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pSLQ5080_pHR_PGK_sfGFP_CoV-F2

Plasmid

#155304

Purpose

SARS-CoV-2 fluorescent reporter 2

Depositor

Stanley Qi

Article

Abbott et al Cell. 2020 May 14;181(4):865-876.e12. do

Insert

Superfolder GFP fused with SARS-CoV-2 fluorescent reporter 2 (N, ORF1ab Synthetic)

Use

Lentiviral

Expression

Mammalian

Promoter

PGK

Available Since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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cytosolic moPrP (3F4)

Plasmid

#1321

Depositor

Susan Lindquist

Article

Ma et al Proc Natl Acad Sci U S A 2001 Dec 18;98(

Insert

PrP (Prnp Mouse)

Expression

Mammalian

Mutation

N-term truncation (to make gene cytosolic), vario…

Available Since

Jan. 21, 2005

Availability

Academic Institutions and Nonprofits only
pX330-NL-DHFR-SpCas9

Plasmid

#124522

Purpose

Expresses SpCas9 fused to DHFR domains on both the N-terminus and an internal loop in mammalian cells

Depositor

Amit Choudhary

Article

Manna et al Angew Chem Int Ed Engl. 2019 May 6;58(19

Insert

NL-DHFR-SpCas9

Use

AAV

Tags

Destabilized domain of E.coli dihydrofolate reduc…

Expression

Mammalian

Promoter

Cbh

Available Since

July 19, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-DIO-ChroME-ST-P2A-H2B-mRuby3

Plasmid

#170161

Purpose

Cation channelrhodopsin ChroME targeted to the neuronal soma and proximal dendrites and separated from nuclear mRuby3 by P2A in a viral vector

Depositor

Hillel Adesnik

Article

Sridharan et al Neuron. 2022 Jan 27. pii: S0896-6273(22)

Insert

ChroME-P2A-H2B-mRuby3

Use

AAV

Expression

Mammalian

Available Since

Aug. 2, 2021

Availability

Academic Institutions and Nonprofits only

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eSpCas9-EF-5Nanog_2

Plasmid

#174871

Purpose

CRISPR vector for generating Nanog STREAMING-tag KI cell

Depositor

Hiroshi Ochiai

Article

Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

Insert

sgRNA for mouse Nanog (Nanog Synthetic)

Use

CRISPR

Available Since

Dec. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pNLF1-C_hCOL4A5-Nluc_R1683X

Plasmid

#198124

Purpose

Express hCOL4A5_R1683X in mammalian cells

Depositor

Jeffrey Miner

Article

Omachi et al iScience. 2022 Feb 8;25(3):103891. doi:

Insert

COL4A5 (COL4A5 Human)

Tags

Nluc

Expression

Mammalian

Mutation

R1683X

Promoter

CMV, beta-globin/IgG chimeric intron

Available Since

April 6, 2023

Availability

Academic Institutions and Nonprofits only

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pAL130

Plasmid

#22475

Purpose

Expresses N terminal domain of PIF3 fused to mYFP, for red-light-induced binding to Phy

Depositor

Christopher Voigt

Article

Levskaya et al Nature. 2009 Oct 15;461(7266):997-1001.

Insert

PIF3NT (PIF3 Mouse, Mustard Weed)

Use

Synthetic Biology

Tags

mYFP

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 5, 2009

Availability

Academic Institutions and Nonprofits only

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pDONR221-eGFP_KDEL

Plasmid

#221411

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

eGFP_KDEL

Expression

Mammalian

Available Since

July 18, 2024

Availability

Academic Institutions and Nonprofits only

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PB-Ef1a-RPS27A-UTR-PP7-WPRE

Plasmid

#198339

Purpose

PB plasmid expressing RPS27A with its 3'UTR tagged to PP7 stem loops

Depositor

Michael Ward

Article

De Pace et al Nat Neurosci. 2024 Apr 10. doi: 10.1038/

Insert

RPS27A-3'UTR-PP7 stem loops x24

Use

Piggybac

Tags

PP7 stem loops

Expression

Mammalian

Promoter

Ef1a

Available Since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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hSyn-Aion-ts-citrine-ER

Plasmid

#192575

Purpose

AAV-vector for optogenetic long-term silencing of neurons

Depositor

Simon Wiegert

Article

Rodriguez-Rozada et al Commun Biol. 2022 Jul 9;5(1):687. doi: 1

Insert

Aion

Use

AAV

Tags

ts-Citrine-ER

Expression

Mammalian

Mutation

T59S, E83N, E90Q, E101S, V117R, E123S, C128A,D156…

Promoter

human synapsin

Available Since

Dec. 8, 2022

Availability

Academic Institutions and Nonprofits only

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6K-loop-->R GLUT1-GFP

Plasmid

#200098

Purpose

human GLUT1 with K-->R mutation of 6 lysines in the major cytoplasmic loop and C-terminal GFP tag in pQCXIP vector

