19,660 results

1. pBabe-puro-IMS-RP

   Plasmid

   #24535

   Depositor

   Peter Sorger

   Article

   Albeck et al PLoS Biol. 2008 Dec 2. 6(12):2831-52.

   Insert

   Diablo homolog (DIABLO Human)
   

   Use

   Retroviral

   Tags

   mRFP1

   Expression

   Mammalian

   Mutation

   Deleted residues 56-239

   Available Since

   June 11, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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2. AAV pCAG-FLEX-mScarlet-WPRE

   Plasmid

   #99280

   Purpose

   Can be used to generate AAV virus that will express mScarlet in the presence of Cre

   Depositor

   Rylan Larsen

   Insert

   mScarlet
   

   Use

   AAV and Cre/Lox

   Available Since

   Aug. 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. Rictor_1 shRNA

   Plasmid

   #1853

   Depositor

   David Sabatini

   Article

   Sarbassov et al Science 2005 Feb 18;307(5712):1098-101.

   Insert

   Rictor (RICTOR Human)
   

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Available Since

   July 10, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGL4.22-PGK1-HRE::dLUC

   Plasmid

   #128095

   Purpose

   Hypoxia response element real-time luciferase reporter

   Depositor

   Chi Van Dang

   Article

   Walton et al Cell. 2018 Jun 28;174(1):72-87.e32. doi:

   Insert

   Hypoxia response element (x3) from PGK1 promoter (PGK1 Human)
   

   Use

   Luciferase

   Tags

   drives expression of destabilized luciferase (2CP…

   Expression

   Mammalian

   Promoter

   inserted PGK1 hypoxia response elements (HRE) (x3)

   Available Since

   Sept. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCDH-SFFV-GLuc-CreERT2-mCherry

   Plasmid

   #178185

   Purpose

   the plasmid promotes the constitutive expression of CreERT2 to enable intracellular recombination, Gaussia Luc can be used for in vivo imaging and mCherry is used as a marker for ex vivo analyses.

   Depositor

   Irmela Jeremias

   Article

   Carlet et al Nat Commun. 2021 Sep 27;12(1):5655. doi:

   Inserts

   GLuc
   CreERT2
   mCherry
   

   Use

   Lentiviral

   Available Since

   Jan. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pIS1-Eef25UTR-TOPmut-renilla

   Plasmid

   #38236

   Depositor

   David Sabatini

   Article

   Thoreen et al Nature. 2012 May 2;485(7396):109-13. doi

   Insert

   Eef2_TOPm_5UTR/Renilla (Eef2 Mouse)
   

   Use

   Luciferase

   Expression

   Mammalian

   Mutation

   The first nucleotides of the 5' UTR were cha…

   Promoter

   Endogenous Eef2

   Available Since

   Nov. 7, 2012

   Availability

   Academic Institutions and Nonprofits only

7. N174-MCS

   Plasmid

   #81061

   Purpose

   Lentiviral vector for constitutive gene expression.

   Depositor

   Adam Karpf

   Type

   Empty backbone

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF-1α

   Available Since

   Sept. 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLenti-RIGI-Apex- T2A-EGFP

   Plasmid

   #167288

   Purpose

   Lentiviral expression plasmid encoding an APEX2 RIG-I fusion product in frame with a self-cleaving eGFP. APEX biotin ligase can biotynilate RNAs in proximity of the RLR RIG-I sensor.

   Depositor

   Agnel Sfeir

   Article

   Tigano et al Nature. 2021 Mar;591(7850):477-481. doi:

   Insert

   DDX58 (RIGI Human)
   

   Use

   Lentiviral

   Tags

   APEX2 and EGFP

   Expression

   Mammalian

   Available Since

   April 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. AP2u2-mCherry

   Plasmid

   #27672

   Depositor

   Christien Merrifield

   Article

   Taylor et al PLoS Biol. 2011 Mar . 9(3):e1000604.

