Addgene

Showing: 61 - 80 of 6508 results

Results per page:

Sort results by:

pHR-human U6-sgRNA-EF1Alpha-puro-T2A-BFP

Plasmid

#118594

Purpose

Parental vector for the CRISPRa libraries. Expresses an sgRNA from the human U6 promoter and a puromycin resistance cassette and BFP from the EF1Alpha promoter

Depositor

Stanley Qi

Article

Liu et al Cell Stem Cell. 2018 Sep 28. pii: S1934-

Insert

sgRNA/Puro-T2A-BFP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1Alpha

Available since

Jan. 25, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
Pet28a_Synthetic_Human_H2A.1

Plasmid

#42634

Depositor

Joe Landry

Insert

Human H2A.1 (H2AC13 Synthetic, Human)

Use

Tags

Untagged

Expression

Bacterial

Mutation

Codon Optimized

Promoter

T7

Available since

Feb. 25, 2013

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pcDNA3.1 human cofilin WT

Plasmid

#50853

Purpose

expresses human cofilin in mammalian cells

Depositor

James Bamburg

Article

Garvalov et al J Neurosci. 2007 Nov 28;27(48):13117-29.

Insert

cofilin 1 (CFL1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 5, 2014

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
human oct4-GFP

Plasmid

#21153

Depositor

Rudolf Jaenisch

Article

Green et al Nano Lett. 2008 Oct . 8(10):3126-30.

Insert

hOCT4-GFP (POU5F1 Human)

Use

Human targeting

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 13, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1 human A3C

Plasmid

#105047

Purpose

Overexpression of APOBEC3C in mammalian cell lines

Depositor

Carsten Münk

Article

Jaguva Vasudevan et al J Mol Biol. 2017 Apr 21;429(8):1171-1191

Insert

APOBEC3C (A3C) (APOBEC3C Human)

Use

Tags

HA tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 8, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
Human USP30 (64-502, construct 2, MG-26-18)

Plasmid

#110744

Purpose

Bacterial expression of human USP30 (construct 2)

Depositor

David Komander

Article

Gersch et al Nat Struct Mol Biol. 2017 Sep 25. doi: 1

Insert

USP30 (USP30 Human)

Use

Tags

N-His6-3C-GST-3C

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 14, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1 human A3C.S61P

Plasmid

#105048

Purpose

Overexpression of APOBEC3C S61P mutant in mammalian cell lines

Depositor

Carsten Münk

Article

Jaguva Vasudevan et al J Mol Biol. 2017 Apr 21;429(8):1171-1191

Insert

APOBEC3C (A3C) (APOBEC3C Human)

Use

Tags

HA tag

Expression

Mammalian

Mutation

serine 61 to proline

Promoter

CMV

Available since

Jan. 8, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1 human A3C.D99E

Plasmid

#105049

Purpose

Overexpression of APOBEC3C D99E mutant in mammalian cell lines

Depositor

Carsten Münk

Article

Jaguva Vasudevan et al J Mol Biol. 2017 Apr 21;429(8):1171-1191

Insert

APOBEC3C (A3C) (APOBEC3C Human)

Use

Tags

HA tag

Expression

Mammalian

Mutation

Aspartate 99 to glutamate

Promoter

CMV

Available since

Jan. 8, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1 human A3C.DS-EP

Plasmid

#105050

Purpose

Overexpression of APOBEC3C DS-EP mutant in mammalian cell lines

Depositor

Carsten Münk

Article

Jaguva Vasudevan et al J Mol Biol. 2017 Apr 21;429(8):1171-1191

Insert

APOBEC3C (A3C) (APOBEC3C Human)

Use

Tags

HA tag

Expression

Mammalian

Mutation

S61P, D99E

Promoter

CMV

Available since

Jan. 8, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1 human A3C.C97S

Plasmid

#105051

Purpose

Overexpression of APOBEC3C C97S mutant in mammalian cell lines

Depositor

Carsten Münk

Article

Jaguva Vasudevan et al J Mol Biol. 2017 Apr 21;429(8):1171-1191

Insert

APOBEC3C (A3C) (APOBEC3C Human)

Use

Tags

HA tag

Expression

Mammalian

Mutation

cysteine 97 to serine

Promoter

CMV

Available since

Jan. 8, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP-N1 human cofilin S3A

Plasmid

#50860

Purpose

expresses constituvely active cofilin fused to EGFP in mammalian cells

Depositor

James Bamburg

Article

Garvalov et al J Neurosci. 2007 Nov 28;27(48):13117-29.

