We narrowed to 15,506 results for: LIS

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pCDH-V3-CD20-Puro

Plasmid

#209757

Purpose

Lentiviral transfer plasmid to express the 5'-UTR and CDS of the human CD20 gene, MS4A1. Specifically, the variant 3 mRNA isoform.

Depositor

Andrei Thomas-Tikhonenko

Article

Ang et al Blood. 2023 Sep 8:blood.2023020400. doi:

Insert

MS4A1 (MS4A1 Human)

Use

Lentiviral

Available Since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pKHH030_sgCtrl#1

Plasmid

#174142

Purpose

Lentiviral vector expressing a control sgRNA that targets a safe harbor site

Depositor

Julien Sage

Article

Chaikovsky et al Nature. 2021 Apr;592(7856):794-798. doi:

Insert

Control sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

Sept. 13, 2021

Availability

Academic Institutions and Nonprofits only

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lentiCRISPRv2_AMBRA1#1

Plasmid

#174152

Purpose

Lentiviral vector expressing Cas9 and a sgRNA against the human AMBRA1 gene

Depositor

Julien Sage

Article

Chaikovsky et al Nature. 2021 Apr;592(7856):794-798. doi:

Insert

AMBRA (AMBRA1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

Sept. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR 223 VPS4A

Plasmid

#169319

Purpose

Gateway entry vector for human VPS4A ORF

Depositor

Francisca Vazquez

Article

Neggers et al Cell Rep. 2020 Dec 15;33(11):108493. doi

Insert

VPS4A (VPS4A Human)

Use

Gateway Cloning

Promoter

NA

Available Since

May 11, 2021

Availability

Academic Institutions and Nonprofits only

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FLAG-PRMT3(E338Q)

Plasmid

#164696

Purpose

Expression of PRMT3(E338Q) with an N-terminal FLAG tag

Depositor

Cheryl Arrowsmith

Article

Kaniskan et al Angew Chem Int Ed Engl. 2015 Apr 20;54(1

Insert

PRMT3 (PRMT3 Human)

Tags

FLAG

Expression

Mammalian

Mutation

E338Q

Promoter

CMV

Available Since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-TadCBEd-SpG-P2A-EGFP (BKS777/AHK571)

Plasmid

#223124

Purpose

pCMV and pT7 Human expression plasmid for TadCBEd-SpG with C-term BPNLS-P2A-EGFP

Depositor

Benjamin Kleinstiver

Article

Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

Insert

human codon optimized TadCBEd-SpG-2xUGI-P2A-EGFP

Use

CRISPR

Tags

2xUGI-BPNLS-P2A-EGFP and BPNLS-TadA-CDd

Expression

Mammalian

Mutation

TadA-CDd mutations; nSpG=D10A/D1135L/S1136W/G1218…

Promoter

CMV and T7

Available Since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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pUC19-U6-BsmBI_epegRNA_entry-mpknot_(LM1140)

Plasmid

#228253

Purpose

pU6 entry plamid for cloning prime editor mpknot epegRNAs (requires BsmBI or Esp3I digest and ligation of duplexed oligos)

Depositor

Benjamin Kleinstiver

Article

Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

Insert

mpknot entry plasmid backbone for epegRNA expression via human U6 promoter

Expression

Mammalian

Mutation

n/a

Promoter

U6

Available Since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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pU6_rd6_pegRNA_(PP7-C4-Q1)

Plasmid

#232433

Purpose

pegRNA with optimized 3' modifications to correct the rd6 mutation

Depositor

Gil Westmeyer

Article

Geilenkeuser et al Cell. 2025 May 15;188(10):2637-2655.e31.

