We narrowed to 5,443 results for: 129

1. co frp pcDNA3.1(+)

   Plasmid

   #129090

   Purpose

   Expression of frp in mammalian cells

   Depositor

   Stefan Hell

   Article

   Gregor et al Proc Natl Acad Sci U S A. 2019 Dec 2. pi

   Insert

   co frp
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCAG-Positron

   Plasmid

   #129253

   Purpose

   Express Positron in mammalian cells

   Depositor

   Eric Schreiter

   Article

   Abdelfattah et al Nat Commun. 2020 Jul 10;11(1):3444. doi:

   Insert

   Positron
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   D92N, E199V

   Promoter

   CAG

   Available since

   Aug. 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. co luxA pcDNA3.1(+)

   Plasmid

   #129085

   Purpose

   Expression of luxA in mammalian cells

   Depositor

   Stefan Hell

   Article

   Gregor et al Proc Natl Acad Sci U S A. 2019 Dec 2. pi

   Insert

   co luxA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. co luxB pcDNA3.1(+)

   Plasmid

   #129086

   Purpose

   Expression of luxB in mammalian cells

   Depositor

   Stefan Hell

   Article

   Gregor et al Proc Natl Acad Sci U S A. 2019 Dec 2. pi

   Insert

   co luxB
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCas9/VRQR-sgRNA (BbsI)

   Plasmid

   #129725

   Purpose

   Expressing SpCas9/VRQR mutant and sgRNA for target sequence with NGA PAM

   Depositor

   Elina Ikonen

   Article

   Li et al Nat Methods. 2019 Aug 26. pii: 10.1038/s

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. mCherry-TrioN

   Plasmid

   #129621

   Purpose

   RhoGEF

   Depositor

   Dorus Gadella

   Article

   Reinhard et al Small GTPases. 2019 Aug 30:1-15. doi: 10

   Insert

   N-terminal part of TRIO
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pHis APEX2-eIF4A1

   Plasmid

   #129645

   Purpose

   Express APEX2 fusion to DEAD-box translation factor eIF4A1 in bacteria

   Depositor

   Nicholas Ingolia

   Article

   Padron et al Mol Cell. 2019 Aug 22;75(4):875-887.e5.

   Insert

   eukaryotic translation initiation factor 4A1 (EIF4A1 Human)
   

   Use

   Tags

   6xHis and APEX2

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pGEX-4T-1-3xFLAG-HSP27

   Plasmid

   #129229

   Purpose

   Encodes FLAG tagged human HSP27 for purification by GST affinity

   Depositor

   Kevin Janes

   Article

   Smolko et al Sci Rep. 2019 Dec 19;9(1):19409. doi: 10

   Insert

   HSP27 (HSPB1 Human)
   

   Use

   Tags

   FLAG and GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pGEX-2T-Rac1-wt

   Plasmid

   #12977

   Depositor

   Gary Bokoch

   Insert

   Rac1 (RAC1 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 27, 2006

   Availability

   Academic Institutions and Nonprofits only

10. HR180-LGR5-iCT

    Plasmid

    #129094

    Purpose

    KI iCaspase9-tdTomato with a puromycin/mRuby selection cassette into human LGR5 locus

    Depositor

    Toshiro Sato

    Article

    Shimokawa et al Nature. 2017 May 11;545(7653):187-192. d

    Insert

    iCaspase9-T2A-tdTomato and LGR5 homology arms (LGR5 Human)
    

    Use

    Homologous recombination donor plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pRK5-myc-RhoA-Q63L

    Plasmid

    #12964

    Depositor

    Gary Bokoch

    Insert

    RhoA constitutively active (RHOA Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Q63L Constitutively active

    Promoter

    Available since

    Oct. 27, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pRK5-myc-RhoA-T19N

    Plasmid

    #12963

    Depositor

    Gary Bokoch

    Insert

    RhoA DN (RHOA Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    T19N Dominant negative

    Promoter

    Available since

    March 2, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pSH-EFIRES-P-Seipin-miniIAA7-mScarlet-I

    Plasmid

    #129721

    Purpose

    AAVS1 safe harbor targeting vector expressing miniIAA7-mScarlet-I tagged Seipin

    Depositor

    Elina Ikonen

    Article

    Li et al Nat Methods. 2019 Aug 26. pii: 10.1038/s

    Insert

    Seipin (BSCL2 Human)
    

    Use

    CRISPR and TALEN; Human safe harbor locus (a avs1…

    Tags

    miniIAA7-mScarlet-I

    Expression

    Mammalian

    Mutation

    silent mutations

    Promoter

    EF1a

    Available since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3.1+/-FLI1-C‐(K)‐DYK

    Plasmid

    #129497

    Purpose

    Mammalian expression of human FLI1

    Depositor

    Marjorie Brand

    Article

    Palii et al Cell Stem Cell. 2019 May 2;24(5):812-820

    Insert

    FLI1 (FLI1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pSH-EFIRES-P-AtAFB2-mCherry

    Plasmid

    #129716

    Purpose

    AAVS1 safe harbor targeting vector expressing AtAFB2-mCherry

    Depositor

    Elina Ikonen

    Article

    Li et al Nat Methods. 2019 Aug 26. pii: 10.1038/s

    Insert

    AFB2 (AFB2 Mustard Weed)
    

    Use

    CRISPR and TALEN; Human safe harbor locus (a avs1…

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    codon optimized

    Promoter

    EF1a

    Available since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pGEX-2T-Rac1-Q61L

