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pGL3con-miR15aPer

Plasmid

#21116

Depositor

Joshua Mendell

Article

Hwang et al Proc Natl Acad Sci U S A. 2009 Apr 28;10

Insert

hsa-miR-15a sensor

Expression

Mammalian

Available Since

May 13, 2009

Availability

Academic Institutions and Nonprofits only

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EF1a_SIX1_P2A_Hygro_Barcode

Plasmid

#120476

Purpose

Barcoded lentiviral vector to express SIX1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

SIX1 (SIX1 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 6, 2019

Availability

Academic Institutions and Nonprofits only

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YY120: pPB_TRE::GEMINI(B)-p2A-GEMINI(A)

Plasmid

#228881

Purpose

PiggyBac plasmid expressing the two GEMINI building blocks (A and B) in an equimolar ratio under the control of a doxycycline-inducible promotor.

Depositor

Dingchang Lin

Article

Yan et al Nature. 2026 Mar 3. doi: 10.1038/s41586-

Insert

Building blocks of GEMINI for intracellular assembly

Expression

Mammalian

Promoter

TRE

Available Since

Feb. 3, 2026

Availability

Academic Institutions and Nonprofits only

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pCMV-HA_N-mouse Bcl6

Plasmid

#138410

Purpose

Express mouse Bcl6 in mammalian cells

Depositor

Bruce Beutler

Article

Misawa et al Sci Immunol. 2020 Jan 24;5(43):eaaz0085.

Insert

B cell lymphoma 6 (Bcl6 Mouse)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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pKI_ΩBBMVP16&SRDXWUSm1

Plasmid

#220349

Purpose

Expresses BBM-VP16 and SRDX-WUSm1 in plant cells

Depositor

Tomoko Igawa

Article

Sato et al Front Plant Sci. 2024 Apr 3;15:1308417.

Inserts

BABY BOOM (BBM Mustard Weed)
WUSCHEL (WUS Mustard Weed)

Tags

Superman Repression Domain X (SRDX) and VP16 tran…

Expression

Plant

Mutation

two amino acid substitution in WUS-box: L255A L25…

Promoter

CaMV 35S and RPS5A

Available Since

July 29, 2024

Availability

Academic Institutions and Nonprofits only

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pMOS010: CheRiff-eGFP channelrhodopsin (plasma membrane)

Plasmid

#163044

Purpose

CheRiff-eGFP channelrhodopsin (plasma membrane)

Depositor

Adam Cohen

Article

Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

Insert

CheRiff

Use

Lentiviral

Tags

eGFP

Expression

Mammalian

Promoter

CMV

Available Since

April 22, 2021

Availability

Academic Institutions and Nonprofits only

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pBEAST-pBen-sfGFP

Plasmid

#114598

Purpose

"Output expression of sfGFP under the activation of BenR transcription factor for cell-free expression"

Depositor

Jerome Bonnet

Article

Voyvodic et al Nat Commun. 2019 Apr 12;10(1):1697. doi:

Insert

sfGFP

Use

Synthetic Biology; Cell-free protein synthesis

Promoter

PBen

Available Since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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EF1a_PAX7_P2A_Hygro_Barcode

Plasmid

#120472

Purpose

Barcoded lentiviral vector to express PAX7 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

PAX7 (PAX7 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pDB-hPER2-mScarlet-I-CD4-bla

Plasmid

#179449

Purpose

Donor vector to knock in mScarlet-I C-terminal to human PER2 gene

Depositor

Achim Kramer

Article

Gabriel et al Nat Commun. 2021 Jun 18;12(1):3796. doi:

Insert

mScarletI

Use

CRISPR

Tags

His/Flag

Promoter

None

Available Since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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CRISPR_TRAC

Plasmid

#164993

Purpose

Expression of gRNA targeting TCRalpha constant locus

Depositor

Matthew Meyerson

Article

Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

Insert

gRNA against TRAC

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

March 19, 2021

Availability

Academic Institutions and Nonprofits only

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pBEAST-BenR

Plasmid

#114597

Purpose

"Strong constitutive expression of transcription factor, BenR, for cell-free expression"

