We narrowed to 31,565 results for: REP

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DT12A

Plasmid

#80416

Purpose

Expresses 12 CUG repeats in the context of human DMPK genomic segment containing exons 11-15 with repeats inserted at site in exon 15 that contains the repeats

Depositor

Thomas Cooper

Article

Ho et al EMBO J. 2004 Aug 4;23(15):3103-12. Epub

Insert

human DMPK exons 11-15 with 12 CTG repeats (DMPK Human)

Expression

Mammalian

Available Since

Sept. 6, 2016

Availability

Academic Institutions and Nonprofits only

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Sg-A

Plasmid

#83807

Purpose

Express sgRNA targeting SA site

Depositor

Bo Feng

Article

He et al Nucleic Acids Res. 2016 May 19;44(9):e85

Insert

SgRNA

Use

CRISPR

Available Since

Nov. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pCRU5HT1-inLuc

Plasmid

#58954

Purpose

HTLV-1 transfer vector that encodes firefly luciferase gene interrupted by a gamma-globin intron. The reporter gene is transduced only after the completion of viral cycle replication.

Depositor

David Derse, Dmitriy Mazurov

Article

Mazurov et al PLoS Pathog. 2010 Feb 26;6(2):e1000788.

Insert

inLuc

Use

Retroviral

Promoter

CMV/R/U5

Available Since

Sept. 16, 2014

Availability

Academic Institutions and Nonprofits only

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pCXLE-dCas9VP192-T2A-EGFP-shP53

Plasmid

#69535

Purpose

Episomal plasmid encoding dCas9VP192 and p53 shRNA

Depositor

Timo Otonkoski

Article

Balboa et al Stem Cell Reports. 2015 Sep 8;5(3):448-5

Insert

dCas9-dCas9VP192-GFP-shp53

Use

CRISPR

Tags

EGFP

Promoter

CAG

Available Since

Nov. 20, 2015

Availability

Academic Institutions and Nonprofits only

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sgRNA 1 GAPDH

Plasmid

#83808

Purpose

Express Sg-1 sgRNA targeting GAPDH

Depositor

Bo Feng

Article

He et al Nucleic Acids Res. 2016 May 19;44(9):e85

Insert

SgRNA

Use

CRISPR

Available Since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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Sth1Cas9n cytosine base editor

Plasmid

#194209

Purpose

Sth1Cas9-D9A-nickase_rAPOBEC1_cytosine base editor for RNA recording

Depositor

Chase Beisel

Article

Jiao et al Nat Biotechnol. 2023 Jan 5. doi: 10.1038

Insert

Sth1Cas9-D9A nickase

Use

CRISPR

Expression

Bacterial

Mutation

Changed Aspartate 9 to Alanine.

Available Since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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pLPO2

Plasmid

#209976

Purpose

Contains Level 0 Part: replication origin (SH71rep) for the construction of Level 1 plasmids

Depositor

Thorsten Mascher

Article

Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

Insert

replication origin (SH71_rep)

Expression

Bacterial

Available Since

Sept. 13, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pCVL.SFFV.Kozak.HA.NLS.SceOpt.IRES.BFP

Plasmid

#45574

Purpose

Expresses I-Sce I nuclease in mammalian cells with BFP tracker in lentiviral backbone

Depositor

Andrew Scharenberg

Article

Kuhar et al Nucleic Acids Res. 2013 Oct 10.

Insert

co I-Sce I

Use

Lentiviral

Tags

HA, IRES BFP, and NLS

Expression

Mammalian

Mutation

co indicates codon optimization for mammalian exp…

Promoter

SFFV

Available Since

Nov. 4, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3-NP-B/BRISBANE/60/2008

Plasmid

#187921

Purpose

Expresses nucleoprotein of Influenza B/Brisbane/60/2008 virus in mammalian cells

Depositor

Arindam Mondal

Article

Kedia et al Front Microbiol. 2022 May 25;13:868367.