Depositor

Jason MacGurn

Article

Qualls-Histed et al iScience. 2023 Feb 6;26(3):106150. doi:

Insert

GLUT1 (SLC2A1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

K-->R mutations of amino acids 225, 229, 230, …

Promoter

CMV

Available Since

May 9, 2023

Availability

Academic Institutions and Nonprofits only

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11K-cyto-->R GLUT1-GFP

Plasmid

#200096

Purpose

human GLUT1 with K-->R mutations for all 11 cytosolic lysines and a C-terminal GFP tag in pQCXIP vector

Depositor

Jason MacGurn

Article

Qualls-Histed et al iScience. 2023 Feb 6;26(3):106150. doi:

Insert

GLUT1 (SLC2A1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

K-->R mutations of amino acids 6, 7, 225, 229,…

Promoter

CMV

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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pNS19-LV-mutRFP-2A-GFP

Plasmid

#113716

Purpose

Lentiviral vector for fluorescent reporter system: expresses a green fluorescent protein that is preceded by an inactive version of a red fluorescent protein (2 ntd substitution at the fluorophore)

Depositor

Gerald Schwank

Article

Savic et al Elife. 2018 May 29;7. pii: 33761. doi: 1

Insert

mutRFP-2A-GFP-IRES-Puro

Use

Lentiviral; Reporter system

Expression

Mammalian

Mutation

Y64L (mutRFP)

Available Since

Aug. 23, 2018

Availability

Academic Institutions and Nonprofits only

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pEasyG1_hph

Plasmid

#184912

Purpose

Template to generate via PCR a single gRNA for expression in S. cerevisiae

Depositor

Jeferson Gross

Article

Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

Insert

gRNA scaffold

Use

CRISPR

Expression

Bacterial and Yeast

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pAG657 H2B-pFAST

Plasmid

#172862

Purpose

Expresses pFAST fused to zebrafish histone H2B in mammalian cells

Depositor

Arnaud Gautier

Article

Benaissa et al Nat Commun. 2021 Nov 30;12(1):6989. doi:

Insert

H2B-pFAST

Tags

Myc tag (C terminal on insert)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pCb.CAG-HaloTag-GA-ZF-KrabA-KIF5B 376-963

Plasmid

#134612

Purpose

Mammalian expression of mouse KIF5B 376-963

Depositor

Marvin Bentley

Article

Yang et al Traffic. 2019 Aug 28. doi: 10.1111/tra.1

Insert

KIF5B (Kif5b Mouse)

Tags

Halo

Expression

Mammalian

Mutation

WT with deletion of the 375 N-terminal residues.

Promoter

Chicken Beta actin

Available Since

April 10, 2020

Availability

Academic Institutions and Nonprofits only

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p.UTA.2.0 miR-27a-3p

Plasmid

#82438

Purpose

Dual Fluorescent endogenous miRNA sensor. Perfect complementary region of hsa-miR-27a-3p

Depositor

Jens Gruber

Article

Lemus-Diaz et al Sci Rep. 2017 Mar 24;7:45197. doi: 10.10

Insert

hsa-miR-27a-3p (MIR27A Human)

Mutation

Three copies hsa-miR-27a-3p Complementary region …

Available Since

March 24, 2017

Availability

Academic Institutions and Nonprofits only

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VPS13C^halo∆1235-1748

Plasmid

#187297

Purpose

Express VPS13C truncation mutant in mammalian cells

Depositor

Pietro De Camilli

Article

Cai et al Proc Natl Acad Sci U S A. 2022 Jul 19;11

Insert

VPS13C truncation mutant (VPS13C Human)

Expression

Mammalian

Available Since

Feb. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pPPC011

Plasmid

#171143

Purpose

Expression of Sp.pCas9-dCas9 and BBa_J23107-MCP-SoxS(R93A/S101A) on pRK2-KmR plasmid

Depositor

Jesse Zalatan

Article

Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(

Insert

Sp.pCas9-dCas9_BBa_J23107-MCP-SoxS(R93A, S101A)

Use

CRISPR

Expression

Bacterial

Mutation

SoxS has R93A and S101A mutations

Promoter

Sp.pCas9, BBa_J23107

Available Since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

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LENTICRISPR-TKT_sgRNA2

Plasmid

#201625

Purpose

CRISPR/Cas9-mediated gene knock-out

Depositor

Alexis Jourdain, Vamsi Mootha

Article

Skinner et al Nat Metab. 2023 May;5(5):765-776. doi: 1

Insert

TKT (TKT Human)

Use

CRISPR and Lentiviral

Available Since

July 26, 2023

Availability

Academic Institutions and Nonprofits only

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