   Insert

   Ap2m1 (Ap2m1 Rat)
   

   Tags

   mCherry

   Expression

   Mammalian

   Available Since

   May 4, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDONR223_SUV39H1_WT

    Plasmid

    #82236

    Purpose

    Gateway Donor vector containing SUV39H1 , part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, William Hahn, David Root

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    SUV39H1 (SUV39H1 Human)
    

    Use

    Gateway entry vector

    Mutation

    WT

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. GPD-roGFP

    Plasmid

    #49436

    Purpose

    Expresses thiol redox-sensitive ratiometric sensor roGFP in the mitochondrial intermembrane space of mammalian cells

    Depositor

    Paul Schumacker

    Article

    Waypa et al Circ Res. 2010 Feb 19;106(3):526-35. doi

    Insert

    GPD-roGFP2
    

    Use

    Adenoviral

    Tags

    Glycerol phosphate dehydrogenase, 5' to roGF…

    Expression

    Mammalian

    Mutation

    C48S, Q80R, S147C, and Q204C in Clontech's G…

    Promoter

    CMV

    Available Since

    Dec. 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. p406TDH3

    Plasmid

    #15977

    Depositor

    Nicolas Buchler, Fred Cross

    Insert

    TDH3 promoter + polylinker + CYC1 terminator (TDH3 Budding Yeast)
    

    Expression

    Bacterial and Yeast

    Mutation

    TDH3 promoter is between SacI and XbaI restrictio…

    Available Since

    Nov. 2, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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13. CXCR4-Tango

    Plasmid

    #66262

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    CXCR4 (CXCR4 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. eGFP STAT1 S727E

    Plasmid

    #12305

    Depositor

    Alan Perantoni

    Article

    Timofeeva et al Oncogene. 2006 Dec 7;25(58):7555-64. doi

    Insert

    STAT1 S727E (STAT1 Human)
    

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    S727E

    Available Since

    Sept. 1, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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15. Galphai2-mTurquoise2

    Plasmid

    #69621

    Purpose

    The heterotrimeric G-protein subunit, Gαi isoform 2, tagged with mTurquoise2

    Depositor

    Dorus Gadella

    Article

    van Unen et al PLoS One. 2016 Jan 22;11(1):e0146789. do

    Insert

    Galphai2-mTurquoise2
    

    Tags

    mTurquoise2

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. SOX2se-CPG-TV

    Plasmid

    #70155

    Purpose

    a targeting vector to target the endogenous Sox2 SE CPG island with Snrp-Tomato-polyA

    Depositor

    Rudolf Jaenisch

    Article

    Stelzer et al Cell. 2015 Sep 24;163(1):218-29. doi: 10

    Insert

    5'hSOX2se-Snrp-Tomato-polyA-Pgk-PURO-3'hSOX2se
    

    Use

    Mouse Targeting and Synthetic Biology

    Available Since

    Nov. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCAX APP delta CT

    Plasmid

    #30143

    Depositor

    Dennis Selkoe, Tracy Young-Pearse

    Article

    Young-Pearse et al J Neurosci. 2007 Dec 26. 27(52):14459-69

    Insert

    Amyloid Precursor Protein (APP Human)
    

    Expression

    Mammalian

    Mutation

    deletion of C-terminal intracellular domain (afte…

    Available Since

    July 29, 2011

    Availability

    Academic Institutions and Nonprofits only

18. LeGO-C2

    Plasmid

    #27339

    Depositor

    Boris Fehse

    Article

    Weber et al Mol Ther. 2008 Apr . 16(4):698-706.

    Insert

    SFFV promoter, mCherry
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    April 24, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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19. MED12_pLX307

    Plasmid

    #98350

    Purpose

    Lentiviral expression of MED12

    Depositor

    William Hahn, Sefi Rosenbluh

    Article

    Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

    Insert

    MED12 (MED12 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    R1392Q

    Promoter

    E1Fa

    Available Since

    Aug. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcsII-LMP7-flag

    Plasmid

    #86773

    Purpose

    C-terminal flag-tagged protein expression in mammalian cells, lentiviral vector

    Depositor

    Do-Hyung Kim

    Article

    Yun et al Mol Cell. 2016 Feb 18;61(4):625-39. doi:

    Insert

    immunoproteasome beta subunit 5 (LMP7) (PSMB8 Human)
    

    Use

    Lentiviral

    Tags

    flag

    Expression

    Mammalian

    Promoter

    EF-1a

    Available Since

    Jan. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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