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pRRG36-ChAT

Plasmid

#99069

Purpose

dsRNA and riboprobe synthesis for Schmidtea mediterranea ChAT (choline acetyltransferase)

Depositor

Phillip Newmark

Article

Roberts-Galbraith et al Proc Natl Acad Sci U S A. 2013 Jan 22;11

Insert

ChAT (choline acetyltransferase) in situ probe

Use

T/a cloning vector for dsrna generation and the g…

Tags

Expression

Mutation

Promoter

Available since

Sept. 18, 2017

Availability

Academic Institutions and Nonprofits only

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pSAVED-CHAT_PCaspase_PCi_PC-σ

Plasmid

#214040

Purpose

Bacterial expression plasmid for SAVED-CHAT, PCaspase, PCi, and PC-σ from Haliangium ochraceum

Depositor

Raymond Staals

Article

Steens et al Science. 2024 Feb 2;383(6682):512-519. d

Insert

SAVED-CHAT-Pcaspase-Pci-PC-σ

Use

Tags

Expression

Bacterial

Mutation

WT

Promoter

lacUV5 promoter

Available since

June 14, 2024

Availability

Academic Institutions and Nonprofits only

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pCDZ2FE_Champ1_1-812

Plasmid

#105675

Purpose

Expresses Flag-tagged full-length human Champ1 protein

Depositor

Thanos Halazonetis

Insert

Champ1 (CHAMP1 Human)

Use

Tags

2X Flag tag

Expression

Mammalian

Mutation

Promoter

Available since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

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pFS_0351_pET30-RT-CRISPR_Fusicatenibacter_saccharivorans_ARRAY_1

Plasmid

#116963

Purpose

Expresses FsRT-Cas1-Cas2 under pT7lac promoter, encodes FsCRISPR array 1, compatible with SENECA acquisition readout

Depositor

Randall Platt

Article

Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

Insert

Fusicatenibacter saccharivorans RT-Cas1-Cas2

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Dec. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pFS_0353_pET30-RT-CRISPR_Fusicatenibacter_saccharivorans_ARRAY_2

Plasmid

#116965

Purpose

Expresses FsRT-Cas1-Cas2 under pT7lac promoter, encodes FsCRISPR array 2, compatible with SENECA acquisition readout

Depositor

Randall Platt

Article

Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

Insert

Fusicatenibacter saccharivorans RT-Cas1-Cas2

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Dec. 4, 2018

Availability

Academic Institutions and Nonprofits only

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RCASBP-Y CHA

Plasmid

#11480

Depositor

William Pavan

Article

Loftus et al DNA Res. 2001 Oct 31. 8(5):221-6.

Insert

Gateway ccdB reading frame cassette

Use

Retroviral

Tags

HA

Expression

Mutation

Promoter

Available since

March 22, 2006

Availability

Academic Institutions and Nonprofits only

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pFS_0352_pET30-RT-CRISPR_Fusicatenibacter_saccharivorans_ARRAY_1_RC

Plasmid

#116964

Purpose

Expresses FsRT-Cas1-Cas2 under pT7lac promoter, encodes FsCRISPR array 1 RC, compatible with SENECA acquisition readout

Depositor

Randall Platt

Article

Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

Insert

Fusicatenibacter saccharivorans RT-Cas1-Cas2

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pMH-HT_CtCHAD

Plasmid

#127785

Purpose

Expresses CtCHAD in E. coli cells

Depositor

Michael Hothorn

Article

Lorenzo-Orts et al Life Sci Alliance. 2019 May 27;2(3). pii

Insert

CtCHAD (CT0884 Chlorobium tepidum)

Use

Tags

6xHis tag + TEV cleavage site

Expression

Bacterial

Mutation

Residues 208-522 (CHAD domain)

Promoter

Available since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pCHA1.1-Rap2a-V5

Plasmid

#149509

Purpose

Stable expression of human Rap2a.

Depositor

David Sabatini

Article

Adelmann et al Nature. 2020 Nov 18. pii: 10.1038/s41586

Insert

RAP2 (RAP2A Human)

Use

Lentiviral

Tags

V5

Expression

Mutation

Codon Optimized Sequence

Promoter

Ubc

Available since

Nov. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pMH-HT_SsCHAD

Plasmid

#127783

Purpose

Expresses SsCHAD in E. coli cells

Depositor

Michael Hothorn

Article

Lorenzo-Orts et al Life Sci Alliance. 2019 May 27;2(3). pii

Insert

SsCHAD (SULM_RS11045 Sulfolobus solfataricus)

Use

Tags

6xHis tag + TEV cleavage site

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pFS_0354_pET30-RT-CRISPR_Fusicatenibacter_saccharivorans_ARRAY_2_RC

Plasmid

#116966

Purpose

Expresses FsRT-Cas1-Cas2 under pT7lac promoter, encodes FsCRISPR array 2 RC, compatible with SENECA acquisition readout

Depositor

Randall Platt

Article

Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

Insert

Fusicatenibacter saccharivorans RT-Cas1-Cas2

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pAW8ENdeI-cHAIAA17

Plasmid

#48990

Purpose

for Cre-lox cassette exchange of C-terminal HA-auxin-inducible degron tagged gene sequences into the S. pombe urg1 locus

Depositor

Tony Carr

Article

Watson et al PLoS One. 2013 Dec 20;8(12):e83800. doi:

Type

Empty backbone

Use

Cre/Lox

Tags

IAA17 degron tag

Expression

Mutation

Promoter

Available since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pH7m34GW_RcCHAD-mCherry

Plasmid

#127784

Purpose

RcCHAD-mCherry expression in plants

Depositor

Michael Hothorn

Article

Lorenzo-Orts et al Life Sci Alliance. 2019 May 27;2(3). pii

Insert

Ubi10p:RcCHAD-mCherry

Use

Tags

mCherry

Expression

Plant

Mutation

Promoter

Ubiquitin10 promoter + 5' UTR

Available since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pMH-HT_RcCHAD

Plasmid

#127782

Purpose

Expresses RcCHAD in E. coli cells

Depositor

Michael Hothorn

Article

Lorenzo-Orts et al Life Sci Alliance. 2019 May 27;2(3). pii

Insert

RcCHAD

Use

Tags

6xHis tag + TEV cleavage site

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pExchange_tdk_BT1311p-tetR-3

Plasmid

#90327

Purpose

Suicide vector for integration of a constitutively expressed TetR by promoter BT1311 at B. thetatiotaomicron genomic position 6193956 and 6193957

Depositor

Andrew Goodman

Article

Lim et al Cell. 2017 Apr 20;169(3):547-558.e15. do

Insert

TetR

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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pET28b-pchA WT

Plasmid

#87102

Purpose

expresses PchA WT, the isochorismate synthase from Pseudomonas aeruginosa

Depositor

Audrey Lamb

Article

Meneely et al Arch Biochem Biophys. 2013 Oct 1;538(1):

Insert

pchA WT

Use

Tags

his-tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 15, 2017

Availability

Academic Institutions and Nonprofits only

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pHRSV_CHARMS-3xAla

Plasmid

#233921

Purpose

Mammalian expression of Chimeric Higher-Order Assemblies for Receptor Mediated Signaling (CHARMS) variant: CHARMS-3xAla (mutated TRAF6 binding site)

Depositor

Marcus Taylor

Article

Lichtenstein et al bioRxiv 2024.05.08.593169

Insert

CHARMS-3xAla

Use

Lentiviral

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

SV40

Available since

April 28, 2025

Availability

Academic Institutions and Nonprofits only

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pHRSV_CHARMS-DHF

Plasmid

#233922

Purpose

Mammalian expression of Chimeric Higher-Order Assemblies for Receptor Mediated Signaling (CHARMS) variant: CHARMS-DHF (Designed Helical Filament)

Depositor

Marcus Taylor

Article

Lichtenstein et al bioRxiv 2024.05.08.593169

Insert

CHARMS-DHF

Use

Lentiviral

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

SV40

Available since

April 28, 2025

Availability

Academic Institutions and Nonprofits only

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pHRSV_CHARMS-1xA

Plasmid

#233924

Purpose

Mammalian expression of Chimeric Higher-Order Assemblies for Receptor Mediated Signaling (CHARMS) variant: CHARMS-1xA which contains 1 TRAF6 binding motif with a alanine mutation

Depositor

Marcus Taylor

Article

Lichtenstein et al bioRxiv 2024.05.08.593169

Insert

CHARMS-1xA

Use

Lentiviral

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

SV40

Available since

April 28, 2025

Availability

Academic Institutions and Nonprofits only

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pHRSV_CHARMS-3xA

Plasmid

#233926

Purpose

Mammalian expression of Chimeric Higher-Order Assemblies for Receptor Mediated Signaling (CHARMS) variant: CHARMS-3xA which contains 3 TRAF6 binding motif with a alanine mutation

Depositor

Marcus Taylor

Article

Lichtenstein et al bioRxiv 2024.05.08.593169

Insert

CHARMS-3xA

Use

Lentiviral

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

SV40

Available since

April 28, 2025

Availability

Academic Institutions and Nonprofits only

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