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Plasmid#112306PurposeCRISPR donor plasmid to tag human transcription factor ZNF266 with GFPDepositorInsertZNF266 homology arms flanking EGFP-IRES-Neo cassette (ZNF266 Human)
UseCRISPRTagsExpressionMutationUnknownPromoterAvailable sinceNov. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pZNF507-donor
Plasmid#112305PurposeCRISPR donor plasmid to tag human transcription factor ZNF507 with GFPDepositorInsertZNF507 homology arms flanking EGFP-IRES-Neo cassette (ZNF507 Human)
UseCRISPRTagsExpressionMutationrs2270399, hg38:19:32382978:A>T, rs2111503PromoterAvailable sinceNov. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pZSCAN32-donor
Plasmid#112304PurposeCRISPR donor plasmid to tag human transcription factor ZNF434 with GFPDepositorInsertZNF434 homology arms flanking EGFP-IRES-Neo cassette (ZSCAN32 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceNov. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pZNF165-donor
Plasmid#112302PurposeCRISPR donor plasmid to tag human transcription factor ZNF165 with GFPDepositorInsertZNF165 homology arms flanking EGFP-IRES-Neo cassette (ZNF165 Human)
UseCRISPRTagsExpressionMutationUnknownPromoterAvailable sinceNov. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pZNF764-donor
Plasmid#112298PurposeCRISPR donor plasmid to tag human transcription factor ZNF764 with GFPDepositorInsertZNF764 homology arms flanking EGFP-IRES-Neo cassette (ZNF764 Human)
UseCRISPRTagsExpressionMutationhg38:16:30555233:G>A, hg38:16:30555234:C>G,…PromoterAvailable sinceNov. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCR-Blunt_mWasabi
Plasmid#112640PurposePlasmid for PCR amplification of mWasabi DNA template for in vitro transcriptionDepositorInsertmWasabi
UsePcr templateTagsExpressionMutationPromoterT7Available sinceNov. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
FFTTH-Amotl2
Plasmid#112832Purposetransient/stable overexpressionDepositorInsertAmotl2 (Amotl2 Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRK5 Flag RagC (D181N)
Plasmid#112752Purposeexpress Flag-RagC (D181N mutant)DepositorInsertFlag-RagC (D181N) (RRAGC Human)
UseTagsFlagExpressionMammalianMutation181NPromoterAvailable sinceOct. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLL5 sgRNA(MS2)-MS2-hA3a D131A
Plasmid#112131Purposeempty sgRNA cloning vector with MS2-humanA3a D131ADepositorInserthuman Apobec3a D131A mutant (APOBEC3A Human)
UseCRISPRTagsExpressionMammalianMutationD131A mutant in hA3aPromoterhU6,CBhAvailable sinceSept. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pmTHC_PGKneoLox2DTA.2
Plasmid#112624PurposeH2BmTeal_p2A_HygroR_p2A_CreERt2 targeting plasmid with cloning sites ready for homology armsDepositorInsertsUseCre/Lox and Mouse TargetingTagsmTeal (mTFP1)ExpressionMutationPromoterNo PromoterAvailable sinceSept. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEHC_PGKneoLox2DTA.2
Plasmid#112623PurposeH2BEmerald_p2A_HygroR_p2A_CreERt2 targeting plasmid with cloning sites ready for homology armsDepositorInsertsUseCre/Lox and Mouse TargetingTagsEmeraldExpressionMutationPromoterNo PromoterAvailable sinceSept. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pQE80L TriCatcher-MMP
Plasmid#112632PurposeTwo SpyCatchers linked by an elastin-like polypeptide with an MMP-cleavable sequence and a C-terminal SnoopCatcherDepositorInsertTriCatcher-MMP
UseTagsHis6 and TEV cleavage siteExpressionBacterialMutationcentral matrix metalloproteinase-cleavable sequen…PromoterT5Available sinceSept. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGAT
Plasmid#112587PurposeUsed for expression of protein in E. coli as Thrombin cleavable 6His-tag-GST fusions with a blunt end restriction site immediately after thrombin recognition sequenceDepositorTypeEmpty backboneUseTags6His-GSTExpressionBacterialMutationPromoterAvailable sinceSept. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
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mei-S332 5.6Kb genomic DNA in Casper4
Plasmid#112984PurposeP element vector for transposon insertion of mei-S332 5.6Kb genomic DNA into Drosophila genomeDepositorInsertmei-S332 (Sgo) (mei-S332 Fly)
UseP insertion vectorTagsGFPExpressionMutationPromoterAvailable sinceSept. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSS-SGFP2-aTub
Plasmid#112936PurposeVisualization of microtubulesDepositorInsertalpha tubulin
UseTagsLSS-SGFP2ExpressionMammalianMutationPromoterCMVAvailable sinceAug. 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pNAS1b split mCherry NEDD4 WW2 neg ctrl
Plasmid#112030PurposeMode #2 phosphosite negative control (split mCherry, using NEDD4 WW2 binding domain paired with negative control peptide)DepositorInsertNEDD4 WW2 domain fused to C-terminal split mCherry, neg ctrl interacting peptide fused to N-terminal split mCherry
UseTagsSplit mCherryExpressionBacterialMutationPromoterAvailable sinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pOP5TT
Plasmid#112613PurposeUsed for expression of protein in E. coli as TEV cleavable N-terminual 8His and thioredoxin tag fusions and with C-terminal Avi-tagDepositorTypeEmpty backboneUseTags8His-thioredoxin and AviExpressionBacterialMutationPromoterAvailable sinceAug. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pOP5BP
Plasmid#112608PurposeUsed for expression of protein in E. coli as PreScission cleavable N-terminual 8His and GB1 tag fusions and with C-terminal Avi-tagDepositorTypeEmpty backboneUseTags8His-GB1 and AviExpressionBacterialMutationPromoterAvailable sinceAug. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pOP3GP
Plasmid#112606PurposeUsed for expression of protein in E. coli as PreScission cleavable N-terminal Avi-His8-tag and GST fusionsDepositorTypeEmpty backboneUseTagsAvi - 8His-GSTExpressionBacterialMutationPromoterAvailable sinceAug. 27, 2018AvailabilityAcademic Institutions and Nonprofits only