We narrowed to 190 results for: Vibrio

1. pSL0812 (pCRISPR(BbsI)_Vch)

   Plasmid

   #200898

   Purpose

   Expresses V. cholerae CRISPR RNA from a U6 promoter. The CRISPR array contains two BbsI sites for spacer cloning.

   Depositor

   Samuel H. Sternberg

   Article

   Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

   Insert

   VchCRISPR (entry)
   

   Expression

   Mammalian

   Available Since

   May 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSL0747 (pDonor_L-MmeI)

   Plasmid

   #130647

   Purpose

   Encodes a mini-transposon derived from V. cholerae Tn6677 CAST, with CmR cargo gene. The mutated left end introduces an MmeI recognition site (used for Tn-seq). Total transposon size = 977 bp.

   Depositor

   Samuel H. Sternberg

   Article

   Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:

   Insert

   VchCAST donor DNA (L*)
   

   Use

   CRISPR; Transposon

   Expression

   Bacterial

   Mutation

   TGTTGATG --> TGTTGGAG

   Available Since

   Sept. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSL0746 (pDonor_R-MmeI)

   Plasmid

   #130646

   Purpose

   Encodes a mini-transposon derived from V. cholerae Tn6677 CAST, with CmR cargo gene. The mutated right end introduces an MmeI recognition site (used for Tn-seq). Total transposon size = 977 bp.

   Depositor

   Samuel H. Sternberg

   Article

   Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:

   Insert

   VchCAST donor DNA (R*)
   

   Use

   CRISPR; Transposon

   Expression

   Bacterial

   Mutation

   TGTTGATA --> TGTTGGAA

   Available Since

   Sept. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFE-vcDAB

   Plasmid

   #133004

   Purpose

   pFE carrying the vcdabA2 ( locus_tag: CSW01_RS07960) and vcdabB2 genes (locus_tag: CSW01_RS07955)

   Depositor

   David Savage

   Article

   Desmarais et al Nat Microbiol. 2019 Aug 12. pii: 10.1038

   Inserts

   vcdabB
   vcdabA
   

   Expression

   Bacterial

   Promoter

   tetR

   Available Since

   Nov. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pVRa11_987

   Plasmid

   #49661

   Depositor

   Christopher Voigt

   Article

   Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

   Insert

   ECF11_987
   

   Use

   Synthetic Biology

   Tags

   His6-PreScission

   Expression

   Bacterial

   Mutation

   Codon optimized for E.coli

   Promoter

   pT7-lacO

   Available Since

   Jan. 3, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. MZB127

   Plasmid

   #217836

   Purpose

   Bacterial expression of human Arf1 (Q71L) with C-terminal 'self-cleaving' CPD-10xHis tag

   Depositor

   Richard Baker

   Article

   Begley et al Proc Natl Acad Sci U S A. 2024 Dec 24;12

   Insert

   Arf1, rtxA (ARF1 Vibrio cholerae, Human)
   

   Tags

   10x His and CPD

   Expression

   Bacterial

   Mutation

   Q71L

   Available Since

   May 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCgVchDonor

   Plasmid

   #203817

   Purpose

   DNA donor for transposition, shuttle vector for Corynebacterium glutamicum / E. coli

   Depositor

   Sheng Yang

   Article

   Yang et al ACS Synth Biol. 2023 Jul 21;12(7):2198-2

   Insert

   Mini-Tn, Spec cassette
   

   Use

   CRISPR

   Expression

   Bacterial

   Available Since

   Aug. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCVD619

   Plasmid

   #66161

   Purpose

   heat-stable enterotoxin from parent strain Vibrio cholera NRT36S

   Depositor

   James Kaper

   Insert

   nag-ST
   

   Use

   Cloning vector

   Promoter

   Unknown

   Available Since

   July 9, 2015

   Availability

   Academic Institutions and Nonprofits only

9. pBsVchCAST-pyrE

   Plasmid

   #203813

   Purpose

   Expression of CRISPR-associated transposases, shuttle vector for Bacillus subtilis / E. coli

   Depositor

   Sheng Yang

   Article

   Yang et al ACS Synth Biol. 2023 Jul 21;12(7):2198-2

   Insert

   VchTniQ, VchCas5/8, VchCas7, VchCas6, VchTnsABC, CRISPR(pyrE)
   

   Use

   CRISPR

   Expression

   Bacterial

   Available Since

   Aug. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pST_140_LVL2 cam

    Plasmid

    #179334

    Purpose

    NT-CRISPR plasmid for a single gRNA with spG Cas9 (near PAM-less Cas9 variant).

