Skip to main content
Addgene
Showing: 121 - 160 of 234 results

  1. pVH666

    Plasmid
    #169610
    Purpose
    Tier-2 vector encoding PTtgO2-driven SEAP-p2A-iRFP670, PmPGK1-driven TtgA and PmPGK1-driven mRuby2 expression (PTtgO2-CMVmin-2-SEAP-p2A-iRFP670-pA::PmPGK1-TtgA-pA::PmPGK1-mRuby2-pA).
    Depositor
    Martin Fussenegger
    Article
    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10
    Insert
    tetracycline-controlled SEAP and iRFP expression, PPGK-driven TtgA expression cassette and PPGK-driven YPet expression cassette
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    tetO7-PCMVmin / PPGK / PPGK
    Available since
    July 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    169610-plasmid-map-sequence-id-366627

    Enlarge

  2. pVH664

    Plasmid
    #169608
    Purpose
    Tier-2 vector encoding PTetO7-driven SEAP-p2A-iRFP670, PmPGK1-driven rtTA and PmPGK1-driven YPet expression (PTetO7-CMVmin-2-SEAP-p2A-iRFP670-pA::PmPGK1-rtTA-pA::PmPGK1-YPet-pA).
    Depositor
    Martin Fussenegger
    Article
    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10
    Insert
    tetracycline-controlled SEAP and iRFP expression, PPGK-driven rtTA expression cassette and PPGK-driven YPet expression cassette
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    tetO7-PCMVmin / PPGK / PPGK
    Available since
    July 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    169608-plasmid-map-sequence-id-366623

    Enlarge

  3. pVH663

    Plasmid
    #169607
    Purpose
    Tier-2 vector encoding PTetO7-driven SEAP-p2A-iRFP670, PmPGK1-driven tTA and PmPGK1-driven YPet expression (PTetO7-CMVmin-2-SEAP-p2A-iRFP670-pA::PmPGK1-tTA-pA::PmPGK1-YPet-pA).
    Depositor
    Martin Fussenegger
    Article
    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10
    Insert
    tetracycline-controlled SEAP and iRFP expression, PPGK-driven tTA expression cassette and PPGK-driven YPet expression cassette
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    tetO7-PCMVmin / PPGK / PPGK
    Available since
    June 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    169607-plasmid-map-sequence-id-365601

    Enlarge

  4. pWZ699

    Plasmid
    #208117
    Purpose
    Payload yeast assembly vector with TK-mActb on backbone
    Depositor
    Jef Boeke
    Article
    Zhang et al Nature. 2023 Nov 1. doi: 10.1038/s41586-
    Type
    Empty backbone
    Use
    Synthetic Biology; Copy number inducible
    Tags
    Expression
    Bacterial, Mammalian, and Y…
    Mutation
    Promoter
    Human PGK1 for thymidine kinase
    Available since
    Nov. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    208117-plasmid-map-sequence-id-409744

    Enlarge

  6. pPL001-BBTK

    Plasmid
    #184751
    Purpose
    Big-IN positive control payload encoding EF1a-driven GFP-T2A-BSD. Harbors a backbone counterselectable TK cassette.
    Depositor
    Jef Boeke
    Article
    Brosh et al Proc Natl Acad Sci U S A. 2021 Mar 9;118
    Insert
    pEF1a-eGFP-T2A-BSD-bGHpA
    Use
    Cre/Lox, Mouse Targeting, and Synthetic Biology ;…
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Jan. 17, 2023
    Availability
    Academic Institutions and Nonprofits only
    184751-plasmid-map-sequence-id-365380

    Enlarge

  13. pVG143

    Plasmid
    #227754
    Purpose
    Expresses a version of light-inducible transcription factor EL222 (Leu87Ile) under a strong constitutive PGK1pr
    Depositor
    Sahand Jamal Rahi
    Article
    Gligorovski et al bioRxiv 2024.02.28.582517
    Insert
    EL222 codon-optimized for budding yeast
    Use
    Tags
    Expression
    Yeast
    Mutation
    Leu87Ile
    Promoter
    PGK1pr
    Available since
    Jan. 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    227754-plasmid-map-sequence-id-458037

    Enlarge

  14. pVG134

    Plasmid
    #227770
    Purpose
    Expresses a version of light-inducible transcription factor EL222 (Glu84Gly) under a strong constitutive PGK1pr
    Depositor
    Sahand Jamal Rahi
    Article
    Gligorovski et al bioRxiv 2024.02.28.582517
    Insert
    EL222 codon-optimized for budding yeast
    Use
    Tags
    Expression
    Yeast
    Mutation
    Glu84Gly mutant
    Promoter
    PGK1pr
    Available since
    Jan. 15, 2025
    Availability
    Academic Institutions and Nonprofits only
    227770-plasmid-map-sequence-id-458112

    Enlarge

  15. pVG178

    Plasmid
    #227761
    Purpose
    Expresses a version of light-inducible transcription factor EL222 (Glu84Lys) under a strong constitutive PGK1pr
    Depositor
    Sahand Jamal Rahi
    Article
    Gligorovski et al bioRxiv 2024.02.28.582517
    Insert
    EL222 codon-optimized for budding yeast
    Use
    Tags
    Expression
    Yeast
    Mutation
    Glu84KLys mutant
    Promoter
    PGK1pr
    Available since
    Jan. 15, 2025
    Availability
    Academic Institutions and Nonprofits only
    227761-plasmid-map-sequence-id-458111

    Enlarge

  16. pVG144

    Plasmid
    #227760
    Purpose
    Expresses a version of light-inducible transcription factor EL222 (Val121Met) under a strong constitutive PGK1pr
    Depositor
    Sahand Jamal Rahi
    Article
    Gligorovski et al bioRxiv 2024.02.28.582517
    Insert
    EL222 codon-optimized for budding yeast
    Use
    Tags
    Expression
    Yeast
    Mutation
    Val121Met mutant
    Promoter
    PGK1pr
    Available since
    Jan. 15, 2025
    Availability
    Academic Institutions and Nonprofits only
    227760-plasmid-map-sequence-id-458109

    Enlarge

  17. pVG150

    Plasmid
    #227759
    Purpose
    Expresses a version of light-inducible transcription factor EL222 (Ser137Asn) under a strong constitutive PGK1pr
    Depositor
    Sahand Jamal Rahi
    Article
    Gligorovski et al bioRxiv 2024.02.28.582517
    Insert
    EL222 codon-optimized for budding yeast
    Use
    Tags
    Expression
    Yeast
    Mutation
    Ser137Asn mutant
    Promoter
    PGK1pr
    Available since
    Jan. 15, 2025
    Availability
    Academic Institutions and Nonprofits only
    227759-plasmid-map-sequence-id-458108

    Enlarge

  18. pVG124

    Plasmid
    #227758
    Purpose
    Expresses a version of light-inducible transcription factor EL222 (Thr83Ser) under a strong constitutive PGK1pr
    Depositor
    Sahand Jamal Rahi
    Article
    Gligorovski et al bioRxiv 2024.02.28.582517
    Insert
    EL222 codon-optimized for budding yeast
    Use
    Tags
    Expression
    Yeast
    Mutation
    Thr83Ser mutant
    Promoter
    PGK1pr
    Available since
    Jan. 15, 2025
    Availability
    Academic Institutions and Nonprofits only
    227758-plasmid-map-sequence-id-458107

    Enlarge

  19. pVG121

    Plasmid
    #227757
    Purpose
    Expresses a version of light-inducible transcription factor EL222 (Gly82Val) under a strong constitutive PGK1pr
    Depositor
    Sahand Jamal Rahi
    Article
    Gligorovski et al bioRxiv 2024.02.28.582517
    Insert
    EL222 codon-optimized for budding yeast
    Use
    Tags
    Expression
    Yeast
    Mutation
    Gly80Glu mutant
    Promoter
    PGK1pr
    Available since
    Jan. 15, 2025
    Availability
    Academic Institutions and Nonprofits only
    227757-plasmid-map-sequence-id-458105

