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pRVGP-BB

Plasmid

#15380

Depositor

Stephen Smale

Article

Ramirez-Carrozzi et al Genes Dev. 2006 Feb 1. 20(3):282-96.

Insert

mU6-BRG/BRM shRNA (Smarca4, Smarca2 Mouse)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

April 18, 2008

Availability

Academic Institutions and Nonprofits only
ER50-SpCas9-ER50

Plasmid

#85448

Purpose

Expresses SpCas9 fused to ER50 domain on both N- and C-termini in mammalian cells

Depositor

Amit Choudhary

Article

Maji et al Nat Chem Biol. 2017 Jan;13(1):9-11. doi:

Insert

ER50-SpCas9-ER50

Use

AAV

Tags

destabilized domain of estrogen receptor ligand b…

Expression

Mammalian

Promoter

Cbh

Available Since

Feb. 9, 2017

Availability

Academic Institutions and Nonprofits only

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anti-Notch2_B6-pBIOCAM5

Plasmid

#39347

Depositor

John McCafferty

Article

Falk et al Methods. 2012 Sep;58(1):69-78. doi: 10.1

Insert

scFv-Fc-fusion specific for Notch2 (clone B6) (NOTCH2 Human)

Tags

3xFLAG, 6xHis, and human Fc

Expression

Mammalian

Available Since

Oct. 30, 2013

Availability

Academic Institutions and Nonprofits only

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HOXA10-MSCV

Plasmid

#20980

Depositor

Corey Largman

Article

Thorsteinsdottir et al Mol Cell Biol. 1997 Jan . 17(1):495-505.

Insert

HOXA10 (HOXA10 Human)

Use

Retroviral

Expression

Mammalian

Available Since

Aug. 14, 2009

Availability

Academic Institutions and Nonprofits only
pST_116_LVL2 cam

Plasmid

#179332

Purpose

NT-CRISPR plasmid for a single gRNA.

Depositor

Anke Becker

Article

Stukenberg et al Commun Biol. 2022 Mar 25;5(1):265. doi:

Insert

Ptac tfoX, Ptet cas9, PJ23106 acrIIA4, Ptet gRNA with sfGFP dropout

Use

CRISPR

Available Since

March 21, 2022

Availability

Academic Institutions and Nonprofits only

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SpyCatcher002-oTri

Plasmid

#124662

Purpose

Expresses SpyCatcher002-oTri for trimerization of SpyTag-proteins via trimeric coiled-coil

Depositor

Mark Howarth

Article

Khairil Anuar et al Nat Commun. 2019 Apr 15;10(1):1734. doi:

Insert

SpyCatcher002-oTri

Tags

C-tag, His6, and TEV cleavage site

Expression

Bacterial

Promoter

T7

Available Since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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pEG302 22aa SunTag NtDRMcd nog

Plasmid

#115488

Purpose

CRISPR-Cas9 SunTag system to target NtDRMcd to specific loci of interest (nog=no guide)

Depositor

Steven Jacobsen

Article

Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:

Insert

NOS_NLS_GB1_NLS_linker_DRMcd_linker sfGFP_scFv_UBQ10_Insulator_ UBQ10_Ω dCas9_1xHA_3xNLS_linker_10xGCN4_OCS

Use

CRISPR and Synthetic Biology

Expression

Plant

Available Since

March 17, 2019

Availability

Academic Institutions and Nonprofits only

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MAC_N_MED13

Plasmid

#111689

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

Insert

MED13 (MED13 Human)

Expression

Mammalian

Available Since

June 14, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Flag Erg

Plasmid

#66977

Purpose

Expression plasmid for mouse Erg; adds Flag tag to the N-terminus of protein

Depositor

Christopher Vakoc

Article

Roe et al Mol Cell. 2015 May 12. pii: S1097-2765(1

Insert

Erg (Erg Mouse)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

July 21, 2015

Availability

Academic Institutions and Nonprofits only
pFastbac1-GST-hUSP7

Plasmid

#63573

Purpose

Expression of synthetic human USP7 tagged with GST

Depositor

Titia Sixma

Article

Faesen et al Mol Cell. 2011 Oct 7;44(1):147-59. doi:

Insert

USP7 (USP7 Human, Synthetic)

Tags

GST

Expression

Insect

Mutation

Synthetic based on human protein sequence (please…

Promoter

Polyhedrin

Available Since

April 17, 2015

Availability

Academic Institutions and Nonprofits only

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tdTomato-anti-AlfaTag nanobody

Plasmid

#171819

Purpose

Bacterial expression vector for production of fluorescent anti-AlfaTag nanobody

Depositor

Ikuo Wada

Article

Matsumoto et al J Biol Chem. 2021 Jul;297(1):100851. doi

Insert

tdTomato-anti-AlfaTag nanobody

Tags

6xHis and SEP tag (6xD)

Expression

Bacterial

Available Since

July 12, 2022

Availability

Academic Institutions and Nonprofits only

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pJ4M_hnRNPA2_FL_WT

Plasmid

#139109

Purpose

Express FL hnRNPA2 with a C-terminal MBP tag

Depositor

Nicolas Fawzi

Article

Ryan et al Nucleic Acids Res. 2020 Sep 1. pii: 5900

Insert

hnRNPA2 (HNRNPA2B1 Human)

Expression

Bacterial

Mutation

none

Available Since

Aug. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pNW-sfGFP(SPS6)

Plasmid

#133814

Purpose

Fluorescent protein sfGFP(SPS6) variant for optimized expression in Bacillus mycoides.

Depositor

Oscar Kuipers

Article

Yi et al Environ Microbiol Rep. 2018 Feb;10(1):57

Insert

sfGFP(SPS6)

Use

E.coli/bacillus spp. shuttle vector

Expression

Bacterial

Promoter

Ppta

Available Since

Nov. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pMRBAD-link-CGFP

Plasmid

#52733

Purpose

L-(+)-arabinose-inducible expression of link-CGFP negative control for in vivo split GFP assembly assay

Depositor

Lynne Regan

Article

Magliery et al J Am Chem Soc. 2005 Jan 12;127(1):146-57

Insert

link-CGFP

Expression

Bacterial

Promoter

PBAD

Available Since

May 7, 2014

Availability

Academic Institutions and Nonprofits only

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MSCV p2GM AMPK alpha2hp1 alpha1hp1

Plasmid

#89492

Purpose

shRNA against AMPK alpha2 and alpha1 in mouse/human to knock down protein expression

Depositor

Russell Jones

Article

Faubert et al Cell Metab. 2013 Jan 8;17(1):113-24. doi

Insert

2 hairpins against mouse/human AMPKalpha1 and alpha2

Use

Retroviral

Expression

Mammalian

Promoter

U6 (The GFP-Puro is from a PGK promoter; the shRN…

Available Since

May 19, 2017

Availability

Academic Institutions and Nonprofits only
pSLIK 3xHA-MKK6-EE neo

Plasmid

#47546

Purpose

Lentiviral expression vector for an inducible 3xHA-tagged constitutively active MKK6 mutant

Depositor

Kevin Janes

Article

Jensen et al Cell Host Microbe. 2013 Jan 16;13(1):67-

Insert

MKK6-EE (MAP2K6 Human)

Use

Lentiviral

Tags

3XHA

Expression

Mammalian

Mutation

S207E, T211E

Available Since

Sept. 13, 2013

Availability

Academic Institutions and Nonprofits only

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pAAV-syn-flex-iGluSnFR4f-NGR-WPRE

Plasmid

#234442

Purpose

AAV-mediated expression of glutamate sensor with improved sensitivity and fast deactivation kinetics (4f = fast); NGR vector matches or outperforms PDGFR in all conditions tested.

Depositor

GENIE Project

Article

Aggarwal et al Nat Methods. 2026 Feb;23(2):417-425. doi

Has Service

AAV1

Insert

iGluSnFR4f-NGR

Use

AAV and Cre/Lox

Tags

IgK chain and Myc epi tag-NGR GPI anchor

Expression

Mammalian

Promoter

Synapsin

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV_hSyn1_nLightR

Plasmid

#187180

Purpose

Expresses red norepinephrine indicator nLightR in neuronal cells

Depositor

Tommaso Patriarchi

Article

Kagiampaki et al Nat Methods. 2023 Sep;20(9):1426-1436. d

Has Service

AAV9

Insert

nLightR

Use

AAV

Tags

Flag tag

Promoter

human Synapsin-1

Available Since

July 31, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-hsyn-GRAB_rDA-mut

Plasmid

#140558

Purpose

Expresses the red fluorescent DA-insensitive control sensor GRAB_rDA-mut in neurons

Depositor

Yulong Li

Article

Sun et al Nat Methods. 2020 Nov;17(11):1156-1166.

Has Service

AAV9

Insert

Red fluorescent DA-insensitive sensor GRAB_rDA-mut (control sensor)

Use

AAV

Promoter

hSyn

Available Since

July 29, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-oScarlet

Plasmid

#137135

Purpose

Viral expression of oScarlet

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Article

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Has Service

AAV8

Insert

oScarlet

Use

AAV

Mutation

E95D

Promoter

EF1a

Available Since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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