We narrowed to 2,493 results for: ERK

1. pGAL4-DBD-OLIG2-Adel-IDR

   Plasmid

   #145249

   Purpose

   Expresses fusion of GAL4 DNA-binding domain and OLIG2-Adel IDR

   Depositor

   Denes Hnisz

   Article

   Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

   Insert

   OLIG2-Adel-IDR (OLIG2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   A-repeats deleted

   Promoter

   Available since

   Nov. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pGAL4-DBD-HOXD11wt-IDR

   Plasmid

   #145242

   Purpose

   Expresses fusion of GAL4 DNA-binding domain and HOXD11wt-IDR

   Depositor

   Denes Hnisz

   Article

   Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

   Insert

   HOXD11wt-IDR (HOXD11 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pGAL4-DBD-MNX1-Adel-IDR

   Plasmid

   #145247

   Purpose

   Expresses fusion of GAL4 DNA-binding domain and MNX1-Adel IDR

   Depositor

   Denes Hnisz

   Article

   Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

   Insert

   MNX1-Adel-IDR (MNX1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   A-repeats deleted

   Promoter

   Available since

   Sept. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pGAL4-DBD-TBX1-Adel-IDR

   Plasmid

   #145253

   Purpose

   Expresses fusion of GAL4 DNA-binding domain and TBX1-Adel IDR

   Depositor

   Denes Hnisz

   Article

   Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

   Insert

   TBX1-Adel-IDR (TBX1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   A-repeats deleted

   Promoter

   Available since

   Sept. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. 24xMoonTag-kif18b-24xPP7 (MoonTag translation reporter)

   Plasmid

   #128604

   Purpose

   Expresses 24xgp41 peptide array (MoonTag peptide array), followed by kif18b gene and 24 repeats of PP7 hairpins in 3’ UTR.

   Depositor

   Marvin Tanenbaum

   Article

   Boersma et al Cell. 2019 Jun 5. pii: S0092-8674(19)304

   Inserts

   24xMoonTag
   kinesin family member 18b (KIF18B Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Pcmv

   Available since

   Aug. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. H3K9me3 biosensor

   Plasmid

   #120802

   Purpose

   FRET biosensor. To monitor histone H3 lysine 9 tri-methylation in mammalian cells by FRET

   Depositor

   Yingxiao Wang

   Article

   Peng et al Proc Natl Acad Sci U S A. 2018 Nov 26. p

   Insert

   Truncated YPet-HP1-EV linker-ECFP-mouse histone H3
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. HS-GFP-PACT

   Plasmid

   #105954

   Purpose

   heat shock inducible transgenesis, PACT is a centriol marker

   Depositor

   Caren Norden

   Article

   Strzyz et al Dev Cell. 2015 Jan 26;32(2):203-19. doi:

   Insert

   PACT (AKAP9 Human)
   

   Use

   Tags

   GFP

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. p2K7-bsd-UBI-tagRFP-KDEL

   Plasmid

   #114179

   Purpose

   Lentiviral vector for expression of tagRFP with a signal peptide and KDEL ER retention signal for expression of tagRFP in the ER

   Depositor

   Nicolas Demaurex

   Article

   Nunes-Hasler et al Nat Commun. 2017 Nov 24;8(1):1852. doi:

   Insert

   tagRFP-KDEL
   

   Use

   Lentiviral

   Tags

   KDEL retention signal and signal peptide

   Expression

   Mammalian

   Mutation

   Promoter

   Ubiquitin

   Available since

   Sept. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pLHCX-XBP1 mNeonGreen NLS

   Plasmid

   #115971

   Purpose

   ER-stress sensor - XBP1 splicing fluorescent reporter with mNeonGreen (retroviral vector backbone)

   Depositor

   David Andrews

   Article

   Nougarede et al Assay Drug Dev Technol. 2018 Aug 8. doi:

   Insert

   X-box binding protein 1 (XBP1 Synthetic, Human)
   

   Use

   Retroviral

   Tags

   HA tag, c-myc NLS, and mNeonGreen

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pHR-RUNX2(+10A)-IDR-mCherry-CRY2-NLS

    Plasmid

    #145268

    Purpose

    Mammalian Expression of Nucelar Opto RUNX2(+10A) IDR with SV40 NLS

    Depositor

    Denes Hnisz

    Article

    Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

    Insert

    RUNX2(+10A)-IDR (RUNX2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    A-repeat expanded

    Promoter

    Available since

    Sept. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. TVBB C-term-mScarlet

    Plasmid

    #169219

    Purpose

    Targeting vector backbone to support a knock-in of Linker-mScarlet at the C-terminus of a target locus

    Depositor

    Hugo Snippert

    Article

    Bollen et al PLoS Biol. 2022 Jan 28;20(1):e3001527. d

    Insert

    Double SapI flanked mScarlet
    

    Use

    Targeting vector backbone

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. WT Smarca4-sfGFP

    Plasmid

    #107056

    Purpose

    Expression of wild-type Smarca4 (Brg1) with C-terminal super-folder GFP fusion.