    Enlarge

  20. pVG123

    Plasmid
    #227755
    Purpose
    Expresses a version of light-inducible transcription factor EL222 (Ala157Val) under a strong constitutive PGK1pr
    Depositor
    Sahand Jamal Rahi
    Article
    Gligorovski et al bioRxiv 2024.02.28.582517
    Insert
    EL222 codon-optimized for budding yeast
    Use
    Tags
    Expression
    Yeast
    Mutation
    Ala157Val mutant
    Promoter
    PGK1pr
    Available since
    Jan. 15, 2025
    Availability
    Academic Institutions and Nonprofits only
    227755-plasmid-map-sequence-id-458101

    Enlarge

  21. pVG147

    Plasmid
    #227753
    Purpose
    Expresses a version of light-inducible transcription factor EL222 (Gly94Cys) under a strong constitutive PGK1pr
    Depositor
    Sahand Jamal Rahi
    Article
    Gligorovski et al bioRxiv 2024.02.28.582517
    Insert
    EL222 codon-optimized for budding yeast
    Use
    Tags
    Expression
    Yeast
    Mutation
    Gly94Cys mutant
    Promoter
    PGK1pr
    Available since
    Jan. 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    227753-plasmid-map-sequence-id-458034

    Enlarge

  22. pVG120

    Plasmid
    #227752
    Purpose
    Expresses a version of light-inducible transcription factor EL222 (Asp89Tyr) under a strong constitutive PGK1pr
    Depositor
    Sahand Jamal Rahi
    Article
    Gligorovski et al bioRxiv 2024.02.28.582517
    Insert
    EL222 codon-optimized for budding yeast
    Use
    Tags
    Expression
    Yeast
    Mutation
    Asp89Tyr mutant
    Promoter
    PGK1pr
    Available since
    Jan. 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    227752-plasmid-map-sequence-id-458027

    Enlarge

  23. pVG137

    Plasmid
    #227751
    Purpose
    Expresses a version of light-inducible transcription factor EL222 (Val201Leu) under a strong constitutive PGK1pr
    Depositor
    Sahand Jamal Rahi
    Article
    Gligorovski et al bioRxiv 2024.02.28.582517
    Insert
    EL222 codon-optimized for budding yeast
    Use
    Tags
    Expression
    Yeast
    Mutation
    Val201Leu mutant
    Promoter
    PGK1pr
    Available since
    Jan. 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    227751-plasmid-map-sequence-id-458026

    Enlarge

  24. pVG136

    Plasmid
    #227750
    Purpose
    Expresses a version of light-inducible transcription factor EL222 (Pro165Ser) under a strong constitutive PGK1pr
    Depositor
    Sahand Jamal Rahi
    Article
    Gligorovski et al bioRxiv 2024.02.28.582517
    Insert
    EL222 codon-optimized for budding yeast
    Use
    Tags
    Expression
    Yeast
    Mutation
    Pro165Ser mutant
    Promoter
    PGK1pr
    Available since
    Jan. 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    227750-plasmid-map-sequence-id-458025

    Enlarge

  25. pVG135

    Plasmid
    #227749
    Purpose
    Expresses a version of light-inducible transcription factor EL222 (Lys99Glu) under a strong constitutive PGK1pr
    Depositor
    Sahand Jamal Rahi
    Article
    Gligorovski et al bioRxiv 2024.02.28.582517
    Insert
    EL222 codon-optimized for budding yeast
    Use
    Tags
    Expression
    Yeast
    Mutation
    Lys99Glu mutant
    Promoter
    PGK1pr
    Available since
    Jan. 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    227749-plasmid-map-sequence-id-458022