Insert

3'-PP7-tagged pegRNA for rd6 correction driven by human U6 promoter

Use

CRISPR

Promoter

U6

Available Since

April 10, 2025

Availability

Academic Institutions and Nonprofits only

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pQLL-Mariner2_AG

Plasmid

#217063

Purpose

Donor plasmid of Mariner2_AG Transposon

Depositor

Haoyi Wang

Article

Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

Insert

TIRs of Mariner2_AG transposon

Expression

Mammalian

Available Since

Dec. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pMV-SB100X[EF1α-copGFP]

Plasmid

#218323

Purpose

Donor plasmid of SB100X Transposon with copGFP mammalian expression cassette

Depositor

Haoyi Wang

Article

Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

Insert

copGFP mammalian expression cassette

Expression

Mammalian

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-HAT1_AG

Plasmid

#217113

Purpose

Mammalian expression vector for expression of HAT1_AG transposase (CMV promoter)

Depositor

Haoyi Wang

Article

Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

Insert

HAT1_AG transposase

Expression

Mammalian

Available Since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pNK5712

Plasmid

#219752

Purpose

pGAP-like vector encoding mutant of Neonothopanus nambi hispidin-3-hydroxylase nnH3H_v2 under control of GAP promoter, for yeast expression

Depositor

Alexander Mishin

Article

Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

Insert

pGAP - nnH3H_v2 - tAOX

Expression

Yeast

Available Since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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CL20_mEGFP_TMPRSS2_ETV4

Plasmid

#205945

Purpose

Express mEGFP-tagged fusion protein, TMPRSS2_ETV4 from patient-derived sequence

Depositor

Richard Kriwacki

Article

Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

Insert

TMPRSS2_ETV4 (TMPRSS2, ETV4 Human)

Tags

mEGFP

Expression

Mammalian

Available Since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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B*40:01:01

Plasmid

#203257

Purpose

Overexpress HLA allele

Depositor

Derin Keskin, Catherine Wu

Article

Sarkizova et al Nat Biotechnol. 2020 Feb;38(2):199-209.

Insert

B*40:01:01 (HLA-B Human)

Expression

Mammalian

Available Since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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B*57:03:01

Plasmid

#203260

Purpose

Overexpress HLA allele

Depositor

Derin Keskin, Catherine Wu

Article

Sarkizova et al Nat Biotechnol. 2020 Feb;38(2):199-209.

Insert

B*57:03:01 (HLA-B Human)

Expression

Mammalian

Available Since

July 7, 2023

Availability

Academic Institutions and Nonprofits only

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pCBh_3x Flag-NLS-OsCas12f1-NLS_pA_phU6_gRNA scaffold-spacer_pCMV_mCherry_pA

Plasmid

#197024

Purpose

Vector encoding a human codon-optimized OsCas12f1 driven by CBh promoter, guide RNAs compatible with OsCas12f1 driven by hU6, and mCherry driven by CMV promoter

Depositor

Xiangfeng Kong

Article

Kong et al Nat Commun. 2023 Apr 11;14(1):2046. doi:

Insert

humanized OsCas12f1

Expression

Mammalian

Promoter

CBh, CMV, hU6

Available Since

May 8, 2023

Availability

Academic Institutions and Nonprofits only

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pIG-721 CTLA4-GFP T124P

Plasmid

#186126

Purpose

CTLA4 gene knockin with mutation

Depositor

Alexander Marson

Article

Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

Insert

CTLA4 (CTLA4 Human)

Use

CRISPR

Mutation

T124P

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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pIG-703pUC19-tNGFR-P2A-BACH2

Plasmid

#186112

Purpose

Knockin of truncated NGFR to target gene

Depositor

Alexander Marson

Article

Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

Insert

BACH2 (BACH2 Human)

Use

CRISPR

Mutation

WT with tNGFR sequence

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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pTR-340 pUC19-tNGFR-P2A-IL2RG N

Plasmid

#186067

Purpose

Knockin of truncated NGFR to target gene

Depositor

Alexander Marson

Article

Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

Insert

IL2RG (IL2RG Human)

Use

CRISPR

Mutation

WT with tNGFR sequence

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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pX028

Plasmid

#167151

Purpose

MoClo-compatible Level 1 (position 2) vector encoding Neonothopanus nambi hispidin synthase nnHispS under control of 0.4 kb 35S promoter, for expression in plants

Depositor

Karen Sarkisyan

Article

Mitiouchkina et al Nat Biotechnol. 2020 Apr 27. pii: 10.103

Insert

p35s-nnHispS-ocsT

Expression

Plant

Promoter

p35s

Available Since

June 21, 2021

Availability

Academic Institutions and Nonprofits only

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