    Depositor

    Anke Becker

    Article

    Stukenberg et al Commun Biol. 2022 Mar 25;5(1):265. doi:

    Insert

    Ptac tfoX, Ptet spG cas9, PJ23106 acrIIA4, Ptet gRNA with sfGFP dropout
    

    Use

    CRISPR

    Mutation

    Cas9 -> SpG Cas9 (D1135L, S1136W, G1218K, E121…

    Available Since

    March 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pQCascade

    Plasmid

    #140622

    Purpose

    Expresses V. cholerae TniQ, Cas8, Cas7, and Cas6 and CRISPR RNA

    Depositor

    Sheng Yang

    Article

    Zhang et al ACS Synth Biol. 2020 Jun 17. doi: 10.102

    Insert

    VchTniQ, VchCas8, VchCas7, VchCas6, CRISPR(entry)
    

    Use

    CRISPR; Transposon

    Expression

    Bacterial

    Available Since

    July 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBsVchCAST

    Plasmid

    #203812

    Purpose

    Expression of CRISPR-associated transposases, shuttle vector for Bacillus subtilis / E. coli

    Depositor

    Sheng Yang

    Article

    Yang et al ACS Synth Biol. 2023 Jul 21;12(7):2198-2

    Insert

    VchTniQ, VchCas5/8, VchCas7, VchCas6, VchTnsABC
    

    Use

    CRISPR

    Expression

    Bacterial

    Available Since

    Aug. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCgVchCAST-crtYf

    Plasmid

    #203816

    Purpose

    Expression of CRISPR-associated transposases, shuttle vector for Corynebacterium glutamicum / E. coli

    Depositor

    Sheng Yang

    Article

    Yang et al ACS Synth Biol. 2023 Jul 21;12(7):2198-2

    Insert

    VchTniQ, VchCas5/8, VchCas7, VchCas6, VchTnsABC, CRISPR(crtYf)
    

    Use

    CRISPR

    Expression

    Bacterial

    Available Since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDonor-GDH

    Plasmid

    #140629

    Purpose

    Encodes a mini-transposon derived from V. cholerae Tn6677 and T7-GDH cargo gene.

    Depositor

    Sheng Yang

    Article

    Zhang et al ACS Synth Biol. 2020 Jun 17. doi: 10.102

    Insert

    Mini-Tn6677, T7lac-GDH gene cassette
    

    Use

    CRISPR; Transposon

    Expression

    Bacterial

    Available Since

    July 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pQCascade-array1

    Plasmid

    #140623

    Purpose

    Expresses V. cholerae TniQ, Cas8, Cas7, and Cas6 and crRNA-array1. The crRNA-array1 targets 8 different loci in Escherchia coli.

    Depositor

    Sheng Yang

    Article

    Zhang et al ACS Synth Biol. 2020 Jun 17. doi: 10.102

    Insert

    VchTniQ, VchCas8, VchCas7, VchCas6, crRNA-array1
    

    Use

    CRISPR; Transposon

    Expression

    Bacterial

    Available Since

    July 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pQCascade-IS1

    Plasmid

    #140624

    Purpose

    Expresses V. cholerae TniQ, Cas8, Cas7, and Cas6 and crRNA-IS1. The crRNA-IS1 targets IS1 loci in Escherchia coli.

    Depositor

    Sheng Yang

    Article

    Zhang et al ACS Synth Biol. 2020 Jun 17. doi: 10.102

    Insert

    VchTniQ, VchCas8, VchCas7, VchCas6, crRNA-IS1
    

    Use

    CRISPR; Transposon

    Expression

    Bacterial

    Available Since

    July 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pQCascade-IS186

    Plasmid

    #140627

    Purpose

    Expresses V. cholerae TniQ, Cas8, Cas7, and Cas6 and crRNA-IS186. The crRNA-IS186 targets IS186 loci in Escherchia coli.

    Depositor

    Sheng Yang

    Article

    Zhang et al ACS Synth Biol. 2020 Jun 17. doi: 10.102

    Insert

    VchTniQ, VchCas8, VchCas7, VchCas6, crRNA-IS186
    

    Use

    CRISPR; Transposon

    Expression

    Bacterial

    Available Since

    Sept. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pQCascade-IS3

    Plasmid

    #140626

    Purpose

    Expresses V. cholerae TniQ, Cas8, Cas7, and Cas6 and crRNA-IS3. The crRNA-IS3 targets IS3 loci in Escherchia coli.

    Depositor

    Sheng Yang

    Article

    Zhang et al ACS Synth Biol. 2020 Jun 17. doi: 10.102

    Insert

    VchTniQ, VchCas8, VchCas7, VchCas6, crRNA-IS3
    

    Use

    CRISPR; Transposon

    Expression

    Bacterial

    Available Since

    July 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pQCascade-IS2

    Plasmid

    #140625

    Purpose

    Expresses V. cholerae TniQ, Cas8, Cas7, and Cas6 and crRNA-IS2. The crRNA-IS2 targets IS2 loci in Escherchia coli.

    Depositor

    Sheng Yang

    Article

    Zhang et al ACS Synth Biol. 2020 Jun 17. doi: 10.102

    Insert

    VchTniQ, VchCas8, VchCas7, VchCas6, crRNA-IS2
    

    Use

    CRISPR; Transposon

    Expression

    Bacterial

    Available Since

    July 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pQCascade-tra13

    Plasmid

    #140628

    Purpose

    Expresses V. cholerae TniQ, Cas8, Cas7, and Cas6 and crRNA-tra13. The crRNA-tra13 targets 13 copies transposon loci in Tatumella citrea.

    Depositor

    Sheng Yang

    Article

    Zhang et al ACS Synth Biol. 2020 Jun 17. doi: 10.102

    Insert

    VchTniQ, VchCas8, VchCas7, VchCas6, crRNA-tra13
    

    Use

    CRISPR; Transposon

    Expression

    Bacterial

    Available Since

    July 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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21. pFP.E222

    Plasmid

    #138248

    Purpose

    Three input / three output logic circuit plasmid - Expresses different intein split ECFs from different inducible promoters

    Depositor

    Baojun Wang

    Article

    Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:

    Inserts

    araC (araC )
    ECF20.N.(VDA).M86.N2
    Terminator
    SspGyrB.C2.(SAK).ECF16.C
    Terminator
    rhaS
    ECF17.N.(NPC).NrdJ-1.N2
    Terminator
    M86.C2.(SDL).ECF20.C
    Terminator
    luxR
    Terminator
    ECF16.N.(AGG).SspGyrB.N2
    Terminator
    NrdJ-1.C2.(SEI).ECF17.C
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    J23115, P(araBAD), P(lux2), P(rhaBAD), iJ23101, a…

    Available Since

    March 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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22. pBFC0619

    Plasmid

    #186690

    Purpose

    VcDART under control of Lac promoter, Vc_2xBsaI_NT gRNA, GmR+sfGFP cargo

    Depositor

    Jennifer Doudna

    Article

    Rubin et al Nat Microbiol. 2022 Jan;7(1):34-47. doi:

    Inserts

    tnsA
    tnsB
    tnsC
    tnsD
    tniQ
    cas8-cas5 fusion
    cas7
    cas6
    Vc_2xBsaI_NT (Guide Stuffer) crRNA
    Tn7 transposon
    GmR+sfGFP VcDART Cargo
    

    Expression

    Bacterial

    Promoter

    PLac

    Available Since

    Sept. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pBFC0996

    Plasmid

    #186695

    Purpose

    VcDART under control of Lac promoter, Vc_2xBsaI_NT gRNA, 2xLguI cargo stuffer, with ET-Seq (AsiSI+SbfI) restriction sites flanking Tn7 Right End

