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pGL3B-1081

Plasmid

#18759

Depositor

Clark Distelhorst

Article

Wang et al Oncogene. 2006 Mar 23. 25(13):1903-13.

Insert

thioredoxin-interacting protein promoter (Txnip Mouse)

Use

Luciferase

Available Since

Aug. 4, 2008

Availability

Academic Institutions and Nonprofits only
mRFP-wGBD

Plasmid

#26733

Depositor

William Bement

Article

Benink et al J Cell Biol. 2005 Jan 31. 168(3):429-39.

Insert

Neuronal Wiskott-Aldrich Syndrome protein (WASL Human)

Use

Reporter for cdc42

Tags

mRFP

Expression

Mammalian

Mutation

Cdc42 binding domain of N-WASP

Available Since

April 21, 2011

Availability

Academic Institutions and Nonprofits only
pDONR223 SARS-CoV-2 NSP9

Plasmid

#141263

Purpose

Gateway-compatible Entry vector, with insert of NSP9 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

Depositor

Fritz Roth

Article

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

NSP9 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)

Use

Gateway-compatible entry vector

Mutation

Many synonymous changes due to codon optimization

Available Since

April 1, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pCDNA3.1-5'dGFP3'

Plasmid

#26425

Depositor

Erin Schuman

Article

Sutton et al Science. 2004 Jun 25. 304(5679):1979-83.

Insert

5'dGFP3'

Tags

CamKIIa 3'-UTR, CamKIIa 5'-UTR, and dGF…

Expression

Mammalian

Available Since

Oct. 18, 2010

Availability

Academic Institutions and Nonprofits only
pBABE-hLin28B

Plasmid

#26358

Depositor

George Daley

Article

Viswanathan et al Nat Genet. 2009 Jul . 41(7):843-8.

Insert

lin-28 homolog B (LIN28B Human)

Use

Retroviral

Expression

Mammalian

Mutation

None

Available Since

Nov. 3, 2010

Availability

Academic Institutions and Nonprofits only

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pDONR223 SARS-CoV-2 ORF7B

Plasmid

#141277

Purpose

Gateway-compatible Entry vector, with insert of ORF7B gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

Depositor

Fritz Roth

Article

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

ORF7B (ORF7b SARS-CoV-2 isolate Wuhan-Hu-1)

Use

Gateway-compatible entry vector

Mutation

Many synonymous changes due to codon optimization

Available Since

April 1, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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FUW-SOK

Plasmid

#20327

Depositor

Rudolf Jaenisch

Article

Carey et al Proc Natl Acad Sci U S A. 2009 Jan 6. 10

Insert

Sox2-F2A-Oct4-T2A-Klf4 (Sox2, Pou5f1, Klf4 Mouse)

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 23, 2009

Availability

Academic Institutions and Nonprofits only

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pIRESneo-FLAG/HA EYFP

Plasmid

#10825

Depositor

Thomas Tuschl

Article

Meister et al Mol Cell. 2004 Jul 23. 15(2):185-97.

Insert

EYFP

Tags

FLAG and HA

Expression

Mammalian

Available Since

Nov. 14, 2005

Availability

Academic Institutions and Nonprofits only

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MMTV-SV40-Bssk

Plasmid

#1824

Depositor

Philip Leder

Article

Ornitz et al Proc Natl Acad Sci U S A 1991 Feb 1;88(3

Type

Empty backbone

Expression

Mammalian

Available Since

July 18, 2006

Availability

Academic Institutions and Nonprofits only

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Myc-Foxo1 AAA (pCMV5)

Plasmid

#17547

Depositor

Domenico Accili

Insert

Foxo1 AAA (Foxo1 Mouse)

Tags

Myc

Expression

Mammalian

Mutation

Changed T24A T253A S316A

Promoter

CMV

Available Since

Oct. 20, 2008

Availability

Academic Institutions and Nonprofits only
pICE

Plasmid

#46960

Purpose

Plasmid for cloning and constitutive or doxycycline-inducible expression of cDNAs. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.