    Enlarge

  26. pVG119

    Plasmid
    #227748
    Purpose
    Expresses a version of light-inducible transcription factor EL222 (Val139Leu) under a strong constitutive PGK1pr
    Depositor
    Sahand Jamal Rahi
    Article
    Gligorovski et al bioRxiv 2024.02.28.582517
    Insert
    EL222 codon-optimized for budding yeast
    Use
    Tags
    Expression
    Yeast
    Mutation
    Val139Leu mutant
    Promoter
    PGK1pr
    Available since
    Jan. 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    227748-plasmid-map-sequence-id-458021

    Enlarge

  27. pVG118

    Plasmid
    #227747
    Purpose
    Expresses a version of light-inducible transcription factor EL222 (Asp18Tyr) under a strong constitutive PGK1pr
    Depositor
    Sahand Jamal Rahi
    Article
    Gligorovski et al bioRxiv 2024.02.28.582517
    Insert
    EL222 codon-optimized for budding yeast
    Use
    Tags
    Expression
    Yeast
    Mutation
    Asp18Tyr mutant
    Promoter
    PGK1pr
    Available since
    Jan. 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    227747-plasmid-map-sequence-id-458020

    Enlarge

  28. pVG117

    Plasmid
    #227746
    Purpose
    Expresses a version of light-inducible transcription factor EL222 (Asp18Tyr, Val139Leu) under a strong constitutive PGK1pr
    Depositor
    Sahand Jamal Rahi
    Article
    Gligorovski et al bioRxiv 2024.02.28.582517
    Insert
    EL222 codon-optimized for budding yeast
    Use
    Tags
    Expression
    Yeast
    Mutation
    Asp18Tyr, Val139Leu double mutant
    Promoter
    PGK1pr
    Available since
    Jan. 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    227746-plasmid-map-sequence-id-458019

    Enlarge

  33. pVH613

    Plasmid
    #169602
    Purpose
    Tier-2 vector encoding PCREm4-driven Nluc and PmPGK1-driven Fluc expression (PCREm4-Nluc-pA::A2-pA::PmPGK1-Fluc-pA).
    Depositor
    Martin Fussenegger
    Article
    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10
    Insert
    cAMP response element-controlled NanoLuc Luciferase expression cassette
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    PCREm4 / PPGK
    Available since
    June 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    169602-plasmid-map-sequence-id-365597

    Enlarge

  35. pTS2163

    Plasmid
    #169632
    Purpose
    Tier-3 vector for CRISPR-mediated stable integration into the AAVS1 locus of the tetON-system (OTetO7-PCMVmin-SEAP-p2A-iRFP670-pA::PmPGK1-rtTA-pA::A3-pA)
    Depositor
    Martin Fussenegger
    Article
    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10
    Insert
    OTetO7-PCMVmin-SEAP-iRFP670::PmPGK1-rtTA-pA::A3
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    tetO7-PhCMVmin / PPGK
    Available since
    July 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    169632-plasmid-map-sequence-id-366785

    Enlarge

  39. JPF0507

    Plasmid
    #123998
    Purpose
    Encodes pPGK1 driving expression of mAzamiGreen with an mScarlet normalization in a PiggyBac destination vector
    Depositor
    Hana El-Samad
    Article
    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2
    Insert
    PB_pPGK1-NLS-mAzamiGreen-bghpA_pCAG-H2B::mScarlet-rglpA
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    123998-plasmid-map-sequence-id-252307

    Enlarge

  40. JPF0521

    Plasmid
    #124012
    Purpose
    Encodes crippled pPGK1 driving expression of mAzamiGreen with an mScarlet normalization in a PiggyBac destination vector
    Depositor
    Hana El-Samad
    Article
    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2
    Insert
    PB_pPGK1c-NLS-mAzamiGreen-bghpA_pCAG-H2B::mScarlet-rglpA
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    124012-plasmid-map-sequence-id-252423

    Enlarge

Showing: 121 - 160 of 234 results