We narrowed to 223 results for: stem loop

1. pSG13-pBAD-TetRssrA

   Plasmid

   #102449

   Purpose

   Expresses TetR protein with ssrA degradation tag. The expression is controlled by a pBAD promoter.

   Depositor

   Richard Murray

   Article

   Guo et al ACS Synth Biol. 2019 Mar 15;8(3):606-610

   Insert

   TetR (tetR )
   

   Tags

   ssrA

   Expression

   Bacterial

   Promoter

   pBAD

   Available Since

   Oct. 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSG25-pBAD-tetO-deGFPssrA

   Plasmid

   #102452

   Purpose

   Expresses deGFP fluorescent protein with ssrA degradation tag in E. coli. The expression is controlled by a pBAD-tetO combinatorial promoter.

   Depositor

   Richard Murray

   Article

   Guo et al ACS Synth Biol. 2019 Mar 15;8(3):606-610

   Insert

   deGFP
   

   Tags

   ssrA

   Expression

   Bacterial

   Promoter

   pBAD-tetO

   Available Since

   Oct. 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSG81-pLas-tetO-deGFPssrA

   Plasmid

   #102454

   Purpose

   Expresses deGFP fluorescent protein with ssrA degradation tag in E. coli. The expression is controlled by a pLas-tetO combinatorial promoter.

   Depositor

   Richard Murray

   Article

   Guo et al ACS Synth Biol. 2019 Mar 15;8(3):606-610

   Insert

   deGFP
   

   Tags

   ssrA

   Expression

   Bacterial

   Promoter

   pLas-tetO

   Available Since

   Oct. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRP-iSL(MCS1+2)

   Plasmid

   #69245

   Purpose

   Tet-inducible RRNA promoter driven plasmid for the generation of stem loop RNAi transcripts from a random RRNA spacer locus in T. brucei.

   Depositor

   David Horn

   Type

   Empty backbone

   Use

   Expression in trypanosoma brucei

   Promoter

   RRNA promoter

   Available Since

   Oct. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

5. pET263-pUC57 24xMS2V7

   Plasmid

   #140705

   Purpose

   MS2V7 cloning

   Depositor

   Evelina Tutucci, Robert Singer

   Article

   Tutucci et al Nat Methods. 2017 Nov 13. doi: 10.1038/n

   Insert

   24xMS2V7
   

   Use

   Cloning vector

   Mutation

   24 MS2 loops V7, high-affinity C-variant, inter-s…

   Promoter

   None

   Available Since

   April 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCDNA3 ATP5O(5’UTR)-SL-FF

   Plasmid

   #85491

   Purpose

   Firefly luciferase under the control of ATP5O 5'UTR followed by a stem-loop

   Depositor

   Ivan Topisirovic

   Article

   Gandin et al Genome Res. 2016 May;26(5):636-48. doi:

   Insert

   5'UTR of ATP5O followed by stem loop (ATP5PO Human)
   

   Use

   Luciferase

   Tags

   Firefly luciferase

   Expression

   Mammalian

   Available Since

   Jan. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1 - HA-KLF4 FL

   Plasmid

   #34593

   Depositor

   Michael Ruppert

   Article

   Lin et al Mol Cell Biol. 2011 Jun;31(12):2513-27.

   Insert

   KLF4 (KLF4 Human)
   

   Tags

   HA tag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 2, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMIR-Report-Luc-KLF4-FL

   Plasmid

   #34597

   Depositor

   Michael Ruppert

   Article

   Lin et al Mol Cell Biol. 2011 Jun;31(12):2513-27.

   Insert

   KLF4 (KLF4 Human)
   

   Use

   Luciferase

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 23, 2012

   Availability

   Academic Institutions and Nonprofits only

9. pBpuro - HA KLF4 FL

   Plasmid

   #34589

   Depositor

   Michael Ruppert

   Article

   Lin et al Mol Cell Biol. 2011 Jun;31(12):2513-27.

   Insert

   KLF4 (KLF4 Human)
   

   Use

   Retroviral

   Tags

   HA tag

   Expression

   Mammalian

   Promoter

   LTR

   Available Since

   Feb. 2, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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10. NLS-tdPCP-CIBN

    Plasmid

    #183937

    Purpose

    PCP tandem dimer binding to PP7 RNA stem loops coupled to optogenetic CIBN domain; bound by PHR upon blue light exposure

    Depositor

    Karsten Rippe

    Article

    Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

    Insert

    tdPCP-CIBN (CIB1 Synthetic, Mustard Weed)
    

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV (TATA box removed)

    Available Since

    June 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLJD-KLF4-ER

    Plasmid

    #34596

    Depositor

    Michael Ruppert

    Article

    Lin et al Mol Cell Biol. 2011 Jun;31(12):2513-27.

