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Showing: 7881 - 7900 of 8705 results

  3. FB003

    Plasmid
    #119677
    Purpose
    Transcriptional Unit (TU) for hygromicin resistance in fungi obtained by combining FB001+GB0211+FB002 into pDGB3alpha2. According to FungalBraid/GoldenBraid modular DNA assembly for ATMT
    Depositor
    Jose Marcos
    Article
    Hernanz-Koers et al Fungal Genet Biol. 2018 Jul;116:51-61. d
    Insert
    PtrpC:hph:Ttub
    Use
    Synthetic Biology
    Available Since
    April 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    119677-plasmid-map-sequence-id-238439

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  4. pAM4663

    Plasmid
    #85126
    Purpose
    NSIII -Cm-PkaiB-luc+ (firefly) vector constructed by seamless cloning with Cm casette codon optimized for S7942. PkaiB-luc+ amplified from pAM2105.
    Depositor
    Susan Golden
    Article
    Chang et al Science. 2015 Jul 17;349(6245):324-8. do
    Insert
    PkaiB-luciferase
    Expression
    Bacterial
    Available Since
    May 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    85126-plasmid-map-sequence-id-180127

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  5. pAS_4xG1 PylT A41AA C55A sfGFP 150TAG

    Plasmid
    #154770
    Purpose
    plasmid with 4xG1 PylT cassette (G1 PylT mutant A41AA C55A) and amber suppression reporter sfGFP 150 TAG stop, for transient transfection or stable, piggybac-mediated, integration
    Depositor
    Simon Elsaesser
    Article
    Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi
    Insert
    sfGFP
    Expression
    Mammalian
    Mutation
    150TAG in GFP reporter, G1 PylT with A41AA and C5…
    Promoter
    EF1
    Available Since
    Oct. 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    154770-plasmid-map-sequence-id-309867

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  10. pET151-Vps4(101-437)-Hcp1 K321A/R325A

    Plasmid
    #89287
    Purpose
    Bacterial expression of S. cerevisiae Vps4 AAA ATPase cassette fused to P. aeruginosa Hcp1
    Depositor
    Chris Hill
    Article
    Monroe et al Elife. 2017 Apr 5;6. pii: e24487. doi: 1
    Insert
    Vacuolar protein sorting-associated protein 4 (VPS4 Budding Yeast)
    Tags
    6xHis-V5-TEV and Hcp1
    Expression
    Bacterial
    Mutation
    deleted amino acids 1-100, mutated Lys-321 and Ar…
    Promoter
    T7
    Available Since
    April 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    89287-plasmid-map-sequence-id-175359

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  14. pMDC32B-BS-AtMIR390a-A18G-B/c

    Plasmid
    #246716
    Purpose
    Cloning amiRNA sequences in AtMIR390a-A18G precursors for in planta expression
    Depositor
    Alberto Carbonell
    Article
    Llorens-Gamez et al Plant J. 2026 Jan;125(1):e70665. doi: 10
    Insert
    Basal stem of Arabidopsis MIR390a precursor with A18G mutation & chloramphenicol-ccdB cassette flanked by two BsaI sites (MIR390a Mustard Weed)
    Use
    RNAi
    Expression
    Plant
    Available Since
    Jan. 14, 2026
    Availability
    Academic Institutions and Nonprofits only
    246716-plasmid-map-sequence-id-494273

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  15. pEvolvR-mCherry-Sniper-nCas9(D10A)-PolI5MΔ

    Plasmid
    #249071
    Purpose
    Expresses nucleus-localized Sniper-nCas9 (D10A) fused to PolI5MΔ and mCherry using a CMV promoter. Includes a GFP cassette flanked by BsmbI cut sites to knock in and coexpress user-defined gRNAs.
    Depositor
    John Dueber, David Schaffer
    Article
    Hurtado et al Nat Commun. 2025 Apr 19;16(1):3723. doi:
    Insert
    Sniper-nCas9-PolI5MΔ
    Use
    CRISPR
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    deletion of PolI5M N-terminal flap endonuclease d…
    Promoter
    CMV
    Available Since
    Jan. 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    249071-plasmid-map-sequence-id-496323

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  16. pEvolvR-mCherry-SuperFi-nCas9(D10A)-PolI5MΔ

    Plasmid
    #249070
    Purpose
    Expresses nucleus-localized SuperFi-nCas9 (D10A) fused to PolI5MΔ and mCherry using a CMV promoter. Includes a GFP cassette flanked by BsmbI cut sites to knock in and coexpress user-defined gRNAs.
    Depositor
    John Dueber, David Schaffer
    Article
    Hurtado et al Nat Commun. 2025 Apr 19;16(1):3723. doi:
    Insert
    SuperFi-nCas9-PolI5M?
    Use
    CRISPR
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    deletion of PolI5M N-terminal flap endonuclease d…
    Promoter
    CMV
    Available Since
    Jan. 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    249070-plasmid-map-sequence-id-496322

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  17. pEvolvR-mCherry-enCas9(D10A)-PolI5MΔ

    Plasmid
    #249068
    Purpose
    Expresses nucleus-localized enCas9 (D10A) fused to PolI5MΔ and mCherry using a CMV promoter. Includes a GFP cassette flanked by BsmbI cut sites to knock in and coexpress user-defined gRNAs.
    Depositor
    John Dueber, David Schaffer
    Article
    Hurtado et al Nat Commun. 2025 Apr 19;16(1):3723. doi:
    Insert
    enCas9-PolI5MΔ
    Use
    CRISPR
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    deletion of PolI5M N-terminal flap endonuclease d…
    Promoter
    CMV
    Available Since
    Jan. 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    249068-plasmid-map-sequence-id-496320

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  18. pEvolvR-mCherry-HSC1.2-nCas9(D10A)-PolI5MΔ

    Plasmid
    #249073
    Purpose
    Expresses nucleus-localized HSC1.2-nCas9 (D10A) fused to PolI5MΔ and mCherry using a CMV promoter. Includes a GFP cassette flanked by BsmbI cut sites to knock in and coexpress user-defined gRNAs.
    Depositor
    John Dueber, David Schaffer
    Article
    Hurtado et al Nat Commun. 2025 Apr 19;16(1):3723. doi:
    Insert
    HSC1.2-nCas9-PolI5MΔ
    Use
    CRISPR
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    deletion of PolI5M N-terminal flap endonuclease d…
    Promoter
    CMV
    Available Since
    Jan. 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    249073-plasmid-map-sequence-id-496319

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  19. pEvolvR-mCherry-LZ3-nCas9(D10A)-PolI5MΔ

    Plasmid
    #249072
    Purpose
    Expresses nucleus-localized LZ3-nCas9 (D10A) fused to PolI5MΔ and mCherry using a CMV promoter. Includes a GFP cassette flanked by BsmbI cut sites to knock in and coexpress user-defined gRNAs.
    Depositor
    John Dueber, David Schaffer
    Article
    Hurtado et al Nat Commun. 2025 Apr 19;16(1):3723. doi:
    Insert
    LZ3-nCas9-PolI5MΔ
    Use
    CRISPR
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    deletion of PolI5M N-terminal flap endonuclease d…
    Promoter
    CMV
    Available Since
    Jan. 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    249072-plasmid-map-sequence-id-496318

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  20. pEvolvR-mCherry-HF1-nCas9(D10A)-PolI5MΔ

    Plasmid
    #249075
    Purpose
    Expresses nucleus-localized HF1-nCas9 (D10A) fused to PolI5MΔ and mCherry using a CMV promoter. Includes a GFP cassette flanked by BsmbI cut sites to knock in and coexpress user-defined gRNAs.
    Depositor
    John Dueber, David Schaffer
    Article
    Hurtado et al Nat Commun. 2025 Apr 19;16(1):3723. doi:
    Insert
    HF1-nCas9-PolI5MΔ
    Use
    CRISPR
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    deletion of PolI5M N-terminal flap endonuclease d…
    Promoter
    CMV
    Available Since
    Jan. 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    249075-plasmid-map-sequence-id-496315

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Showing: 7881 - 7900 of 8705 results