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Showing: 151 - 200 of 18428 results

  2. pDIRECT_25I

    Plasmid
    #91146
    Purpose
    Direct Cloning, Type: T-DNA, Engineering Reagent: ZmUbi:Csy4-P2A-TaCas9_dead + PvUbi1:gRNAs with Csy4 spacers, Plant Selection: PvUbi2:hpt II
    Depositor
    Daniel Voytas
    Article
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Insert
    Engineering Reagent: ZmUbi:Csy4-P2A-TaCas9_dead + PvUbi1:gRNAs with Csy4 spacers
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    D10A, H840A
    Promoter
    Available since
    July 11, 2017
    Availability
    Academic Institutions and Nonprofits only
    91146-plasmid-map-sequence-id-182476

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  7. pDIRECT_22D

    Plasmid
    #91136
    Purpose
    Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:Csy4-P2A-AtCas9_dead + CmYLCV:gRNAs with Csy4 spacers, Plant Selection: 2x35S:npt II
    Depositor
    Daniel Voytas
    Article
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Insert
    Engineering Reagent: 35S:Csy4-P2A-AtCas9_dead + CmYLCV:gRNAs with Csy4 spacers
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    D10A, H840A
    Promoter
    Available since
    July 11, 2017
    Availability
    Academic Institutions and Nonprofits only
    91136-plasmid-map-sequence-id-182475

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  8. pDIRECT_21D

    Plasmid
    #91131
    Purpose
    Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:Csy4-P2A-AtCas9_dead + CmYLCV:gRNAs with Csy4 spacers, Plant Selection: 2x35S:hpt II
    Depositor
    Daniel Voytas
    Article
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Insert
    Engineering Reagent: 35S:Csy4-P2A-AtCas9_dead + CmYLCV:gRNAs with Csy4 spacers
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    D10A, H840A
    Promoter
    Available since
    July 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    91131-plasmid-map-sequence-id-182255

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  22. IRE1bpBS

    Plasmid
    #21878
    Depositor
    David Ron
    Insert
    IRE1b (Ern2 Mouse)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Sept. 10, 2009
    Availability
    Academic Institutions and Nonprofits only
    21878-plasmid-map-sequence-id-10059

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  23. pHR_SFFV_tTA_IRES_tagBFP

    Plasmid
    #231953
    Purpose
    Lentiviral vector for constitutive expression of the tetracycline transactivator tTA and of the blue fluorescent protein tagBFP, separated by an internal ribosome entry site (SFFV promoter)
    Depositor
    Leonardo Morsut
    Insert
    tTA_IRES_tagBFP
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    231953-plasmid-map-sequence-id-470256

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  35. pASPIre4

    Plasmid
    #196656
    Purpose
    Derivative of pASPIre3. Contains SpeI restriction site within the CDS of bxb1 to enable diversification of the 5’-UTR and codons 2-16.
    Depositor
    Markus Jeschek
    Article
    Hollerer et al Nucleic Acids Res. 2023 Mar 21;51(5):237
    Insert
    Bxb1-sfGFP fusion controlled by rhamnose promoter; attB/attP-flanked discriminator; exchangable 5'-UTR and CDS (codons 1-16)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    SpeI site in Bxb1 CDS
    Promoter
    rhamnose-inducible promoter
    Available since
    Aug. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    196656-plasmid-map-sequence-id-403168

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  38. pH-Sapphire

    Plasmid
    #176566
    Purpose
    Expression of Sapphire for auxotrophic selection in the absence of lysine
    Depositor
    Markus Ralser
    Insert
    yETSapphire
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    TDH1(GAP)
    Available since
    Oct. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    176566-plasmid-map-sequence-id-350974

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  39. pU-Sapphire

    Plasmid
    #176567
    Purpose
    Expression of Sapphire for auxotrophic selection in the absence of uracil
    Depositor
    Markus Ralser
    Insert
    yETSapphire
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    TDH1(GAP)
    Available since
    Oct. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    176567-plasmid-map-sequence-id-353606

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  40. pM-Sapphire

    Plasmid
    #176569
    Purpose
    Expression of Sapphire for auxotrophic selection in the absence of methionine
    Depositor
    Markus Ralser
    Insert
    yETSapphire
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    TDH1(GAP)
    Available since
    Oct. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    176569-plasmid-map-sequence-id-350302

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  44. miReporter-PGK

    Plasmid
    #82477
    Purpose
    microRNA reporter relying on a bidirectional PGK promoter. Contains MCS downstream of H2B-mCherry and H2B-Citrine to insert miRNA binding sites. Contains a Hygromycin selection cassette.
    Depositor
    Pierre Neveu
    Article
    Sladitschek et al PLoS One. 2016 May 6;11(5):e0155177. doi
    Insert
    H2B-mCherry and H2B-Citrine. HygroR
    Use
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    82477-plasmid-map-sequence-id-163461

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  46. CoV2-M-IRES-E

    Plasmid
    #177938
    Purpose
    Transient mammalian expression of codon optimized SARS-CoV-2 Membrane and Envelope proteins (original Wuhan Hu1 sequence). Used for generating RNA packaging virus-like particles.
    Depositor
    Jennifer Doudna
    Article
    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11
    Insert
    SARS-CoV-2 Membrane and Envelope proteins (original Wuhan Hu1 sequence) (E, M )
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    177938-plasmid-map-sequence-id-429078

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  49. miReporter-CAG

    Plasmid
    #82478
    Purpose
    microRNA reporter relying on a bidirectional CAG-based promoter. Contains MCS downstream of H2B-mCherry and H2B-Citrine to insert miRNA binding sites. Contains a Hygromycin selection cassette.
    Depositor
    Pierre Neveu
    Article
    Sladitschek et al PLoS One. 2016 May 6;11(5):e0155177. doi
    Insert
    H2B-mCherry and H2B-Citrine. HygroR
    Use
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    82478-plasmid-map-sequence-id-174379

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Showing: 151 - 200 of 18428 results