    Depositor

    Courtney Hodges, Jerry Crabtree

    Article

    Hodges et al Nat Struct Mol Biol. 2018 Jan;25(1):61-7

    Insert

    SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 (Smarca4 Mouse)
    

    Use

    Lentiviral

    Tags

    Super-folder GFP (sfGFP)

    Expression

    Mammalian

    Mutation

    wild-type

    Promoter

    CAG

    Available since

    April 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA-hDDB2-WT-V5

    Plasmid

    #167468

    Purpose

    ectopic expression of hDDB2 in mammalian cells

    Depositor

    Steven Artandi

    Article

    Chen et al DNA Repair (Amst). 2021 Jan;97:103022. d

    Insert

    DDB2 (DDB2 Human)
    

    Use

    Tags

    6xHis and V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pEv-Fat4-citrine

    Plasmid

    #112854

    Purpose

    Constitutive expression of human fat4 protein fused to citrine fluorescent protein

    Depositor

    David Sprinzak

    Article

    Loza et al Elife. 2017 Aug 18;6. pii: 24820. doi: 1

    Insert

    FAT4 (FAT4 Human)
    

    Use

    Tags

    Citrine

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAAV CD68-hM4D(Gi)-mCherry

    Plasmid

    #75033

    Purpose

    Expression of hM4D(Gi) using the CD68 promoter.

    Depositor

    Bryan Roth

    Article

    Grace et al Proc Natl Acad Sci U S A. 2016 Jun 14;11

    Has Service

    AAV1 and AAV9

    Insert

    hM4D(Gi)-mCherry
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CD68

    Available since

    Oct. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pTreTight-Htt94Q-CFP

    Plasmid

    #23966

    Depositor

    Nico Dantuma

    Article

    Maynard et al Proc Natl Acad Sci U S A. 2009 Aug 18. 1

    Insert

    N-terminal fragment Huntingtin Q94 (HTT Human)
    

    Use

    Tags

    CFP

    Expression

    Mammalian

    Mutation

    94 polyQ repeat

    Promoter

    Available since

    April 15, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. WT Smarca4-V5 IRES-puro

    Plasmid

    #107058

    Purpose

    Expression of wild-type Smarca4 (Brg1)-V5 fusion with IRES puromycin resistance.

    Depositor

    Courtney Hodges, Jerry Crabtree

    Article

    Hodges et al Nat Struct Mol Biol. 2018 Jan;25(1):61-7

    Insert

    SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 (Smarca4 Mouse)
    

    Use

    Lentiviral

    Tags

    IRES-puroR and V5

    Expression

    Mammalian

    Mutation

    wild-type

    Promoter

    CAG

    Available since

    April 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLS13-IL-10

    Plasmid

    #209128

    Purpose

    Transient mammalian expression of IL-10

    Depositor

    Tom de Greef

    Article

    Makri Pistikou et al Nat Commun. 2023 Nov 2;14(1):7001. doi:

    Insert

    IL-10 (IL10 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. H3S10ph biosensor

    Plasmid

    #120809

    Purpose

    FRET biosensor. To monitor histone H3 Serine 10 phosphorylation in mammalian cells by FRET

    Depositor

    Yingxiao Wang

    Article

    Peng et al Proc Natl Acad Sci U S A. 2018 Nov 26. p

    Insert

    Mouse histone H3-CFP-FHA2-histone H3 peptide (1-14aa)-YFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Threonine 3, Threonine 6, and Threonine 11 are mu…

    Promoter

    CMV

    Available since

    Feb. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCMV-DeAct-SpvB

    Plasmid

    #89446

    Purpose

    Expresses EGFP-SpvB (Salmonella SpvB 375-591) in mammalian cells

    Depositor

    Brad Zuchero

    Article

    Harterink et al Nat Methods. 2017 Apr 10. doi: 10.1038/n

    Insert

    DeAct-SpvB (spvB Salmonella enterica)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Truncation spanning SpvB amino acids 375-591 (mon…

    Promoter

    CMV

    Available since

    April 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. K785R Smarca4-sfGFP

    Plasmid

    #107057

    Purpose

    Expression of dominant-negative K785R Smarca4 (Brg1) with C-terminal super-folder GFP fusion.

