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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 1,118 results for: gibson assembly

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Showing: 61 - 80 of 1118 results

  1. pLFYalpha

    Plasmid
    #243804
    Purpose
    Dropout vector for genomic integration into yeast strain LFYalpha. GFP is flanked by BamHI sites for easy cloning using Gibson assembly. Construct contains attB site for the serine recombinase BxB1.
    Depositor
    Sai Reddy
    Publication
    Lester et al bioRxiv
    Type
    Empty backbone
    Use
    Synthetic Biology
    Available since
    Oct. 8, 2025
    Availability
    Academic Institutions and Nonprofits only
    243804-plasmid-map-sequence-id-487916

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  2. PMXS-GOT1

    Plasmid
    #72872
    Purpose
    The retroviral GOT1 vector was generated by cloning an sgRNA resistant human GOT1 gene block into the pMXS-ires-blast vector by Gibson Assembly.
    Depositor
    David Sabatini
    Publication
    Birsoy et al Cell. 2015 Jul 30;162(3):540-51. doi: 10
    Insert
    GOT1 (GOT1 Human)
    Use
    Retroviral
    Expression
    Mammalian
    Mutation
    sgRNA resistant human GOT1, 7 silent mutations c1…
    Available since
    Feb. 2, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles

  4. pYZ033

    Plasmid
    #98404
    Purpose
    Entry vector for S. pombe CRISPR-Cas9 system. The gRNA can be integrated easily through Gibson Assembly with the plasmid backbone digested by Not1
    Depositor
    Jef Boeke
    Publication
    Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.
    Type
    Empty backbone
    Use
    CRISPR and Synthetic Biology
    Available since
    Feb. 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    98404-plasmid-map-sequence-id-198860

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  12. p006-GFP-pBAD

    Plasmid
    #108315
    Purpose
    GFP from jellyfish Aquorea sp. under pBAD promoter with AraC repressor; kanamycin resistant.
    Depositor
    Jaroslaw Bryk
    Publication
    Siddall et al bioRxiv 2021.06.20.449138
    Insert
    GFP under control of arabinose-inducible promoter pBAD
    Expression
    Bacterial
    Promoter
    pBAD
    Available since
    Aug. 17, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    108315-plasmid-map-sequence-id-218821

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  19. pUCC001

    Plasmid
    #124451
    Purpose
    Multi-species CRISPR/Cas9 coexpression system, optimized for Golden Gate cloning of gRNA targets
    Depositor
    John Morrissey
    Publication
    Rajkumar et al Front Bioeng Biotechnol. 2019 May 7;7:97
    Insert
    HH-BsaI
    Expression
    Yeast
    Mutation
    gRNA expression cassette from pUDP002 modified to…
    Promoter
    TDH3 promoter from S.cerevisiae (already present …
    Available since
    Sept. 26, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    124451-plasmid-map-sequence-id-257763

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Showing: 61 - 80 of 1118 results