19,660 results

1. HA-cdc20

   Plasmid

   #11594

   Depositor

   Marc Kirschner

   Article

   Pfleger et al Genes Dev. 2001 Sep 15. 15(18):2396-407.

   Insert

   cdc20 (CDC20 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Available Since

   April 20, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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2. CMV-eGFP-Z1

   Plasmid

   #135049

   Purpose

   Enhanced Green fluorescent protein expression for mammalian cells under constitutive CMV Promoter in minimal backbone plasmid.

   Depositor

   Jordan Green

   Insert

   eGFP
   

   Use

   Synthetic Biology

   Expression

   Mammalian

   Promoter

   CMV Promoter

   Available Since

   May 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-hSyn-ChRmine-mScarlet-WPRE

   Plasmid

   #130994

   Purpose

   AAV vector to drive the expression of ChRmine-mScarlet under the control of human synapsin promoter

   Depositor

   Karl Deisseroth

   Article

   Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

   Insert

   ChRmine
   

   Use

   AAV

   Tags

   mScarlet

   Promoter

   hSyn

   Available Since

   Sept. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. SynTagMA_post

   Plasmid

   #119736

   Purpose

   Synaptic Tag for Mapping Activity

   Depositor

   Thomas Oertner

   Article

   Perez-Alvarez et al Nat Commun. 2020 May 18;11(1):2464. doi:

   Insert

   SynTagMA
   

   Use

   AAV

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   hSyn-1

   Available Since

   Feb. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3-TfR-OVA

   Plasmid

   #64600

   Purpose

   Expression of chicken ovalbumin fused to the transmembrane domain of transferrin receptor as a model antigen for study of MHC II presentation

   Depositor

   Sandra Diebold, Martin Zenke

   Article

   Diebold et al Gene Ther. 2001 Mar;8(6):487-93.

   Insert

   Ovalbumin
   

   Tags

   Transmembrane domain of Transferrin Receptor (TfR…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGOAL19

   Plasmid

   #20190

   Depositor

   Tanya Parish

   Article

   Parish et al Microbiology (Reading). 2000 Aug;146 ( P

   Type

   Empty backbone

   Use

   Marker cassette

   Available Since

   May 22, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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7. pNCS YFP

   Plasmid

   #91759

   Purpose

   Bacterial expression of a fluorescent protein, Yellow Fluorescent Protein (YFP)

   Depositor

   Erik Rodriguez, Roger Tsien

   Article

   Cubitt et al Trends Biochem Sci. 1995 Nov;20(11):448-

   Insert

   Venus YFP (A206K)
   

   Tags

   Hexa-Histidine tag, Xpress epitope for detection …

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   June 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA5/FRT/TO_IgKchL_HA_HaloTag9_myc_PDGFRtmb

   Plasmid

   #175544

   Purpose

   Expression of HaloTag9 at the outer leaflet of the plasma membrane of mammalian cells

   Depositor

   Kai Johnsson

   Article

   Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

   Insert

   IgKchL_HA-HaloTag9-myc_PDGFRtmb
   

   Expression

   Mammalian

   Mutation

   HaloTag9 = HaloTag7-Q165H-P174R

   Available Since

   Dec. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. AICSDP-47: CTNNB1-mEGFP

   Plasmid

   #109119

   Purpose

   Homology arms and mEGFP-linker sequence for N-terminus tagging of human CTNNB1

   Depositor

   Allen Institute for Cell Science

   Insert

   CTNNB1 Homology Arms with mEGFP-linker (CTNNB1 Human)
   

   Use

   CRISPR; Donor template

   Tags

   mEGFP-linker

   Expression

   Mammalian

   Mutation

   homology arms contain point mutations to disrupt …

   Available Since

   May 4, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. Flag-HA-USP22

    Plasmid

    #22575

    Depositor

    Wade Harper

    Article

    Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

    Insert

    USP22 (USP22 Human)
    

    Use

    Retroviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    None

    Available Since

    Nov. 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCW57.1-DUX4-CA

    Plasmid

    #99281

    Purpose

    All-in-one lentivector with tet-inducible DUX4 (codon sequences altered)

    Depositor

    Stephen Tapscott

    Article

    Jagannathan et al Hum Mol Genet. 2016 Oct 15;25(20):4419-4

    Insert

    DUX4 (codon altered) (DUX4 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    codon altered

    Promoter

    TRE

    Available Since

    Aug. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLenti-CAG-empty-ires-EGFP

    Plasmid

    #122953

    Purpose

    Lentiviral expression empty vector under CAG promoter with EGFP expression

    Depositor

    Pierre Vanderhaeghen

    Article

    Suzuki et al Cell. 2018 May 31;173(6):1370-1384.e16.

    Type

    Empty backbone

    Use

    Lentiviral

    Available Since

    May 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. Cerulean

    Plasmid

    #15214

    Depositor

    Dave Piston

    Article

    Rizzo et al Nat Biotechnol. 2004 Apr . 22(4):445-9.

    Insert

    monomeric Cerulean
    

    Expression

    Mammalian

    Mutation

    A206K

    Available Since

    June 22, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMK286 (mAID-NeoR)

    Plasmid

    #72824

    Purpose

    C-terminal tagging with mAID using CRISPR/Cas9

    Depositor

    Masato Kanemaki

    Article

    Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

    Insert

    mAID-Neo
    

    Use

    CRISPR

    Tags

    mAID

    Expression

    Mammalian

    Available Since

    March 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGL2- Empty control -SV40-Luc

    Plasmid

    #26280

    Purpose

    Addgene next-generation sequencing identified numerous modifications from the indicated plasmid backbone. Please see the sequences link for additional information.

    Depositor

    Rudolf Jaenisch

    Article

    Hanna et al Proc Natl Acad Sci U S A. 2010 May 18. 1

    Type

    Empty backbone

    Use

    Luciferase

    Expression

    Mammalian

    Available Since

    Oct. 20, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCIOX

    Plasmid

    #51300

    Purpose

    E.coli expression vector for His8-SUMO tagged recombinant protein

    Depositor

    Andrea Mattevi

    Type

    Empty backbone

    Tags

    His8 + SUMO

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 28, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. DsRed-rab7 DN

    Plasmid

    #12662

    Depositor

    Richard Pagano

    Article

    Choudhury et al J Clin Invest. 2002 Jun . 109(12):1541-5

    Insert

    RAB7 (Homo sapiens) (RAB7A Human)
    

    Tags

    DsRed and HA

    Expression

    Mammalian

    Mutation

    T22N

    Available Since

    Oct. 16, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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18. EB3-mNeonGreen

    Plasmid

    #98881

    Purpose

    In vivo visualization of microtuble plus ends (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

    Insert

    EB3
    

    Tags

    mNeonGreen

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. Cdk5-HA

    Plasmid

    #1872

    Depositor

    Sander van den Heuvel

    Article

    van den Heuvel et al Science 1993 Dec 24;262(5142):2050-4.

    Insert

    Cdk5 (CDK5 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    April 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMi(3xP3-EGFP)

    Plasmid

    #102540

    Purpose

    Transgenesis vector based on the Minos transposon, carrying the 3xP3-EGFP marker

    Depositor

    Michalis Averof, Charalambos Savakis

    Article

    Pavlopoulos et al Genetics. 2004 Jun;167(2):737-46.

    Insert

    Minos transpoable element with 3xP3-EGFP marker
    

    Available Since

    March 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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