Depositor

Steve Jackson

Article

Britton et al J Cell Biol. 2013 Jul 29.

Type

Empty backbone

Expression

Mammalian

Available Since

Aug. 20, 2013

Availability

Academic Institutions and Nonprofits only

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pLenti-PGK-KRAS4A(G12V)

Plasmid

#35634

Depositor

Daniel Haber

Article

Singh et al Cell. 2012 Feb 17;148(4):639-50.

Insert

K-Ras4A(G12V) (KRAS Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Glycine 12 to Valine

Promoter

PGK

Available Since

April 18, 2012

Availability

Academic Institutions and Nonprofits only
pIREShyg-PA-GFP-alpha Tubulin

Plasmid

#12296

Depositor

Patricia Wadsworth

Article

Tulu et al Curr Biol. 2003 Oct 28. 13(21):1894-9.

Insert

alpha Tubulin (TUBA1B Human)

Tags

PA-GFP

Expression

Mammalian

Available Since

Aug. 11, 2006

Availability

Academic Institutions and Nonprofits only

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pECE-HA-AMPKbeta1 wt

Plasmid

#31666

Depositor

Anne Brunet

Article

Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

Insert

protein kinase, AMP-activated, beta 1 non-catalytic subunit (PRKAB1 Human)

Tags

HA (YDVPDYASL)

Expression

Mammalian

Available Since

April 11, 2012

Availability

Academic Institutions and Nonprofits only

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p2xFlag CMV2-YAP2-1st&2nd WW mutant

Plasmid

#19048

Depositor

Marius Sudol

Article

Oka et al J Biol Chem. 2008 Oct 10;283(41):27534-2

Insert

Yes-kinase associated protein (YAP1 Human)

Tags

2xFlag

Expression

Mammalian

Mutation

Tryptophan 199 changed to Alanine,and Proline 202…

Available Since

Sept. 8, 2008

Availability

Academic Institutions and Nonprofits only

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pCS-H2B-cerulean

Plasmid

#53748

Purpose

Contains a fusion of human histone H2B to cerulean fluorescent protein in the pCS2+ vector.

Depositor

Sean Megason

Article

Megason et al Methods Mol Biol. 2009;546:317-32. doi:

Insert

histone H2B (H2BC21 Human)

Tags

Cerulean

Expression

Mammalian

Promoter

simian CMV IE94

Available Since

July 8, 2014

Availability

Academic Institutions and Nonprofits only

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GFP-Rab5DN(S34N)

Plasmid

#35141

Depositor

Sergio Grinstein

Article

Bohdanowicz et al Mol Biol Cell. 2012 Jan;23(1):176-87. Ep

Insert

Rab5a (RAB5A Human)

Tags

EGFP

Expression

Mammalian

Mutation

Dominant Negative- S34N

Promoter

CMV

Available Since

March 20, 2012

Availability

Academic Institutions and Nonprofits only
CRISPseq-BFP-backbone

Plasmid

#85707

Purpose

Backbone for CRISPR/Cas9 screening with single cell RNA-seq. Lentiviral plasmid for cloning of gRNAs and Unique Guide Index (UGI), with a BFP fluorescent marker. Does NOT include Cas9.

Depositor

Ido Amit

Article

Jaitin et al Cell. 2016 Dec 15;167(7):1883-1896.e15.

Type

Empty backbone

Use

Lentiviral

Available Since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pTK-Twist

Plasmid

#36977

Depositor

Bob Weinberg

Article

Guo et al Cell. 2012 Mar 2;148(5):1015-28.

Insert

Twist (Twist1 )

Use

Lentiviral

Available Since

June 6, 2012

Availability

Academic Institutions and Nonprofits only
pEGFP-C2 SENP2

Plasmid

#13382

Depositor

Mary Dasso

Article

Hang et al J Biol Chem. 2002 May 31. 277(22):19961-

Insert

SENP2 (SENP2 Human)

Tags

EGFP

Expression

Mammalian

Available Since

Nov. 16, 2006

Availability

Academic Institutions and Nonprofits only

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