164,577 results

1. pPGK-GZF1-RR

   Plasmid

   #35092

   Depositor

   David Segal

   Article

   Bhakta et al Methods Mol Biol. 2010;649:3-30.

   Insert

   ZFN GZF1-Fok(RR)
   

   Use

   Tags

   HA and SV40 NLS

   Expression

   Mammalian

   Mutation

   contains the "RR" obligate heterodimer …

   Promoter

   PGK

   Available Since

   May 10, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-CAG-Cre-3xmiR122-WPRE-HGHpA

   Plasmid

   #183776

   Purpose

   AAV genome with a CAG driven Cre recombinase with mR122 target sequence repeats to reduce transgene expression in hepatocytes.

   Depositor

   Ben Deverman

   Article

   Krolak et al Nat Cardiovasc Res. 2022 Apr;1(4):389-40

   Insert

   Cre
   

   Use

   AAV

   Tags

   Expression

   Mutation

   NA

   Promoter

   CAG

   Available Since

   May 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV_CMV::V5-EGFR

   Plasmid

   #199717

   Purpose

   Expresses V5-tagged EGFR in mammalian cells.

   Depositor

   Alice Ting

   Article

   Roh et al J Am Chem Soc. 2023 May 10;145(18):10220

   Insert

   V5-EGFR
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   April 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDY32 AAVS1-PuroR-9xTet0-minCMV-IGKleader-hIgG1_FC-Myc-PDGFRb-T2A-Citrine-PolyA

   Plasmid

   #161928

   Purpose

   Donor plasmid for integrating minCMV promoter-driven dual surface marker/fluorescent reporter at AAVS1 locus

   Depositor

   Michael Bassik, Lacra Bintu

   Article

   Tycko et al Cell. 2020 Dec 9. pii: S0092-8674(20)315

   Insert

   Surface marker and Citrine activation reporter
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   pEF

   Available Since

   Feb. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pKIR1.1

   Plasmid

   #85758

   Purpose

   CRISPR/Cas9 in Arabidopsis with seed a fluorescent reporter

   Depositor

   Tetsuya Higashiyama

   Article

   Tsutsui et al Plant Cell Physiol. 2016 Nov 17. pii: pc

   Insert

   human-codon-optimized SpCas9
   

   Use

   CRISPR

   Tags

   FLAG

   Expression

   Plant

   Mutation

   Promoter

   AtRPS5A

   Available Since

   Jan. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. ADORA2B-DuET

   Plasmid

   #213175

   Purpose

   The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

   Depositor

   Thomas Sakmar

   Article

   Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

   Insert

   ADORA2B (ADORA2B Human)
   

   Use

   Tags

   1D4 and FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV, T7

   Available Since

   April 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGIPZ-PD-L1-EGFP

   Plasmid

   #120933

   Purpose

   Express PD-L1-EGFP fusion protein in mammalian cells

   Depositor

   Mien-Chie Hung

   Article

   Yang et al Cell Res. 2018 Aug;28(8):862-864. doi: 1

   Insert

   PD-L1-EGFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Feb. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. Lyso-B

   Plasmid

   #209873

   Purpose

   Dimerization dependent fluorescent protein B anchored to cytosolic face of the lysosomal membrane with human LAMP1

   Depositor

   Sarah Cohen

   Article

   Miner et al Contact (Thousand Oaks). 2024 Feb 4;7:25

   Insert

   ddGFP B
   

   Use

   Tags

   LAMP1

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Nov. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAT009

   Plasmid

   #171635

   Purpose

   Plasmid containing a U6 promoter expressing a spyCas9 sgRNA targeting B2M and CAG promoter expressing mTagBFP2

   Depositor

   Jennifer Doudna

   Article

   Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

   Inserts

   spyCas9 sgRNA-B2M
   mTagBFP2
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG and U6

   Available Since

   June 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTRIP-CMV-mCherry-53BP1

    Plasmid

    #127658

    Purpose

    Over-express mCherry-53BP1 fragment to monitor 53BP1 foci.

    Depositor

    Nicolas Manel

    Article

    Gentili et al Cell Rep. 2019 Feb 26;26(9):2377-2393.e1

    Insert

    53BP1 (TP53BP1 Human)
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mutation

    Nucleotides 3658-5133 of TP53BP1 (NM_005657.2) fu…

    Promoter

    Available Since

    July 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. plix403-V5-N-mEGFP

    Plasmid

    #223957

    Purpose

    Gateway cloning of genes of interest with an N-terminal V5-mEGFP fusion; generation of stable cells with doxycycline inducible expression

    Depositor

    Roko Žaja, Karla Feijs

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    V5-mEGFP

    Expression

    Mutation

    Promoter

    Available Since

    Oct. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pΔΦ

    Plasmid

    #60009

    Purpose

    Deleted foamy virus vector plasmid containing a backbone with a polylinker region for insertion of a transgene cassette. Ampicillin resistant.

    Depositor

    David Russell

    Article

    Trobridge et al Mol Ther. 2002 Sep;6(3):321-8.

    Type

    Empty backbone

    Use

    Deleted foamy virus

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available Since

    June 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHH0103_GNAI2_G-alpha

    Plasmid

    #109791

    Purpose

    Protein expression and purification of GNAI2_G-alpha

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

    Insert

    GNAI2_G-alpha (GNAI2 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGL4.0-TERT WT

    Plasmid

    #84924

    Purpose

    luciferase reporter for TERT promoter

    Depositor

    Joseph Costello

    Article

    Bell et al Science. 2015 May 29;348(6238):1036-9. d

    Insert

    TERT promoter (TERT Human)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    TERT

    Available Since

    March 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. AP1muA-Halo-ALFA-polyA-G418 HR

    Plasmid

    #229681

    Purpose

    Homology repair plasmid for endogenous tagging of AP1muA at the C-terminus with HaloTag and an ALFA tag. Contains a G418 resistance cassette for selection of edited cells.

    Depositor

    Francesca Bottanelli

    Article

    Stockhammer et al Nat Cell Biol. 2024 Oct 4. doi: 10.1038/

    Insert

    genomic sequence AP1M1 locus (AP1M1 Human)
    

    Use

    CRISPR

    Tags

    Halo, ALFA

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. strep-IRF8

    Plasmid

    #102872

    Purpose

    Express strep-tagged mouse IRF8 protein in E. coli

    Depositor

    Gary Stormo

    Article

    Chang et al BMC Mol Biol. 2018 Mar 27;19(1):5. doi:

    Insert

    IRF8 (Irf8 Mouse)
    

    Use

    Tags

    strep tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    April 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. Rtn3L-mNeonGreen

    Plasmid

    #169034

    Purpose

    Express human Rtn3L in mammalian cells

    Depositor

    Gia Voeltz

    Article

    Wu et al Dev Cell. 2021 Jan 11;56(1):52-66.e7. do

    Insert

    Rtn3L (RTN3 Human)
    

    Use

    Tags

    mNeonGreen

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDN77

    Plasmid

    #61347

    Purpose

    mCherry fused to LINuS, a light-inducible nuclear localization signal (biNLS2/IkBα NES)

    Depositor

    Roland Eils, Barbara Di Ventura

    Article

    Niopek et al Nat Commun. 2014 Jul 14;5:4404. doi: 10.

    Insert

    mCherry
    

    Use

    Tags

    GS linker-AsLov2-biNLS2 and NES IkBa

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLenti-RIGI-Apex- T2A-EGFP

    Plasmid

    #167288

    Purpose

    Lentiviral expression plasmid encoding an APEX2 RIG-I fusion product in frame with a self-cleaving eGFP. APEX biotin ligase can biotynilate RNAs in proximity of the RLR RIG-I sensor.

    Depositor

    Agnel Sfeir

    Article

    Tigano et al Nature. 2021 Mar;591(7850):477-481. doi:

    Insert

    DDX58 (RIGI Human)
    

    Use

    Lentiviral

    Tags

    APEX2 and EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pB1H2

    Plasmid

    #12613

    Depositor

    Scot Wolfe

    Article

    Meng et al Nat Biotechnol. 2005 Aug . 23(8):988-94.