Depositor

Jerome Bonnet

Article

Voyvodic et al Nat Commun. 2019 Apr 12;10(1):1697. doi:

Insert

BenR

Use

Synthetic Biology; Cell-free protein synthesis

Promoter

OR2-OR1-Pr

Available Since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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V2-MESA-35F-M-dCas9

Plasmid

#84506

Purpose

MESA target chain with V2-MESA ectodomain, 35 extracellular linkers, a flag tag, M cleavage sequence, and dCas9-VP64

Depositor

Joshua Leonard

Article

Schwarz et al Nat Chem Biol. 2016 Dec 12. doi: 10.1038

Insert

V2-MESA-35F-M-dCas9

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pGC629

Plasmid

#71364

Purpose

proximal somatic gonad expression of DAF-16 in C.elegans

Depositor

Jane Hubbard

Article

Qin et al Nat Commun. 2015 May 11;6:7107. doi: 10.

Insert

daf-16 (daf-16 Nematode)

Tags

GFP(65C)

Expression

Worm

Promoter

fos1a promoter

Available Since

Dec. 8, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA1-Bystin

Plasmid

#11869

Depositor

Michiko Fukuda

Article

Suzuki et al Proc Natl Acad Sci U S A. 1998 Apr 28. 9

Insert

Bystin (BYSL Human)

Expression

Mammalian

Available Since

Sept. 1, 2006

Availability

Academic Institutions and Nonprofits only
SNAPtag-PTS1

Plasmid

#182883

Purpose

Express SNAPtag-PTS1 in mammalian cells. SNAPtag targets to peroxisomes

Depositor

Pekka Katajisto

Article

Bui et al Nat Commun. 2025 Apr 26;16(1):3932. doi:

Insert

SNAPtag-PTS1

Use

Lentiviral

Tags

SNAPtag

Expression

Mammalian

Mutation

no

Promoter

CMV

Available Since

April 28, 2025

Availability

Academic Institutions and Nonprofits only

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PLX306 iCre tetO NICD rtTA

Plasmid

#158049

Purpose

lenti-viral construct with Cre recombinase and doxycycline inducible expression of mouse Notch intracellular domain

Depositor

Daniel Schramek

Article

Loganathan et al Science. 2020 Mar 13;367(6483):1264-1269

Insert

mouse Notch Intra Cellular Domain

Tags

V5

Expression

Mammalian

Promoter

tetO promoter

Available Since

Oct. 5, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pTXB1-nbMmKappa-DddA11_NT

Plasmid

#232442

Purpose

Anti-mouse kappa chain specific nanobody-DddA11_NT for D&Dseq (sc TFs molecular footprint)

Depositor

Ivan Raimondi

Article

Chi et al bioRxiv [Preprint]. 2025 Jan 2:2024.12.3

Insert

nbMmKappa-DddA11_NT

Available Since

Feb. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pColE1_70a_deGFP_AmpR

Plasmid

#119161

Purpose

Expresses deGFP from a strong sigma 70 promoter

Depositor

Vincent Noireaux

Article

Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

Insert

deGFP

Use

Crispr

Available Since

Dec. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pBabe-TetCMV-puro-mVenus-PA-Rac1-C450A

Plasmid

#22023

Depositor

Klaus Hahn

Article

Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

Insert

mVenus-PA-Rac1-C450A (RAC1 Human, Avena sativa (oat))

Use

Retroviral

Expression

Mammalian

Mutation

The LOV domain contains the mutation C450A.Rac1 s…

Available Since

Sept. 29, 2009

Availability

Academic Institutions and Nonprofits only
pLentiCrisprV2 sgRNA hCRY1 c-term

Plasmid

#179453

Purpose

Lentiviral Crispr/Cas9 plasmid targeting hCRY1 at C-terminus to generate C-terminal knock-in

Depositor

Achim Kramer

Article

Gabriel et al Nat Commun. 2021 Jun 18;12(1):3796. doi:

Insert

sgRNA targeting human CRY1

Use

CRISPR and Lentiviral

Promoter

hU6

Available Since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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