Insert

cofilin 1 S3A (CFL1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

S3A constitutively active

Promoter

CMV

Available since

Feb. 5, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP-N1 human cofilin S3E

Plasmid

#50861

Purpose

expresses psuedo-phosporylated, non-activatable cofilin fused to EGFP in mammalian cells

Depositor

James Bamburg

Article

Garvalov et al J Neurosci. 2007 Nov 28;27(48):13117-29.

Insert

cofilin 1 S3E (CFL1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

S3E psuedo-phosporylated, non-activatable

Promoter

CMV

Available since

Feb. 5, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
HOXC13(human) GSTfusion-pET

Plasmid

#8680

Depositor

Corey Largman

Article

Komuves et al Dev Dyn 2003 Jun;227(2):192-202.

Insert

HOXC13 (HOXC13 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

makes protein by Coomassie blue staining

Promoter

Available since

May 30, 2006

Availability

Academic Institutions and Nonprofits only
HOXC4 (human) MSCV

Plasmid

#8546

Depositor

Corey Largman

Article

Shen et al Nucleic Acids Res 1996 Mar 1;24(5):898-9

Insert

HOXC4 (HOXC4 Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Contains improved ribosomal binding site and NcoI…

Promoter

Available since

May 30, 2006

Availability

Academic Institutions and Nonprofits only
pmRFP-N1 human cofilin S3A

Plasmid

#50857

Purpose

expresses constituvely active cofilin fused to mRFP in mammalian cells

Depositor

James Bamburg

Article

Garvalov et al J Neurosci. 2007 Nov 28;27(48):13117-29.

Insert

cofilin 1 S3A (CFL1 Human)

Use

Tags

mRFP

Expression

Mammalian

Mutation

S3A constitutively active

Promoter

CMV

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only
pmRFP-N1 human cofilin S3E

Plasmid

#50858

Purpose

expresses psuedo-phosporylated, non-activatable cofilin fused to mRFP in mammalian cells

Depositor

James Bamburg

Article

Garvalov et al J Neurosci. 2007 Nov 28;27(48):13117-29.

Insert

cofilin 1 S3E (CFL1 Human)

Use

Tags

mRFP

Expression

Mammalian

Mutation

S3E psuedo-phosporylated, non-activatable

Promoter

CMV

Available since

Feb. 20, 2014

Availability

Academic Institutions and Nonprofits only
HOXB6 (human) FLAG pCMV

Plasmid

#8815

Depositor

Corey Largman

Article

Shen et al Proc Natl Acad Sci U S A 1989 Nov;86(21)

Insert

HOXB6 (HOXB6 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

contains improved ribosomal binding site and FLAG…

Promoter

Available since

May 30, 2006

Availability

Academic Institutions and Nonprofits only
Human USP30 (64-502, construct 8, MG-26-21)

Plasmid

#110745

Purpose

Bacterial expression of human USP30 (construct 8)

Depositor

David Komander

Article

Gersch et al Nat Struct Mol Biol. 2017 Sep 25. doi: 1

Insert

USP30 (USP30 Human)

Use

Tags

N-His6-3C-GST-3C

Expression

Bacterial

Mutation

F348D, M350D, I353E + insertion deletion: 64-357;…

Promoter

T7

Available since

June 14, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
Human USP30 (64-502, construct 13, MG-26-82)

Plasmid

#110746

Purpose

Bacterial expression of human USP30 (construct 13)

Depositor

David Komander

Article

Gersch et al Nat Struct Mol Biol. 2017 Sep 25. doi: 1

Insert

USP30 (USP30 Human)

Use

Tags

N-His6-3C-GST-3C

Expression

Bacterial

Mutation

F348D, M350S, I353E + insertion deletion: 64-178;…

Promoter

T7

Available since

June 14, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pClneoFLAG human Dendrin

Plasmid

#47934

Purpose

expresses FLAG tagged human Dendrin in mammalian cells

Depositor

Yutaka Hata

Article

Kawata et al J Biochem. 2006 May;139(5):931-9.

Insert

Dendrin-1 (DDN Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

contains amino acids 2-711 of human Dendrin

Promoter

CMV

Available since

Sept. 9, 2013

Availability

Academic Institutions and Nonprofits only