19,660 results

1. pCS2FLAG

   Plasmid

   #16331

   Depositor

   Peter Klein

   Insert

   Flag tag
   

   Use

   Vertebrate expression

   Tags

   Flag

   Mutation

   Flag tag inserted into pCS2+ polylinker

   Available Since

   Dec. 21, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pRK5 HA-Nprl2

   Plasmid

   #99709

   Purpose

   Mammalian expression of HA-Nprl2

   Depositor

   David Sabatini, Kuang Shen

   Article

   Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

   Insert

   Nprl2 (NPRL2 )
   

   Tags

   HA

   Promoter

   CMV

   Available Since

   Nov. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCMVInt

   Plasmid

   #18935

   Depositor

   Michele Calos

   Article

   Groth et al Proc Natl Acad Sci U S A. 2000 May 23. 9

   Insert

   phiC31 integrase (int Streptomyces phage phiC31)
   

   Expression

   Mammalian

   Available Since

   Aug. 13, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pMpGWB337

   Plasmid

   #68061

   Purpose

   Gateway-based vector for Marchantia polymorpha to express a floxed gene, with an expression cassette for Cre-glucocorticoid receptor fusion protein under the control of heat-inducible promoter

   Depositor

   Takayuki Kohchi

   Article

   Nishihama et al Plant Cell Physiol. 2015 Jul 6. pii: pcv

   Type

   Empty backbone

   Use

   Cre/Lox

   Expression

   Plant

   Promoter

   MpEF1alpha promoter

   Available Since

   Sept. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. p3TP-lux

   Plasmid

   #11767

   Depositor

   Joan Massague, Jeff Wrana

   Article

   Wrana et al Cell. 1992 Dec 11. 71(6):1003-14.

   Insert

   3TP
   

   Use

   Luciferase

   Expression

   Mammalian

   Available Since

   Aug. 4, 2006

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

6. pBAD-smURFP-RBS-HO-1

   Plasmid

   #80341

   Purpose

   Expresses smURFP and Heme Oxygenase-1 in bacterial cells.

   Depositor

   Erik Rodriguez, Roger Tsien

   Article

   Rodriguez et al Nat Methods. 2016 Aug 1. doi: 10.1038/nm

   Inserts

   smURFP
   HO-1
   

   Tags

   6 Histidine Tag

   Expression

   Bacterial

   Promoter

   pBAD

   Available Since

   Aug. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pC0078 RESCUE

   Plasmid

   #130661

   Purpose

   pCMV-dCas13b6-mapkNES-GS-dADAR2(E488Q/V351G/S486A/T375S/S370C/P462A/N597I/L332I/I398V/K350I/M383L/D619G/S582T/V440I/S495N/K418E/

   Depositor

   Feng Zhang

   Article

   Abudayyeh et al Science. 2019 Jul 11. pii: science.aax70

   Insert

   dRanCas13b-ADAR2dd (RESCUE)
   

   Expression

   Mammalian

   Available Since

   Oct. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. hWIP1 D314A FLAG

   Plasmid

   #28106

   Depositor

   Lawrence Donehower

   Article

   Moon et al J Biol Chem. 2010 Apr 23. 285(17):12935-

   Insert

   WIP1 (PPM1D Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Contains phosphatase-dead mutation D314A

   Available Since

   April 12, 2011

   Availability

   Academic Institutions and Nonprofits only

9. pDONR221_SLC16A3

   Plasmid

   #131899

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

   Depositor

   RESOLUTE Consortium, Giulio Superti-Furga

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   SLC16A3 (SLC16A3 Human)
   

   Expression

   Mammalian

   Available Since

   Oct. 11, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

10. pLH-sgRNA1

    Plasmid

    #75388

    Purpose

    sgRNA1

    Depositor

    Thoru Pederson

    Article

    Ma et al Nat Biotechnol. 2016 Apr 18. doi: 10.103

    Insert

    sgRNA1
    

    Use

    CRISPR and Lentiviral

    Tags

    no

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    May 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. GATA3-pCDNA3.1

    Plasmid

    #115271

    Purpose

    Expresses human GATA3 in mammalian cells

    Depositor

    Arne S. Schaefer

    Insert

    GATA3 (GATA3 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. VE-cad-eGFP Donor plasmid

    Plasmid

    #92309

    Purpose

    Knock eGFP into human VE-cad locus

    Depositor

    Sean Palecek

    Article

    Bao et al Bioeng Transl Med. 2017 Jun;2(2):191-201

    Insert

    VEcad Homologous Recombination Arms (CDH5 Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    about 30 a.a. truncated at end of protein

    Available Since

    Aug. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. mEGFP-Cdc42-C1

    Plasmid

    #29673

    Depositor

    Ryohei Yasuda

    Article

    Murakoshi et al Nature. 2011 Apr 7;472(7341):100-4. doi:

    Insert

    Cdc42 (CDC42 Human)
    

    Tags

    mEGFP

    Expression

    Mammalian

    Available Since

    June 6, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLKO.1-puro-shNT

    Plasmid

    #109012

    Purpose

    short-hairpin knockdown of targeted gene

    Depositor

    Jacob Corn

    Article

    Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

    Insert

    Non-targeting shRNA
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    hU6 promoter

    Available Since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA3.1 SARS-CoV-2 E

    Plasmid

    #158080

    Purpose

    Expresses SARS-CoV-2 envelope protein in mammalian cells

    Depositor

    Jeremy Luban

    Article

    Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

    Insert

    SARS-CoV-2 envelope (E )
    

    Expression

    Mammalian

    Available Since

    June 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pDEST47-ARL8B-GFP

    Plasmid

    #67404

    Purpose

    Mammalian expression of hArl8b with C-term GFP tag

    Depositor

    Richard Kahn

    Article

    Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

    Insert

    ARL8B (ARL8B Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pInt

    Plasmid

    #18941

    Depositor

    Michele Calos

    Article

    Groth et al Proc Natl Acad Sci U S A. 2000 May 23. 9

    Insert

    phiC31 integrase (int Streptomyces phage phiC31)
    

    Expression

    Bacterial

    Mutation

    under lacZ promoter

    Available Since

    Aug. 13, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pYN4+

    Plasmid

    #78715

    Purpose

    mammalian expression of NHE1

    Depositor

    Larry Fliegel

    Article

    Reddy et al J Biol Chem. 2008 Aug 8;283(32):22018-30

    Insert

    NHE1 (SLC9A1 Human)
    

    Tags

    HA3x

    Expression

    Mammalian

    Mutation

    D720G

    Promoter

    RSV-LTR

    Available Since

    June 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pX330A_D10A-1x2

    Plasmid

    #58772

    Purpose

    Expresses Cas9 nickase and gRNA

    Depositor

    Takashi Yamamoto

    Article

    Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

    Insert

    humanized S. pyogenes Cas9 (D10A) nickase
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    D10A nickase-converting mutation

    Promoter

    CBh

    Available Since

    Nov. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGal4AD-CIB1

    Plasmid

    #28245

    Purpose

    Expresses Gal4 Activation Domain fused to CIB1 for blue-light-induced transcription activation

    Depositor

    Chandra Tucker

    Article

    Kennedy et al Nat Methods. 2010 Dec;7(12):973-5. doi:

    Insert

    Gal4AD (CIB1, GAL4 Budding Yeast)
    

    Tags

    CIB1

    Expression

    Yeast

    Promoter

    ADH1

    Available Since

    March 18, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge