We narrowed to 1,293 results for: agl

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Fragment1 with TLS2

Plasmid

#196983

Purpose

Gateway-compatible gRNA backbone with TLS2 mobility signal. Template for the addition of target sequences to produce 2x graft-mobile gRNA-TLS2.

Depositor

Friedrich Kragler

Article

Yang et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

Intermediate fragment for assembly of gRNA-TLS2 construct

Use

Gateway-compatible entry vector

Available Since

May 12, 2023

Availability

Academic Institutions and Nonprofits only

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pHIV-SapI-LacZ

Plasmid

#237996

Purpose

Expression plasmid for transfer of complete TCR library from pGGA backbone

Depositor

Michael Birnbaum

Article

Gaglione et al bioRxiv 2025.04.24.649726

Type

Empty backbone

Use

Lentiviral

Available Since

July 17, 2025

Availability

Academic Institutions and Nonprofits only

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pUSE-Src-YF-UniRapR-mCerulean-myc

Plasmid

#45381

Depositor

Klaus Hahn

Article

Dagliyan et al Proc Natl Acad Sci U S A. 2013 Apr 23;11

Insert

Src-YF-UniRapR-mCerulean-myc

Tags

Myc, Myr, and mCerulean

Expression

Mammalian

Mutation

changed mSrc Tyrosine 529 to Phenylalanine

Promoter

CMV

Available Since

Nov. 7, 2013

Availability

Academic Institutions and Nonprofits only

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gRNA NIA Null

Plasmid

#198728

Purpose

Expresses two gRNAs targeting Arabidopsis NIA1 gene for knockout. Neither gRNA is fused to a mobility signal and are therefore non-mobile.

Depositor

Friedrich Kragler

Article

Yang et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

AtNIA1 gRNAs

Expression

Plant

Promoter

pU6-26, pU6-29

Available Since

May 12, 2023

Availability

Academic Institutions and Nonprofits only

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TrpLE-M-nMSP1E3D1

Plasmid

#179949

Purpose

Tagless membrane scaffold protein (MSP) for formation of nanodiscs.

Depositor

Kerney Glover

Article

Julien et al Anal Biochem. 2021 Nov 27:114497. doi: 1

Insert

TrpLE-M-nMSP1E3D1

Tags

TrpLE

Expression

Bacterial

Mutation

Native Trp residues have been mutated to Phe resi…

Promoter

T7

Available Since

Feb. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pBabe-PI(WT)-Src(YF)-mCherry

Plasmid

#87357

Purpose

PI(WT): Photo-inhibitable/blue light sensitive; YF: Y535F const. active mutation

Depositor

Klaus Hahn

Article

Dagliyan et al Science. 2016 Dec 16;354(6318):1441-1444

Inserts

LOV2
Src isoform 2 (Src Mouse)

Tags

mCherry

Expression

Bacterial and Mammalian

Mutation

Y535F

Promoter

CMV

Available Since

April 27, 2017

Availability

Academic Institutions and Nonprofits only

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pBabe-PI(WT)-Src(KD)-mCherry

Plasmid

#87358

Purpose

PI(WT): Photo-inhibitable/blue light sensitive; kinase dead Src mutation

Depositor

Klaus Hahn

Article

Dagliyan et al Science. 2016 Dec 16;354(6318):1441-1444

Inserts

LOV2
Src (Src Mouse)

Tags

mCherry

Expression

Bacterial and Mammalian

Mutation

D388R

Promoter

CMV

Available Since

April 27, 2017

Availability

Academic Institutions and Nonprofits only

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pUSE-Src-KD-UniRapR-mCerulean-myc

Plasmid

#45382

Depositor

Klaus Hahn

Article

Dagliyan et al Proc Natl Acad Sci U S A. 2013 Apr 23;11

Insert

Src-KD-UniRapR-mCerulean-myc

Tags

mCerulean and myc

Expression

Mammalian

Mutation

mSrc R388D (kinase dead), Y529F

Promoter

CMV

Available Since

Aug. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pBabe-Tet-off-puro-mCherry-PA(wt)-Vav2

Plasmid

#91873

Purpose

Photo-activatable/light sensitive Vav2

Depositor

Klaus Hahn

Article

Dagliyan et al Science. 2016 Dec 16;354(6318):1441-1444

Inserts

LOV2
VAV2 DH/PH/ZF (Vav2 Mouse)

Tags

mCherry

Expression

Bacterial and Mammalian

Available Since

July 14, 2017

Availability

Academic Institutions and Nonprofits only

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Tol2 Krt4 Src (YF)-uniRapR-cerulean