    Depositor

    Courtney Hodges, Jerry Crabtree

    Article

    Hodges et al Nat Struct Mol Biol. 2018 Jan;25(1):61-7

    Insert

    SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 (Smarca4 Mouse)
    

    Use

    Lentiviral

    Tags

    Super-folder GFP (sfGFP)

    Expression

    Mammalian

    Mutation

    K785R

    Promoter

    CAG

    Available since

    April 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. TVBB C-term-mNeongreen

    Plasmid

    #169227

    Purpose

    Targeting vector backbone to support a knock-in of Linker-mNeongreen at the C-terminus of a target locus

    Depositor

    Hugo Snippert

    Article

    Bollen et al PLoS Biol. 2022 Jan 28;20(1):e3001527. d

    Insert

    Double SapI flanked mNeongreen
    

    Use

    Targeting vector backbone

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. K785R Smarca4-V5 IRES-puro

    Plasmid

    #107059

    Purpose

    Expression of dominant-negative K785R Smarca4 (Brg1)-V5 fusion with IRES puromycin resistance.

    Depositor

    Courtney Hodges, Jerry Crabtree

    Article

    Hodges et al Nat Struct Mol Biol. 2018 Jan;25(1):61-7

    Insert

    SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 (Smarca4 Mouse)
    

    Use

    Lentiviral

    Tags

    IRES-puroR and V5

    Expression

    Mammalian

    Mutation

    K785R

    Promoter

    CAG

    Available since

    April 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. TVBB N-term-mScarlet

    Plasmid

    #169218

    Purpose

    Targeting vector backbone to support a knock-in of mScarlet-Linker at the N-terminus of a target locus

    Depositor

    Hugo Snippert

    Article

    Bollen et al PLoS Biol. 2022 Jan 28;20(1):e3001527. d

    Insert

    Double SapI flanked mScarlet
    

    Use

    Targeting vector backbone

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pET-45b-HOXD13(+7A)-IDR-mCherry

    Plasmid

    #145281

    Purpose

    Bacterial Expression of 6xHis HOXD13 (+7A) IDR mCherry Fusion Protein

    Depositor

    Denes Hnisz

    Article

    Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

    Insert

    HOXD13(+7A)-IDR (HOXD13 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    A-repeat expanded

    Promoter

    Available since

    Sept. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pYX233-DHFR

    Plasmid

    #163761

    Purpose

    Gal-inducible yeast expression of cytosolic DHFR

    Depositor

    Johannes Herrmann

    Article

    Boos et al Nat Cell Biol. 2019 Apr;21(4):442-451. d

    Insert

    DHFR (Dhfr Mouse)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available since

    Jan. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pTetON-DeAct-SpvB

    Plasmid

    #89455

    Purpose

    Tet/Dox-inducible expression of EGFP-SpvB (Salmonella SpvB 375-591) in mammalian cells

    Depositor

    Brad Zuchero

    Article

    Harterink et al Nat Methods. 2017 Apr 10. doi: 10.1038/n

    Insert

    DeAct-SpvB (spvB Salmonella enterica)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Truncation spanning SpvB amino acids 375-591 (mon…

    Promoter

    TRE3G

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCIG-LRRC37Bchimpanzee-HA

    Plasmid

    #220794

    Purpose

    To express the chimpanzee receptor LRRC37B in mammalian cells together with EGFP

    Depositor

    Pierre Vanderhaeghen

    Article

    Libe-Philippot et al Cell. 2023 Dec 21;186(26):5766-5783.e25.

    Insert

    LRRC37B
    

    Use

    Tags

    HA

    Expression

    Mutation

    Promoter

    Available since

    Aug. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. TVBB N-term-mTurquoise2

    Plasmid

    #169222

    Purpose

    Targeting vector backbone to support a knock-in of mTurquoise2-Linker at the N-terminus of a target locus

    Depositor

    Hugo Snippert

    Article

    Bollen et al PLoS Biol. 2022 Jan 28;20(1):e3001527. d

    Insert

    Double SapI flanked mTurquoise2
    

    Use

    Targeting vector backbone

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. TVBB C-term-mTurquoise2

    Plasmid

    #169223

    Purpose

    Targeting vector backbone to support a knock-in of Linker-mTurquoise2 at the C-terminus of a target locus

    Depositor

    Hugo Snippert

    Article

    Bollen et al PLoS Biol. 2022 Jan 28;20(1):e3001527. d

    Insert

    Double SapI flanked mTurquoise2
    

    Use

    Targeting vector backbone

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. tdKatushka2-Fibrillarin-7

    Plasmid

    #56041

    Purpose

    Localization: Nucleoli, Excitation: 586, Emission: 632

    Depositor

    Michael Davidson

    Article

    Shcherbo et al Biochem J. 2009 Mar 15;418(3):567-74. do

    Insert

    Fibrillarin (FBL Human)
    

    Use

    Tags

    tdKatushka2

    Expression

    Mammalian

    Mutation

    R36Q, S66F

    Promoter

    CMV

    Available since

    April 1, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

32. TVBB N-term-mNeongreen

    Plasmid

    #169226

    Purpose

    Targeting vector backbone to support a knock-in of mNeongreen-Linker at the N-terminus of a target locus

    Depositor

    Hugo Snippert

    Article

    Bollen et al PLoS Biol. 2022 Jan 28;20(1):e3001527. d

    Insert

    Double SapI flanked mNeongreen
    

    Use

    Targeting vector backbone

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pET-45b-HOXD13-IDR-mCherry

    Plasmid

    #145280

    Purpose

    Bacterial Expression of 6xHis HOXD13 IDR mCherry Fusion Protein

    Depositor

    Denes Hnisz

    Article

    Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

    Insert

    HOXD13-IDR (HOXD13 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pDEST-mCherry-LacR-BRCA1 1366-1437

    Plasmid

    #71118

    Purpose

    mammalian expession of mCherry-LacR-BRCA1 1366-1437

    Depositor

    Daniel Durocher

    Article

    Orthwein et al Nature. 2015 Dec 9. doi: 10.1038/nature1

    Insert

    BRCA1 (BRCA1 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    contains amino acids 1366-1437 of BRCA1

    Promoter

    CMV/TO

    Available since

    April 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pHR-HOXD13-IDR-mCherry-CRY2

    Plasmid

    #145258

    Purpose

    Mammalian Expression of Opto HOXD13 IDR

    Depositor

    Denes Hnisz

    Article

    Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

    Insert

    HOXD13wt-IDR (HOXD13 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pHR-RUNX2-IDR-mCherry-CRY2-NLS

    Plasmid

    #145267

    Purpose

    Mammalian Expression of Nuclear Opto RUNX2 IDR with SV40 NLS

    Depositor

    Denes Hnisz

    Article

    Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

    Insert

    RUNX2wt-IDR (RUNX2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pCFP-LacI-HOXD13wt-IDR

    Plasmid

    #145255

    Purpose

    Expresses fusion of ECFP-LacI and HOXD13wt IDR

    Depositor

    Denes Hnisz

    Article

    Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

    Insert

    HOXD13wt-IDR (HOXD13 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. MKV937/nlp-1p::FlincG3

    Plasmid

    #140508

    Purpose

    This construct contains cGMP biosensor driven by nlp-1 promoter for expression in PHB sensory neuron in pSMdelta backbone.

    Depositor

    Miri VanHoven

    Article

    Woldemariam et al Genetics. 2019 Jul 22. pii: genetics.119

    Insert

    FlincG3
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    nlp-1

    Available since

    May 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pCMV NucleoBAS N354K-I372V

    Plasmid

    #63574

    Purpose

    Fluorescent bile acid sensor (BAS) targeted to nucleus that allows spatiotemporal monitoring of bile acid transport in single living cells using FRET

    Depositor

    Stan van de Graaf

    Article

    van der Velden et al Hepatology. 2013 Feb;57(2):740-52. doi:

    Insert

    NucleoBAS N354K-I372V
    

    Use

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    N345K and I372V

    Promoter

    CMV

    Available since

    May 11, 2015

    Availability

    Academic Institutions and Nonprofits only

40. mKate1.3-Fibrillarin-7

    Plasmid

    #56084

    Purpose

    Localization: Nucleoli, Excitation: 588, Emission: 635

    Depositor

    Michael Davidson

    Article

    Shcherbo et al Biochem J. 2009 Mar 15;418(3):567-74. do

    Insert

    Fibrillarin (FBL Human)
    

    Use

    Tags

    mKate1.3

    Expression

    Mammalian

    Mutation

    R36Q, S66F

    Promoter

    CMV

    Available since

    April 1, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits