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Showing: 61 - 80 of 128 results

  7. pHR-SIN-BX SpyCatcher003-(short)mCD80

    Plasmid
    #210567
    Purpose
    Expresses Spycatcher003 on the cell surface using mouse CD80 (short) hinge, transmembrane, and cytoplasmic domain
    Depositor
    Omer Dushek
    Article
    Patel et al EMBO J. 2024 Jan;43(1):132-150. doi: 10.
    Insert
    Spycatcher003 fused to mouse CD80 (short hinge + transmembrane + cytoplasmic domain)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    SFFV
    Available since
    Dec. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    210567-plasmid-map-sequence-id-417655

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  8. TfR-sfGFP-myc tag-SpyCatcher003

    Plasmid
    #133451
    Purpose
    Expresses SpyCatcher003 for display at the cell surface of mammalian cells, with superfolder GFP for visualization and myc tag for antibody detection
    Depositor
    Mark Howarth
    Article
    Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p
    Insert
    TfR-sfGFP-myc tag-SpyCatcher003
    Use
    Tags
    GSSGS and myc tag
    Expression
    Mammalian
    Mutation
    Contains C20 and A23 mutations that improve plasm…
    Promoter
    CMV
    Available since
    Dec. 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    133451-plasmid-map-sequence-id-267048

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  16. TfR-SpyCatcher003(K31TAG)-sfGFP-MycTag-CTag

    Plasmid
    #210021
    Purpose
    Expression of transferrin receptor transmembrane domain and photocaged SpyCatcher003(K31HCK)-sfGFP on mammalian cell surface.
    Depositor
    Vesa Hytönen
    Article
    Rahikainen et al J Am Chem Soc. 2023 Nov 15;145(45):24459
    Insert
    Transferrin receptor transmembrane domain-SpyCatcher003(K31TAG)-superfolderGFP-MycTag-CTag (TFRC Human, Synthetic)
    Use
    Tags
    MycTag, CTag
    Expression
    Mammalian
    Mutation
    Transferrin receptor transmembrane domain with Y2…
    Promoter
    CMV promoter
    Available since
    March 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    210021-plasmid-map-sequence-id-425593

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  18. pQE80l B-CC-B

    Plasmid
    #196479
    Purpose
    Protein expression of SpyCatcher-ELP-CarHCC-ELP-SpyCatcher, with TEV cleavage site and MMP cleavage site respectively before and after each SpyCatcher, and GRGD site linked with (GGGGS)2 before CarHCC
    Depositor
    Fei Sun
    Insert
    B-CC-B
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    196479-plasmid-map-sequence-id-387244

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  19. pQE80l BCB

    Plasmid
    #196472
    Purpose
    Protein expression of SpyCatcher-ELP-CarHC-ELP-SpyCatcher, with TEV cleavage site and MMP cleavage site respectively before and after each SpyCatcher
    Depositor
    Fei Sun
    Article
    Wang et al Proc Natl Acad Sci U S A. 2017 Jun 6;114
    Insert
    BCB
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    196472-plasmid-map-sequence-id-387266

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  20. pQE80l B-CN-B

    Plasmid
    #196477
    Purpose
    Protein expression of SpyCatcher-ELP-CarHCN-ELP-SpyCatcher, with TEV cleavage site and MMP cleavage site respectively before and after each SpyCatcher, and GRGD site linked with (GGGGS)2 before CarHCN
    Depositor
    Fei Sun
    Article
    Yang et al Sci Adv. 2022 Apr;8(13):eabm5482. doi: 1
    Insert
    B-CN-B
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    196477-plasmid-map-sequence-id-387241

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Showing: 61 - 80 of 128 results