20D, V27D

   Promoter

   Available since

   Dec. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. IL2-AH-2A-Cherry-RhoAQ63L in pmCherryN1

   Plasmid

   #187287

   Purpose

   Express pmCherry-IL2-AH-2A-RhoA Q63L

   Depositor

   Alpha Yap

   Article

   Budnar et al Dev Cell. 2019 Jun 17;49(6):894-906.e12.

   Insert

   interleukin-2 receptor alpha-subunit, anillin, RhoA (IL2, ANLN, RHOA Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   AH-2A-RhoA Q63L

   Promoter

   CMV

   Available since

   Sept. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. IL2-AHDC2-2A-CherryRhoAQ63L in pmCherryN1

   Plasmid

   #187292

   Purpose

   Express pmCherry-IL2-AH-deltaC2-2A-RhoA Q63L

   Depositor

   Alpha Yap

   Article

   Budnar et al Dev Cell. 2019 Jun 17;49(6):894-906.e12.

   Insert

   interleukin-2 receptor alpha-subunit, anillin, RhoA (IL2, ANLN, RHOA Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   AH-deltaC2, RhoA Q63L

   Promoter

   CMV

   Available since

   Sept. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pVRC8400-SARS-CoV-2-RBD-SD1-BA.2-AVI

   Plasmid

   #184534

   Purpose

   Expresses SARS-CoV-2 RBD-SD1 domain from the BA.2 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag

   Depositor

   Peter Kwong

   Article

   Teng et al PLoS One. 2022 May 24;17(5):e0268767. do

   Insert

   SARS-CoV-2-RBD-SD1-BA.2 (S )
   

   Use

   Tags

   AVI, HRV3C, and scFc

   Expression

   Mammalian

   Mutation

   G339D, S371F, S373P, S375F, T376A, D405N, R408S, …

   Promoter

   Available since

   June 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCMV-T7-NLS(SV40)-anCas(SCA)-BPNLS(SV40)-P2A-EGFP (LTH1378)

   Plasmid

   #185484

   Purpose

   CMV and T7 promoter expression plasmid for human codon usage anCas(SCA) with an N-terminal NLS, C-terminal bi-partite NLS, and P2A-eGFP

   Depositor

   Raul Perez-Jimenez, Benjamin Kleinstiver

   Article

   Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:

   Insert

   human codon usage NLS-anCas(SCA)-BPNLS-P2A-eGFP
   

   Use

   In vitro transcription; t7 promoter

   Tags

   BPNLS-P2A-eGFP and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and T7

   Available since

   June 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCMV-T7-NLS(SV40)-anCas(PCA)-BPNLS(SV40)-P2A-EGFP (LTH1441)

   Plasmid

   #185486

   Purpose

   CMV and T7 promoter expression plasmid for human codon usage anCas(PCA) with an N-terminal NLS, C-terminal bi-partite NLS, and P2A-eGFP

   Depositor

   Raul Perez-Jimenez, Benjamin Kleinstiver

   Article

   Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:

   Insert

   human codon usage NLS-anCas(PCA)-BPNLS-P2A-eGFP
   

   Use

   In vitro transcription; t7 promoter

   Tags

   BPNLS-P2A-eGFP and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and T7

   Available since

   June 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCMV-T7-NLS(SV40)-anCas(FCA)-BPNLS(SV40)-P2A-EGFP (LTH1380)

   Plasmid

   #185487

   Purpose

   CMV and T7 promoter expression plasmid for human codon usage anCas(FCA) with an N-terminal NLS, C-terminal bi-partite NLS, and P2A-eGFP

   Depositor

   Raul Perez-Jimenez, Benjamin Kleinstiver

   Article

   Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:

   Insert

   human codon usage NLS-anCas(FCA)-BPNLS-P2A-eGFP
   

   Use

   In vitro transcription; t7 promoter

   Tags

   BPNLS-P2A-eGFP and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and T7

   Available since

   June 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCMV-T7-nSpRY(H840A)-BPNLS(SV40)-3xFLAG-P2A-EGFP (HES395)

    Plasmid

    #185489

    Purpose

    CMV and T7 promoter expression plasmid for human codon usage nSpRY(H840A) with a C-terminal bi-partite NLS, 3x FLAG, and P2A-eGFP

    Depositor

    Raul Perez-Jimenez, Benjamin Kleinstiver

    Article

    Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:

    Insert

    human codon usage nSpRY(H840A)-BPNLS-P2A-eGFP
    

    Use

    In vitro transcription; t7 promoter

    Tags

    BPNLS-3xFLAG-P2A-eGFP

    Expression

    Mammalian

    Mutation

    H840A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N13…

    Promoter

    CMV and T7

    Available since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCMV-T7-nSpCas9(D10A)-BPNLS(SV40)-3xFLAG-P2A-EGFP (HES24)

    Plasmid

    #185492

    Purpose

    CMV and T7 promoter expression plasmid for human codon usage nSpCas9(D10A) with a C-terminal bi-partite NLS, 3x FLAG, and P2A-eGFP

    Depositor

    Raul Perez-Jimenez, Benjamin Kleinstiver

    Article

    Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:

    Insert

    human codon usage nSpCas9(D10A)-BPNLS-P2A-eGFP
    

    Use

    In vitro transcription; t7 promoter

    Tags

    BPNLS-3xFLAG-P2A-eGFP

    Expression

    Mammalian

    Mutation

    D10A

    Promoter

    CMV and T7

    Available since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pVRC8400-SARS-CoV-2-S2P-BA.2-AVI