    Plasmid

    #12979

    Depositor

    Gary Bokoch

    Insert

    Rac1 constitutively active (RAC1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Q61L Constitutively active

    Promoter

    Available since

    Oct. 27, 2006

    Availability

    Academic Institutions and Nonprofits only

17. pcDNA3-EGFP-Rac1-wt

    Plasmid

    #12980

    Depositor

    Gary Bokoch

    Article

    Subauste et al J Biol Chem. 2000 Mar 31. 275(13):9725-3

    Insert

    Rac1 (RAC1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 27, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3 AIF-mScarlet

    Plasmid

    #129576

    Purpose

    Expression of mScarlet fused AIF

    Depositor

    Stephen Tait

    Article

    Riley et al EMBO J. 2018 Sep 3;37(17). pii: embj.201

    Insert

    AIF (AIFM1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    None

    Promoter

    Available since

    Aug. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. K560GFP

    Plasmid

    #129761

    Purpose

    Expresses Drosophila Kinesin-1 truncated after coil 1 at 560 and tagged with GFP for single-molecule motor assays

    Depositor

    William Hancock

    Insert

    KHC (Khc Fly)
    

    Use

    Tags

    GFP and His6

    Expression

    Bacterial

    Mutation

    Truncation at 559

    Promoter

    Available since

    Nov. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. UbC-(mNeonGreen)4-tDeg

    Plasmid

    #129404

    Purpose

    (mNeonGreen)4-tDeg fluorogenic protein

    Depositor

    Samie Jaffrey

    Article

    Wu et al Nat Methods. 2019 Sep;16(9):862-865. doi

    Insert

    (mNeonGreen)4-tDeg
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    UbC

    Available since

    Aug. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pET28aAviHisCD72cCTLD

    Plasmid

    #129066

    Purpose

    generation of biotinylated mouse CD72c CTLD protein using bacteria

    Depositor

    Takeshi Tsubata

    Article

    Akatsu et al J Exp Med. 2016 Nov 14;213(12):2691-2706

    Insert

    CD72c (Cd72 Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pB-Gaolf-Integration

    Plasmid

    #129456

    Purpose

    piggyBac transposon vector that Inducibly (Tet-On) expresses Gaolf

    Depositor

    Sriram Kosuri

    Article

    Jones et al Cell Syst. 2019 Mar 27;8(3):254-260.e6.

    Insert

    G alpha olf (Gnal Mouse)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE (Tet-On)

    Available since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pGEX-4T-1-3xHA

    Plasmid

    #129567

    Purpose

    Empty backbone for HA tag

    Depositor

    Kevin Janes

    Article

    Smolko et al Sci Rep. 2019 Dec 19;9(1):19409. doi: 10

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. JME4472

    Plasmid

    #129658

    Purpose

    URA3ex_CrisprCas9-yl_RFP: CAS9 vector with URA3ex marker for gRNA cloning using GoldenGate

    Depositor

    Tristan Rossignol

    Article

    Larroude et al Biotechnol Lett. 2020 Jan 23. pii: 10.10

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pSH-EFIRES-P-AtAFB2

    Plasmid

    #129715

    Purpose

    AAVS1 safe harbor targeting vector expressing AtAFB2

    Depositor

    Elina Ikonen

    Article

    Li et al Nat Methods. 2019 Aug 26. pii: 10.1038/s

    Insert

    AFB2 (AFB2 Mustard Weed)
    

    Use

    CRISPR and TALEN; Human safe harbor locus (a avs1…

    Tags

    no

    Expression

    Mammalian

    Mutation

    codon optimized

    Promoter

    EF1a

    Available since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pRK5-myc-Cdc42-Q61L

    Plasmid

    #12974

    Depositor

    Gary Bokoch

    Insert

    Cdc42 constitutively active (CDC42 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Q61L Constitutively active

    Promoter

    Available since

    March 2, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pEGFP-mAV

    Plasmid

    #129472

    Purpose

    Mammalian expression of full-length mouse archvillin (Supervillin, isoform 2)

    Depositor

    Elizabeth Luna

    Article

    Oh et al J Cell Sci. 2003 Jun 1. 116(Pt 11):2261-

    Insert

    Supervillin, isoform 2 (Svil Mouse)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pTfR-mNeonGreen

    Plasmid

    #129608

    Purpose

    In vivo visualization of Transferrin receptors (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Chertkova et al bioRxiv 160374

    Insert

    Transferrin
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pSpdCas9-huTET1CD-T2A-mCherry(PX458)

    Plasmid

    #129027

    Purpose

    targeted DNA demethylation, expression of dCas9-huTET1CD-T2A-mCherry and cloning backbone for sgRNA

    Depositor

    Julia K Polansky

    Article

    Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

    Insert

    dCas9-huTET1CD, SgRNA cloning site
    

    Use

    Tags

    HA-Tag, NLS and T2A-mCherry

    Expression

    Mammalian

    Mutation

    dCas9 (D10A;H840A)

    Promoter

    CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)

    Available since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pEGFP-N1-mCherry-ApoB-TagBFP

    Plasmid

    #129383

    Purpose

    Plasmid bearing the editing target of APOBEC1 fused between the mCherry and the Tag-BFP one. In presence of APOBEC1, the CAA codon in ApoB is edited to a Stop codon (UAA), leading to loss of Tag-BFP

    Depositor

    Silvestro Conticello

    Article

    Chieca et al Methods Mol Biol. 2021;2181:69-81. doi:

    Insert

    mCherry-ApoB-Tag-BFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available since

    May 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge