We narrowed to 15,050 results for: pET

1. pET28a-T4uvsW

   Plasmid

   #228201

   Purpose

   Expression in BL21(DE3) of T4 3' to 5' helicase/single-strand DNA annealase

   Depositor

   Stephen Benkovic

   Article

   Nelson et al J Biol Chem. 2007 Jan 5;282(1):407-16. d

   Insert

   uvsW
   

   Tags

   hexahistidine and 10 amino acid linker

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pETNb-nALFA

   Plasmid

   #159988

   Purpose

   For the protein expression with ALFA tag at N-terminus in E. coli

   Depositor

   Motoyuki Hattori

   Article

   Jin et al Commun Biol. 2021 Mar 19;4(1):366. doi:

   Type

   Empty backbone

   Tags

   MVKYI-8histidine-ALFA tag-HRV3C protease cleavage…

   Expression

   Bacterial

   Available Since

   Oct. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. NylB'_pET21b

   Plasmid

   #215416

   Purpose

   Expression construct for NylB' gene, codon optimized for expression in E. coli

   Depositor

   Gregg Beckham

   Article

   Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

   Insert

   NylB'
   

   Expression

   Bacterial

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET20b-mPTER

   Plasmid

   #222189

   Purpose

   Bacterial expression of mouse PTER protein

   Depositor

   Jonathan Long

   Article

   Wei et al bioRxiv [Preprint]. 2024 Mar 22:2024.03.

   Insert

   PTER (Pter Mouse)
   

   Tags

   6xHis and Strep

   Expression

   Bacterial

   Available Since

   Aug. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28b-NM23-H1

   Plasmid

   #157987

   Purpose

   Expresses NM23-H1 in E.coli cell

   Depositor

   Byung Il Lee

   Article

   Han et al Bulletin of the Kor Chem Soc 2010; 31(5)

   Insert

   NM23-H1 (NME1 Human)
   

   Expression

   Bacterial

   Available Since

   Aug. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCyPet-Rac1(WT)

   Plasmid

   #22782

   Depositor

   Klaus Hahn

   Article

   Machacek et al Nature. 2009 Sep 3. 461(7260):99-103.

   Insert

   Rac1 (wt) (RAC1 Human)
   

   Tags

   CyPet

   Expression

   Mammalian

   Available Since

   Jan. 22, 2010

   Availability

   Academic Institutions and Nonprofits only

7. pEThT-SAHS1

   Plasmid

   #90025

   Purpose

   To express His-tagged SAHS1 in bacteria

   Depositor

   Takekazu Kunieda

   Article

   Yamaguchi et al PLoS One. 2012;7(8):e44209.

   Insert

   SAHS1
   

   Tags

   His

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET24c(+)-EFTs

   Plasmid

   #31173

   Depositor

   Linda Spremulli

   Article

   Zhang et al FEBS Lett. 1996 Aug 12. 391(3):330-2.

   Insert

   E. coli elongation factor Ts
   

   Tags

   His

   Expression

   Bacterial

   Available Since

   Aug. 24, 2011

   Availability

   Academic Institutions and Nonprofits only

9. GSK3beta/pET29b

   Plasmid

   #16315

   Depositor

   Peter Klein

   Article

   Hedgepeth et al Dev Biol. 1997 May 1. 185(1):82-91.

   Insert

   GSK3beta (gsk3b.L Frog)
   

   Expression

   Bacterial

   Available Since

   Dec. 21, 2007

   Availability

   Academic Institutions and Nonprofits only

10. pET17b-yebST

    Plasmid

    #175804

    Purpose

    Complementation of yebST knockout in E. coli K-12

    Depositor

    Ian Henderson, Gira Bhabha, Damian Ekiert

    Article

    Isom et al Sci Rep. 2017 Aug 17;7(1):8608. doi: 10.

    Insert

    yebST
    

    Tags

    None

    Expression

    Bacterial

    Mutation

    silent mutations to introduce restriction sites

    Promoter

    T7

    Available Since

    April 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET-mF(3230)A3

    Plasmid

    #168016

    Purpose

    Expresses MBP-F(3230)A3-6xHis in bacterial cells

    Depositor

    David Liu

    Article

    Blum et al Science. 2021 Feb 19;371(6531):803-810.

    Insert

    MBP-F(3230)A3-6xHis
    

    Tags

    Hisx6 and maltose binding protein

    Expression

    Bacterial

    Available Since

    April 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pETM6-G6-vioABECD

    Plasmid

    #66536

    Purpose

    pETM6 vector containing vioA-vioB-vioE-vioC-vioD in monocistronic configuration with G6 promoter

    Depositor

    Mattheos Koffas

    Article

    Jones et al Sci Rep. 2015 Jun 11;5:11301. doi: 10.10

    Inserts

    VioA
    VioB
    VioE
    VioC
    VioD
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    Mutant T7 Promoter - G6

    Available Since

    July 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET14b-dabAB2

    Plasmid

    #133013

    Purpose

    pET14b carrying the dabA2 gene (Uniprot: D0KWS7) with a c-terminal strep tag fusion and the dabB2 gene (Uniprot: D0KWS8) with a c-terminal sfGFP V206K fusion and 6xHis tag

    Depositor

    David Savage

    Article

    Desmarais et al Nat Microbiol. 2019 Aug 12. pii: 10.1038

    Inserts

    dabB2
    dabA2
    

    Tags

    6xHis, StrepII tag, and sfGFP

    Expression

    Bacterial

    Promoter

    t7

    Available Since

    Nov. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET28GST-LIC

    Plasmid

    #26101

    Purpose

    SGC Empty backbone for bacterial expression

    Depositor

    Cheryl Arrowsmith

    Type

    Empty backbone

    Tags

    6XHis, GST, and Thrombin CLeavage Site

    Expression

    Bacterial

    Available Since

    Aug. 20, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pET32-sLatherin

    Plasmid

    #61685

    Purpose

    bacterial expression of the horse sweat/saliva protein Latherin as a His-tagged thioredoxin fusion

    Depositor

    Brian Smith

    Article

    Vance et al J R Soc Interface. 2013 Jun 19;10(85):20

    Insert

    Latherin (LATH horse)
    

    Tags

    HIS and Trx-Tag thioredoxin protein

    Expression

    Bacterial

    Mutation

    Codon optimized for E.coli

    Promoter

    T7

    Available Since

    March 2, 2015

    Availability

    Academic Institutions and Nonprofits only

16. pET15b/bPanK

    Plasmid

    #73619

    Purpose

    Produces Escherichia coli pantothenate kinase, with N-terminal His6 tag (H6PanK)

    Depositor

    Suzanne Jackowski, T. J. Kappock

    Article

    Calder et al J Biol Chem. 1999 Jan 22;274(4):2014-20.

    Insert

    pantothenate kinase (coaA Escherichia coli)
    

    Tags

    His6

    Expression

    Bacterial

    Mutation

    relative to MG1655 sequence, changes glutamate-5 …

    Promoter

    T7

    Available Since

    Feb. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pETcLIC-GFP

    Plasmid

    #183514

    Purpose

    The plasmid contains LIC cassette upstream of folding reporter GFP and TEV-cleavable 10x histidine purification tag. Compatible with T7 promoter-based expression system in E. coli

    Depositor

    Martin Högbom

    Article

    Grave et al Protein Expr Purif. 2022 Oct;198:106132.

    Type

    Empty backbone

    Tags

    10x His-tag and folding reporter GFP

    Expression

    Bacterial

    Promoter

    T7 lac

    Available Since

    June 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET28-2H12

    Plasmid

    #221186

    Purpose

    His6-tagged cdGreen expressed from T7 promoter (Internal Lab ID: UJ11308)

    Depositor

    Urs Jenal

    Article

    Kaczmarczyk et al Nat Commun. 2024 May 9;15(1):3920. doi:

    Insert

    cdGreen
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET30b-7Y-SrtA

    Plasmid

    #105601

    Purpose

    expresses 7Y sortase A variant in E.coli

    Depositor

    Stephanie Dougan

    Article

    Jeong et al PLoS One. 2017 Dec 4;12(12):e0189068. do

    Insert

    S.aureus Sortase A, 7Y mutant
    

    Tags

    His tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET29a-hNABP2

    Plasmid

    #128307

    Purpose

    Express hNABP2 (a.k.a hSSB1 or SOSSB1) in E. coli with a C-terminal His-tag

    Depositor

    Yuliang Wu

    Article

    Vidhyasagar et al Biochim Biophys Acta. 2016 Feb;1860(2):3

    Insert

    Human Nucleic Acid Binding Protein 2 (hNABP2) (NABP2 Human)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    Y85C- please see depositor comments below

    Promoter

    T7

    Available Since

    Aug. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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