    Insert

    KLF4 (KLF4 Human)
    

    Use

    Retroviral

    Tags

    Myc epitope tag and estrogen receptor fusion

    Expression

    Mammalian

    Promoter

    LTR

    Available Since

    Feb. 2, 2012

    Availability

    Academic Institutions and Nonprofits only

12. pCR4-24XPP7SL

    Plasmid

    #31864

    Depositor

    Robert Singer

    Article

    Larson et al Science. 2011 Apr 22;332(6028):475-8.

    Insert

    24xPP7 Stem Loop Cassette
    

    Available Since

    Aug. 31, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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13. KL063 - pCAGGS-OsTIR1(F74G)-BpA-Frt-PGK-EM7-NeoR-bpA-Frt-Rosa26

    Plasmid

    #232929

    Purpose

    Targeting vector to introduce an OsTir1(F74G) expressing cassette at the mouse Rosa26 locus using Neomycin selection. AID2 degron system. Use with Addgene #64216 sgRNA.

    Depositor

    Danny Reinberg

    Article

    Lucero et al bioRxiv, March 19, 2025

    Insert

    OsTir1(F74G)
    

    Use

    Mouse Targeting

    Expression

    Mammalian

    Mutation

    changed Phenylalanine 74 to Glycine

    Available Since

    March 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pX330-NL-DHFR-SpCas9

    Plasmid

    #124522

    Purpose

    Expresses SpCas9 fused to DHFR domains on both the N-terminus and an internal loop in mammalian cells

    Depositor

    Amit Choudhary

    Article

    Manna et al Angew Chem Int Ed Engl. 2019 May 6;58(19

    Insert

    NL-DHFR-SpCas9
    

    Use

    AAV

    Tags

    Destabilized domain of E.coli dihydrofolate reduc…

    Expression

    Mammalian

    Promoter

    Cbh

    Available Since

    July 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pX330-NLC-DHFR-SpCas9

    Plasmid

    #124523

    Purpose

    Expresses SpCas9 fused to DHFR domains on both the N- and C- termini and an internal loop in mammalian cells

    Depositor

    Amit Choudhary

    Article

    Manna et al Angew Chem Int Ed Engl. 2019 May 6;58(19

    Insert

    NLC-DHFR-SpCas9
    

    Use

    AAV

    Tags

    Destabilized domain of E.coli dihydrofolate reduc…

    Expression

    Mammalian

    Promoter

    Cbh

    Available Since

    July 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCR4-24XMS2SL-stable

    Plasmid

    #31865

    Depositor

    Robert Singer

    Article

    Bertrand et al Mol Cell. 1998 Oct . 2(4):437-45.

    Insert

    24xMS2 Stem Loops - Stable
    

    Available Since

    Aug. 31, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJR85

    Plasmid

    #140095

    Purpose

    Lentiviral CRISPR guide vector expressing a eGFP-NT2 sgRNA with cs1 incorporated in the loop of the sgRNA constant region. BsmBI sites were removed to allow for programmed dual sgRNA library cloning.

    Depositor

    Jonathan Weissman

    Article

    Replogle et al Nat Biotechnol. 2020 Aug;38(8):954-961.

    Insert

    sgRNA with cs1 in stem loop 2 of the constant region
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    April 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pBA904

    Plasmid

    #122238

    Purpose

    Lentiviral CRISPR guide vector expressing a eGFP-NT2 sgRNA with cs1 incorporated in the loop of the sgRNA constant region.

    Depositor

    Jonathan Weissman

    Article

    Replogle et al Nat Biotechnol. 2020 Aug;38(8):954-961.

    Insert

    sgRNA with cs1 in stem loop 2 of the constant region
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    April 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3.1 - HA-KLF4 FL delta K5S

    Plasmid

    #34594

    Depositor

    Michael Ruppert

    Article

    Lin et al Mol Cell Biol. 2011 Jun;31(12):2513-27.

    Insert

    KLF4 (KLF4 Human)
    

    Tags

    HA tag

    Expression

    Mammalian

    Mutation

    deletion of K5S, corresponding to bases 1781 to 2…

    Promoter

    CMV

    Available Since

    Jan. 24, 2012

    Availability

    Academic Institutions and Nonprofits only

20. pBpuro - HA KLF4 FL delta K5S

    Plasmid

    #34592

    Depositor

    Michael Ruppert

    Article

    Lin et al Mol Cell Biol. 2011 Jun;31(12):2513-27.

    Insert

    KLF4 (KLF4 Human)
    

    Use

    Retroviral

    Tags

    HA tag

    Expression

    Mammalian

    Mutation

    deletion of K5S, corresponding to bases 1781 to 2…

    Promoter

    LTR

    Available Since

    Jan. 24, 2012

    Availability

    Academic Institutions and Nonprofits only