    Insert

    dorsal RHR in pB1H2 (dl Fly)
    

    Use

    Tags

    flag

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Sept. 26, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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21. pcDNA3.1/hChR2(H134R)-EYFP

    Plasmid

    #20940

    Purpose

    Mammalian expression of humanized ChR2 with H134R mutation fused to EYFP for optogenetic activation

    Depositor

    Karl Deisseroth

    Article

    Zhang et al Nature. 2007 Apr 5. 446(7136):633-9.

    Insert

    channelrhodopsin-2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    H134R mutation in ChR2

    Promoter

    CMV

    Available Since

    May 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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22. pTreTight-Htt94Q-CFP

    Plasmid

    #23966

    Depositor

    Nico Dantuma

    Article

    Maynard et al Proc Natl Acad Sci U S A. 2009 Aug 18. 1

    Insert

    N-terminal fragment Huntingtin Q94 (HTT Human)
    

    Use

    Tags

    CFP

    Expression

    Mammalian

    Mutation

    94 polyQ repeat

    Promoter

    Available Since

    April 15, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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23. MCP-YFP

    Plasmid

    #101160

    Purpose

    to express MCP-YFP fusion protein

    Depositor

    Mazhar Adli

    Article

    Qin et al Nat Commun. 2017 Mar 14;8:14725. doi: 10

    Insert

    MCP-YFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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24. pJMP3665

    Plasmid

    #222351

    Purpose

    Expression vector with PabstBR promoter, hygR marker, and replicates in A. baumannii and E. coli; empty vector plasmid with MCS for cloning - we recommend NcoI/BamHI.

    Depositor

    Jason Peters

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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25. pHR-HNRNPA1C-mCh-Cry2WT

    Plasmid

    #101226

    Purpose

    hnRNPA1(186-320) fused to mCh-Cry2WT

    Depositor

    Clifford Brangwynne

    Article

    Shin et al Cell. 2017 Jan 12;168(1-2):159-171.e14.

    Insert

    hnRNPA1(186-320) (HNRNPA1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available Since

    Feb. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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26. MIB2-FLAG

    Plasmid

    #37117

    Depositor

    Martin Dorf

    Article

    Li et al Immunity. 2011 Sep 23;35(3):426-40. Epub

    Insert

    MIB2, mindbomb E3 ubiquitin protein ligase 2 (MIB2 Human)
    

    Use

    Tags

    3x FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 3, 2012

    Availability

    Academic Institutions and Nonprofits only

27. mTagBFP-Nucleus-7

    Plasmid

    #55265

    Purpose

    Localization: Nuclear Localization, Excitation: 399, Emission: 456

    Depositor

    Michael Davidson

    Insert

    SV40 T-Antigen Nuclear Localization Signal (NLS)
    

    Use

    Tags

    mTagBFP-NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 22, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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28. PresentER-Cassette GFP

    Plasmid

    #102942

    Purpose

    PresentER with removable cassette for cloning of MHC-I antigen (GFP version)

    Depositor

    David Scheinberg

    Article

    Gejman et al Elife. 2018 Nov 30;7. pii: 41090. doi: 1

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    LTR

    Available Since

    April 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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29. pENTR_L1-YFP-Turbo-L2

    Plasmid

    #127350

    Purpose

    gateway entry vector for making N-terminal YFP-TurboID fusions with nuclear and non-nuclear proteins

    Depositor

    Dominique Bergmann

    Article

    Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

    Insert

    TurboID (BirA mutant)
    

    Use

    Gateway entry vector

    Tags

    GS linker, V5, and YFP

    Expression

    Mutation

    Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

    Promoter

    no promoter

    Available Since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pNP1950

    Plasmid

    #222729

    Purpose

    J23119-BsaI_bRNA Golden Gate Landing Pad-HDV; T7-IS621 Recombinase

    Depositor

    Patrick Hsu

    Article

    Durrant et al Nature. 2024 Jun;630(8018):984-993. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    July 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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