Insert

Influenza B Nucleoprotein

Tags

UNTAGGED

Expression

Mammalian

Promoter

CMV Promoter

Available Since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pLPO1

Plasmid

#209975

Purpose

Contains Level 0 Part: replication origin (256rep) for the construction of Level 1 plasmids

Depositor

Thorsten Mascher

Article

Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

Insert

replication origin (256_rep)

Expression

Bacterial

Available Since

Sept. 13, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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3xDBE_RE:NLuc

Plasmid

#178318

Purpose

FOXO NLuc luciferase reporter

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Methods Mol Biol. 2022;2524:409-432. doi

Insert

FOXO NLuc Reporter

Use

Luciferase and Synthetic Biology

Expression

Mammalian

Available Since

July 20, 2022

Availability

Academic Institutions and Nonprofits only

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4xHRE_RE:RedF

Plasmid

#178314

Purpose

HRE RedFirefly luciferase reporter

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Methods Mol Biol. 2022;2524:409-432. doi

Insert

HRE RedFirefly reporter

Use

Luciferase and Synthetic Biology

Expression

Mammalian

Available Since

July 20, 2022

Availability

Academic Institutions and Nonprofits only

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pTK_BsmBI_LacZ_Cerulean_Nanotag_60

Plasmid

#130528

Purpose

Enhancer cloning nanotag reporter vector

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

Insert

LacZ

Use

Enhancer cloning nanotag reporter vector

Promoter

thymidine kinase minimal promoter

Available Since

Nov. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pEC2861 (pSpy1C-PgyrA_ffluc)

Plasmid

#218511

Purpose

replicative E. coli-S. pyogenes shuttle plasmid, constitutive expression of firefly luciferase reporter

Depositor

Emmanuelle Charpentier

Article

Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

Insert

ffluc

Use

Luciferase

Mutation

deletion of Plac_mrfp cassette

Promoter

PgyrA

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pTK_Citrine_Nanotag_24_positive_Control

Plasmid

#130570

Purpose

Enhancer cloning nanotag reporter vector

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

Insert

FoxD3_cranial_neural_crest_enhancer_NC1

Use

Enhancer cloning nanotag reporter vector

Promoter

thymidine kinase minimal promoter

Available Since

Sept. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-NP-V5-B/BRISBANE/60/2008

Plasmid

#187923

Purpose

Expresses C terminal V5 tagged nucleoprotein of Influenza B/Brisbane/60/2008 virus in mammalian cells

Depositor

Arindam Mondal

Article

Kedia et al Front Microbiol. 2022 May 25;13:868367.

Insert

Influenza B Nucleoprotein

Tags

V5

Expression

Mammalian

Promoter

CMV Promoter

Available Since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pTK_BsmBI_LacZ_Citrine_Nanotag_45

Plasmid

#130525

Purpose

Enhancer cloning nanotag reporter vector

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

Insert

LacZ

Use

Enhancer cloning nanotag reporter vector

Promoter

thymidine kinase minimal promoter

Available Since

Oct. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pTK_BsmBI_LacZ_Cherry_Nanotag_106

Plasmid

#130547

Purpose

Enhancer cloning nanotag reporter vector

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

Insert

LacZ

Use

Enhancer cloning nanotag reporter vector

Promoter

thymidine kinase minimal promoter

Available Since

Sept. 25, 2019

Availability

Academic Institutions and Nonprofits only

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sgRNA 2 GAPDH

Plasmid

#83809

Purpose

Express Sg-2 sgRNA targeting GAPDH

Depositor

Bo Feng

Article

He et al Nucleic Acids Res. 2016 May 19;44(9):e85

Insert

SgRNA

Use

CRISPR

Available Since

Dec. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pET-28c-F30-2xdBroccoli-47CAG

Plasmid

#237217

Purpose

Express F30-2xdBroccoli tagged 47 repeats of CAG RNA in bacteria.

Depositor

Mingxu You

Article

Xue et al Nucleic Acids Res. 2023 Sep 8;51(16):833

Insert

47 CAG trinucleotide repeats RNA

Expression

Bacterial

Available Since

June 2, 2025

Availability

Academic Institutions and Nonprofits only

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