Plasmid

#45509

Depositor

Klaus Hahn, Anna Huttenlocher

Article

Dagliyan et al Proc Natl Acad Sci U S A. 2013 Apr 23;11

Insert

Src (YF)-uniRapR-cerulean

Use

Zebrafish expression

Tags

cerulean

Mutation

Y732F

Promoter

krt4

Available Since

Oct. 29, 2013

Availability

Academic Institutions and Nonprofits only

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pBabe-Tet-off-uniRapR-mCherry-Vav2(DPZ)

Plasmid

#91874

Purpose

Rapamycin-activatable Vav2;DPZ: DH-PH-ZnF domains of Vav2

Depositor

Klaus Hahn

Article

Dagliyan et al Science. 2016 Dec 16;354(6318):1441-1444

Insert

Rapamycin-activatable Vav2;DPZ: DH-PH-ZnF domains of Vav2 (VAV2 Human)

Tags

mCherry

Expression

Bacterial and Mammalian

Available Since

Aug. 28, 2017

Availability

Academic Institutions and Nonprofits only

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gRNA NIA TLS2

Plasmid

#196981

Purpose

Expresses two gRNAs targeting Arabidopsis NIA1 gene for knockout. Each gRNA is fused to TLS2 mobility signal that confers graft mobility.

Depositor

Friedrich Kragler

Article

Yang et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

AtNIA1 gRNAs fused to TLS2 tags

Expression

Plant

Promoter

pU6-26, pU6-29

Available Since

May 12, 2023

Availability

Academic Institutions and Nonprofits only

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gRNA NIA TLS1

Plasmid

#196980

Purpose

Expresses two gRNAs targeting Arabidopsis NIA1 gene for knockout. Each gRNA is fused to TLS1 mobility signal that confers graft mobility.

Depositor

Friedrich Kragler

Article

Yang et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

AtNIA1 gRNAs fused to TLS1 tags

Expression

Plant

Promoter

pU6-26, pU6-29

Available Since

May 12, 2023

Availability

Academic Institutions and Nonprofits only

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TRBV18

Plasmid

#237942

Purpose

Encodes TRBV18 allele and TRAC to generate TCRs via cloning

Depositor

Michael Birnbaum

Article

Gaglione et al bioRxiv 2025.04.24.649726

Insert

TRBV18-TRAC (TRBV18 Human)

Use

Synthetic Biology

Mutation

None

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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pC2.4

Plasmid

#226557

Purpose

NATURA gene encoding plasmid

Depositor

Dave Wee

Article

Tabaglio et al Mol Ther Nucleic Acids. 2025 Jan 7;36(1)

Insert

NATURA reporter gene

Use

Luciferase; Piggybac

Tags

EGFP and Flag

Expression

Mammalian

Promoter

EF1alpha

Available Since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

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TRAV25

Plasmid

#237982

Purpose

Encodes TRAV25 allele, P2A, and TRBC to generate TCRs via cloning

Depositor

Michael Birnbaum

Article

Gaglione et al bioRxiv 2025.04.24.649726

Insert

TRBC-P2A-TRAV25 (TRAV25 Human)

Use

Synthetic Biology

Mutation

None

Available Since

July 17, 2025

Availability

Academic Institutions and Nonprofits only

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TRBV6-4

Plasmid

#237916

Purpose

Encodes TRBV6-4 allele and TRAC to generate TCRs via cloning

Depositor

Michael Birnbaum

Article

Gaglione et al bioRxiv 2025.04.24.649726

Insert

TRBV6-4-TRAC (TRBV6-4 Human)

Use

Synthetic Biology

Mutation

None

Available Since

July 17, 2025

Availability

Academic Institutions and Nonprofits only

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TRBV27

Plasmid

#237947

Purpose

Encodes TRBV27 allele and TRAC to generate TCRs via cloning

Depositor

Michael Birnbaum

Article

Gaglione et al bioRxiv 2025.04.24.649726

Insert

TRBV27-TRAC (TRBV27 Human)

Use

Synthetic Biology

Mutation

None

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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TRAV19

Plasmid

#237976

Purpose

Encodes TRAV19 allele, P2A, and TRBC to generate TCRs via cloning

Depositor

Michael Birnbaum

Article

Gaglione et al bioRxiv 2025.04.24.649726

Insert

TRBC-P2A-TRAV19 (TRAV19 Human)

Use

Synthetic Biology

Mutation

None

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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TRAV12-2

Plasmid

#237968

Purpose

Encodes TRAV12-2 allele, P2A, and TRBC to generate TCRs via cloning

Depositor

Michael Birnbaum

Article

Gaglione et al bioRxiv 2025.04.24.649726

Insert

TRBC-P2A-TRAV12-2 (TRAV12-2 Human)

Use

Synthetic Biology

Mutation

None

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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