We narrowed to 12,153 results for: 112

1. pNR1D2-donor

   Plasmid

   #112314

   Purpose

   CRISPR donor plasmid to tag human transcription factor NR1D2 with GFP

   Depositor

   Kevin White

   Insert

   NR1D2 homology arms flanking EGFP-IRES-Neo cassette (NR1D2 Human)
   

   Use

   CRISPR

   Mutation

   hg38:3:23977483:A>T, hg38:3:23977515:A>G, h…

   Available Since

   Nov. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pSMAD3-donor

   Plasmid

   #112311

   Purpose

   CRISPR donor plasmid to tag human transcription factor SMAD3 with GFP

   Depositor

   Kevin White

   Insert

   SMAD3 homology arms flanking EGFP-IRES-Neo cassette (SMAD3 Human)
   

   Use

   CRISPR

   Mutation

   hg38:15:67189762:C>T, rs11629568, rs8025774

   Available Since

   Nov. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pZNF354B-donor

   Plasmid

   #112309

   Purpose

   CRISPR donor plasmid to tag human transcription factor ZNF354B with GFP

   Depositor

   Kevin White

   Insert

   ZNF354B homology arms flanking EGFP-IRES-Neo cassette (ZNF354B Human)
   

   Use

   CRISPR

   Mutation

   hg38:5:178884427:G>A, hg38:5:178883445:C>T,…

   Available Since

   Nov. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pZNF75A-donor

   Plasmid

   #112307

   Purpose

   CRISPR donor plasmid to tag human transcription factor ZNF75A with GFP

   Depositor

   Kevin White

   Insert

   ZNF75A homology arms flanking EGFP-IRES-Neo cassette (ZNF75A Human)
   

   Use

   CRISPR

   Mutation

   hg38:16:3317462:A>T, hg38:16:3317715:C>T, h…

   Available Since

   Nov. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pZNF266-donor

   Plasmid

   #112306

   Purpose

   CRISPR donor plasmid to tag human transcription factor ZNF266 with GFP

   Depositor

   Kevin White

   Insert

   ZNF266 homology arms flanking EGFP-IRES-Neo cassette (ZNF266 Human)
   

   Use

   CRISPR

   Mutation

   Unknown

   Available Since

   Nov. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pZNF507-donor

   Plasmid

   #112305

   Purpose

   CRISPR donor plasmid to tag human transcription factor ZNF507 with GFP

   Depositor

   Kevin White

   Insert

   ZNF507 homology arms flanking EGFP-IRES-Neo cassette (ZNF507 Human)
   

   Use

   CRISPR

   Mutation

   rs2270399, hg38:19:32382978:A>T, rs2111503

   Available Since

   Nov. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pZSCAN32-donor

   Plasmid

   #112304

   Purpose

   CRISPR donor plasmid to tag human transcription factor ZNF434 with GFP

   Depositor

   Kevin White

   Insert

   ZNF434 homology arms flanking EGFP-IRES-Neo cassette (ZSCAN32 Human)
   

   Use

   CRISPR

   Available Since

   Nov. 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pZNF165-donor

   Plasmid

   #112302

   Purpose

   CRISPR donor plasmid to tag human transcription factor ZNF165 with GFP

   Depositor

   Kevin White

   Insert

   ZNF165 homology arms flanking EGFP-IRES-Neo cassette (ZNF165 Human)
   

   Use

   CRISPR

   Mutation

   Unknown

   Available Since

   Nov. 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pZNF764-donor

   Plasmid

   #112298

   Purpose

   CRISPR donor plasmid to tag human transcription factor ZNF764 with GFP

   Depositor

   Kevin White

   Insert

   ZNF764 homology arms flanking EGFP-IRES-Neo cassette (ZNF764 Human)
   

   Use

   CRISPR

   Mutation

   hg38:16:30555233:G>A, hg38:16:30555234:C>G,…

   Available Since

   Nov. 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCR-Blunt_mWasabi

    Plasmid

    #112640

    Purpose

    Plasmid for PCR amplification of mWasabi DNA template for in vitro transcription

    Depositor

    Ganna Bilousova

    Article

    McGrath et al J Vis Exp. 2018 Nov 26;(141). doi: 10.37

    Insert

    mWasabi
    

    Use

    Pcr template

    Promoter

    T7

    Available Since

    Nov. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. FFTTH-Amotl2

    Plasmid

    #112832

    Purpose

    transient/stable overexpression

    Depositor

    Tomasz Prószyński

    Insert

    Amotl2 (Amotl2 Mouse)
    

