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pBI-CMV1-mir451~144-CMV2-mir1

Plasmid

#164666

Purpose

Express miR-451~144 and miR-1-1 in mammalian cells

Depositor

David Bartel

Article

Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

Inserts

miR-451~144 (MIR451A Human)
miR-1-1 (MIR1-1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 29, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-hPGK-mCherry-SynaptoZip

Plasmid

#177317

Purpose

Lentiviral expression of the synaptic activity-marker SynaptoZip fused to mCherry driven by hPGK promoter

Depositor

Antonio Malgaroli

Article

Lamanna et al Sci Rep. 2022 Jun 30;12(1):11055. doi: 1

Insert

mCherry-SynaptoZip (Vamp2 Rat)

Use

Lentiviral

Tags

Myc and mCherry

Expression

Mammalian

Mutation

Promoter

Available since

June 24, 2022

Availability

Academic Institutions and Nonprofits only

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pKS18

Plasmid

#24629

Depositor

Jay Keasling

Article

Steen et al Nature. 2010 Jan 28;463(7280):559-62.

Insert

mFar1 (Far1 Mouse)

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 11, 2010

Availability

Academic Institutions and Nonprofits only

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pCDNA4/TO-GFP-SPOP (WT)

Plasmid

#197008

Purpose

Mammalian inducible expression vector for GFP-tagged SPOP

Depositor

Tanja Mittag

Article

Sabri et al J Biol Chem. 2023 Dec;299(12):105427. do

Insert

SPOP (Spop Mouse)

Use

Tags

GFP

Expression

Mammalian

Mutation

-

Promoter

Available since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

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SF-U7snRNA-CypA-E4

Plasmid

#190695

Purpose

Control vector expressing a U7-based antisense oligonucleotide targeting CypA exon 4

Depositor

Pim Pijnappel

Article

van der Wal et al Mol Ther Nucleic Acids. 2017 Jun 16;7:90

Insert

U7 snRNA antisense CypA Exon 4

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U7 promoter

Available since

April 11, 2023

Availability

Academic Institutions and Nonprofits only

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pHAGE2-TetOminiCMV-Oct4

Plasmid

#136611

Purpose

Dox-inducible lentiviral vector expressing mouse Oct4

Depositor

Hans Schöler

Article

Velychko et al Cell Stem Cell. 2019 Oct 30. pii: S1934-

Insert

Oct4 (Pou5f1 Mouse)

Use

Lentiviral

Tags

Expression

Bacterial

Mutation

Promoter

tetO-miniCMV (dox-inducible)

Available since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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pDECKO_TFRC_C (with mCherry)

Plasmid

#72627

Purpose

Expresses two gRNAs targeting the TFRC promoter

Depositor

Rory Johnson, Roderic Guigo

Article

Aparicio-Prat et al BMC Genomics. 2015 Oct 23;16(1):846. doi

Insert

gRNAs toward TFRC

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6 (gRNA1) and H1 (gRNA2)

Available since

June 14, 2016

Availability

Academic Institutions and Nonprofits only

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MigR1 Fli-1 GFP

Plasmid

#24668

Depositor

Yaacov Ben-David

Article

Cui et al Blood. 2007 Oct 1. 110(7):2631-40.

Insert

Fli-1 (Fli1 Mouse)

Use

Retroviral

Tags

GFP and IRES

Expression

Mutation

Promoter

Available since

May 6, 2010

Availability

Academic Institutions and Nonprofits only
UKNP.1.12.1

Plasmid

#97384

Purpose

Expression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)

Depositor

Jonathan Ball

Article

Urbanowicz et al J Virol. 2015 Dec 23;90(7):3288-301. doi

Insert

HCV E1E2

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pHisAlix deltaPRD

Plasmid

#42577

Depositor

James Hurley

Article

Carlson et al Proc Natl Acad Sci U S A. 2012 Oct 16;10

Insert

delta-PRD ALIX (residues 1-702) (PDCD6IP Human)

Use

Tags

HIS-TEV

Expression

Bacterial

Mutation

silent mutation A1617G (numbering in ALIX gene)

Promoter

Available since

Dec. 19, 2013

Availability

Academic Institutions and Nonprofits only
Actin.CIB.GFP

Plasmid

#133618

Purpose

Expresses beta actin - CIBN - GFP protein fusion

Depositor

Robert Hughes

Article

Salem et al J Biol Chem. 2020 Aug 7;295(32):11231-11

Insert

betaActin - CIB

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

cmv

Available since

Dec. 2, 2019

Availability

Academic Institutions and Nonprofits only

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ActinS14V.CIB.GFP

Plasmid

#133620

Purpose

Expresses beta actin S14V - CIBN - GFP protein fusion

Depositor

Robert Hughes

Article

Salem et al J Biol Chem. 2020 Aug 7;295(32):11231-11

Insert

betaActin - CIB

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

cmv

Available since

Dec. 2, 2019

Availability

Academic Institutions and Nonprofits only

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Dok1-EGFP

Plasmid

#174194

Purpose

Expresses Dok1 in mammalian cells

Depositor

Cheng-han Yu

Article

Yu et al Nat Commun. 2015 Oct 28;6:8672. doi: 10.

Insert

DOK1 (DOK1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 16, 2021

Availability

Academic Institutions and Nonprofits only

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RGG-mCherry-RGG-eDHFR

Plasmid

#164648

Purpose

Two RGG domains tagged with mCherry and eDHFR

Depositor

Huaiying Zhang, Michael Lampson

Article

Zhang et al Mol Biol Cell. 2020 Aug 15;31(18):2048-2

Insert

RGG

Use

Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

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pLEX-FHH-MAPKAP1RBD/Triple Mutant

Plasmid

#120711

Purpose

Expresses Flag-HA-6xHIS-MAPKAP1 RBD Triple Mutant fusion protein in mammalian cells & for virus production

Depositor

Paul Khavari

Article

Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

Insert

MAPKAP1 RBD

Use

Lentiviral

Tags

Flag-HA-6xHIS

Expression

Mammalian

Mutation

Amino acids 279-353, K307A, K310A, R312L

Promoter

CMV

Available since

Jan. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pTargeting_Mettl3C_FKBP-V

Plasmid

#165421

Purpose

Template for knockin FBKP-V into Mettl3 C-terminus

Depositor

Neil Brockdorff

Article

Wei et al Genome Res. 2021 Aug;31(8):1395-1408. do

Insert

Mettl3 C-terminus + linker + FKBP-V (Mettl3 Mouse)

Use

Mouse Targeting

Tags

FKBP-V

Expression

Mutation

Promoter

Available since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pCK-D390TN(UniProt)-FLAG

Plasmid

#164693

Purpose

Express N-terminally truncated DroshaTN-FLAG in mammalian cells

Depositor

David Bartel

Article

Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

Insert

N-terminally truncated transdominant negative Drosha (DROSHA Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 25, 2021

Availability

Academic Institutions and Nonprofits only

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pCRI012-pGEM-PacI-PU3-BsmbI-NotI

Plasmid

#140204

Purpose

Vector for cloning Cas9 sgRNA ( Step 1 of 2-step cloning). Contains AfU3 promoter driven sgRNA expression cassette flanked by PacI and NotI for further cloning in fungal vector.

Depositor

Yit Heng Chooi

Article

Roux et al ACS Synth Biol. 2020 Jun 11. doi: 10.102

Type

Empty backbone

Use

CRISPR and Synthetic Biology; Step 1 of cloning c…

Tags

Expression

Mutation

Promoter

Aspergillus fumigatus U3 (RNAPIII).

Available since

Aug. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pBI-CMV1-mir451-CMV2-mir1

Plasmid

#164635

Purpose

Express miR-451 and miR-1-1 in mammalian cells

Depositor

David Bartel

Article

Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

Inserts

miR451a (MIR451A Human)
miR-1-1 (MIR1-1 )

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 15, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA5 FRT/TO Plk4 KD ∆24-mCherry

Plasmid

#80272

Purpose

mammalian expression plasmid for kinase dead Plk4 with multi-phospho degron deleted

Depositor

Andrew Holland

Article

Moyer et al J Cell Biol. 2015 Jun 22;209(6):863-78.

Insert

Plk4 (PLK4 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

deletion of multi-phospho degron (amino acids 282…

Promoter

CMV

Available since

Aug. 2, 2016

Availability

Academic Institutions and Nonprofits only

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