    Plasmid

    #184531

    Purpose

    Expresses SARS-CoV-2 S2P protein from the BA.2 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag

    Depositor

    Peter Kwong

    Article

    Teng et al PLoS One. 2022 May 24;17(5):e0268767. do

    Insert

    SARS-CoV-2-S2P-BA.2 (S )
    

    Use

    Tags

    AVI, HRV3C, and scFc

    Expression

    Mammalian

    Mutation

    T19I, L24-, P25-, P26-, A27S, G142D, V213G, G339D…

    Promoter

    Available since

    May 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pVRC8400-SARS-CoV-2-RBD-SD1-K417N-L452R-T478K-AVI

    Plasmid

    #176457

    Purpose

    Expresses SARS-CoV-2 RBD-SD1 domain containing K417N-L452R-T478K mutations with single chain Fc, HRV3C protease cleavage site, and AVI tag

    Depositor

    Peter Kwong

    Article

    Teng et al PLoS One. 2022 May 24;17(5):e0268767. do

    Insert

    SARS-CoV-2-RBD-SD1-K417N-L452R-T478K (S )
    

    Use

    Tags

    AVI, HRV3C, and scFc

    Expression

    Mammalian

    Mutation

    K417N, L452R, T478K

    Promoter

    Available since

    March 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pVRC8400-SARS-CoV-2-RBD-SD1-K417N-E484K-N501Y-AVI

    Plasmid

    #176450

    Purpose

    Expresses SARS-CoV-2 RBD-SD1 domain containing K417N-E484K-N501Y mutations with single chain Fc, HRV3C protease cleavage site, and AVI tag

    Depositor

    Peter Kwong

    Article

    Teng et al PLoS One. 2022 May 24;17(5):e0268767. do

    Insert

    SARS-CoV-2-RBD-SD1-K417N-E484K-N501Y (S )
    

    Use

    Tags

    AVI, HRV3C, and scFc

    Expression

    Mammalian

    Mutation

    K417N, E484K, N501Y

    Promoter

    Available since

    March 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pVRC8400-SARS-CoV-2-RBD-SD1-K417T-E484K-N501Y-AVI

    Plasmid

    #176451

    Purpose

    Expresses SARS-CoV-2 RBD-SD1 domain containing K417T-E484K-N501Y mutations with single chain Fc, HRV3C protease cleavage site, and AVI tag

    Depositor

    Peter Kwong

    Article

    Teng et al PLoS One. 2022 May 24;17(5):e0268767. do

    Insert

    SARS-CoV-2-RBD-SD1-K417T-E484K-N501Y (S )
    

    Use

    Tags

    AVI, HRV3C, and scFc

    Expression

    Mammalian

    Mutation

    K417T, E484K, N501Y

    Promoter

    Available since

    March 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pVRC8400-SARS-CoV-2-S2P-B.1.617-E154K-L452R-E484Q-D614G-P681R-AVI

    Plasmid

    #176331

    Purpose

    Expresses SARS-CoV-2 S2P protein from the B.1.617 variant containing E154K-L452R-E484Q-D614G-P681R mutations with single chain Fc, HRV3C protease cleavage site, and AVI tag

    Depositor

    Peter Kwong

    Article

    Teng et al PLoS One. 2022 May 24;17(5):e0268767. do

    Insert

    SARS-CoV-2-S2P-B.1.617 (S )
    

    Use

    Tags

    AVI, HRV3C, and scFc

    Expression

    Mammalian

    Mutation

    E154K, L452R, E484Q, D614G, P681R

    Promoter

    Available since

    March 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pHD-dsRed-attP_dCrk_flankingHRs

    Plasmid

    #131141

    Purpose

    Contains ~1kb homology regions flanking Drosophila crk locus. Used to make crk null mutant. Homology regions cloned into AarI and SapI sites of pHD-dsRed-attP (Harrison, O'Connor-Giles, Wildonger).

    Depositor

    Mark Peifer

    Article

    Spracklen et al Mol Biol Cell. 2019 Jul 18:mbcE19050302.

    Inserts

    Crk Upstream Homology Region (Crk Fly)
    Crk Downstream Homology Region (Crk Fly)
    

    Use

    CRISPR and Cre/Lox; Homology directed repair temp…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pGL4.23 C3_27

    Plasmid

    #60307

    Purpose

    This plasmid contains a human pancreatic islet active enhancer, a minimal promoter and a luc2 gene.

    Depositor

    Jorge Ferrer

    Article

    Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

    Insert

    C16orf80 enhancer (CFAP20 Human)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

19. pGL4.23 C3_28

    Plasmid

    #60308

    Purpose

    This plasmid contains a human pancreatic islet active enhancer, a minimal promoter and a luc2 gene.

    Depositor

    Jorge Ferrer

    Article

    Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

    Insert

    CRY2 enhancer (CRY2 Human)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

20. pGL4.23 C3_32

    Plasmid

    #60311

    Purpose

    This plasmid contains a human pancreatic islet active enhancer, a minimal promoter and a luc2 gene.

    Depositor

    Jorge Ferrer

    Article

    Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

    Insert

    DGKB enhancer (DGKB Human)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 2, 2014

    Availability

    Academic Institutions and Nonprofits only