    Expression

    Mammalian

    Available Since

    Nov. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pRK5 Flag RagC (D181N)

    Plasmid

    #112752

    Purpose

    express Flag-RagC (D181N mutant)

    Depositor

    Roberto Zoncu

    Article

    Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

    Insert

    Flag-RagC (D181N) (RRAGC Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    181N

    Available Since

    Oct. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLL5 sgRNA(MS2)-MS2-hA3a D131A

    Plasmid

    #112131

    Purpose

    empty sgRNA cloning vector with MS2-humanA3a D131A

    Depositor

    Fei-Long Meng

    Article

    Liu et al Cell Rep. 2018 Oct 23;25(4):884-892.e3.

    Insert

    human Apobec3a D131A mutant (APOBEC3A Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    D131A mutant in hA3a

    Promoter

    hU6,CBh

    Available Since

    Sept. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pmTHC_PGKneoLox2DTA.2

    Plasmid

    #112624

    Purpose

    H2BmTeal_p2A_HygroR_p2A_CreERt2 targeting plasmid with cloning sites ready for homology arms

    Depositor

    Stefan Heller

    Article

    Hartman et al Dev Biol. 2018 Sep 1. pii: S0012-1606(18

    Inserts

    H2B-mTFP1 (H2BC3 Human, Anthozoa)
    Hygrophosphotransferase
    Cre-ERT2 fusion protein
    

    Use

    Cre/Lox and Mouse Targeting

    Tags

    mTeal (mTFP1)

    Promoter

    No Promoter

    Available Since

    Sept. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pEHC_PGKneoLox2DTA.2

    Plasmid

    #112623

    Purpose

    H2BEmerald_p2A_HygroR_p2A_CreERt2 targeting plasmid with cloning sites ready for homology arms

    Depositor

    Stefan Heller

    Article

    Hartman et al Dev Biol. 2018 Sep 1. pii: S0012-1606(18

    Inserts

    H2B-Emerald (H2BC3 Human, Aequorea victoria)
    Hygrophosphotransferase
    Cre-ERT2 fusion protein
    

    Use

    Cre/Lox and Mouse Targeting

    Tags

    Emerald

    Promoter

    No Promoter

    Available Since

    Sept. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pQE80L TriCatcher-MMP

    Plasmid

    #112632

    Purpose

    Two SpyCatchers linked by an elastin-like polypeptide with an MMP-cleavable sequence and a C-terminal SnoopCatcher

    Depositor

    Mark Howarth

    Article

    Wieduwild et al Biomaterials. 2018 Oct;180:253-264. doi:

    Insert

    TriCatcher-MMP
    

    Tags

    His6 and TEV cleavage site

    Expression

    Bacterial

    Mutation

    central matrix metalloproteinase-cleavable sequen…

    Promoter

    T5

    Available Since

    Sept. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pGAT

    Plasmid

    #112587

    Purpose

    Used for expression of protein in E. coli as Thrombin cleavable 6His-tag-GST fusions with a blunt end restriction site immediately after thrombin recognition sequence

    Depositor

    Johan Peränen, Marko Hyvönen

    Article

    Peranen et al Anal Biochem. 1996 May 1;236(2):371-3.

    Type

    Empty backbone

    Tags

    6His-GST

    Expression

    Bacterial

    Available Since

    Sept. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. GST-GNU-N

    Plasmid

    #112972

    Purpose

    Expresses fusion of GST and N terminal amino acids of GNU

    Depositor

    Terry Orr-Weaver

    Article

    Lee et al Genes Dev. 2003 Dec 1;17(23):2979-91. do

    Insert

    gnu (gnu Fly)
    

    Expression

    Bacterial

    Available Since

    Sept. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. mei-S332 5.6Kb genomic DNA in Casper4

    Plasmid

    #112984

    Purpose

    P element vector for transposon insertion of mei-S332 5.6Kb genomic DNA into Drosophila genome

    Depositor

    Terry Orr-Weaver

    Article

    Kerrebrock et al Cell. 1995 Oct 20;83(2):247-56.

    Insert

    mei-S332 (Sgo) (mei-S332 Fly)
    

    Use

    P insertion vector

    Tags

    GFP

    Available Since

    Sept. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. LSS-SGFP2-aTub

    Plasmid

    #112936

    Purpose

    Visualization of microtubules

    Depositor

    Dorus Gadella

    Insert

    alpha tubulin
    

    Tags

    LSS-SGFP2

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge