We narrowed to 134 results for: spycatcher
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Plasmid#133566PurposeExpresses EGFP-Talin head-SpyCatcher003 in mammalian cellsDepositorInsertEGFP-Talin head-SpyCatcher003
ExpressionMammalianPromoterCMVAvailable SinceDec. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)_SpyCatcher-6aa-sfCherry2(1-10)
Plasmid#117484PurposeExpresses tandem binder (SpyCatcher002-6aa-sfCherry2(1-10)) in mammalian cellsDepositorInsertSpyCatcher002-6aa-sfCherry2(1-10)
ExpressionMammalianPromoterCMVAvailable SinceJuly 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHR-SIN-BX SpyCatcher003-hCD52
Plasmid#210565PurposeExpresses Spycatcher003 on the cell surface using human CD52 hingeDepositorInsertSpycatcher003 fused to human CD52 hinge
UseLentiviralExpressionMammalianPromoterSFFVAvailable SinceDec. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDEST14-SpyCatcher003-TEVs-SpyTag003DA
Plasmid#216283PurposeExpresses SpyCatcher003-TEV site-SpyTag003DA in bacterial cytoplasm, to allow TEV protease to remove SpyTag003DA and thereby uncage SpyCatcher003 reactivity.DepositorInsertSpyCatcher003-TEVs-SpyTag003DA
TagsHis6 and TEV cleavage siteExpressionBacterialPromoterT7 promoterAvailable SinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET22b 6His SpyCatcher-GRFT
Plasmid#204502PurposeBacterial expression of GRFT with N-terminal SpyCatcher fusion and GS linkerDepositorInsertGRFT
UseSynthetic BiologyTags6xHis and SpyCatcherExpressionBacterialPromoterT7Available SinceSept. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-P3-IgKL-SpyCatcher003-GINKO2
Plasmid#234544Purposeto express a potassium sensor tagged with SpyCatcher in the liverDepositorInsertIgKL-SpyCatcher003-GINKO2
UseAAVMutationNonePromoterP3Available SinceMay 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-P3-IgKL-SpyCatcher003-mNeonGreen
Plasmid#234543Purposeto express a green fluorescent protein tagged with SpyCatcher in the liverDepositorInsertIgKL-SpyCatcher003-mNeonGreen
UseAAVMutationNonePromoterP3Available SinceApril 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-P3-IgKL-SpyCatcher003-SEP
Plasmid#234541Purposeto express a pH sensor tagged with SpyCatcher in the liverDepositorInsertIgKL-SpyCatcher003-SEP
UseAAVMutationNonePromoterP3Available SinceApril 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET23a-His-TEV-SpyCatcher3
Plasmid#235149PurposeBacterial expression of His-tagged SpyCatcher3 separated by a TEV protease recognition site.DepositorInsertSpyCatcher3
TagsHisExpressionBacterialPromoterT7Available SinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pDEST14-Cys-SpyCatcher (Min)
Plasmid#216713PurposeExpresses truncated minimal form of SpyCatcher with free cysteine for labeling in bacteria. See also Li...Howarth, J Mol. Biol., 2014DepositorInsertCys-SpyCatcher (Min)
Tags6xHisExpressionBacterialMutationSee Li...Howarth, J. Mol. Biol., 2014Available SinceApril 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
CMVTO-p450-hcvs-SpyCatcher
Plasmid#179677PurposeHCVP-cleavable ER-associated SpyCatcherDepositorInsertHCVP SpyCatcher
ExpressionMammalianAvailable SinceMarch 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
TfR-sfGFP-myc tag-SpyCatcher003
Plasmid#133451PurposeExpresses SpyCatcher003 for display at the cell surface of mammalian cells, with superfolder GFP for visualization and myc tag for antibody detectionDepositorInsertTfR-sfGFP-myc tag-SpyCatcher003
TagsGSSGS and myc tagExpressionMammalianMutationContains C20 and A23 mutations that improve plasm…PromoterCMVAvailable SinceDec. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHR-SIN-BX SpyCatcher003-(short)mCD80
Plasmid#210567PurposeExpresses Spycatcher003 on the cell surface using mouse CD80 (short) hinge, transmembrane, and cytoplasmic domainDepositorInsertSpycatcher003 fused to mouse CD80 (short hinge + transmembrane + cytoplasmic domain)
UseLentiviralExpressionMammalianPromoterSFFVAvailable SinceDec. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
His6-SUMO-SPMc-SpyCatcher003-Ctag
Plasmid#246648PurposeExpresses His6-SUMO-SPMc-SpyCatcher003-Ctag in bacterial cells for calcium-induced NeissLock protein ligationDepositorInsertHis6-SUMO-SPMc-SpyCatcher003-Ctag
TagsCtag, His-tag, SUMO, and SpyCatcher003ExpressionBacterialMutationThe SPMc comprises the C-terminal fragment of th…PromoterT7Available SinceOct. 31, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-DIO-ECS-dSpyCatcher003
Plasmid#234963PurposeCre-dependent ECS-facing destabilized SpyCatcher003 (facing the extracellular space)DepositorInsertECS-dSC
UseAAV and Cre/LoxExpressionMammalianMutationNoneAvailable SinceMay 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-mGFAP(ABC1D)-ECS-SpyCatcher003
Plasmid#234545Purposeto express SpyCatcher on the extracellular side of astrocytic plasmamembraneDepositorInsertECS-SC
UseAAVMutationNonePromotermGFAP(ABC1D)Available SinceMay 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-mGFAP(ABC1D)-ECS-dSpyCatcher003
Plasmid#234546Purposeto express dSpyCatcher on the extracellular side of astrocytic plasmamembraneDepositorInsertECS-dSC
UseAAVMutationNonePromotermGFAP(ABC1D)Available SinceMay 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
SpyCatcher003(K31TAG)-Talin rod-mScarletI
Plasmid#210022PurposeExpression of SpyCatcher003(K31TAG)-Talin rod(434-2541)-mScarletI in mammalian cellsDepositorInsertSpyCatcher003(K31TAG)-Talin rod(434-2541)-mScarletI
TagsC-terminal mScarletIExpressionMammalianPromoterCMVAvailable SinceDec. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
EGFP-Talin head-SpyCatcher003(K31TAG)
Plasmid#210023PurposeExpression of EGFP-Talin head(1-433)-SpyCatcher003(K31TAG) in mammalian cellsDepositorInsertEGFP-Talin head (1-433)-SpyCatcher003(K31TAG)
ExpressionMammalianMutationSpyCatcher003 with K31TAG (Amber stop codon) muta…PromoterCMVAvailable SinceDec. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET23a-His-TEV-HaloTag-linker-SpyCatcher3
Plasmid#235150PurposeBacterial expression of His-tagged HaloTag-linker-SpyCatcher3 separated by a TEV protease recognition site.DepositorInsertHaloTag linked to SpyCatcher3
TagsHisExpressionBacterialPromoterT7Available SinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only