We narrowed to 866 results for: seph

1. pET28a_6HT-TBP6.7(T50A)

   Plasmid

   #112727

   Purpose

   Lav-evolved amino acid T50 within TBPs is important in engaging intramolecularly to steer also lab-evolved R49 into conformation compatible binding to RNA.

   Depositor

   Joseph Wedekind

   Article

   Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

   Insert

   6His-TEV-TBP6.7(T50A)
   

   Use

   Tags

   6His-TEV

   Expression

   Bacterial

   Mutation

   T50A

   Promoter

   T7

   Available since

   Aug. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28a_6HT-TBP6.7(R52A)

   Plasmid

   #112729

   Purpose

   52nd position is Arg in both wild-type U1A and TBPs, but the RNA recognition by this residue in both types of proteins is fundamentally different.

   Depositor

   Joseph Wedekind

   Article

   Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

   Insert

   6His-TEV-TBP6.7(R52A)
   

   Use

   Tags

   6His-TEV

   Expression

   Bacterial

   Mutation

   R52A

   Promoter

   T7

   Available since

   Aug. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28a_6HT-TBP6.7(delta C-term)

   Plasmid

   #112731

   Purpose

   This mutant of TBP6.7 is lacking residues 91-98 at C-terminus, and was used to test the involvement of those residues in TAR recognition.

   Depositor

   Joseph Wedekind

   Article

   Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

   Insert

   6His-TEV-TBP6.7(delta C-term)
   

   Use

   Tags

   6His-TEV

   Expression

   Bacterial

   Mutation

   delta C-term

   Promoter

   T7

   Available since

   Aug. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. LV-CMV-Cer-FL- Bmal L95E

   Plasmid

   #47374

   Purpose

   Bmal full length with cer tag used for mutation

   Depositor

   Joseph Takahashi

   Article

   Huang et al Science. 2012 Jul 13;337(6091):189-94. d

   Insert

   BMAL (Bmal1 Mouse)
   

   Use

   Tags

   Venus

   Expression

   Mammalian

   Mutation

   L95E

   Promoter

   CMV

   Available since

   Jan. 14, 2014

   Availability

   Academic Institutions and Nonprofits only

5. pCDNA3-HA/Bmal F423R/V435R/W427R

   Plasmid

   #47351

   Purpose

   PcHA-Bmal backbone used for the above mutation. Ref.Genes & Dev 17:1921-1932 2003

   Depositor

   Joseph Takahashi

   Article

   Huang et al Science. 2012 Jul 13;337(6091):189-94. d

   Insert

   BMAL (Bmal1 Mouse)
   

   Use

   Tags

   Ha

   Expression

   Mammalian

   Mutation

   F423R/V435R/W427R

   Promoter

   CMV

   Available since

   Jan. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

6. HLH Clock Pas 1-401 VenN L113E

   Plasmid

   #47356

   Purpose

   Clock fragment mutatation tagged with VenN used for BiFC

   Depositor

   Joseph Takahashi

   Article

   Huang et al Science. 2012 Jul 13;337(6091):189-94. d

   Insert

   Clock (Clock Mouse)
   

   Use

   Tags

   Venus aa 1-155

   Expression

   Mammalian

   Mutation

   aa 1-401, L113E

   Promoter

   CMV

   Available since

   Jan. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

7. HLH Clock Pas 1-401 VenN F122D

   Plasmid

   #47357

   Purpose

   Clock fragment mutatation tagged with VenN used for BiFC

   Depositor

   Joseph Takahashi

   Article

   Huang et al Science. 2012 Jul 13;337(6091):189-94. d

   Insert

   Clock (Clock Mouse)
   

   Use

   Tags

   Venus aa 1-155

   Expression

   Mammalian

   Mutation

   aa 1-401, F122D

   Promoter

   CMV

   Available since

   Jan. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

8. HLH Clock Pas 1-401 VenN W284A

   Plasmid

   #47358

   Purpose

   Clock fragment mutatation tagged with VenN used for BiFC

   Depositor

   Joseph Takahashi

   Article

   Huang et al Science. 2012 Jul 13;337(6091):189-94. d

   Insert

   Clock (Clock Mouse)
   

   Use

   Tags

   Venus aa 1-155

   Expression

   Mammalian

   Mutation

   aa 1-401, W284A

   Promoter

   CMV

   Available since

   Jan. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

9. HLH Clock Pas 1-401 VenN V315R

   Plasmid

   #47359

   Purpose

   Clock fragment mutatation tagged with VenN used for BiFC

   Depositor

   Joseph Takahashi

   Article

   Huang et al Science. 2012 Jul 13;337(6091):189-94. d

   Insert

   Clock (Clock Mouse)
   

   Use

   Tags

   Venus aa 1-155

   Expression

   Mammalian

   Mutation

   aa 1-401, V315R

   Promoter

   CMV

   Available since

   Jan. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

10. HLH Bmal Pas 1-465-VenC L115E

    Plasmid

    #47377

    Purpose

    Bmal fragment cloned with Venus tag used for mutation

    Depositor

    Joseph Takahashi

    Article

    Huang et al Science. 2012 Jul 13;337(6091):189-94. d

    Insert

    BMAL (Bmal1 Mouse)
    

    Use

    Tags

    Venus aa 156-239

    Expression

    Mammalian

    Mutation

    L115E

    Promoter

    CMV

    Available since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

11. HLH Clock Pas 1-401 VenN L57E

    Plasmid

    #47354

    Purpose

    Clock fragment mutatation tagged with VenN

    Depositor

    Joseph Takahashi

    Article

    Huang et al Science. 2012 Jul 13;337(6091):189-94. d

    Insert

    Clock (Clock Mouse)
    

    Use

    Tags

    Venus aa 1-155

    Expression

    Mammalian

    Mutation

    aa 1-401, L57E

    Promoter

    CMV

    Available since

    Sept. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

12. pMSCV-puro-Delta-ATG1-mMCL1

    Plasmid

    #45823

    Purpose

    This plasmid was made to show that the two MCL-1 bands DO NOT arise from a non-canonical translation initiation downstream of the start codon.

    Depositor

    Joseph Opferman

    Article

    Perciavalle et al Nat Cell Biol. 2012 Apr 29;14(6):575-83.

    Insert

    Delta ATG1-mMCL1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    The first amino acid (ATG) is deleted from the fu…

    Promoter

    Available since

    July 24, 2013

    Availability

    Academic Institutions and Nonprofits only

13. mClY-N1

    Plasmid

    #90457

    Purpose

    A YFP-based chloride sensor with enhanced chloride sensitivity, photostability and reduced pH interference. The sequence of mClY is EYFP-F46L/Q69K/H148Q/I152L/V163S/S175G/S205V/A206K.

    Depositor

    Joseph Santos-Sacchi

    Article

    Zhong et al PLoS One. 2014 Jun 5;9(6):e99095. doi: 1

    Insert

    mClY-N1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    EYFP-F46L/Q69K/H148Q/I152L/V163S/S175G/S205V/A206…

    Promoter

    Available since

    May 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGL4.0-TERT WT

    Plasmid

    #84924

    Purpose

    luciferase reporter for TERT promoter

    Depositor

    Joseph Costello

    Article

    Bell et al Science. 2015 May 29;348(6238):1036-9. d

    Insert

    TERT promoter (TERT Human)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    TERT

    Available since

    March 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGL4.0-TERT G228A

    Plasmid

    #84926

    Purpose

    luciferase reporter for mutant TERT promoter

    Depositor

    Joseph Costello

    Article

    Bell et al Science. 2015 May 29;348(6238):1036-9. d

    Insert

    TERT promoter (TERT Human)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    G228A

    Promoter

    TERT

    Available since

    March 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGL4.0-TERT G250A

    Plasmid

    #84925

    Purpose

    luciferase reporter for mutant TERT promoter

    Depositor

    Joseph Costello

    Article

    Bell et al Science. 2015 May 29;348(6238):1036-9. d

    Insert

    TERT promoter (TERT Human)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    G250A

    Promoter

    TERT

    Available since

    March 8, 2017

    Availability

    Academic Institutions and Nonprofits only

17. pMMB67EH-dddAI

    Plasmid

    #186559

    Purpose

    Broad host range regulated expression of the DddA immunity determinant (DddAI)

    Depositor

    Joseph Mougous

    Article

    Gallagher et al Nat Microbiol. 2022 Jun;7(6):844-855. do

    Insert

    dddAI
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-H2B-tdTomato-SRT

    Plasmid

    #203393

    Purpose

    AAV transfer plasmid containing H2B fused piggyBac self-reporting transposon with tdTomato marker for mammalian calling cards

    Depositor

    Joseph Dougherty, Rob Mitra

    Article

    Yen et al Curr Protoc. 2023 Sep;3(9):e883. doi: 10

    Insert

    H2B fused piggyBac tdTomato self-reporting transposon
    

    Use

    AAV

    Tags

    3X SV40 and H2B

    Expression

    Mammalian

    Mutation

    Promoter

    Ef1a

    Available since

    July 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pUC19-U6-BsmBI_cassette-Nme/Nme2Cas9-sgRNA (KAC32)

    Plasmid

    #133794

    Purpose

    U6 promoter sgRNA entry vector used for all NmeCas9 or Nme2Cas9 sgRNAs (clone spacer oligos into BsmBI cassette) - similar to sgRNA architecture from Amrani et al. Genome Biology 2018

    Depositor

    Joseph Bondy-Denomy, Benjamin Kleinstiver

    Article

    Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

    Insert

    NmeCas9 and Nme2Cas9 sgRNA entry vector
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    sgRNA architecture from Amrani et al. Genome Biol…

    Promoter

    U6

    Available since

    March 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pmCherry-Cep290

    Plasmid

    #27380

    Depositor

    Joseph Gleeson

    Article

    Valente et al Nat Genet. 2006 Jun . 38(6):623-5.

    Insert

    Cep290 (Cep290 Mouse)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    please see depositor comments below

    Promoter

    Available since

    June 1, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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