    Depositor

    Jennifer Doudna

    Article

    Rubin et al Nat Microbiol. 2022 Jan;7(1):34-47. doi:

    Inserts

    tnsA
    tnsB
    tnsC
    tnsD
    tniQ
    cas8-cas5 fusion
    cas7
    cas6
    crRNA
    Tn7 transposon
    2xLguI Cargo Stuffer
    SbfI+AsiSI dual restriction site for donor plasmid removal during ET-Seq
    

    Expression

    Bacterial

    Promoter

    PLac

    Available Since

    Sept. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pBFC0625

    Plasmid

    #186691

    Purpose

    VcDART under control of Lac promoter, Vc_lacZ_α_1 gRNA, GmR+sfGFP cargo

    Depositor

    Jennifer Doudna

    Article

    Rubin et al Nat Microbiol. 2022 Jan;7(1):34-47. doi:

    Inserts

    tnsA
    tnsB
    tnsC
    tnsD
    tniQ
    cas8-cas5 fusion
    cas7
    cas6
    Vc_lacZ_α_1 Spacer crRNA
    Tn7 transposon
    GmR+sfGFP VcDART Cargo
    

    Expression

    Bacterial

    Promoter

    PLac

    Available Since

    Sept. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pBFC1207

    Plasmid

    #186694

    Purpose

    VcDART under control of Lac promoter, Vc_2xBsaI_NT gRNA, GmR+sfGFP cargo, with ET-Seq (AsiSI+SbfI) restriction sites flanking Tn7 Right End

    Depositor

    Jennifer Doudna

    Article

    Rubin et al Nat Microbiol. 2022 Jan;7(1):34-47. doi:

    Inserts

    tnsA
    tnsB
    tnsC
    tnsD
    tniQ
    cas8-cas5 fusion
    cas7
    cas6
    Vc_2xBsaI_NT (Guide Stuffer) crRNA
    Tn7 transposon
    GmR+sfGFP VcDART Cargo
    SbfI+AsiSI dual restriction site for donor plasmid removal during ET-Seq
    

    Expression

    Bacterial

    Promoter

    PLac

    Available Since

    Sept. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pRL1063a

    Plasmid

    #70687

    Purpose

    pRL1058 with Vibrio fischeri luxAB for sensing

    Depositor

    Peter Wolk

    Article

    Wolk et al Proc Natl Acad Sci U S A. 1991 Jun 15;88

    Insert

    Transposon Tn5-1063
    

    Available Since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pET22b-CPDSalI

    Plasmid

    #38251

    Depositor

    Aimee Shen, Matthew Bogyo

    Article

    Shen et al PLoS One. 2009 Dec 2;4(12):e8119.

    Type

    Empty backbone

    Tags

    6xHis and Vibrio cholerae MARTX toxin cysteine pr…

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 19, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pET22b-CPDBamHI-Leu

    Plasmid

    #38256

    Depositor

    Aimee Shen, Matthew Bogyo

    Article

    Shen et al PLoS One. 2009 Dec 2;4(12):e8119.

    Type

    Empty backbone

    Tags

    6xHis and Vibrio cholerae MARTX toxin cysteine pr…

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 1, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pET28a-CPDSalI

    Plasmid

    #38252

    Depositor

    Aimee Shen, Matthew Bogyo

    Article

    Shen et al PLoS One. 2009 Dec 2;4(12):e8119.

    Type

    Empty backbone

    Tags

    6xHis and Vibrio cholerae MARTX toxin cysteine pr…

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 2, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pET22b-CPDSacI

    Plasmid

    #38255

    Depositor

    Aimee Shen, Matthew Bogyo

    Article

    Shen et al PLoS One. 2009 Dec 2;4(12):e8119.

    Type

    Empty backbone

    Tags

    6xHis and Vibrio cholerae MARTX toxin cysteine pr…

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 19, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pET28a-HA-CPDSalI

    Plasmid

    #38254

    Depositor

    Aimee Shen, Matthew Bogyo

    Article

    Shen et al PLoS One. 2009 Dec 2;4(12):e8119.

    Type

    Empty backbone

    Tags

    6xHis, HA, and Vibrio cholerae MARTX toxin cystei…

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 2, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pET22b-HA-CPDSalI

    Plasmid

    #38253

    Depositor

    Aimee Shen, Matthew Bogyo

    Article

    Shen et al PLoS One. 2009 Dec 2;4(12):e8119.

    Type

    Empty backbone

    Tags

    6xHis, HA, and Vibrio cholerae MARTX toxin cystei…

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 19, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pDropVn

    Plasmid

    #184873

    Purpose

    Chromosomal transfer helper plasmid with sgRNAs (one fixed, one flexible) for Vibrio natriegens genome editing protocol.

    Depositor

    Patrick Sobetzko

    Article

    Teufel et al Nat Commun. 2022 Jun 14;13(1):3430. doi:

    Insert

    lacZalpha,ccdB
    

    Expression

    Bacterial

    Available Since

    Jan. 3, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

34. pSB1C3-Lux

    Plasmid

    #109383

    Purpose

    Arabinose inducible expression of the Vibrio Fischery Lux operon. Used to test burden response in E. coli

    Depositor

    Tom Ellis

    Article

    Ceroni et al Nat Methods. 2018 Mar 26. pii: nmeth.463

    Insert

    pARABAD-LUX operon
    

    Expression

    Bacterial

    Available Since

    May 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pQdCas9-sgempty

    Plasmid

    #159523

    Purpose

    Expression of dCas9 driven by lux cassette from Vibrio fischeri

    Depositor

    Radhakrishnan Mahadevan

    Article

    Liu et al ACS Synth Biol. 2020 Jun 5. doi: 10.1021

    Insert

    quorum sensing cassette luxI, luxR, and the LuxR-dependent promoter pLuxI , dcas9 and sgRNA
    

    Use

    CRISPR

    Promoter

    quorum sensing promoter

    Available Since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pET22b-GFP-CPDSalI

    Plasmid

    #38257

    Depositor

    Aimee Shen, Matthew Bogyo

    Article

    Shen et al PLoS One. 2009 Dec 2;4(12):e8119.

    Insert

    eGFP
    

    Tags

    6xHis and Vibrio cholerae MARTX toxin cysteine pr…

    Expression

    Bacterial

    Mutation

    cloned from pEGFPN3 (Clonetech)

    Promoter

    T7

    Available Since

    July 20, 2012

    Availability

    Academic Institutions and Nonprofits only

37. pET22b-BirA-CPDSalI

    Plasmid

    #38258

    Depositor

    Aimee Shen, Matthew Bogyo

    Article

    Shen et al PLoS One. 2009 Dec 2;4(12):e8119.

    Insert

    birA (birA E. coli)
    

    Tags

    6xHis and Vibrio cholerae MARTX toxin cysteine pr…

    Expression

    Bacterial

    Mutation

    cloned from pGEX4T1-BirA

    Promoter

    T7

    Available Since

    July 20, 2012

    Availability

    Academic Institutions and Nonprofits only

38. pRK5-vox-EGFP

    Plasmid

    #79970

    Purpose

    mammalian GFP reporter plasmid, target site for Vika recombinase

    Depositor

    Frank Buchholz

    Article

    Karimova et al Nucleic Acids Res. 2013 Jan;41(2):e37. d

    Insert

    2 vox-sites flanking neo in frame with EGFP
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pC0.062

    Plasmid

    #119651

    Purpose

    Level 0 part. Terminator for luxICDABEG operon from Vibrio fischeri.

    Depositor

    Alistair McCormick

    Article

    Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

    Insert

    Bba_B0011
    

    Use

    Synthetic Biology

    Available Since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pNPK-NLS-Vika

    Plasmid

    #79969

    Purpose

    expresses human codon-optimized Vika-recombinase in mammalian cells

    Depositor

    Frank Buchholz

    Article

    Karimova et al Nucleic Acids Res. 2013 Jan;41(2):e37. d

    Insert

    human codon-optimized Vika sequence, NLS tagged
    

    Tags

    NLS

    Expression

    Mammalian

    Promoter

    PGK

    Available Since

    Aug. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge