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Plasmid#208067PurposeExpression of GFP-tagged hsRPA1 in human cellsDepositorInsertRPA1 (RPA1 Human)
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceDec. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pmVen-RPA70-IRES-RPA32-IRES-RPA14
Plasmid#211431Purposemammalian expression of human RPA70 N-terminally fused to mVenus and RPA32 and RPA14DepositorUseTagsmVenus (on RPA70)ExpressionMammalianMutationPromoterAvailable sinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRPA70-IRES-Ven-RPA32-IRES-RPA14
Plasmid#211430Purposemammalian expression of human RPA32 N-terminally fused to mVenus and RPA70 and RPA14DepositorUseTagsmVenus (on RPA32)ExpressionMammalianMutationPromoterAvailable sinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
p11d-tRPA-32Ala9
Plasmid#102616PurposeExpression of human RPA with an alanine substituted phosphorylation domain (with the following mutations in RPA32 :S8,11-13,23,29,33,39A,T21A).DepositorUseTagsExpressionBacterialMutationwith an alanine substituted phosphorylation domainPromoterT7Available sinceNov. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_RPA1_RepA-N
Plasmid#110032PurposeProtein expression and purification of RPA1_RepA-NDepositorInsertRPA1_RepA-N (RPA1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
p11d-RPA70/14 (MSW#134)
Plasmid#208076PurposeExpression of human Replication Protein A 70 and 14-kDa subunits in E. coli with 14 having a His-tagDepositorUseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPMVAd3
Plasmid#98298PurposeEnhancing the lower-part mevalonate pathway; overexpressing yeast mevalonate pathway genes under the control of consitutive promoters.DepositorInsertPRPL8B-ERG12-TNAT5-PSSB1-ERG8-TIDP1-PRPL3-MVD1-TIDP1-PYEF3-IDI1-TRPL15A
UseTagsExpressionYeastMutationPromoterAvailable sinceAug. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMXs-DDX3X
Plasmid#70648PurposeExpresses WT DDX3X in mammalian cells under blasticidin selection.DepositorInsertDDX3X (DDX3X Human)
UseRetroviralTags3x FLAGExpressionMammalianMutationPromoterAvailable sinceApril 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
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p11d-tRPA(123)
Plasmid#102613PurposeExpression of human RPA in E. coliDepositorUseTagsExpressionBacterialMutationPromoterT7Available sinceNov. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
p11d-aRPA (MSW#712)
Plasmid#208078PurposeExpression of Alternative human Replication Protein A (RPA1,4,3) in E. coliDepositorUseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
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TFORF1345
Plasmid#142788PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertRPA1 (RPA1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
p11d-tRPA (MSW#111)
Plasmid#208066Purposeexpression of human Replication Protein A in E. coliDepositorUseTagsExpressionBacterialMutationRPA32 has a point mutation Thr88(ACC) -> Ile(A…PromoterAvailable sinceNov. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pColdI-DDX3_helicase_core
Plasmid#233618PurposeExpression of His-tagged helicase core region of DDX3X in E.coliDepositorInsertDDX3X helicase core WT (DDX3X Human)
UseTagsHis6ExpressionBacterialMutationPromoterAvailable sinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAPM-miR30-DDX3X-ts1
Plasmid#174235PurposeDDX3X knockdownDepositorInsertDDX3X shRNA (DDX3X Human)
UseLentiviral and RNAiTagsExpressionMutationPromoterSFFVAvailable sinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAPM-miR30-DDX3X-ts2
Plasmid#174236PurposeDDX3X knockdownDepositorInsertDDX3X shRNA (DDX3X Human)
UseLentiviral and RNAiTagsExpressionMutationPromoterSFFVAvailable sinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAPM-miR30-DDX3X-ts3
Plasmid#174237PurposeDDX3X knockdownDepositorInsertDDX3X shRNA (DDX3X Human)
UseLentiviral and RNAiTagsExpressionMutationPromoterSFFVAvailable sinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
p11d-tRPA-32Asp8
Plasmid#102617PurposeExpression of human RPA with aspartic acid substituted phosphorylation domainDepositorUseTagsExpressionBacterialMutationwith aspartic acid substituted phosphorylation do…PromoterAvailable sinceNov. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pColdI-DDX3_helicase_core_Q360L
Plasmid#233619PurposeExpression of His-tagged helicase core region of DDX3X with Q360L mutation in E.coliDepositorInsertDDX3X helicase core Gln360Leu (DDX3X Human)
UseTagsHis6ExpressionBacterialMutationGln360LeuPromoterAvailable sinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pColdI-DDX3_helicase_core_Q360P
Plasmid#233620PurposeExpression of His-tagged helicase core region of DDX3X with Q360P mutation in E.coliDepositorInsertDDX3X helicase core Gln360Pro (DDX3X Human)
UseTagsHis6ExpressionBacterialMutationGln360ProPromoterAvailable sinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
CL20_mEGFP_DDX3X_MLLT10
Plasmid#205802PurposeExpress mEGFP-tagged fusion protein, DDX3X_MLLT10 from patient-derived sequenceDepositorUseTagsmEGFPExpressionMammalianMutationPromoterAvailable sinceNov. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-DDX3X
Plasmid#116730PurposeLentiviral expression of DDX3XDepositorInsertDDX3X (DDX3X Human)
UseLentiviralTagsExpressionMutationWTPromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-DDX3X-S412F
Plasmid#116227PurposeLentiviral expression of DDX3X S412FDepositorInsertDDX3X (DDX3X Human)
UseLentiviralTagsExpressionMutationS412FPromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-DDX3X-E285K
Plasmid#116226PurposeLentiviral expression of DDX3X E285KDepositorInsertDDX3X (DDX3X Human)
UseLentiviralTagsExpressionMutationE285KPromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
CL20_mEGFP_DDX3X_MRAS
Plasmid#205803PurposeExpress mEGFP-tagged fusion protein, DDX3X_MRAS from patient-derived sequenceDepositorUseTagsmEGFPExpressionMammalianMutationPromoterAvailable sinceNov. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL4.23 C3_26
Plasmid#60306PurposeThis plasmid contains a human pancreatic islet active enhancer, a minimal promoter and a luc2 gene.DepositorInsertOBFC2A enhancer (NABP1 Human)
UseLuciferaseTagsLuciferaseExpressionMammalianMutationPromoterAvailable sinceDec. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pENTR D-TOPO-C3_26
Plasmid#60269PurposeThis plasmid contains a human pancreatic islet active enhancer, which can be shuttled into a Gateway destination vector, such as pGL4.23-Gateway.DepositorInsertOBFC2A enhancer (NABP1 Human)
UseEntry vectorTagsExpressionMutationPromoterAvailable sinceDec. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
BIC-Gag-DDX3
Plasmid#233687PurposeExpresses a fusion between the Murine Leukemia Virus Gag polyprotein and the human DDX3 protein to produce virus-like particles loaded with DDX3 and deliver it to target cells.DepositorInsertDEAD-box helicase 3 (DDX3X Human)
UseRetroviralTagsGag (Murine Leukemia Virus)ExpressionMammalianMutationPromoterhCMVAvailable sinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only